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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.28 no.3 São Paulo May/June 1986

http://dx.doi.org/10.1590/S0036-46651986000300002 

ARTIGOS ORIGINAIS

 

Interaccion de promastigotes de Leishmania donovani con celulas de exudado peritonial de ratones mediante la influencia de la quimotripsina

 

Interaction of Leishmania donovani promastigotes with mouse peritoneal exudate cells under the influence of the Chymotripsine

 

 

Lucila ArcayI; Elizabeth BruzualII

IInstituto de Zoologia Tropical, Universidad Central de Venezuela, Caracas, Venezuela
IIEscuela de Medicina "José Maria Vargas", Universidad Central Caracas, Venezuela

 

 


RESUMEN

Se hace un estudio de la interacción de promastigotes de Leishmania donovani con células de exudado peritoneal de ratón (c e p) mediante la influencia de la quimotripsina. La adhesión de los promastigotes a las c e p fue terminal y marginal, y en observaciones hechas a partir de los 10 minutos de enfrentamiento, esta adhesión fue nula hasta los 30 minutos en el grupo tratado, y sólo a las das horas hubo un pequeño incremento (2.4%) con respecto al control. Se observa marcada disminución en todos los parámetro medidos, tales como enlace, penetración, multiplicación intracelular, división de formas flageladas, en el grupo tratado. La quimotripsina favorece la formación de formas intermedias flageladas, heciendose el parásito piriforme y esférico, apareciendo una forma aberrante de extremo anterior cilindrico que semeja a una forma coanoflagelada. Se sospecha que la enzima reduce efectivamente fragmentos proteicos o péptidos, los cuales pueden haber sido tan pequeños como para esconder a otros ligandos relacionados con la adhesión macrófago-parásito.

Unitermos: Calazar — Leishmania donovani — Peritonio — Ratos.


SUMMARY

The interaction of promastigotes of Leishmania donovani with mouse peritoneal exudate cells (cep) under the influence of Chymotripsine was studied. The promastigote adhered to the cep terminally and marginally. At 10-30 minutes postchallange, adhesion was absent in the treated group, and only after two hours was there any adhesión (24% in comparison with the controls). The experimental group was markedly deficient in all the parameters of activity measured: attachment, penetration, intracellular multiplication and division of flagellates forms in comparison to controls. The Chymotripsine favored the development of intermediate flagellates forms, these being spheroid or pyriform, with example of an aberrant form having the anterior end cylindrical similar to a choanoflagellate. It is suspected that the Chymotripsine effectively reduces protein or peptide fragments; these may have been so small as to conceal other ligands associated with macrophage-parasite adhesion.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

AGRADECIMIENTO

Al histotecnólogo del Instituto de Zoologia Tropical de la Universidad Central de Venezuela (UCV) por su asistencia técnica y dibujo lineal de los gráficos. Al Consejo de Desarollo Cientifico y Humanístico de Ir UCV por la subvención del Proyecto.

 

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Recebido para publicação em 17/4/1985.

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