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Revista do Instituto de Medicina Tropical de São Paulo

versión impresa ISSN 0036-4665

Rev. Inst. Med. trop. S. Paulo vol.30 no.5 São Paulo sept./oct. 1988

http://dx.doi.org/10.1590/S0036-46651988000500003 

ORIGINAL ARTICLES

 

Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats

 

Paracoccidioidomicose: estudo histopatológico sequencial das lesões em ratos experimentalmente infectados

 

 

I.B. KerrI; J.R. AraripeI, III; P.C. OliveiraII; H.L. LenziI

IDepartment of Pathology of the Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ., Brazil
IIDepartment of Mycology of the Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ., Brazil
IIIRecipient of a CNPq Fellowship (Nº 822868-86.5)

Address for correspondence

 

 


SUMMARY

Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 106 cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium.
Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy.
The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous.
Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods.
Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time.

Key words: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Granuloma; Extracellular matrix; Collagen; Proteoglycans.


RESUMO

Foram utilizados ratos albinos, fêmeas, para o estudo histopatológico sequencial da paracoccidioidomicose experimental. Os animais39 foram inoculados intraperitonealmente com uma cepa de Paracoccidioides brasiliensis na fase leveduriforme e sacrificados, em determinados intervalos, a partir de 1 a 168 dias pós-infecção; cada animal recebeu um inóculo de 4 x 106 células em 0,8 ml de salina. Os animais controles receberam salina contendo raspado do meio de cultura.
Foram estudados tecidos correspondentesà área de inoculação. Analisou-se pela microscopia óptica o processo inflamatório granulomatoso em todo o seu conjunto, estudando a população celular, a matriz extracelular e a presença e características do fungo.
Os resultados possibilitaram desmembrar a cinética da resposta inflamatória em três fases: 1) neutrofílica ou macrofágica-neutrofílica; 2) pré-granulomatosa; 3) granulomatosa.
A síntese de matriz extracelular iniciou-se pela deposição de material fibrinóide, intensificando-se de modo gradativo com depósito de colágeno, de proteoglicanos e glicoproteínas. Os parasitos estavam presentes em todas as fases estudadas.
Períodos de reativação da doença eram nitidamente evidenciados através da concomitância de granulomas recém-formados com granulomas mais antigos, indicando que o processo granulomatoso neste modelo não resolve a doença, nem tão pouco consegue limitar a disseminação do fungo por um período prolongado.


 

 

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Full text available only in PDF format.

 

 

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Address for correspondence:
Itália B. Kerr
Departamento de Patologia
Instituto Oswaldo Cruz, FIOCRUZ
Caixa Postal 926. CEP 20010 Rio de Janeiro, RJ, Brasil

Recebido para publicação em 10/3/1988.