An immunohistochemic assay to localize leptospires in tissue specimens

Alves, Venâncio A. F.; Yasuda, Paulo H.; Yamashiro, Edite H.; Santos, Raimunda Telma M.; Yamamoto, Luzia Umeda; Brito, Thales de

doi:10.1590/S0036-46651986000300006

Abstracts

An Immunoperoxidase technique for identification of leptospires in formalin fixed, paraffin embedded kidney sections is presented, using peroxidase-antiperoxidase complex. The anti-leptospiral antibody was raised in rabbit. Possible applications of this technique are discussed.

Leptospirosis; Immunodiagnosis; Immunohistochemistry; Kidney Sections

Os autores apresentam uma técnica de imunoperoxidase para a identificação de leptospiras em cortes de rim fixados em formol e emblocados em parafina. A variante metodológica inclui a utilização do complexo peroxidase-antiperoxidase, conferindo alta sensibilidade à reação. São, ainda, discutidas algumas possíveis aplicações do método ao estudo do acometimento renal na leptospirose.

ORIGINAL ARTICLES

An immunohistochemic assay to localize leptospires in tissue specimens

Método imunohistoquímico para localização de leptospiras em amostras teciduais

Venâncio A. F. Alves^I, ^II; Paulo H. Yasuda^II; Edite H. Yamashiro^III; Raimunda Telma M. Santos^I; Luzia Umeda Yamamoto^I; Thales de Brito^{II, III}

^IInstitute Adolfo Lutz, Sao Paulo State Secretary of Health. P.O. Box 7027, São Paulo, Brasil

^IIFaculty of Medicine, São Paulo University

^IIIInstitute of Tropical Medicine, São Paulo

SUMMARY

An Immunoperoxidase technique for identification of leptospires in formalin fixed, paraffin embedded kidney sections is presented, using peroxidase-antiperoxidase complex. The anti-leptospiral antibody was raised in rabbit. Possible applications of this technique are discussed.

Key words: Leptospirosis Immunodiagnosis Immunohistochemistry Kidney Sections.

RESUMO

Os autores apresentam uma técnica de imunoperoxidase para a identificação de leptospiras em cortes de rim fixados em formol e emblocados em parafina. A variante metodológica inclui a utilização do complexo peroxidase-antiperoxidase, conferindo alta sensibilidade à reação. São, ainda, discutidas algumas possíveis aplicações do método ao estudo do acometimento renal na leptospirose.

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Recebido para publicação em 12/7/1985.

1. AREAN, V. M. The pathologic anatomy and pathogenesis of fatal human leptospirosis (Weil's disease). Amer. J. Path., 40: 393-423, 1962.
2. ARRIAGA, A. J. D.; ROCHA, A. S.; YASUDA, P. H. & DE BRITO, T. Morpho-functional patterns ot kidney injury in the experimental leptospirosis of the guinea-pig (Licterohaemorrhagiae). J. Path., 138: 145-161, 1962.
3. CAMARGO, M. C. C.; FIGUEIREDO, G. M.; VENEZIANI, P. & OKI, S. Leptospirose. Rev. paul. Med., 101: 74-76, 1983.
4. DE BRITO, T. On the pathogenesis of the hepatic and renal lesions in leptospirosis. Rev. Inst. Med. trop. S. Paulo, 10: 236-241, 1968.
5. DOOLEY, J. R. & ISHAK, K. G. Leptospirosis. In: BINPORD, C. H. & CONNOR, D. H., ed. Pathology of tropical and extraordinary diseases. Washington, D.C., Armed Forces Institute of Pathology, 1976. p. 101-106.
6. ELLIS, T. M.; ROBERTSON, G. M.; HUSTAS, L. & KIRBY, M. Detection of leptospires in tissue using an immunoperoxidase staining procedure. Aust. vet. J., 60: 364-367, 1963.
7. SITPRIJA, V., PlPATANAGUL, V.; MERTOWIDJOJO, K.; BOONPUCKNAVIG, V. & BOONPUCKNAVIG, S. Pathogenesis of renal disease in leptospirosis: clinical and experimental studies. Kidney Int., 17: 827-836, 1980.
8. STERNBERGER, L. A.; HARDY, P. H.; CUCULIS, J. J. & MEYER, H. G. The unlabeled antibody enzyme method of immunohistochemistry Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem., 18: 315-333, 1S70.
9. YASUDA, P. H.; HOSHINO-SHIMIZU, S.; YAMASHIRO, E. & DE BRITO, T. Experimental leptospirosis (L. interrogans, serovar Icterohaemorrhagiae) of the guinea pig: leptospiral antigen, gamma globulin and complement C₃ detection in the kidney. Exp. Path., 29: 35-43, 1986.

Publication Dates

Publication in this collection
16 Oct 2012
Date of issue
June 1986

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. AREAN, V. M. The pathologic anatomy and pathogenesis of fatal human leptospirosis (Weil's disease). Amer. J. Path., 40: 393-423, 1962.

[2] 2. ARRIAGA, A. J. D.; ROCHA, A. S.; YASUDA, P. H. & DE BRITO, T. Morpho-functional patterns ot kidney injury in the experimental leptospirosis of the guinea-pig (Licterohaemorrhagiae). J. Path., 138: 145-161, 1962.

[3] 3. CAMARGO, M. C. C.; FIGUEIREDO, G. M.; VENEZIANI, P. & OKI, S. Leptospirose. Rev. paul. Med., 101: 74-76, 1983.

[4] 4. DE BRITO, T. On the pathogenesis of the hepatic and renal lesions in leptospirosis. Rev. Inst. Med. trop. S. Paulo, 10: 236-241, 1968.

[5] 5. DOOLEY, J. R. & ISHAK, K. G. Leptospirosis. In: BINPORD, C. H. & CONNOR, D. H., ed. Pathology of tropical and extraordinary diseases. Washington, D.C., Armed Forces Institute of Pathology, 1976. p. 101-106.

[6] 6. ELLIS, T. M.; ROBERTSON, G. M.; HUSTAS, L. & KIRBY, M. Detection of leptospires in tissue using an immunoperoxidase staining procedure. Aust. vet. J., 60: 364-367, 1963.

[7] 7. SITPRIJA, V., PlPATANAGUL, V.; MERTOWIDJOJO, K.; BOONPUCKNAVIG, V. & BOONPUCKNAVIG, S. Pathogenesis of renal disease in leptospirosis: clinical and experimental studies. Kidney Int., 17: 827-836, 1980.

[8] 8. STERNBERGER, L. A.; HARDY, P. H.; CUCULIS, J. J. & MEYER, H. G. The unlabeled antibody enzyme method of immunohistochemistry Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem., 18: 315-333, 1S70.

[9] 9. YASUDA, P. H.; HOSHINO-SHIMIZU, S.; YAMASHIRO, E. & DE BRITO, T. Experimental leptospirosis (L. interrogans, serovar Icterohaemorrhagiae) of the guinea pig: leptospiral antigen, gamma globulin and complement C₃ detection in the kidney. Exp. Path., 29: 35-43, 1986.