SciELO - Scientific Electronic Library Online

vol.31 issue1Inapparent lung involvement in patients with the subacute juvenile type of paracoccidioidomycosisRecent occurence of human infection by Rocio arbovirus in Ribeira Valley, Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.31 no.1 São Paulo Jan./Feb. 1989 



Reduced schedule of human anti rabies immunization with Fuenzalida & Palacios vaccine


Esquema reduzido de vacinação anti-rábica humana



Carlos Roberto ZanettiI; Silvana Regina FavorettoI; Milene Silva TinoII; Avelino AlbasIII; Elizabeth Juliana G. ValentiniIII; Octávio Augusto de C. PereiraII

IInstituto Pasteur de São Paulo, São Paulo, SP., Brasil
IIDisciplina de Imunologia da Escola Paulista de Medicina, São Paulo, SP., Brasil
IIIInstituto Butantan, São Paulo, SP., Brasil

Address for correspondence




The present study evaluates the humoral and cellular immune responses in 35 volunteers submited to short antirabies vaccination schedules with the Fuenzalida & Palacios vaccine based on the administration of doses on non consecutive days.
The volunteers were divided into two groups. The first group received a total number of five doses given on days 0, 4, 7, 20 and 35. The other group received four doses, the first one being a double dose given on day 0 and than three other single doses on days 7, 20 and 35.
The evaluation of humoral immune response was carried out by serum neutralization (SN) and indirect immunofluorescense (IIF) tests, while the cellular immune response was evaluated by lymphoblastic transformation assay (LTA) and skin test (ST).
According to our results these reduced schedules elicited early and effective humoral and cellulafimmune responses to rabies antigen suggesting that new reduced schedules should be extensively studied in order to give the proper bases to the proposition of changes in the current long-term schedule.

Key words: Antirabies vaccination; Reduced schedule; Cellular immunity.


O objetivo do presente trabalho foi estudar um novo esquema de vacinação anti-rábica humana, com um menor número de doses, administradas em dias não consecutivos (5 doses nos dias 0, 4, 7, 20 e 35).
A avaliação da resposta imune humoral foi feita pela prova de soroneutralização e pela reação de imunofluorescência indireta, enquanto que a resposta imune celular foi avaliada pela transformação linfoblástica em cultura de sangue total e pelo teste cutâneo de leitura tardia.
Foram estudados um total de 35 voluntários, submetidos ao esquema reduzido de vacinação, e os resultados encontrados permitem afirmar que, embora o número de casos seja relativamente pequeno, este novo esquema de vacinação mostrou-se capaz de induzir a produção de imunoglobulinas anti-rábicas, bem como de eli-citar a resposta imune celular ao antígeno rábico.



Texto completo disponível apenas em PDF.

Full text available only in PDF format.




The authors wish to thank all volunteers who took part in this work. This work was supported by CNPq, CAPES and FINEP.



1. ANDRADE, P. V. - Aspectos da profilaxia da raiva no Estado de São Paulo. Arch. Hig. (S. Paulo), 26: 217-218, 1961.         [ Links ]

2. ATANASIU, P. - Titrage des anticorps rabiques pratique sur les sérum humains. Bull. Off. Int. Epizoot., 67 (34): 383;387, 1967.         [ Links ]

3. FUENZALIDA, E. & PALACIOS, R. - Un método mejorado en la preparación de la vacuna antirrábica. Bol. Inst. bact. Chile, 8: 3-10, 1955.         [ Links ]

4. GOLDWASSER, R. A. & KISSLING, R. E. - Fluorescent antibody staining of street and fixed rabies virus antigens. Proc. Soc. exp. Biol. (N. Y.), 98: 219-223, 1958.         [ Links ]

5. HALL, L. S. & GORDON, D. S. - Reproducibility, efficacy and methodology of mitogen-induced lymphocyte transformations by the whole blood assay. J. Immunol. Meth., 12: 31-38, 1976.         [ Links ]

6. JOHNSON, S. E. & PEARSON, E. W. - Clinical responses in humans to rabies vaccine prepared in MRC-5 diploid cells from Canadian seed virus. In: KUWERT, E.; MERIEUX, C; KOPROWSKI, H.; BOGEL, K., ed. - Rabies in the tropics. Berlin, Springer-Verlag, 1985. p. 99-105.         [ Links ]

7. MIFUNE, K.; TAKEUCHI, E.; NAPIORKOWSKI, P. A.; YAMADA, A. & SAKAMOTO, K. - Essential role of T cells in the postexposure prophylaxis of rabies in mjce. Microbiol. Immunol., 25: 895-904, 1981.         [ Links ]

8. TURNER, G. S. - Thymus dependence of rabies vaccine. J. gen. Virol., 33: 535-538, 1976.         [ Links ]

9. VEIGA, D. R.; ZANETTI, C. R.; MENDES, N. F.; PEREIRA, O. A. C. - Cell mediated immune response in human antirabies revaccination. Rev. Inst. Med. trop. S. Paulo, 29: 104-109, 1987.         [ Links ]

10. WIKTOR, T. J. - Cell mediated immunity and postexposure protection from rabies by inactivated vaccines of tissue culture origin. Develop. biol. Standard., 40: 255-264, 1978.         [ Links ]



Address for correspondence:
Dr. Carlos Roberto Zanetti
Instituto Pasteur de São Paulo
Avenida Paulista, 393
CEP 01311, São Paulo, SP., Brasil

Recebido para publicação em 5/8/1988/

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License