Differences in the antigenic profile of bloodstream and cell culture derived trypomastigotes of Trypanosoma cruzi

Silva, Ana M. M. Da; Brodskyn, Cláudia I.; Takehara, Harumi A.; Mota, I.

doi:10.1590/S0036-46651989000300003

Abstracts

A comparative study of the antigenic profile of bloodstream and cell culture derived trypomastigotes showed many differences in their components. Using mouse anti-T. cruzi antibodies the differences were located mostly in the 120 kDa band, whereas using chagasic patient sera the differences were located in the 85 and 52 kDa bands. These findings might explain known physiological differences between trypomatigotes obtained from cell culture and from infected blood. A brief report of this work has already been published9.

Trypanosoma cruzi; T. cruzi antigens; Trypomastigotes

O estudo comparativo do perfil antigênico de tripomastigotas sangüícolas e tripomatigotas obtidos por cultura celular do Trypanosoma cruzi revelou que estas formas apresentam diferenças em alguns de seus componentes. Utilizando-se anticorpos provenientes da infecção murina, as diferenças foram detectadas na região de 120 kDa enquanto soros de pacientes chagásicos detectaram diferenças nas regiões de 85 e 52 kDa. Estas observações podem oferecer uma explicação a diferenças fisiológicas que ocorrem entre os tripomastigotas sangüícolas e os obtidos de cultura celular.

ORIGINAL ARTICLES

Differences in the antigenic profile of bloodstream and cell culture derived trypomastigotes of Trypanosoma cruzi

Diferenças no perfil antigênico de tripomastigotas sangüícolas e de cultura celular do Trypanosoma cruzi

Ana M. M. Da Silva; Cláudia I. Brodskyn; Harumi A. Takehara; I. Mota

Centro de Pesquisa e Formação em Imunologia da OMS/OPS. Instituto Butantan, São Paulo, Brasil

^{Address for correspondence} Address for correspondence: Dra. Ana Maria Moura da Silva Centro de Pesquisa e Formação em Imunologia da OMS/OPS Instituto Butantan Av. Dr. Vital Brasil, 1500. Caixa Postal 65 CEP 05504 São Paulo, SP, Brasil

SUMMARY

A comparative study of the antigenic profile of bloodstream and cell culture derived trypomastigotes showed many differences in their components. Using mouse anti-T. cruzi antibodies the differences were located mostly in the 120 kDa band, whereas using chagasic patient sera the differences were located in the 85 and 52 kDa bands. These findings might explain known physiological differences between trypomatigotes obtained from cell culture and from infected blood. A brief report of this work has already been published⁹.

Key words:Trypanosoma cruzi; T. cruzi antigens; Trypomastigotes.

RESUMO

O estudo comparativo do perfil antigênico de tripomastigotas sangüícolas e tripomatigotas obtidos por cultura celular do Trypanosoma cruzi revelou que estas formas apresentam diferenças em alguns de seus componentes. Utilizando-se anticorpos provenientes da infecção murina, as diferenças foram detectadas na região de 120 kDa enquanto soros de pacientes chagásicos detectaram diferenças nas regiões de 85 e 52 kDa. Estas observações podem oferecer uma explicação a diferenças fisiológicas que ocorrem entre os tripomastigotas sangüícolas e os obtidos de cultura celular.

Full text available only in PDF format.

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ACKNOWLEDGMENTS

This work was supported by Conselho Nacional de Pesquisas, Brasil (Process 40.0695/85). We thank Mr. Carlos Jared for technical assistance and Dr. Rodolfo Pereira Mendes for kindly providing the sera of Chagas' disease patients.

Recebido para publicação em 2/11/1988.

1. ALVES, M. J. M.; ABUIM, G.; KUMAJIMA, V. Y. & COLLI, W. - Partial inhibition of trypomastigote entry into cultured mammalian cells by monoclonal antibodies against a surface glycoprotein of Trypanosoma cruzi Molec. Biochem. Parasit., 21: 75-82, 1986.
2. HOSHINO-SHIMIZU, S.,; CAMARGO, M. E.; SHIMIZU, T. & NAGASSE, T. K. - A study on the reproductibility of a stable, lyophilised reagent for the Chagas' disease haemagglutination test: proposals for quality control analysis. Rev. Inst. Med. trop. S. Paulo, 24: 63-68, 1982.
3. KANBARA, H. & NAKABAYASHI, T. - Comparative studies on trypomastigotes of Trypanosoma cruzi from infected mouse blood and infected fibroblast cell (L-cell) culture. Biken J., 26: 57-62, 1983.
4.KANBARA, H. & NAKABAYASHI, T. - Comparative studies on surface antigenicity of Trypanosoma cruzi trypomastigotes from infected mouse blood and infected L-cell. Biken J., 28: 71-77, 1985.
5. LAEMMLI, U. K. - Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685, 1970.
6. LANAR, D. E. & MANNING, J. E. - Major surface proteins and antigens on the different "in vivo" and "in vitro" forms of Trypanosoma cruzi Mol. Biochem. Parasit., 11: 119-131, 1984.
7. MEIRELLES, M. N. L.; CHIARI, E. & SOUZA, W. - Interaction of bloodstream tissue culture derived and axenic culture derived trypomastigotes of Trypanosoma cruzi with macrophages. Acta trop. (Basel), 39: 195-203, 1982.
8. SILVA, A. M. M.; COSTA, H. H.; TAKEHARA, H. A. & MOTA, I. - Trypanosoma cruzi: advantages of isolating bloodstream trypomastigotes by the carboxymethyl cellulose method. Trans, roy. Soc. trop. Med. Hyg., 82: 715-718, 1988.
9. SILVA, A. M. M.; BRODSKYN, C. I.; TAKEHARA, H. A. & MOTA, I. - Comparison between the antigenic composition of bloodstream and cell culture-derived trypomastigotes of Trypanosoma cruzi. Braz. J. med. biol. Res., 21: 991-993, 1988.
10. SILVA, L. H. P. & NUSSENZWEIG, V. - Sobre uma cepa de Trypanosoma cruzi altamente virulenta para camundongo branco. Folia clin. biol., 20: 191-207, 1953.
11. TOWBIN, H.; STAEHELIN, T. & GORDON, J. - Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. nat. Acad. Sei. (Wash.), 76: 4350 4354, 1979.

Address for correspondence:

Dra. Ana Maria Moura da Silva

Centro de Pesquisa e Formação em Imunologia da OMS/OPS

Instituto Butantan

Av. Dr. Vital Brasil, 1500. Caixa Postal 65

CEP 05504 São Paulo, SP, Brasil

Publication Dates

Publication in this collection
21 Feb 2011
Date of issue
June 1989

History

Received
02 Nov 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALVES, M. J. M.; ABUIM, G.; KUMAJIMA, V. Y. & COLLI, W. - Partial inhibition of trypomastigote entry into cultured mammalian cells by monoclonal antibodies against a surface glycoprotein of Trypanosoma cruzi Molec. Biochem. Parasit., 21: 75-82, 1986.

[2] 2. HOSHINO-SHIMIZU, S.,; CAMARGO, M. E.; SHIMIZU, T. & NAGASSE, T. K. - A study on the reproductibility of a stable, lyophilised reagent for the Chagas' disease haemagglutination test: proposals for quality control analysis. Rev. Inst. Med. trop. S. Paulo, 24: 63-68, 1982.

[3] 3. KANBARA, H. & NAKABAYASHI, T. - Comparative studies on trypomastigotes of Trypanosoma cruzi from infected mouse blood and infected fibroblast cell (L-cell) culture. Biken J., 26: 57-62, 1983.

[4] 4.KANBARA, H. & NAKABAYASHI, T. - Comparative studies on surface antigenicity of Trypanosoma cruzi trypomastigotes from infected mouse blood and infected L-cell. Biken J., 28: 71-77, 1985.

[5] 5. LAEMMLI, U. K. - Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685, 1970.

[6] 6. LANAR, D. E. & MANNING, J. E. - Major surface proteins and antigens on the different "in vivo" and "in vitro" forms of Trypanosoma cruzi Mol. Biochem. Parasit., 11: 119-131, 1984.

[7] 7. MEIRELLES, M. N. L.; CHIARI, E. & SOUZA, W. - Interaction of bloodstream tissue culture derived and axenic culture derived trypomastigotes of Trypanosoma cruzi with macrophages. Acta trop. (Basel), 39: 195-203, 1982.

[8] 8. SILVA, A. M. M.; COSTA, H. H.; TAKEHARA, H. A. & MOTA, I. - Trypanosoma cruzi: advantages of isolating bloodstream trypomastigotes by the carboxymethyl cellulose method. Trans, roy. Soc. trop. Med. Hyg., 82: 715-718, 1988.

[9] 9. SILVA, A. M. M.; BRODSKYN, C. I.; TAKEHARA, H. A. & MOTA, I. - Comparison between the antigenic composition of bloodstream and cell culture-derived trypomastigotes of Trypanosoma cruzi. Braz. J. med. biol. Res., 21: 991-993, 1988.

[10] 10. SILVA, L. H. P. & NUSSENZWEIG, V. - Sobre uma cepa de Trypanosoma cruzi altamente virulenta para camundongo branco. Folia clin. biol., 20: 191-207, 1953.

[11] 11. TOWBIN, H.; STAEHELIN, T. & GORDON, J. - Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. nat. Acad. Sei. (Wash.), 76: 4350 4354, 1979.

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