Immunopathology of human schistosomiasis mansoni: II. NK activity and stimulation by specific antigen

Benarroch, L. K.; Noya, O.; Noya, B.; Bianco, N. E.; Blanca, I.

doi:10.1590/S0036-46651988000600002

Abstracts

Sixteen S. mansoni infected and untreated patients (5 with recent infection and 11 with chronic disease) were evaluated for their in vitro natural killer (NK) activity against the NK sensitive target K562 cell line. NK levels in 9 out of 11 patients (82%) with chronic disease were significantly lower (mean = 15 ± 6%),compared with patients recently infected (mean = 41 ± 9% p < 0.001) and with the control group (mean = 38 ± 13% p < 0.001). However, both patients and controls NK activity was stimulated by soluble adult worm antigens (SAWA), indicating that NK function even in the chronic stage of the infection is able to respond to the parasite antigens. These results suggest the possibility of NK cell participation as effector mechanism against S. mansoni.

Natural Killer cells (NK); S. mansoni

Dezesseis doentes infectados e não tratados com S. mansoni (5 com infecção recente e 11 com doença crônica), foram submetidos à avaliação de atividade de células exterminadoras na turais (NK) "in vitro" frente a células alvo de linhagem K562. Os níveis de atividade das céluIas NK em 9 de 11 doentes (827r) com a infecção crônica foram significativamente menores (média = 15 ± 6%) quando comparados aos pacientes com infecção recente (média =41 ± 9%, p < 0,001) e aos indivíduos do grupo controle (média = 38 ± 13%, p < 0,001). Porém, tanto nos doentes como nos controles, a atividade de céluIas NK foi estimulada pelo antígeno solúvel do parasito adulto (SAWA), indicando que as céluIas NK, mesmo na fase crônica da infecção, têm capacidade de responder ao antígeno dos parasitos. Estes resultados sugerem a possível participação das células NK no mecanismo efetor de defesa contra o S. mansoni.

ORIGINAL ARTICLES

Immunopathology of human schistosomiasis mansoni. II. NK activity and stimulation by specific antigen

Imunopatologia da esquistossomose mansônica humana. II. Atividade NK e estimulação por antígeno específico

L. K. Benarroch; O. Noya; B. Noya; N. E. Bianco; I. Blanca

Clinical Immunology National Center, WHO PAHO. Collaborative Center for Clinical Immunology and the Tropical Medicine and Pathology Institutes, Central University. Venezuela

^{Address for correspondence} Address for correspondence: Isaac Blanca. MSc Inmunologia Clínica Apartado 50647 Correos de Sabana Grande Caracas 1051, Venezuela

SUMMARY

Sixteen S. mansoni infected and untreated patients (5 with recent infection and 11 with chronic disease) were evaluated for their in vitro natural killer (NK) activity against the NK sensitive target K562 cell line. NK levels in 9 out of 11 patients (82%) with chronic disease were significantly lower (mean = 15 ± 6%),compared with patients recently infected (mean = 41 ± 9% p < 0.001) and with the control group (mean = 38 ± 13% p < 0.001). However, both patients and controls NK activity was stimulated by soluble adult worm antigens (SAWA), indicating that NK function even in the chronic stage of the infection is able to respond to the parasite antigens. These results suggest the possibility of NK cell participation as effector mechanism against S. mansoni.

Key words: Natural Killer cells (NK); S. mansoni.

RESUMO

Dezesseis doentes infectados e não tratados com S. mansoni (5 com infecção recente e 11 com doença crônica), foram submetidos à avaliação de atividade de células exterminadoras na turais (NK) "in vitro" frente a células alvo de linhagem K562. Os níveis de atividade das céluIas NK em 9 de 11 doentes (827r) com a infecção crônica foram significativamente menores (média = 15 ± 6%) quando comparados aos pacientes com infecção recente (média =41 ± 9%, p < 0,001) e aos indivíduos do grupo controle (média = 38 ± 13%, p < 0,001). Porém, tanto nos doentes como nos controles, a atividade de céluIas NK foi estimulada pelo antígeno solúvel do parasito adulto (SAWA), indicando que as céluIas NK, mesmo na fase crônica da infecção, têm capacidade de responder ao antígeno dos parasitos. Estes resultados sugerem a possível participação das células NK no mecanismo efetor de defesa contra o S. mansoni.

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ACKNOWLEDGMENTS

Research supported by grants from CONICIT (Fortalecimiento de Centros) and from CDCH-M-09.15/84 (UCV).

We thank Amanda Gonzalez for editing the manuscript.

Recebido para publicação em 29 7 1987

1. ABE, T.; FORBES. T. J. & COLLEY, D. G. - Natural Killer cell activity during murine Schistosomiasis man soni. J. Parasit., 69: 1001-1005, 1983.
2. ATALLAH, A. M.; LEWIS, F. A.; URRITIA SHAW, A.; FOLKS, T. & YEATMAN, T. - Natural Killer cells (NK) and antibody dependent cells mediated cytotoxicity (ADCC) components of Schistosoma mansoni infection. Int. Arch. Allergy, 63: 351-354-, 1980.
3. BARSOUM, I. S.; FREMAN, J. R.; HABIB, M.; EL-ALA MY. M. A.; ROCHA, R. S.; KATZ, N.; GRAZZINELLI, G. & COLLEY, D. G. - Evaluation of Natural Killer activity in human Schistosomiasis. Amer. J. trop. Med. Hyg., 33: 451-454, 1984.
4. BOYUM, A. J. - Isolation of mononuclear cells and granulocytes from human blood. Scand. J. clin. Lab. Invest., 97: 1-10, 1968.
5. BRUNNER. K. T.; ENGERS, H. D. & CEROTTINI. J. C. - The ⁵¹Cr release assay as used the quantitative measure ment of cell mediated cytolysis in vitro In: BLOOM, B. R & DAVID, J R., ed. - in vitro methods in cell-mediated and tumor immunity. New York, Academic Press. 1976. p. 423-428.
6. COLLEY. D. G.; COOK, J. A.; FREEMAN. G. L.: BARTHOLOMEW, R. K. & JORDAN. P. - Immune responses during human Schistosomiasis mansoni. I. in vitro lymphocyte blastogenic response to heterogeneous antigenic preparations from Schistosoma eggs, worms and cercaria. Int. Arch. Allergy, 53: 420-433, 1977.
7. GASTL, G. A.; FELDMEIR, H.; DOEHING, E.; KORTMAN, C; DAFFALLA, A. A. & PETER, H. H. - Numerical and functional alteration of lymphocytes in human Schistosomiasis. Scand. J. Immunol., 19: 469-479, 1984.
8. HATOHER, F. M.; CERRONE, M. C. & BURTON, R. C. - Increased natural killer cells activity in experimental American trypanosomiasis. J. Immunol., 127: 1126-1130, 1981.
9. HAUSER, W. E.; SHARMA, S. D. & REMINGTON, J. S. - Natural Killer cells induced by acute and chronic toxoplasma infection. Cell. Immunol., 69: 330-346. 1982.
10. HAUSER, J. R. & TSAI, V. - Acute toxoplasma infection of mice induces spleen NK cells that are cytotoxic for T. gondii in vitro. J. Immunol., 136: 313-319, 1986
11. HERBERMAN, R. B. - Immune surveillance hypothesis. Update formulation and possible effector mechanisms. In: YAMAMURA, Y. & TADA, T., ed. - Fifth International Congress of Immunology. Orlando, Academic Press, 1983. p. 1157-1167.
12. JULIUS, M. H , SIMPSON, E. & HERZENBERG, L. A. - A rapid method for isolation of functional thymus derived murine lymphocytes. Europ. J. Immunol., 3: 645, 1973.
13. KAGAN, I. & PELLEGRINO, J. - A critical review of immunological methods for diagnosis of bilharziasis. Bull. Wld. Hlth. Org., 25: 611-674, 1961.
14. KATZ, N.; CHAVES, A. & PELLEGRINO, J. - A simple device for quantitative stool thick-smear technic in Schistosomiasis mansoni. Rev. Inst. Med. trop. S. Paulo, 14: 397-400, 1972.
15. LOPEZ, C. - Genetic resistance to herpes virus infections; role of natural killer cells. In: SHAMENE, S.; KONGSHAOW, P. A. L. & LANDY, M., ed. - Genetic control of natural resistance to infection and malignancy. New York, Academic Press, 1980. p. 253.
16. LOWRY, I. H.; ROSEMBLUM, O. H.; FARR, A. L. & RANDALL, R. J. - Protein measurement with the folin phenol reagent. J. biol. Chem., 193: 265-275, 1951.
17. OLIVER GONZALES, J. - Anti-egg precipitins in sera of human infected with Schistosoma mansoni, J. infect. Dis., 95: 86-91, 1954.
18. ORTALDO, J. R. & HERBERMAN, R. B. - Heterogeneity of natural killer cells. In: PAUL, W. E.; FATHMAN, C. G. & METZGER, H., ed. - Annual Reviews of Immunology, Palo Alto, California, Annual Reviews Inc., 1984. V. 2, p. 359-394.
19. TIMONEN, T.; ORTALDO, J. R. & HERBERMAN, R. B. - Characteristics of human large granular lymphocyte and relationship to natural killer and K cells J. exp. Med., 153: 569-582, 1981.
20. TRINCHIERI, G. & PERUSSIA, B. - Biology of disease. Human natural killer cells: biologic and pathologic as pects. Lab. Invest., 50: 489, 1984.
21. VOLLER, A.; BARTLETT, A. & BIDWELL, D. - Enzyme immunoassays in parasitic diseases. Trans. roy. Soc. trop. Med. Hyg., 70: 98-105, 1976.

Address for correspondence:

Isaac Blanca. MSc

Inmunologia Clínica

Apartado 50647

Correos de Sabana Grande

Caracas 1051, Venezuela

Publication Dates

Publication in this collection
17 Feb 2011
Date of issue
Dec 1988

History

Received
29 July 1987

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ABE, T.; FORBES. T. J. & COLLEY, D. G. - Natural Killer cell activity during murine Schistosomiasis man soni. J. Parasit., 69: 1001-1005, 1983.

[2] 2. ATALLAH, A. M.; LEWIS, F. A.; URRITIA SHAW, A.; FOLKS, T. & YEATMAN, T. - Natural Killer cells (NK) and antibody dependent cells mediated cytotoxicity (ADCC) components of Schistosoma mansoni infection. Int. Arch. Allergy, 63: 351-354-, 1980.

[3] 3. BARSOUM, I. S.; FREMAN, J. R.; HABIB, M.; EL-ALA MY. M. A.; ROCHA, R. S.; KATZ, N.; GRAZZINELLI, G. & COLLEY, D. G. - Evaluation of Natural Killer activity in human Schistosomiasis. Amer. J. trop. Med. Hyg., 33: 451-454, 1984.

[4] 4. BOYUM, A. J. - Isolation of mononuclear cells and granulocytes from human blood. Scand. J. clin. Lab. Invest., 97: 1-10, 1968.

[5] 5. BRUNNER. K. T.; ENGERS, H. D. & CEROTTINI. J. C. - The ⁵¹Cr release assay as used the quantitative measure ment of cell mediated cytolysis in vitro In: BLOOM, B. R & DAVID, J R., ed. - in vitro methods in cell-mediated and tumor immunity. New York, Academic Press. 1976. p. 423-428.

[6] 6. COLLEY. D. G.; COOK, J. A.; FREEMAN. G. L.: BARTHOLOMEW, R. K. & JORDAN. P. - Immune responses during human Schistosomiasis mansoni. I. in vitro lymphocyte blastogenic response to heterogeneous antigenic preparations from Schistosoma eggs, worms and cercaria. Int. Arch. Allergy, 53: 420-433, 1977.

[7] 7. GASTL, G. A.; FELDMEIR, H.; DOEHING, E.; KORTMAN, C; DAFFALLA, A. A. & PETER, H. H. - Numerical and functional alteration of lymphocytes in human Schistosomiasis. Scand. J. Immunol., 19: 469-479, 1984.

[8] 8. HATOHER, F. M.; CERRONE, M. C. & BURTON, R. C. - Increased natural killer cells activity in experimental American trypanosomiasis. J. Immunol., 127: 1126-1130, 1981.

[9] 9. HAUSER, W. E.; SHARMA, S. D. & REMINGTON, J. S. - Natural Killer cells induced by acute and chronic toxoplasma infection. Cell. Immunol., 69: 330-346. 1982.

[10] 10. HAUSER, J. R. & TSAI, V. - Acute toxoplasma infection of mice induces spleen NK cells that are cytotoxic for T. gondii in vitro. J. Immunol., 136: 313-319, 1986

[11] 11. HERBERMAN, R. B. - Immune surveillance hypothesis. Update formulation and possible effector mechanisms. In: YAMAMURA, Y. & TADA, T., ed. - Fifth International Congress of Immunology. Orlando, Academic Press, 1983. p. 1157-1167.

[12] 12. JULIUS, M. H , SIMPSON, E. & HERZENBERG, L. A. - A rapid method for isolation of functional thymus derived murine lymphocytes. Europ. J. Immunol., 3: 645, 1973.

[13] 13. KAGAN, I. & PELLEGRINO, J. - A critical review of immunological methods for diagnosis of bilharziasis. Bull. Wld. Hlth. Org., 25: 611-674, 1961.

[14] 14. KATZ, N.; CHAVES, A. & PELLEGRINO, J. - A simple device for quantitative stool thick-smear technic in Schistosomiasis mansoni. Rev. Inst. Med. trop. S. Paulo, 14: 397-400, 1972.

[15] 15. LOPEZ, C. - Genetic resistance to herpes virus infections; role of natural killer cells. In: SHAMENE, S.; KONGSHAOW, P. A. L. & LANDY, M., ed. - Genetic control of natural resistance to infection and malignancy. New York, Academic Press, 1980. p. 253.

[16] 16. LOWRY, I. H.; ROSEMBLUM, O. H.; FARR, A. L. & RANDALL, R. J. - Protein measurement with the folin phenol reagent. J. biol. Chem., 193: 265-275, 1951.

[17] 17. OLIVER GONZALES, J. - Anti-egg precipitins in sera of human infected with Schistosoma mansoni, J. infect. Dis., 95: 86-91, 1954.

[18] 18. ORTALDO, J. R. & HERBERMAN, R. B. - Heterogeneity of natural killer cells. In: PAUL, W. E.; FATHMAN, C. G. & METZGER, H., ed. - Annual Reviews of Immunology, Palo Alto, California, Annual Reviews Inc., 1984. V. 2, p. 359-394.

[19] 19. TIMONEN, T.; ORTALDO, J. R. & HERBERMAN, R. B. - Characteristics of human large granular lymphocyte and relationship to natural killer and K cells J. exp. Med., 153: 569-582, 1981.

[20] 20. TRINCHIERI, G. & PERUSSIA, B. - Biology of disease. Human natural killer cells: biologic and pathologic as pects. Lab. Invest., 50: 489, 1984.

[21] 21. VOLLER, A.; BARTLETT, A. & BIDWELL, D. - Enzyme immunoassays in parasitic diseases. Trans. roy. Soc. trop. Med. Hyg., 70: 98-105, 1976.

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Brasil

Immunopathology of human schistosomiasis mansoni: II. NK activity and stimulation by specific antigen

Imunopatologia da esquistossomose mansônica humana: II. Atividade NK e estimulação por antígeno específico

Abstracts

Publication Dates

History