Cryptosporidiosis among patients with acquired immunodeficiency syndrome (AIDS) in the county of São Paulo, Brazil

Criptosporidiose em pacientes acometidos pela síndrome de imunodeficiência adquirida (AIDS), no município de São Paulo, Brasil

EPIDEMIOLOGY

Cryptosporidiosis among patients with acquired immunodeficiency syndrome (AIDS) in the county of São Paulo, Brazil

Criptosporidiose em pacientes acometidos pela síndrome de imunodeficiência adquirida (AIDS), no município de São Paulo, Brasil

Rosa Maria Donini Souza Dias^I; Ana Célia Steffen Mangini^I; Domingas Maria A. Grispino Vieira Torres^I; Marcelo Oswaldo A. Corrêa^I; Norival Lupetti^I; Fernando M. A. Corrêa^II; Pedro Paulo ChieffI^I

^IFrom Instituto Adolf Lutz, São Paulo, Brazil

^IIFrom Instituto Butantan, São Paulo, Brazil

^{Address for correspondence}

SUMMARY

Stool samples of 157 patients with AIDS, living in the county of São Paulo, were submitted to several techniques in the search for Cryptosporidium sp.. Among the various techniques tested for slide preparation (direct smear, spontaneous sedimentation method, and formol-ether concentration), the latter, formol-ether concentration, offered the best results, clearly outdoing all the others. Nineteen samples out of 157 prepared by this technique, after dyeing by the Kinyoun method or by carbol fuchsin dimethyl sulfoxide, were found to be positive for Cryptosporidium sp..

Key words:Cryptosporidium sp.; Cryptosporidiosis; AIDS.

RESUMO

Amostras de fezes de 157 pacientes acometidos pela síndrome de imunodeficiência adquirida, residentes no município de São Paulo, foram submetidas a diversas técnicas para pesquisa de Cryptosporidium sp.. Entre as diversas técnicas testadas para o preparo das lâminas (esfregaço direto, método de sedimentação espontânea e concentração pelo formol-éter) a concentração pelo formol-éter foi a que forneceu melhores resultados, superando nitidamente as demais. Nas 157 amostras preparadas por esta técnica, após coloração pelo método de Kinyoun ou pela fucsinacarbólica dimetilsulfóxido, encontraram-se 19 (12,1%) positivas para oocistos de Cryptosporidium sp..

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Recebido para publicação em 22/3/1988.

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Address for correspondence:

Dra. Rosa Maria D. Souza Dias

Instituto Adolfo Lutz

Seção de Enteroparasitoses

Av. Dr. Arnaldo, 355

CEP 01246 São Paulo, SP, Brazil

Publication Dates

• Publication in this collection
  10 Mar 2011
• Date of issue
  Aug 1988