Prevalence of anti-p: Falciparum sporozoite antibodies in adults in the amapa region of Brazil

Prevalência de anticorpos anti-esporozoitos de P: Falciparum em soros da região do Amapá, Brasil

EPIDEMIOLOGIA

Prevalence of anti-p. Falciparum sporozoite antibodies in adults in the amapa region of Brazil

Prevalência de anticorpos anti-esporozoitos de P. Falciparum em soros da região do Amapá, Brasil

Virgílio Do Rosario; Michael R. Hollingdale

Biomedical Research Institute, 1211 Parklawn Drive Rockville, Maryland 20852 USA

SUMMARY

17 of 20 adult sera from the Amapa region of Brazil were active in the inhibition of P. falciparum sporozoite invasion (ISI) assay which has been correlated with protective antibodies. In contrast 11 sera were positive in IFA tests and 6 were positive in CSP tests. These results suggest that the ISI assay will be useful for evaluating naturally acquired protective anti-sporozoite antibodies in endemic areas, particularly during vaccine efficacy studies using sporozoite-based vaccines.

Key-words: Anti-sporozoite antibody; inhibiton of sporozoite invasion (ISI) assay; circumsporozoite precipitation (CSP) assay; immunofluorescent antibody (IFA) assay.

RESUMO

17 de 20 soros humanos coletados na região de Amapá (Brasil) mostraram atividade nos testes de inibição de invasão de esporozoitos (ISI) in vitro que estão correlacionados com proteção por presença de anticorpos. Em contraste 11 soros foram positivos para IFA e 6 foram positivos para CSP. Estes resultados sugerem que ISI seja um ensaio mais adequado para medir respostas imunológicas em futuros estudos sobre eficácia de uma potencial vacina anti-malárica.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

ACKNOWLEDGEMENTS

The support and assistance of Dr. F. J. Lopez Antunano, Pan American Health Organization is gratefully appreciated. This study was supported in part by the United States Agency for International Development, Contract No DPE-0453-C-00-3051.

We thank the staff of the Institute Evandro Chagas (P. SESP), Malaria Research Unit for providing us with the sera samples.

Recebido para publicação em 18/06/86.

• 1. BALLOU, W. R.; ROTHARD, J.; WIRTZ, R. A.; GORDON, D. M.; WILLIAMS, J. S.; GORE, R. W.; SCHNEIDER, I.; HOLLINGDALE, M. R.; BEAUDOIN, R. L.; MELOY, W. L.; MILLER, L. H. & HOCKMEYER, W. T. - Immunogenicity and biologic activity of antibodies to synthetic peptides of circumsporozoite protein of P. falciparum. Science, 228: 996-999, 1985.
• 2. CLYDE, D.; MOST, H.; MCCARTHY, V. & VANDERBERG, J. - Immunization of man against sporozoite-induced falciparum malaria. Amer. J. med. Sci., 266: 169-177, 1973.
• 3. HOFFMAN, S. L.; WISTAR, R.; BALLOU, W. R.; HOLLINGDALE, M. R.; WIRTZ, R. A.; SCHNEIDER, I.; MARWOTO, H. A. & HOCKMEYER, W. T. - Naturally acquired human antibodies to Plasmodium falciparum circumsporozoite protein repeat region mediate biologic reactions associated with protective immunity. New Engl. J. Med. (in press)
• 4. HOLLINGDALE, M. R. & LELAND, P. - Detection of exoerythrocytic stages of Plasmodium berghei in liver tissue and cultures cells by an immunoperoxidase antibody technique. Trans, roy. Soc. trop. Med. Hyg., 76: 624-626, 1982.
• 5. HOLLINGDALE, M. R.; NARDIN, E. H.; THARAVANIJ, S.; SCHWARTZ, A. L. & NUSSENZWEIG, R. S. - Inhibition of entry of Plasmodium falciparum and P. vivax sporozoites into cultured cells: an in vitro assay of protective antibodies. J. Immunol., 132: 909-913, 1984.
• 6. NARDIN, E. H.; NUSSENZWEIG, R. S.; MCGREGOR, I. A. & BRYAN, J. H. - Antibodies to sporozoites: their frequent occurence in individuals living in an area of hyperendemic malaria. Science, 106: 597-599, 1979.
• 7. PONNUDURAI, T.; MEUWISSEN, J. H. E. T.; LEEUWENBERG, A. D. E. M.; VERHAVE, J. P. & LENSEN, A. H. W. - The production of mature gametocytes of Plasmodium falciparum in continuous culture of different isolates infective to mosquitoes. Trans, roy. Soc. trop. Med. Hyg., 76: 242-250, 1982.
• 8. SCHWARTZ, A. L. & HOLLINGDALE, M. R. -- Primaquine and lysosomotropic amines inhibit malaria sporozoite entry into human liver cells. Molec. Biochem. Parasit., 14: 305-311, 1985.
• 9. VANDERBERG, J.; NUSSENZWEIG, R. S. & MOST, H. - Protective immunity produced by infections of x-irradiated sporozoite of Plasmodium berghei V. In vitro effects of immune serum and sporozoites. Milit. Med., 134: 1183-1190, 1969.
• 10. YOUNG, J. F.; HOCKMEYER, W. T.; GROSS, M.; BALLOU, W. R.; WIRTZ, R. A.; TROSPER, J. H.; BEAUDOIN, R. L.; HOLLINGDALE, M. R.; MILLER, L. H.; DIGGS, C. L. & ROSENBERG, M. - Expression of P. falciparum circumsporozoite protein derivatives in E. coli for development of a human malaria vaccine. Science, 228: 958-962, 1985.
• 11. ZAVALA, F.; TAM, J. P.; HOLLINGDALE, M. R.; COCHRANE, A. H.; QUAKYI, I.; NUSSENZWEIG, R. S. & NUSSENZWEIG, V. - Rationale for development of a synthetic vaccine against Plasmodium falciparum*, malaria. Science, 228: 1436-1440, 1985.

Publication Dates

• Publication in this collection
  17 Feb 2011
• Date of issue
  Feb 1987

History

• Received
  18 June 1986