Schistosoma mansoni: implante de vermes na cavidade peritoneal visando imunoproteção

Coelho, Paulo Marcos Z.; Gerken, Sílvia E.; Tavares, Carlos Alberto P.

doi:10.1590/S0036-46651987000600005

Resumos

Tentou-se obter imunoproteção contra infecção cercariana pela inoculação de vermes vivos na cavidade peritoneal de camundongos. Embora os vermes sobrevivessem bem nestas condições e não ocorresse postura de ovos, não foi possível obter imunoproteção, Também a inoculação de ovos viáveis por via sangüínea e peritoneal não propiciou o aparecimento de imunoproteção nos camundongos com vermes na cavidade peritoneal.

Schistosoma mansoni; Implante de vermes; Cavidade peritoneal; Imunoproteção

An attempt has been made to induce immunoprotection against cercarial infection through inoculation of live worms into the peritoneal cavity of mice. Although the worms were able to survive normally under these conditions, and no egg-laying could be detected, immunoprotection was obtained. Likewise, the inoculation of viable eggs, by circulatory and peritoneal routes, did not provide immunoprotection in mice with worms in the peritoneal cavity.

ARTIGOS ORIGINAIS

Schistosoma mansoni: implante de vermes na cavidade peritoneal visando imunoproteção (^¹ (1 ) Trabalho executado com auxilio financeiro do CNPq e PINEP, Brasil. )

Schistosoma mansoni: implantation of worms into the peritoneal cavity aiming at the induduction of immunoprotection

Paulo Marcos Z. Coelho^I; Sílvia E. Gerken^I; Carlos Alberto P. Tavares^II

^IDepartamento de Parasitologia e Grupo Interdepartamental de Estudos sobre Esquistossomose (GIDE), ICB/UPMG

^IIDepartamento de Bioquímica Imunologia, ICB/UFMG

^{Endereço para correspondência} Endereço para correspondência: Prof. Paulo Marcos Z. Coelho GIDE-ICB/UFMG Caixa Postal 2486. CEP 30161 Belo Horizonte, MG., Brasil

RESUMO

Tentou-se obter imunoproteção contra infecção cercariana pela inoculação de vermes vivos na cavidade peritoneal de camundongos. Embora os vermes sobrevivessem bem nestas condições e não ocorresse postura de ovos, não foi possível obter imunoproteção, Também a inoculação de ovos viáveis por via sangüínea e peritoneal não propiciou o aparecimento de imunoproteção nos camundongos com vermes na cavidade peritoneal.

Unitermos:Schistosoma mansoni; Implante de vermes; Cavidade peritoneal; Imunoproteção.

SUMMARY

An attempt has been made to induce immunoprotection against cercarial infection through inoculation of live worms into the peritoneal cavity of mice. Although the worms were able to survive normally under these conditions, and no egg-laying could be detected, immunoprotection was obtained. Likewise, the inoculation of viable eggs, by circulatory and peritoneal routes, did not provide immunoprotection in mice with worms in the peritoneal cavity.

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Recebido para publicação em 9/3/1987.

1. BARBOSA, M. A.; PELLEGRINO, J.; COELHO, P. M. Z. & SAMPAIO, I. B. M. - Quantitative aspects of the migration and evolutive asynchronism of Schistosoma mansoni in mice. Rev. Inst. Med. trop. S. Paulo, 20: 121-132, 1978.
2. BICKLE, Q. D.; ANDREWS, B. J.; DOENHOFF, M. J.; FORD, M. J. & TAYLOR, M. G. - Resistance against Schistosoma mansoni induced by highly irradiated infections; studies on species specificity of immunization and attempts to transfer resistance. Parasitology, 90: 301-312, 1985.
3. BICKLE, Q. D.; BAIN, J.; McGREGOR, A. & DOENHOFF, M. - Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. III - The failure of primary infections with cercariae of one sex to induce resistance to reinfection. Trans. roy. Soc. trop. Med. Hyg., 73: 37-41, 1979.
4. COELHO, P. M. Z.; PELLEGRINO, J.; PEREIRA, L. H. & MELLO, R. T. - Migration of schistosomas (Schistosoma mansoni) collected from hamster and inoculated intraperitoneally in mice. Trans. roy. Soc, trop. Med. Hyg., 70: 161, 1976.
5. DEAN, D. A. - A review of Schistosoma and related general: acquired resistance in mice. Exp. Parasit., 55: 1-104, 1983.
6. DEAN, D. A.; BUKOWSKI, M. A. & CHEEVER, A. W. - Relationship between acquired resistance, portal hypertension and lung granuloma in ten strains of mice infected with Schistosoma mansoni Amer. J. trop. Med. Hyg., 30: 806-814, 1981a.
7. DEAN, D. A.; MANGOLD, B. L.; GEORGI, J. R. & JACOBSON, R. H. - Comparison of Schistosoma mansoni migration patterns in normal and irradiated cercaria-immunized mice by means of autoradiographic analysis; evidence that worm elimination occurs after the skin phase in immunized mico. Amer. J. trop. Med. Hyg., 33: 86-96, 1984.
8. DEAN, D. A.; MINARD, P.; STIREWALT, M. A.; VANNIER, W. E. & MURREL, K. D. - Resistance of mice to secondary infection with Schistosoma mansoni I. Comparison! of bisexual and unisexual initial infections. Amer. J. trop. Med. Hyg., 27: 951-956, 1978a.
9. GERKEN, S. E. - Schistosoma mansoni: Resistência cutânea de camundongos normais e portadores de infecção primária. Belo Horizonte, 1986. (Tese de Doutoramento - Dept.^ş de Parasitologia, ICB/UPMG).
10. GÓES, A. M. - Estudos in vivo dos mecanismos de imunidade protetora dependente de anticorpos que atuam na morte de esquistossômulos de Schistosoma mansoni em ratos Holtzman. Belo Horizonte, 1984. (Tese de doutoramento - Instituto de Ciências Bio-médicas da Universidade Federal de Minas Gerais).
11. HARRISON, R. A.; BICKLE, Q. & DOENHOFF, M. J. - Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. Eivid'ence that the mechanism which mediate resistance during early patent infections may lack immunological specificity. Parasitology, 84: 93-110, 1982.
12. HUNTER, G. W.; GRANDALL, R. B.; ZICKAFOOSE, D. E. & PURVIS, Q. B. - Studies on schistosomiasis. XVIII. Some factors affecting resistance to Schistosoma mansoni infections in albino mice. Amer. J. trop. Med. Hyg., 11: 17-24, 1962.
13. JAMES, S. L. & COLLEY, D. G. - A method for isolation of Schistosoma mansoni egg. J. Parasit., 60: 1043-1044, 19-4.
14. JAMES, S. L.; LUCAAS, S. B. & DOBINSON, A. R. - Pathology associated with vaccination against Schistosoma mansoni in mice using cryopreserved radiation-attenuated schistosomula. J. Helminth., 59: 51-55, 1985.
15. LEWIS, F. A.; HTENY, S. & SHER, A. - Evidence against the existence of specific Schstosoma mansoni subpopulations which are resistant to irradiated vaccine-induced immunity. Amer. J. trop. Med. Hyg., 34: 86-91, 1985.
16. LONG, E.; DOENHOFF, M. & BAIN, J. - Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. 2. The time at which resistance to reinfection develops. J. Helminth., 52: 87-191, 1978.
17. MANGOLD, B. L. & DEAN, D. A. - The migration and survival of gammairradiated Schistosoma mansoni larvae and the duration of host parasite contact in relation to induction of resistance in mice. Parasitology, 88: 249-266, 1984.
18. MASTIN, A. J.; BICKLE, Q. D. & WILSON, R. A. - Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse. Parasitology, 87: 87-102, 1983.
19. MhLAREN, D. J.; PEARCE, E. J. & SMITHERS, S. R. - Site potential for challenge attrition in mice, rats, and guinea pigs vaccinated with irradiated cercariae of Schistosoma mansoni Paras. Immunol., 7: 29-44, 1985.
20. MONGE, E.; COELHO, P. M. Z. & TAVARES, C. A. P. - Estúdios inmunologicos en hamsters (Cricetus auratus) infectados con Schistosoma masnoni. Rev. Inst. Med. trop. S. Paulo, 28: 239-245, 1986.
21. OLIVIER, L. & SCHNEIDMANN, M. - Acquired resistance to Schistosoma mansoni infection in laboratory animals. Amer. J. trop. Med. Hyg., 2: 289-306, 1953.
22. PELLEGRINO. J. & SIQUEIRA, A. F. - Técnica de perfusão para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.
23. PEREIRA, L. H.; COELHO, P. M. Z.; FONSECA, J. J. A.; BREDT, A. & PELLEGRINO, J. - Migration of Schistoosma mansoni larvae in the albino mouse. Rev. Inst. Med. trop. S. Paulo, 14: 306-309, 1972
24. PEREIRA, L. H.; PELLEGRINO, J.; MELLO, R. T.; VALADARES, T. E. & COELHO, P. M. Z. - New approach for the screening of antischistosomal agents on early developing forms of Schistosoma mansoni Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.
25. PEREZ, H. A.; CLEGG, J. Q. & SMITHERS, S. R. - Acquired imunity to Schistosoma mansoni in the rat: measurement of immunity by the lung recovery technique. Parasitology, 69: 349-359, 1974
26. SHER, A.; CORREA-OLIVEIRA, R.; HIENY, S. & HUSSAIN, R. - Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae. IV. Analysis of the role of immunoglobulins E antibodies and mast ctlls. J. Immunol., 131: 1460-1465, 1983.
27. SHER, A.; HIENY, S. & JAMES, S. - Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae. VI. Influence of the major histocompatibility complex. Paras. Immunol., 6: 319-328, 1984.
28. SIMPSON, A. J. G.; AMES, S. L. & SHER, A. - Identification of surface antigens of schistosomula of Schistosoma mansoni recognized by antibodies from mice immunized by chronic infection and by exposure to highly irradiated cercariae. Infect. Immun., 41: 591-597. 1983.
29. SMITH, M. A. & CLEGG, J. A. - Schistosoma mansoni: decay of resistance induced by gamma irradiated cercariae in the mouse. Trans. roy. Soc. trop. Med. Hyg., 8: 190-192, 1984.
30. SMITHERS, S. R. & TERRY, R. J. - Acquired resistance to experimental infection of Schistosoma mansoni in albino rat. Parasitology, 55: 611-717, 1965.
31. SMITHERS, S. R. &. TERRY, R. J. - Resistance to experimental infection with Schistosoma mansoni in rhesus monkey induced by transfer of adult worms. Trans. roy. Soc. trop. Med. Hyg., 61: 517-523, 1967.
32. SNEDECOR, G. W. & COCHRAN, W. G. - Statistical methods. Iowa, Iowa State University Press, 1967.
33. WARREN, K. S. - The immunopathogenesis of schistosomiasis: a multidisciplinary approach. Trans. roy. Soc. trop. Med. Hyg., 66: 417-434, 1972.
34. WILSON, R. A.; COULSON, P. S. & McHUGH, S. M. - A significant parte of "concomitant immunity" of mice to Schistosoma mansoni is the consequence of a leaky hepatic portal system, not immune killing. Paras. Immunol. 5: 595-601, 1983.

Endereço para correspondência:

Prof. Paulo Marcos Z. Coelho

GIDE-ICB/UFMG

Caixa Postal 2486. CEP 30161

Belo Horizonte, MG., Brasil

(1

) Trabalho executado com auxilio financeiro do CNPq e PINEP, Brasil.

Datas de Publicação

Publicação nesta coleção
17 Fev 2011
Data do Fascículo
Dez 1987

Histórico

Recebido
09 Mar 1987

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BARBOSA, M. A.; PELLEGRINO, J.; COELHO, P. M. Z. & SAMPAIO, I. B. M. - Quantitative aspects of the migration and evolutive asynchronism of Schistosoma mansoni in mice. Rev. Inst. Med. trop. S. Paulo, 20: 121-132, 1978.

[2] 2. BICKLE, Q. D.; ANDREWS, B. J.; DOENHOFF, M. J.; FORD, M. J. & TAYLOR, M. G. - Resistance against Schistosoma mansoni induced by highly irradiated infections; studies on species specificity of immunization and attempts to transfer resistance. Parasitology, 90: 301-312, 1985.

[3] 3. BICKLE, Q. D.; BAIN, J.; McGREGOR, A. & DOENHOFF, M. - Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. III - The failure of primary infections with cercariae of one sex to induce resistance to reinfection. Trans. roy. Soc. trop. Med. Hyg., 73: 37-41, 1979.

[4] 4. COELHO, P. M. Z.; PELLEGRINO, J.; PEREIRA, L. H. & MELLO, R. T. - Migration of schistosomas (Schistosoma mansoni) collected from hamster and inoculated intraperitoneally in mice. Trans. roy. Soc, trop. Med. Hyg., 70: 161, 1976.

[5] 5. DEAN, D. A. - A review of Schistosoma and related general: acquired resistance in mice. Exp. Parasit., 55: 1-104, 1983.

[6] 6. DEAN, D. A.; BUKOWSKI, M. A. & CHEEVER, A. W. - Relationship between acquired resistance, portal hypertension and lung granuloma in ten strains of mice infected with Schistosoma mansoni Amer. J. trop. Med. Hyg., 30: 806-814, 1981a.

[7] 7. DEAN, D. A.; MANGOLD, B. L.; GEORGI, J. R. & JACOBSON, R. H. - Comparison of Schistosoma mansoni migration patterns in normal and irradiated cercaria-immunized mice by means of autoradiographic analysis; evidence that worm elimination occurs after the skin phase in immunized mico. Amer. J. trop. Med. Hyg., 33: 86-96, 1984.

[8] 8. DEAN, D. A.; MINARD, P.; STIREWALT, M. A.; VANNIER, W. E. & MURREL, K. D. - Resistance of mice to secondary infection with Schistosoma mansoni I. Comparison! of bisexual and unisexual initial infections. Amer. J. trop. Med. Hyg., 27: 951-956, 1978a.

[9] 9. GERKEN, S. E. - Schistosoma mansoni: Resistência cutânea de camundongos normais e portadores de infecção primária. Belo Horizonte, 1986. (Tese de Doutoramento - Dept.^ş de Parasitologia, ICB/UPMG).

[10] 10. GÓES, A. M. - Estudos in vivo dos mecanismos de imunidade protetora dependente de anticorpos que atuam na morte de esquistossômulos de Schistosoma mansoni em ratos Holtzman. Belo Horizonte, 1984. (Tese de doutoramento - Instituto de Ciências Bio-médicas da Universidade Federal de Minas Gerais).

[11] 11. HARRISON, R. A.; BICKLE, Q. & DOENHOFF, M. J. - Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. Eivid'ence that the mechanism which mediate resistance during early patent infections may lack immunological specificity. Parasitology, 84: 93-110, 1982.

[12] 12. HUNTER, G. W.; GRANDALL, R. B.; ZICKAFOOSE, D. E. & PURVIS, Q. B. - Studies on schistosomiasis. XVIII. Some factors affecting resistance to Schistosoma mansoni infections in albino mice. Amer. J. trop. Med. Hyg., 11: 17-24, 1962.

[13] 13. JAMES, S. L. & COLLEY, D. G. - A method for isolation of Schistosoma mansoni egg. J. Parasit., 60: 1043-1044, 19-4.

[14] 14. JAMES, S. L.; LUCAAS, S. B. & DOBINSON, A. R. - Pathology associated with vaccination against Schistosoma mansoni in mice using cryopreserved radiation-attenuated schistosomula. J. Helminth., 59: 51-55, 1985.

[15] 15. LEWIS, F. A.; HTENY, S. & SHER, A. - Evidence against the existence of specific Schstosoma mansoni subpopulations which are resistant to irradiated vaccine-induced immunity. Amer. J. trop. Med. Hyg., 34: 86-91, 1985.

[16] 16. LONG, E.; DOENHOFF, M. & BAIN, J. - Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. 2. The time at which resistance to reinfection develops. J. Helminth., 52: 87-191, 1978.

[17] 17. MANGOLD, B. L. & DEAN, D. A. - The migration and survival of gammairradiated Schistosoma mansoni larvae and the duration of host parasite contact in relation to induction of resistance in mice. Parasitology, 88: 249-266, 1984.

[18] 18. MASTIN, A. J.; BICKLE, Q. D. & WILSON, R. A. - Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse. Parasitology, 87: 87-102, 1983.

[19] 19. MhLAREN, D. J.; PEARCE, E. J. & SMITHERS, S. R. - Site potential for challenge attrition in mice, rats, and guinea pigs vaccinated with irradiated cercariae of Schistosoma mansoni Paras. Immunol., 7: 29-44, 1985.

[20] 20. MONGE, E.; COELHO, P. M. Z. & TAVARES, C. A. P. - Estúdios inmunologicos en hamsters (Cricetus auratus) infectados con Schistosoma masnoni. Rev. Inst. Med. trop. S. Paulo, 28: 239-245, 1986.

[21] 21. OLIVIER, L. & SCHNEIDMANN, M. - Acquired resistance to Schistosoma mansoni infection in laboratory animals. Amer. J. trop. Med. Hyg., 2: 289-306, 1953.

[22] 22. PELLEGRINO. J. & SIQUEIRA, A. F. - Técnica de perfusão para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.

[23] 23. PEREIRA, L. H.; COELHO, P. M. Z.; FONSECA, J. J. A.; BREDT, A. & PELLEGRINO, J. - Migration of Schistoosma mansoni larvae in the albino mouse. Rev. Inst. Med. trop. S. Paulo, 14: 306-309, 1972

[24] 24. PEREIRA, L. H.; PELLEGRINO, J.; MELLO, R. T.; VALADARES, T. E. & COELHO, P. M. Z. - New approach for the screening of antischistosomal agents on early developing forms of Schistosoma mansoni Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.

[25] 25. PEREZ, H. A.; CLEGG, J. Q. & SMITHERS, S. R. - Acquired imunity to Schistosoma mansoni in the rat: measurement of immunity by the lung recovery technique. Parasitology, 69: 349-359, 1974

[26] 26. SHER, A.; CORREA-OLIVEIRA, R.; HIENY, S. & HUSSAIN, R. - Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae. IV. Analysis of the role of immunoglobulins E antibodies and mast ctlls. J. Immunol., 131: 1460-1465, 1983.

[27] 27. SHER, A.; HIENY, S. & JAMES, S. - Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae. VI. Influence of the major histocompatibility complex. Paras. Immunol., 6: 319-328, 1984.

[28] 28. SIMPSON, A. J. G.; AMES, S. L. & SHER, A. - Identification of surface antigens of schistosomula of Schistosoma mansoni recognized by antibodies from mice immunized by chronic infection and by exposure to highly irradiated cercariae. Infect. Immun., 41: 591-597. 1983.

[29] 29. SMITH, M. A. & CLEGG, J. A. - Schistosoma mansoni: decay of resistance induced by gamma irradiated cercariae in the mouse. Trans. roy. Soc. trop. Med. Hyg., 8: 190-192, 1984.

[30] 30. SMITHERS, S. R. & TERRY, R. J. - Acquired resistance to experimental infection of Schistosoma mansoni in albino rat. Parasitology, 55: 611-717, 1965.

[31] 31. SMITHERS, S. R. &. TERRY, R. J. - Resistance to experimental infection with Schistosoma mansoni in rhesus monkey induced by transfer of adult worms. Trans. roy. Soc. trop. Med. Hyg., 61: 517-523, 1967.

[32] 32. SNEDECOR, G. W. & COCHRAN, W. G. - Statistical methods. Iowa, Iowa State University Press, 1967.

[33] 33. WARREN, K. S. - The immunopathogenesis of schistosomiasis: a multidisciplinary approach. Trans. roy. Soc. trop. Med. Hyg., 66: 417-434, 1972.

[34] 34. WILSON, R. A.; COULSON, P. S. & McHUGH, S. M. - A significant parte of "concomitant immunity" of mice to Schistosoma mansoni is the consequence of a leaky hepatic portal system, not immune killing. Paras. Immunol. 5: 595-601, 1983.

Brasil

Brasil

Schistosoma mansoni: implante de vermes na cavidade peritoneal visando imunoproteção

Schistosoma mansoni: implantation of worms into the peritoneal cavity aiming at the induduction of immunoprotection

Resumos

Datas de Publicação

Histórico