Strain variation in the infectivity of Schistosoma mansoni for Biomphalaria glabrata

Dias, Luiz Candido de Souza; Bruce, John I.; Coles, Gerald C.

doi:10.1590/S0036-46651988000200006

Abstracts

Five strains of Schistosoma mansoni resistant and susceptible to schistosomicides were studied for infectivity of 2 strains of Biomphalaria glabrata one of Puerto Rican origin and the other of Brazilian origin. Puerto Rican strains of S. Mansoni developed more slowly and had a lower infectivity in Brazilian B. glabrata than did the Brazilian S. mansoni. However, Brazilian S. Mansoni developed as well in Puerto Rican snails as in Brazilian snails, indicating that drug resistant strains could easily be moved by travel of infected persons from one area to another.

Schistosoma mansoni; Biomphalaria glabrata; Strains; Experimental infectivity

Com duas linhagens de Biomphalaria glabrata foi estudada a suscetibilidade de cinco cepas de Schistosoma mansoni resistentes e suscetíveis a esquistossomicidas. Três cepas do trematódeo oriundas de Porto Rico apresentaram desenvolvimento mais lento e menor índice de infecção em B. glabrata brasileira quando comparados com o comportamento de duas cepas de S. Mansoni provenientes do Brasil. Por outro lado, as cepas brasileiras do parasita desenvolve ram bem e infectaram mais de 90% dos exemplares de B. glabrata portorriquenhos. Entre os resultados, ressalta-se que cepas resistentes a esquistossomicidas poderão ser introduzidas por pacientes em diferentes áreas geográficas como Brasil e Porto Rico.

ORIGINAL ARTICLES

Strain variation in the infectivity of Schistosoma mansoni for Biomphalaria glabrata(^¹ (1 ) This study was supported in part by the Research Foundation of São Paulo State (FAPESP), Brazil and NIAID Grant n°1R21-Al-20171-01 to the Center for Tropical Diseases. University of Lowell, Lowell, Massachusetts, U.S.A. )

Suscetibilidade de linhagens de Biomphalaria glabrata a cepas de Schistosoma mansoni

Luiz Candido de Souza DiasI; John I. BruceII; Gerald C. ColesIII

^IState University of Campinas, Department of Parasitology. CP 6109. CEP 13081, Campinas, São Paulo, Brazil

^IICenter for Tropical Diseases, College of Pure and Applied Science, University of Lowell, Lowell, Massachusetts 01854, U.S.A

^IIIDepartment of Zoology, University of Massachusetts, Amherst, Massachusetts 01003, U.S.A

SUMMARY

Five strains of Schistosoma mansoni resistant and susceptible to schistosomicides were studied for infectivity of 2 strains of Biomphalaria glabrata one of Puerto Rican origin and the other of Brazilian origin.

Puerto Rican strains of S. Mansoni developed more slowly and had a lower infectivity in Brazilian B. glabrata than did the Brazilian S. mansoni. However, Brazilian S. Mansoni developed as well in Puerto Rican snails as in Brazilian snails, indicating that drug resistant strains could easily be moved by travel of infected persons from one area to another.

Key words:Schistosoma mansoni; Biomphalaria glabrata; Strains, Experimental infectivity.

RESUMO

Com duas linhagens de Biomphalaria glabrata foi estudada a suscetibilidade de cinco cepas de Schistosoma mansoni resistentes e suscetíveis a esquistossomicidas. Três cepas do trematódeo oriundas de Porto Rico apresentaram desenvolvimento mais lento e menor índice de infecção em B. glabrata brasileira quando comparados com o comportamento de duas cepas de S. Mansoni provenientes do Brasil. Por outro lado, as cepas brasileiras do parasita desenvolve ram bem e infectaram mais de 90% dos exemplares de B. glabrata portorriquenhos. Entre os resultados, ressalta-se que cepas resistentes a esquistossomicidas poderão ser introduzidas por pacientes em diferentes áreas geográficas como Brasil e Porto Rico.

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ACKNOWLEDGEMENTS

We are grateful to Dr. Yung-san Liang for his helpful suggestions and to Mr. David A. Boyd and Ms. Deborah M. Russell for their valuable technical assistance, and to Ms. Allison M. Howard for typing the manuscript. Support for this study was provided in part by the Research Foundation of São Paulo State (FAPESP), Brazil and by Grant Nv 1R21-AI-20171-01 from the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), United States of America.

REFERENCES

1. BRUCE, J. I. & RADKE, M - Culturing Biomphalaria and Oncomelania (Gastropoda) for large scale studies of schistoso miasis. I. Cultivation of Biomphalaria glabrata and maintenance of Schistosoma mansoni in the laboratory. Bio-Medical Reports of the 406th Medical Laboratory, 19: 1-84, 1971.

2. BASCH, P. F. - Intermediate host specificity in Schistosoma mansoni. Exp. Parasit. 39: 150-169, 1976.

3. COLES, G. C. - Alteration of Schistosoma mansoni malate dehydrogenase isoenzymes on passage in the laboratory. Comp. Biochem. Physiol., 40B: 1079-1083, 1971.

4. COLES, G. C. - Anthelmintic resistance in sheep. In: HUD, R. P.; SIBBS, H. C. & MURRELL, K. D., ed. - Veterinarian clinics of North America, food animal practices. Philadelphia, W. B. Saunders, 1986. v. 2. p. 423-432.

5. COLES, G. C. & BRUCE, J. I. - In vitro selection of drugresistant Schistosoma mansoni. Int. J. Parasit., 17: 767-771, 1987.

6. CRAM, E. B. - Evidence concerning geographic strains of human schistosomes and their molluscan hosts. Thapar Commemoration Volume. Lucknow, University of Lucknow, 1953. p. 51-62.

7. DIAS, L. C. S.; PEDRO, R. J.; RIGO, E.; GOTO, M. M. F. & MAFRA, G. L. - Linhagem humana de Schistosoma mansoni resistente a esquistossomicidas. Rev. Saúde publ. (S. Paulo), 12: 110, 1978.

8. JANSMA, W. B.; ROGERS, S. H.; LIU, C C. & BUEDING, E. - Experimentally produced resistance of Schistosoma mansoni to hycanthone. Amer. J. trop. Med. Hyg., 26: 926-936, 1977.

9. LIANG, Y. S. - Cultivation of Bulinus (Physopsis) globosus (Moreleti and Biomphalaria pfeifferi pfeifferi (Krauss), snail hosts of schistosomiasis. Sterkiana, 7: 53, 54, 1974.

10. LIANG, Y. S.; BOYD, D. A. & BRUCE. J. I. - Maintenance of Schistosome Cycles (Schistosoma mansoni, S. haematobium and S. japonicum). Unpublished.

11. LOVERDE, P. T.; DEWALD, J.; MINCHELLA, D. J.; BOS SHARDT, S. C. & DAMIAN, R. T. - Evidence for host in duced selection in Schistosoma mansoni. J. Parasit., 71: 297-300, 1985.

12. MICHELSON, E. A. & DUBOIS, L. - Susceptibility of Bahian populations of Biomphalaria glabrata to an allopatric strain of Schistosoma mansoni. Amer. J. trop. Med. Hyg., 27: 782-786, 1978.

13. MICHELSON, E A. & DUBOIS, L. - An isoenzyme marker possibly associated with the susceptibility of Biomphalaria glabrata populations to Schistosoma mansoni. Acta trop. (Basel), 38: 419-426, 1981.

14. MULVEY, M. & VRIJENHOEK, R. C. - Genetic variation among laboratory strains of the planorbid snail Biomphalaria glabrata. Biochem. Genet., 19: 1169-1182, 1981.

15. NEWTON, W. L. - The establishment of a strain of Australorbis glabratus which combines albinism and high susceptibility to infection with Schistosoma mansoni. J. Parasit., 41: 526-528, 1955.

16. PARAENSE, W. L. & CORREA, L. R. - Variation in susceptibility of Australorbis glabratus to a strain of Schistosoma mansoni. Rev. Inst. Med. trop. S. Paulo, 5: 15 22, 1963.

17. RICHARDS, C. S. - Susceptibility of adult Biomphalaria glabrata to Schistosoma mansoni infection Amer. J. trop. Med. Hyg., 22: 748-756, 1973.

18. RICHARDS, C. S. - Genetic factors in susceptibility of Biomphalaria glabrata for different strains of Schistosoma mansoni. Parasitology, 70: 231-241, 1975.

19. RICHARDS, C. S. & MERRITT JR., J. W. - Genetic factors in the susceptibility of juvenile Biomphalaria glabrata to Schistosoma mansoni infection Amer. J. trop. Med. Hyg, 21: 425-434, 1972.

20. VOGEL, H. - Infektionsversuche an verschiedenen Bilharzia Zwischenwirten mit einem einzelnen Mirazidium von Bilharzia mansoni und B. japonica. Zbl. Bakt., 148: 29-35. 1941.

21. WRIGHT, C. A. - Relationships between schistosomes and their molluscan hosts in Africa. J. Helminth., 40: 403-412, 1966.

22. WRIGHT, C. A.; FILE, S. K. & ROSS, G. C. - Studies on the enzyme systems of planorbid snails. Ann. trop. Med. Parasit., 60: 522-525, 1966.

Recebido para publicação em 21/12/1987.

1. BRUCE, J. I. & RADKE, M - Culturing Biomphalaria and Oncomelania (Gastropoda) for large scale studies of schistoso miasis. I. Cultivation of Biomphalaria glabrata and maintenance of Schistosoma mansoni in the laboratory. Bio-Medical Reports of the 406th Medical Laboratory, 19: 1-84, 1971.
2. BASCH, P. F. - Intermediate host specificity in Schistosoma mansoni Exp. Parasit. 39: 150-169, 1976.
3. COLES, G. C. - Alteration of Schistosoma mansoni malate dehydrogenase isoenzymes on passage in the laboratory. Comp. Biochem. Physiol., 40B: 1079-1083, 1971.
4. COLES, G. C. - Anthelmintic resistance in sheep. In: HUD, R. P.; SIBBS, H. C. & MURRELL, K. D., ed. - Veterinarian clinics of North America, food animal practices. Philadelphia, W. B. Saunders, 1986. v. 2. p. 423-432.
5. COLES, G. C. & BRUCE, J. I. - In vitro selection of drugresistant Schistosoma mansoni Int. J. Parasit., 17: 767-771, 1987.
6. CRAM, E. B. - Evidence concerning geographic strains of human schistosomes and their molluscan hosts. Thapar Commemoration Volume. Lucknow, University of Lucknow, 1953. p. 51-62.
7. DIAS, L. C. S.; PEDRO, R. J.; RIGO, E.; GOTO, M. M. F. & MAFRA, G. L. - Linhagem humana de Schistosoma mansoni resistente a esquistossomicidas. Rev. Saúde publ. (S. Paulo), 12: 110, 1978.
8. JANSMA, W. B.; ROGERS, S. H.; LIU, C C. & BUEDING, E. - Experimentally produced resistance of Schistosoma mansoni to hycanthone. Amer. J. trop. Med. Hyg., 26: 926-936, 1977.
9. LIANG, Y. S. - Cultivation of Bulinus (Physopsis) globosus (Moreleti and Biomphalaria pfeifferi pfeifferi (Krauss), snail hosts of schistosomiasis. Sterkiana, 7: 53, 54, 1974.
10. LIANG, Y. S.; BOYD, D. A. & BRUCE. J. I. - Maintenance of Schistosome Cycles (Schistosoma mansoni, S. haematobium and S. japonicum). Unpublished.
11. LOVERDE, P. T.; DEWALD, J.; MINCHELLA, D. J.; BOS SHARDT, S. C. & DAMIAN, R. T. - Evidence for host in duced selection in Schistosoma mansoni J. Parasit., 71: 297-300, 1985.
12. MICHELSON, E. A. & DUBOIS, L. - Susceptibility of Bahian populations of Biomphalaria glabrata to an allopatric strain of Schistosoma mansoni Amer. J. trop. Med. Hyg., 27: 782-786, 1978.
13. MICHELSON, E A. & DUBOIS, L. - An isoenzyme marker possibly associated with the susceptibility of Biomphalaria glabrata populations to Schistosoma mansoni Acta trop. (Basel), 38: 419-426, 1981.
14. MULVEY, M. & VRIJENHOEK, R. C. - Genetic variation among laboratory strains of the planorbid snail Biomphalaria glabrata Biochem. Genet., 19: 1169-1182, 1981.
15. NEWTON, W. L. - The establishment of a strain of Australorbis glabratus which combines albinism and high susceptibility to infection with Schistosoma mansoni. J. Parasit., 41: 526-528, 1955.
16. PARAENSE, W. L. & CORREA, L. R. - Variation in susceptibility of Australorbis glabratus to a strain of Schistosoma mansoni Rev. Inst. Med. trop. S. Paulo, 5: 15 22, 1963.
17. RICHARDS, C. S. - Susceptibility of adult Biomphalaria glabrata to Schistosoma mansoni infection Amer. J. trop. Med. Hyg., 22: 748-756, 1973.
18. RICHARDS, C. S. - Genetic factors in susceptibility of Biomphalaria glabrata for different strains of Schistosoma mansoni Parasitology, 70: 231-241, 1975.
19. RICHARDS, C. S. & MERRITT JR., J. W. - Genetic factors in the susceptibility of juvenile Biomphalaria glabrata to Schistosoma mansoni infection Amer. J. trop. Med. Hyg, 21: 425-434, 1972.
20. VOGEL, H. - Infektionsversuche an verschiedenen Bilharzia Zwischenwirten mit einem einzelnen Mirazidium von Bilharzia mansoni und B. japonica Zbl. Bakt., 148: 29-35. 1941.
21. WRIGHT, C. A. - Relationships between schistosomes and their molluscan hosts in Africa. J. Helminth., 40: 403-412, 1966.
22. WRIGHT, C. A.; FILE, S. K. & ROSS, G. C. - Studies on the enzyme systems of planorbid snails. Ann. trop. Med. Parasit., 60: 522-525, 1966.

(1

) This study was supported in part by the Research Foundation of São Paulo State (FAPESP), Brazil and NIAID Grant n°1R21-Al-20171-01 to the Center for Tropical Diseases. University of Lowell, Lowell, Massachusetts, U.S.A.

Publication Dates

Publication in this collection
17 Feb 2011
Date of issue
Apr 1988

History

Received
21 Dec 1987

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BRUCE, J. I. & RADKE, M - Culturing Biomphalaria and Oncomelania (Gastropoda) for large scale studies of schistoso miasis. I. Cultivation of Biomphalaria glabrata and maintenance of Schistosoma mansoni in the laboratory. Bio-Medical Reports of the 406th Medical Laboratory, 19: 1-84, 1971.

[2] 2. BASCH, P. F. - Intermediate host specificity in Schistosoma mansoni Exp. Parasit. 39: 150-169, 1976.

[3] 3. COLES, G. C. - Alteration of Schistosoma mansoni malate dehydrogenase isoenzymes on passage in the laboratory. Comp. Biochem. Physiol., 40B: 1079-1083, 1971.

[4] 4. COLES, G. C. - Anthelmintic resistance in sheep. In: HUD, R. P.; SIBBS, H. C. & MURRELL, K. D., ed. - Veterinarian clinics of North America, food animal practices. Philadelphia, W. B. Saunders, 1986. v. 2. p. 423-432.

[5] 5. COLES, G. C. & BRUCE, J. I. - In vitro selection of drugresistant Schistosoma mansoni Int. J. Parasit., 17: 767-771, 1987.

[6] 6. CRAM, E. B. - Evidence concerning geographic strains of human schistosomes and their molluscan hosts. Thapar Commemoration Volume. Lucknow, University of Lucknow, 1953. p. 51-62.

[7] 7. DIAS, L. C. S.; PEDRO, R. J.; RIGO, E.; GOTO, M. M. F. & MAFRA, G. L. - Linhagem humana de Schistosoma mansoni resistente a esquistossomicidas. Rev. Saúde publ. (S. Paulo), 12: 110, 1978.

[8] 8. JANSMA, W. B.; ROGERS, S. H.; LIU, C C. & BUEDING, E. - Experimentally produced resistance of Schistosoma mansoni to hycanthone. Amer. J. trop. Med. Hyg., 26: 926-936, 1977.

[9] 9. LIANG, Y. S. - Cultivation of Bulinus (Physopsis) globosus (Moreleti and Biomphalaria pfeifferi pfeifferi (Krauss), snail hosts of schistosomiasis. Sterkiana, 7: 53, 54, 1974.

[10] 10. LIANG, Y. S.; BOYD, D. A. & BRUCE. J. I. - Maintenance of Schistosome Cycles (Schistosoma mansoni, S. haematobium and S. japonicum). Unpublished.

[11] 11. LOVERDE, P. T.; DEWALD, J.; MINCHELLA, D. J.; BOS SHARDT, S. C. & DAMIAN, R. T. - Evidence for host in duced selection in Schistosoma mansoni J. Parasit., 71: 297-300, 1985.

[12] 12. MICHELSON, E. A. & DUBOIS, L. - Susceptibility of Bahian populations of Biomphalaria glabrata to an allopatric strain of Schistosoma mansoni Amer. J. trop. Med. Hyg., 27: 782-786, 1978.

[13] 13. MICHELSON, E A. & DUBOIS, L. - An isoenzyme marker possibly associated with the susceptibility of Biomphalaria glabrata populations to Schistosoma mansoni Acta trop. (Basel), 38: 419-426, 1981.

[14] 14. MULVEY, M. & VRIJENHOEK, R. C. - Genetic variation among laboratory strains of the planorbid snail Biomphalaria glabrata Biochem. Genet., 19: 1169-1182, 1981.

[15] 15. NEWTON, W. L. - The establishment of a strain of Australorbis glabratus which combines albinism and high susceptibility to infection with Schistosoma mansoni. J. Parasit., 41: 526-528, 1955.

[16] 16. PARAENSE, W. L. & CORREA, L. R. - Variation in susceptibility of Australorbis glabratus to a strain of Schistosoma mansoni Rev. Inst. Med. trop. S. Paulo, 5: 15 22, 1963.

[17] 17. RICHARDS, C. S. - Susceptibility of adult Biomphalaria glabrata to Schistosoma mansoni infection Amer. J. trop. Med. Hyg., 22: 748-756, 1973.

[18] 18. RICHARDS, C. S. - Genetic factors in susceptibility of Biomphalaria glabrata for different strains of Schistosoma mansoni Parasitology, 70: 231-241, 1975.

[19] 19. RICHARDS, C. S. & MERRITT JR., J. W. - Genetic factors in the susceptibility of juvenile Biomphalaria glabrata to Schistosoma mansoni infection Amer. J. trop. Med. Hyg, 21: 425-434, 1972.

[20] 20. VOGEL, H. - Infektionsversuche an verschiedenen Bilharzia Zwischenwirten mit einem einzelnen Mirazidium von Bilharzia mansoni und B. japonica Zbl. Bakt., 148: 29-35. 1941.

[21] 21. WRIGHT, C. A. - Relationships between schistosomes and their molluscan hosts in Africa. J. Helminth., 40: 403-412, 1966.

[22] 22. WRIGHT, C. A.; FILE, S. K. & ROSS, G. C. - Studies on the enzyme systems of planorbid snails. Ann. trop. Med. Parasit., 60: 522-525, 1966.

Brasil

Brasil

Strain variation in the infectivity of Schistosoma mansoni for Biomphalaria glabrata

Suscetibilidade de linhagens de Biomphalaria glabrata a cepas de Schistosoma mansoni

Abstracts

Publication Dates

History