Reduced schedule of human anti rabies immunization with Fuenzalida &amp; Palacios vaccine

Zanetti, Carlos Roberto; Favoretto, Silvana Regina; Tino, Milene Silva; Albas, Avelino; Valentini, Elizabeth Juliana G.; Pereira, Octávio Augusto de C.

doi:10.1590/S0036-46651989000100005

Abstracts

The present study evaluates the humoral and cellular immune responses in 35 volunteers submited to short antirabies vaccination schedules with the Fuenzalida & Palacios vaccine based on the administration of doses on non consecutive days. The volunteers were divided into two groups. The first group received a total number of five doses given on days 0, 4, 7, 20 and 35. The other group received four doses, the first one being a double dose given on day 0 and than three other single doses on days 7, 20 and 35. The evaluation of humoral immune response was carried out by serum neutralization (SN) and indirect immunofluorescense (IIF) tests, while the cellular immune response was evaluated by lymphoblastic transformation assay (LTA) and skin test (ST). According to our results these reduced schedules elicited early and effective humoral and cellulafimmune responses to rabies antigen suggesting that new reduced schedules should be extensively studied in order to give the proper bases to the proposition of changes in the current long-term schedule.

Antirabies vaccination; Reduced schedule; Cellular immunity

O objetivo do presente trabalho foi estudar um novo esquema de vacinação anti-rábica humana, com um menor número de doses, administradas em dias não consecutivos (5 doses nos dias 0, 4, 7, 20 e 35). A avaliação da resposta imune humoral foi feita pela prova de soroneutralização e pela reação de imunofluorescência indireta, enquanto que a resposta imune celular foi avaliada pela transformação linfoblástica em cultura de sangue total e pelo teste cutâneo de leitura tardia. Foram estudados um total de 35 voluntários, submetidos ao esquema reduzido de vacinação, e os resultados encontrados permitem afirmar que, embora o número de casos seja relativamente pequeno, este novo esquema de vacinação mostrou-se capaz de induzir a produção de imunoglobulinas anti-rábicas, bem como de eli-citar a resposta imune celular ao antígeno rábico.

ORIGINAL ARTICLES

Reduced schedule of human anti rabies immunization with Fuenzalida & Palacios vaccine

Esquema reduzido de vacinação anti-rábica humana

Carlos Roberto Zanetti^I; Silvana Regina Favoretto^I; Milene Silva Tino^II; Avelino Albas^III; Elizabeth Juliana G. Valentini^III; Octávio Augusto de C. Pereira^II

^IInstituto Pasteur de São Paulo, São Paulo, SP., Brasil

^IIDisciplina de Imunologia da Escola Paulista de Medicina, São Paulo, SP., Brasil

^IIIInstituto Butantan, São Paulo, SP., Brasil

^{Address for correspondence} Address for correspondence: Dr. Carlos Roberto Zanetti Instituto Pasteur de São Paulo Avenida Paulista, 393 CEP 01311, São Paulo, SP., Brasil

SUMMARY

The present study evaluates the humoral and cellular immune responses in 35 volunteers submited to short antirabies vaccination schedules with the Fuenzalida & Palacios vaccine based on the administration of doses on non consecutive days.

The volunteers were divided into two groups. The first group received a total number of five doses given on days 0, 4, 7, 20 and 35. The other group received four doses, the first one being a double dose given on day 0 and than three other single doses on days 7, 20 and 35.

The evaluation of humoral immune response was carried out by serum neutralization (SN) and indirect immunofluorescense (IIF) tests, while the cellular immune response was evaluated by lymphoblastic transformation assay (LTA) and skin test (ST).

According to our results these reduced schedules elicited early and effective humoral and cellulafimmune responses to rabies antigen suggesting that new reduced schedules should be extensively studied in order to give the proper bases to the proposition of changes in the current long-term schedule.

Key words: Antirabies vaccination; Reduced schedule; Cellular immunity.

RESUMO

O objetivo do presente trabalho foi estudar um novo esquema de vacinação anti-rábica humana, com um menor número de doses, administradas em dias não consecutivos (5 doses nos dias 0, 4, 7, 20 e 35).

A avaliação da resposta imune humoral foi feita pela prova de soroneutralização e pela reação de imunofluorescência indireta, enquanto que a resposta imune celular foi avaliada pela transformação linfoblástica em cultura de sangue total e pelo teste cutâneo de leitura tardia.

Foram estudados um total de 35 voluntários, submetidos ao esquema reduzido de vacinação, e os resultados encontrados permitem afirmar que, embora o número de casos seja relativamente pequeno, este novo esquema de vacinação mostrou-se capaz de induzir a produção de imunoglobulinas anti-rábicas, bem como de eli-citar a resposta imune celular ao antígeno rábico.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

ACKNOWLEDGEMENTS

The authors wish to thank all volunteers who took part in this work. This work was supported by CNPq, CAPES and FINEP.

Recebido para publicação em 5/8/1988/

1. ANDRADE, P. V. - Aspectos da profilaxia da raiva no Estado de São Paulo. Arch. Hig. (S. Paulo), 26: 217-218, 1961.
2. ATANASIU, P. - Titrage des anticorps rabiques pratique sur les sérum humains. Bull. Off. Int. Epizoot., 67 (34): 383_;387, 1967.
3. FUENZALIDA, E. & PALACIOS, R. - Un método mejorado en la preparación de la vacuna antirrábica. Bol. Inst. bact. Chile, 8: 3-10, 1955.
4. GOLDWASSER, R. A. & KISSLING, R. E. - Fluorescent antibody staining of street and fixed rabies virus antigens. Proc. Soc. exp. Biol. (N. Y.), 98: 219-223, 1958.
5. HALL, L. S. & GORDON, D. S. - Reproducibility, efficacy and methodology of mitogen-induced lymphocyte transformations by the whole blood assay. J. Immunol. Meth., 12: 31-38, 1976.
6. JOHNSON, S. E. & PEARSON, E. W. - Clinical responses in humans to rabies vaccine prepared in MRC-5 diploid cells from Canadian seed virus. In: KUWERT, E.; MERIEUX, C; KOPROWSKI, H.; BOGEL, K., ed. - Rabies in the tropics Berlin, Springer-Verlag, 1985. p. 99-105.
7. MIFUNE, K.; TAKEUCHI, E.; NAPIORKOWSKI, P. A.; YAMADA, A. & SAKAMOTO, K. - Essential role of T cells in the postexposure prophylaxis of rabies in mjce. Microbiol. Immunol., 25: 895-904, 1981.
8. TURNER, G. S. - Thymus dependence of rabies vaccine. J. gen. Virol., 33: 535-538, 1976.
9. VEIGA, D. R.; ZANETTI, C. R.; MENDES, N. F.; PEREIRA, O. A. C. - Cell mediated immune response in human antirabies revaccination. Rev. Inst. Med. trop. S. Paulo, 29: 104-109, 1987.
10. WIKTOR, T. J. - Cell mediated immunity and postexposure protection from rabies by inactivated vaccines of tissue culture origin. Develop. biol. Standard., 40: 255-264, 1978.

Address for correspondence:

Dr. Carlos Roberto Zanetti

Instituto Pasteur de São Paulo

Avenida Paulista, 393

CEP 01311, São Paulo, SP., Brasil

Publication Dates

Publication in this collection
18 Feb 2011
Date of issue
Feb 1989

History

Received
05 Aug 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ANDRADE, P. V. - Aspectos da profilaxia da raiva no Estado de São Paulo. Arch. Hig. (S. Paulo), 26: 217-218, 1961.

[2] 2. ATANASIU, P. - Titrage des anticorps rabiques pratique sur les sérum humains. Bull. Off. Int. Epizoot., 67 (34): 383_;387, 1967.

[3] 3. FUENZALIDA, E. & PALACIOS, R. - Un método mejorado en la preparación de la vacuna antirrábica. Bol. Inst. bact. Chile, 8: 3-10, 1955.

[4] 4. GOLDWASSER, R. A. & KISSLING, R. E. - Fluorescent antibody staining of street and fixed rabies virus antigens. Proc. Soc. exp. Biol. (N. Y.), 98: 219-223, 1958.

[5] 5. HALL, L. S. & GORDON, D. S. - Reproducibility, efficacy and methodology of mitogen-induced lymphocyte transformations by the whole blood assay. J. Immunol. Meth., 12: 31-38, 1976.

[6] 6. JOHNSON, S. E. & PEARSON, E. W. - Clinical responses in humans to rabies vaccine prepared in MRC-5 diploid cells from Canadian seed virus. In: KUWERT, E.; MERIEUX, C; KOPROWSKI, H.; BOGEL, K., ed. - Rabies in the tropics Berlin, Springer-Verlag, 1985. p. 99-105.

[7] 7. MIFUNE, K.; TAKEUCHI, E.; NAPIORKOWSKI, P. A.; YAMADA, A. & SAKAMOTO, K. - Essential role of T cells in the postexposure prophylaxis of rabies in mjce. Microbiol. Immunol., 25: 895-904, 1981.

[8] 8. TURNER, G. S. - Thymus dependence of rabies vaccine. J. gen. Virol., 33: 535-538, 1976.

[9] 9. VEIGA, D. R.; ZANETTI, C. R.; MENDES, N. F.; PEREIRA, O. A. C. - Cell mediated immune response in human antirabies revaccination. Rev. Inst. Med. trop. S. Paulo, 29: 104-109, 1987.

[10] 10. WIKTOR, T. J. - Cell mediated immunity and postexposure protection from rabies by inactivated vaccines of tissue culture origin. Develop. biol. Standard., 40: 255-264, 1978.