SciELO - Scientific Electronic Library Online

 
vol.31 issue2Active immunization against hepatitis B virus (HBV) with low-doses of plasma-derived vaccine by intradermal routeUsefulness of dry blood samples on filter paper in the detection of antitoxoplasma antibodies author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.31 no.2 São Paulo Mar./Apr. 1989

http://dx.doi.org/10.1590/S0036-46651989000200006 

ARTIGOS ORIGINAIS

 

Estudo sorológico de infecções experimentais por Trypanosoma evansi, em cobaias

 

Studies on the serological diagnosis of experimental infection with Trypanosoma evansi in guinea-pigs

 

 

Teresa Cristina Goulart de OliveiraI; Roberto SogayarII; Ednir SalataII

IProfa Assistente - Deptº de Parasitologia - IB/UNESP, Campus de Botucatu. CEP 18610 Botucatu, SP, Brasil
IIProf. Assistente Doutor - Deptº de Parasitologia - IB/UNESP, Campus de Botucatu. Botucatu, SP, Brasil

 

 


RESUMO

Comparamos os métodos de Imunofluorescência Indireta (IFI), Imunodifusão Radial Dupla e Aglutinação, para a pesquisa de anticorpos em soros, na tripanossomíase experimental por Trypanosoma evansi, em cobaias. Foram obtidas 20 amostras de soro correspondentes às 4 primeiras semanas de infecção. A IFI foi positiva em apenas 6 animais, com títulos variando de 1:4 a 1:16. Os títulos mais altos foram observados na 3a semana pós-infecção. Anticorpos aglutinantes foram observados a partir da 1a semana pós-infecção e, após a 2a semana, todos os animais apresentaram reação de aglutinação positiva, com títulos variando de 1:8.000 a 1:250.000. O tratamento dos soros com 2-Mercapto-etanol inibiu a reação de aglutinação, sugerindo ser IgM a principal classe dos anticorpos presentes no soro dos animais infectados. Não se constatou a presença de anticorpos precipitantes durante todo o curso da infecção.

Unitermos: Trypanosoma evansi; Sorologia; Infecção experimental.


SUMMARY

Sera of 20 guinea-pigs experimentally infected with Trypanosoma evansi were obtained in order to compare the efficacy of gel diffusion, indirect immunofluorescence and agglutination tests, to detect antibodies to T. evansi. The fluorescent antibody test was positive in six (6) animals and the antibody titres were very low (1:4 to 1:16). The agglutination test detected trypanosomal antibodies in sera one (1) week after infection. After two (2) weeks all animals were positive with high titres (1:8.000 to 1:250.000). Agglutination was inhibited when sera were treated with 2-Mercapto-etanol. This fact suggests that IgM is the principal class of antibodies in sera of infected guinea-pigs. Precipitating antibodies were not detected during the course of infection.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

REFERENCIAS BIBLIOGRÁFICAS

1. ALLSOPP, B. A.; MJOGU, A. R. & HUMPHRYES, K. C. - Nature and location of Trypanosoma brucei subgroup exoantigen and its relationship to 4S antigen. Exp. Parasit., 29: 271-284, 1971.         [ Links ]

2. BOEHRINGER, E. G. - La doble difusión en agar en el diagnostico del "Mal de Caderas" (T. equinum). Rev. Med. vet. (B. Aires), 46: 425-431, 1965.         [ Links ]

3. CAMARGO, M. E.; FERREIRA, A. W.; ROCCA, A. & BELEM, Z. R. - Um teste prático para a sorologia da toxoplasmose: o teste de hemaglutinacáo. Estudo comparativo com os testes de imunofluorescéncia e imunoenzimático de captura de IgM. Rev. bras. Pat. clin., 22: 196-201. 1986.         [ Links ]

4. GILL, B. S. - A procedure for the indirect haemagglutination test for the study of experimentally Trypanosoma evansi infections. Ann. trop. Med. Parasit., 58: 473-480, 1964.         [ Links ]

5. GILL, B. S. - Studies on the serological diagnosis of Trypanosoma evansi. J. comp. Path., 75: 175-183, 1965.         [ Links ]

6. JATKAR, P. R.; RAO, P. V. & SINGH, M. - Diagnosis of Surra: capillary agglutination test. Indian vet. J., 54: 795-797, 1977.         [ Links ]

7. KLEIN, F.; MATTERN, P.; RADEMA, H. & VANZEWT, T. L. - Slowly sedimenting serum components reacting with antiIgM sera. Immunology, 13: 641-647, 1967.         [ Links ]

8. LOSOS, G. J. - Diseases caused by Trypanosoma evansi. A review. Vet. Res. Comm., 4: 165-181, 1980.         [ Links ]

9. LUCKINS, A. G.; GRAY, A. R. & RAE, P. - Comparison of the diagnostic value of serum immunoglobulin levels, an enzime-linked immunosorbent assay and a fluorescent antibody test in experimental infections with Trypanosoma evansi in rabbits. Ann. trop. Med. Parasit., 72: 429-441, 1978.         [ Links ]

10. LUCKINS, A. G.; BOID, R.; RAE, P.; MAHMOUD, M. M.; EL MAZIK, K. H. & GRAY, A. R. - Serodiagnosis of infection with Trypanosoma evansi in camels in the Sudan. Trop. Anim. Hlth. Prod., 11: 1-12, 1979.         [ Links ]

11. OLIVEIRA, T. C. G.; MENEGUIN, J. M. & PEREIRA, E. A. - Comportamento do Trypanosoma evansi (= T. equinum) em animáis de laboratorio. Arq. bras. Med. vet. Zoct., (No prelo).         [ Links ]

12. OUCHTERLONY, O. - Diffusion-in-gel methods for immunological analysis. Progr. Allergy, 5: 1, 1958.         [ Links ]

13. RAADT, P. de - Immunity and antigenic variation: clinical observations suggestive of immune phenomena in African trypanosomiasis. In: TRYPANOSOMIASIS AND LEISHMANIASIS WITH SPECIAL REFERENCE TO CHAGAS' DISEASE. Amsterdam, Elsevier, 1974. p. 199-216. (Ciba Foundation Symposium 20, new series).         [ Links ]

14. SEN, H. G.; DUTTA, B. N. & RAY, H. N. - Biochemical changes in experimentally induced Trypanosoma evansi infection in rabbits and guinea-pigs. Indian J. vet. Sci., 29: 118-123, 1959.         [ Links ]

15. VERMA, B. B. & GAUTAM, O. P. - Serological diagnosis of experimental bovine surra (Trypanosoma evansi) infection. A comparison of passive haemagglutination, gel diffusion and indirect fluorescent antibody tests. Indian vet. J., 59: 809-813, 1977.         [ Links ]

 

 

Recebido para publicação em 4/7/1988

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License