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Original Articles • Rev. Inst. Med. trop. S. Paulo 31 (4) • Aug 1989 • https://doi.org/10.1590/S0036-46651989000400001   copy

Interaction of rheumatoid factor and Entamoeba histolytica

Interação do fator reumatóide e Entamoeba histolytica

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstracts

The amoebae's cytotoxicity test and the amoebae's lysis test were used to show possible interactions between rheumatoid factor (RF) and Entamoeba histolytica. Amoebae's cytotoxic activity (ACA) was inhibited by affinity chromatography purified antiamoebae rabbit IgG (RIgG). Enhanced inhibition could be demonstrated with RIgG plus RF. But the same marked inhibition of ACA could be seen when replacing RF by heat inactivated normal human serum as a control. About 50% amoebae's lysis occurred when amoebae were brought together with native normal human serum (NNHS) as a source of complement. Amoebae's lysis increased to 60% when incubated with NHS plus human antiamoebae antibodies. No further augmentation could be obtained by the addition of RF. Using RIgG instead of human antibodies the lysis rate did not increase. Incubation of amoebae, NNHS, RIgG and RF even reduced amoebae's lysis. RF neither has an effect on ACA nor on complement mediated AL in vitro.

Entamoeba histolytica; Amoebae's lysis; Rheumatoid factor

Testes para citotoxicidade e lise amebiana foram utilizados para demonstrar uma possível interação entre o fator reumatóide e a Entamoeba histolytica. A atividade citotóxica amebiana foi inibida pela IgG antiameba de coelho purificada através de cromatografia. Constatou-se inibição aumentada com IgG antiameba de coelho mais fator reumatóide. A mesma inibição acentuada da atividade citotóxica amebiana pôde ser constatada quando se substituiu o fator reumatóide por soro humano normal, inativado pelo calor, como controle. Cerca de 50% de lise amebiana ocorreu quando as amebas foram misturadas com soro normal humano como fonte de complemento. A lise amebiana aumentou para 60% quando incubadas com soro humano normal, acrescido de anticorpos humanos antiameba. Nenhum aumento adicional pode ser obtido pela adição de fator reumatóide. Usando IgG antiameba de coelho em vez de anticorpos humanos, a proporção de lise não aumentou. A incubação de amebas com soro humano normal, IgG antiameba de coelho e fator reumatóide reduziu acentuadamente a lise amebiana. O fator reumatóide não teve efeito na atividade citotóxica amebiana, nem na lise amebiana mediada pelo complemento in vitro.

ORIGINAL ARTICLES

Interaction of rheumatoid factor and Entamoeba histolytica

Interação do fator reumatóide e Entamoeba histolytica

P.G. KremsnerI; W. GraningerII

IInstitute of Specific Prophylaxis and Tropical Medicine, Berlin

IIDepartment of Chemotherapy, University of Vienna, Austria

Address for correspondence Address for correspondence: Dr. P.G. Kremsner State Institute of Tropical Medicine Berlin Königin-Elisabeth-StraBe 32 - Berlin 19, FRG

SUMMARY

The amoebae's cytotoxicity test and the amoebae's lysis test were used to show possible interactions between rheumatoid factor (RF) and Entamoeba histolytica. Amoebae's cytotoxic activity (ACA) was inhibited by affinity chromatography purified antiamoebae rabbit IgG (RIgG). Enhanced inhibition could be demonstrated with RIgG plus RF. But the same marked inhibition of ACA could be seen when replacing RF by heat inactivated normal human serum as a control.

About 50% amoebae's lysis occurred when amoebae were brought together with native normal human serum (NNHS) as a source of complement. Amoebae's lysis increased to 60% when incubated with NHS plus human antiamoebae antibodies. No further augmentation could be obtained by the addition of RF. Using RIgG instead of human antibodies the lysis rate did not increase. Incubation of amoebae, NNHS, RIgG and RF even reduced amoebae's lysis. RF neither has an effect on ACA nor on complement mediated AL in vitro.

Key words:Entamoeba histolytica; Amoebae's lysis; Rheumatoid factor.

RESUMO

Testes para citotoxicidade e lise amebiana foram utilizados para demonstrar uma possível interação entre o fator reumatóide e a Entamoeba histolytica. A atividade citotóxica amebiana foi inibida pela IgG antiameba de coelho purificada através de cromatografia. Constatou-se inibição aumentada com IgG antiameba de coelho mais fator reumatóide. A mesma inibição acentuada da atividade citotóxica amebiana pôde ser constatada quando se substituiu o fator reumatóide por soro humano normal, inativado pelo calor, como controle. Cerca de 50% de lise amebiana ocorreu quando as amebas foram misturadas com soro normal humano como fonte de complemento. A lise amebiana aumentou para 60% quando incubadas com soro humano normal, acrescido de anticorpos humanos antiameba. Nenhum aumento adicional pode ser obtido pela adição de fator reumatóide. Usando IgG antiameba de coelho em vez de anticorpos humanos, a proporção de lise não aumentou. A incubação de amebas com soro humano normal, IgG antiameba de coelho e fator reumatóide reduziu acentuadamente a lise amebiana. O fator reumatóide não teve efeito na atividade citotóxica amebiana, nem na lise amebiana mediada pelo complemento in vitro.

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REFÊNCIAS BIBLIOGRÁFICAS

1. BOS, H.J. - Entamoeba histolytica: cytopathogenicity of intact amoebae and cell free extracts; isolation and characterization of an intracellular toxin. Exp. Parasit., 47:369-377, 1979.

2. CARSON, D.A.; BAYER, A.S.; EISENBERG, R.A.; LAW RANCE, S. & THEOFILOPOULOS, A. - IgG rheumatoid factor in subacute bacterial endocarditis: relationship to IgM rheumatoid factor and circulating immune complexes. Clin. exp. Immunol., 31:100-103, 1978.

3. CARVALHO, E.M.; ANDREWS, B.S.; MARTINELLI, R.; DUTRA, M. & ROCHA, H. - Circulating immune complexes and rheumatoid factor in schistosomiasis and visceral leishmaniasis. Amer. J. trop. Med. Hyg., 32:61-68, 1983.

4. CLARKSON, A.B. & MELLOW , G.H. - Rheumatoid factor - like immunoglobulin M protects preciously uninfected rat pups and dams from Trypanosoma lewi.si. Science, 214:186-188, 1981.

5. DAVIES, J.S. - IV. Rheumatoid factors. In: COHEN, A.S., ed. Rheumatology and immunology. New York, Grune & Stratton, 1979. p.77-80.

6. DIAMOND, L.S. - Improved method for the monoxenic cultivation of histolytica Schaudinn, 1903 and E. histolytica - like amoebae with trypanosomatids. J. Parasit., 54:715-719, 1968.

7. DIAMOND, L.S. - Techniques of axenic cultivation of Entamoeba histolytica - like amoebae. J. Parasit., 54:1074, 1968.

8. DIAMOND, L.S.; HARLOW, D.R. & CUNNICK, C.C. - A new medium for the axenic cultivation of Entamoeba histolytica and other entamoebae. Trans, roy. Soc. trop. Med. Hyg., 82:431, 1980.

9. DRESNER, E. & FROM BEY, P. - The late-fixation reaction in non-rheumatic diseases. New Engl. J. Med., 216:981-988, 1959.

10. GREEN, L.S. & PACKER, B.S. - A rose for rheumatoid factor enhancement of Plasmodium falciparum schizont inhibition in vitro. Infect. Immun.. 46:668-672, 1984.

11. GREENWOOD, B.M.; MULLER, A.S. & VALKENBURG, H.A. - Rheumatoid factor in Nigerian sera. Clin. exp. Immunol., 8:161-173, 1971.

12.HOUBA, V. & ALLISON, A.C. -IgM-antiglobulin (rheumatoid factor-like globulins) and other gammaglobulins in relation to tropical parasitic infections. Lancet, 1:848-852, 1966.

13. HUDLER, H.; STEMBERGER, H.; SCHEINER, O.; KOLLARITSCH, H. & WIEDERMANN, G. - Entamoeba histolytica. I: Mechanismen der zytotoxischen Aktion. Tropenmed. Parasit., 34:248-252, 1983.

14. HUDLER, H.; SCHEINER, O.; STEMBERGER, H; KOLLARITSCH, H. & WIEDERMANN, G. - Entamoeba histolytica. II: Einflub humoraler Immunmechanismen auf die zytotoxische Aktion. Tropenmed. Parasit., 35:5-8, 1984.

15. HULDT, G.; DAVIES, P.; ALLISON, A.C. & SCHORLEMMER, M.U. - Interaction between Entamoeba histolytica and human complement. Nature, 277:214-216, 1979.

16. KAWABATA, M.; FLORES, G.Z., IZUI, S. & KOBAYAKAWA, T. - IgM rheumatoid factors in Guatemalan onchocerciasis. Trans, roy. Soc. trop. Med. Hyg., 78:356-358, 1984.

17. KLEIN, F. & MATTERN, P. - Rheumatoid factors in primary and reactive macroglobulinaemia. Ann. rheum. Dis., 24:458-464, 1965.

18. KNIGHT, R. - An in vitro model for measuring the cytopathic effect of Entamoeba histolytica. J. Parasit., 63:388-389, 1977.

19. KOBILLR, D. & MIRELMAN, D. - Lectin activity in Entamoeba histolytica trophozoites. Infect. Immun., 29:221-225, 1980.

20. KOLLARITSCH, H.; SCHULTZ, T.H.; DIERICH, M.P.; STEMBERGER, H.; TOBISCH, P.; SCHEINER, O. & WIEDERMANN, G. - Wechselwirkung von Entamoeba histolytica mit dem humanen Komplementsystem. Mitt. öst. Ges. Tropenmed. Parasit., 7:143-150, 1985.

21. KREMSNER, P.; STEMBERGER, H.; KOLLARITSCH, H.; SCHEINER, O.; HUDLER, H. & WIEDERMANN, G. - Beeinflubung der Aktivität von Entamoeba histolytica durch Antikörper in vitro. Mitt, öst. Ges. Tropenmed. Parasit., 9:129-137, 1987.

22. LEHMAN, J.S.; HIGASHI, G.I.; BASSILY, S. & FARID, Z. - Rheumatoid factors in Salmonella and Schistosoma infections. Trans, roy. Soc. trop. Med. Hyg., 66:125-129, 1972.

23. McCAUL, T.R.; POSTON, R.N. & BIRD, R.G. - Entamoeba histolytica and E. invadens: Chromium release from labelled human liver cells in culture. Exp. Parasit., 43:342-353, 1977.

24. ORTIZ-ORTIZ, L.; ZAMACONA, G.; SEPULVEDA, B. & CAPIN, R.N. - Cell mediated immunity in patients with amoebic abscess of the liver. Clin. Immunol. Immunopath., 4:127-134, 1975.

25. RAVDIN, J.J.; CROFT, B.Y. & GUERRAND, R.L. - Cytopathogenic mechanism of Entamoeba histolytica. J. exp. Med., 152:377-390, 1980.

26. SCHEINER, O.; WIEDERMANN, G.; RUMPOLD, H.; STEINER, R.; KRAFT, D. & STEMBERGER, H. - Modulation of NK and K-cell activity by trypsin treatment of effector cells. Immunobiology, 157:343-357, 1980.

27. STEMBERGER, H. - Zytolytsche Immunreaktionen in vitro gegen Trophozoiten von Entamoeba histolytica. Immun. Infekt., 6:71-78, 1978.

28. STOCK, C; AUER, H.; PICHER, O. & SCHEINER, O. - Diagnostik der Amöbiasis mil Llilfe klassenspezifischer Antikörper. Mitt. öst. Ges. Tropenmed. Parasit., 8:23-32, 1986.

Recebido para publicação em 4/8/1988.

  • 1. BOS, H.J. - Entamoeba histolytica: cytopathogenicity of intact amoebae and cell free extracts; isolation and characterization of an intracellular toxin. Exp. Parasit., 47:369-377, 1979.
  • 2. CARSON, D.A.; BAYER, A.S.; EISENBERG, R.A.; LAW RANCE, S. & THEOFILOPOULOS, A. - IgG rheumatoid factor in subacute bacterial endocarditis: relationship to IgM rheumatoid factor and circulating immune complexes. Clin. exp. Immunol., 31:100-103, 1978.
  • 3. CARVALHO, E.M.; ANDREWS, B.S.; MARTINELLI, R.; DUTRA, M. & ROCHA, H. - Circulating immune complexes and rheumatoid factor in schistosomiasis and visceral leishmaniasis. Amer. J. trop. Med. Hyg., 32:61-68, 1983.
  • 4. CLARKSON, A.B. & MELLOW , G.H. - Rheumatoid factor - like immunoglobulin M protects preciously uninfected rat pups and dams from Trypanosoma lewi.si. Science, 214:186-188, 1981.
  • 5. DAVIES, J.S. - IV. Rheumatoid factors. In: COHEN, A.S., ed. Rheumatology and immunology. New York, Grune & Stratton, 1979. p.77-80.
  • 6. DIAMOND, L.S. - Improved method for the monoxenic cultivation of histolytica Schaudinn, 1903 and E. histolytica - like amoebae with trypanosomatids. J. Parasit., 54:715-719, 1968.
  • 7. DIAMOND, L.S. - Techniques of axenic cultivation of Entamoeba histolytica - like amoebae. J. Parasit., 54:1074, 1968.
  • 8. DIAMOND, L.S.; HARLOW, D.R. & CUNNICK, C.C. - A new medium for the axenic cultivation of Entamoeba histolytica and other entamoebae. Trans, roy. Soc. trop. Med. Hyg., 82:431, 1980.
  • 9. DRESNER, E. & FROM BEY, P. - The late-fixation reaction in non-rheumatic diseases. New Engl. J. Med., 216:981-988, 1959.
  • 10. GREEN, L.S. & PACKER, B.S. - A rose for rheumatoid factor enhancement of Plasmodium falciparum schizont inhibition in vitro. Infect. Immun.. 46:668-672, 1984.
  • 11. GREENWOOD, B.M.; MULLER, A.S. & VALKENBURG, H.A. - Rheumatoid factor in Nigerian sera. Clin. exp. Immunol., 8:161-173, 1971.
  • 12.  HOUBA, V. & ALLISON, A.C. -IgM-antiglobulin (rheumatoid factor-like globulins) and other gammaglobulins in relation to tropical parasitic infections. Lancet, 1:848-852, 1966.
  • 13. HUDLER, H.; STEMBERGER, H.; SCHEINER, O.; KOLLARITSCH, H. & WIEDERMANN, G. - Entamoeba histolytica. I: Mechanismen der zytotoxischen Aktion. Tropenmed. Parasit., 34:248-252, 1983.
  • 14. HUDLER, H.; SCHEINER, O.; STEMBERGER, H; KOLLARITSCH, H. & WIEDERMANN, G. - Entamoeba histolytica II: Einflub humoraler Immunmechanismen auf die zytotoxische Aktion. Tropenmed. Parasit., 35:5-8, 1984.
  • 15. HULDT, G.; DAVIES, P.; ALLISON, A.C. & SCHORLEMMER, M.U. - Interaction between Entamoeba histolytica and human complement. Nature, 277:214-216, 1979.
  • 16. KAWABATA, M.; FLORES, G.Z., IZUI, S. & KOBAYAKAWA, T. - IgM rheumatoid factors in Guatemalan onchocerciasis. Trans, roy. Soc. trop. Med. Hyg., 78:356-358, 1984.
  • 17. KLEIN, F. & MATTERN, P. - Rheumatoid factors in primary and reactive macroglobulinaemia. Ann. rheum. Dis., 24:458-464, 1965.
  • 18. KNIGHT, R. - An in vitro model for measuring the cytopathic effect of Entamoeba histolytica. J. Parasit., 63:388-389, 1977.
  • 19. KOBILLR, D. & MIRELMAN, D. - Lectin activity in Entamoeba histolytica trophozoites. Infect. Immun., 29:221-225, 1980.
  • 20. KOLLARITSCH, H.; SCHULTZ, T.H.; DIERICH, M.P.; STEMBERGER, H.; TOBISCH, P.; SCHEINER, O. & WIEDERMANN, G. - Wechselwirkung von Entamoeba histolytica mit dem humanen Komplementsystem. Mitt. öst. Ges. Tropenmed. Parasit., 7:143-150, 1985.
  • 21. KREMSNER, P.; STEMBERGER, H.; KOLLARITSCH, H.; SCHEINER, O.; HUDLER, H. & WIEDERMANN, G. - Beeinflubung der Aktivität von Entamoeba histolytica durch Antikörper in vitro. Mitt, öst. Ges. Tropenmed. Parasit., 9:129-137, 1987.
  • 22. LEHMAN, J.S.; HIGASHI, G.I.; BASSILY, S. & FARID, Z. - Rheumatoid factors in Salmonella and Schistosoma infections. Trans, roy. Soc. trop. Med. Hyg., 66:125-129, 1972.
  • 23. McCAUL, T.R.; POSTON, R.N. & BIRD, R.G. - Entamoeba histolytica and E. invadens: Chromium release from labelled human liver cells in culture. Exp. Parasit., 43:342-353, 1977.
  • 24. ORTIZ-ORTIZ, L.; ZAMACONA, G.; SEPULVEDA, B. & CAPIN, R.N. - Cell mediated immunity in patients with amoebic abscess of the liver. Clin. Immunol. Immunopath., 4:127-134, 1975.
  • 25. RAVDIN, J.J.; CROFT, B.Y. & GUERRAND, R.L. -  Cytopathogenic mechanism of Entamoeba histolytica. J. exp. Med., 152:377-390, 1980.
  • 26. SCHEINER, O.; WIEDERMANN, G.; RUMPOLD, H.; STEINER, R.; KRAFT, D. & STEMBERGER, H. -  Modulation of NK and K-cell activity by trypsin treatment of effector cells. Immunobiology, 157:343-357, 1980.
  • 27. STEMBERGER, H. - Zytolytsche Immunreaktionen in vitro gegen Trophozoiten von Entamoeba histolytica. Immun. Infekt., 6:71-78, 1978.
  • 28. STOCK, C; AUER, H.; PICHER, O. & SCHEINER, O. - Diagnostik der Amöbiasis mil Llilfe klassenspezifischer Antikörper. Mitt. öst. Ges. Tropenmed. Parasit., 8:23-32, 1986.
  • Address for correspondence:
    Dr. P.G. Kremsner
    State Institute of Tropical Medicine Berlin
    Königin-Elisabeth-StraBe 32 - Berlin 19, FRG

    • Publication Dates

    • Publication in this collection
      14 Oct 2011
    • Date of issue
      Aug 1989

    History

    • Received
      04 Aug 1988
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