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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.31 no.4 São Paulo July/Aug. 1989

http://dx.doi.org/10.1590/S0036-46651989000400009 

EPIDEMIOLOGIA

 

A serological investigation of rotavirus infections in a shanty town population in Rio de Janeiro

 

Investigação sorológica de infecção por rotavirus em favela do Rio de Janeiro

 

 

Rozete Silveira Azeredo; José Paulo Gagliardi Leite; Hélio Gelli Pereira; Mirían Noemi Vidal; Fritz Sutmoller; Yves Maurice; Hermann Gonçalves Schatzmayr

Departamento de Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil

Address for correspondence

 

 


SUMMARY

The presence of antibodies against rotavirus was investigated by enzyme immunosorbent assay (ELISA) in two distinct groups of children living in a shanty town in Rio de Janeiro. One hundred and thirty six plasma samples were randomly collected from children of 0 to 33 months (first group) and 255 serum samples were collected from other 85 children at ages of 2, 6 and 9 months (second group). A high percentage of antibodies were found in the newborn children and this rate decreased progressively until the age of 11 months, after which it increased again. At the age of 7 months, geometric mean antibody titers increased indicating that infection had occurred.

Key words:ELISA; Serology; Rotavirus.


RESUMO

Cento e trinta e seis plasmas coletados aleatoriamente de crianças entre 0 e 33 meses (primeiro grupo) residentes na favela de Manguinhos, Rio de Janeiro, RJ, e 255 soros coletados de 85 crianças aos 2, 6 e 9 meses de idade (segundo grupo), residentes na mesma área, foram analisados para a presença de anticorpos (classe IgG) para rotavirus por ELISA. Anticorpos foram detectados em alto percentual de crianças recém-nascidas, declinando até o 11° mês. Elevação dos anticorpos foi observada a partir do 12° mês de vida, mas houve evidências de infecção a partir do 7° mês.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

ACKNOWLEDGEMENTS

This work was supported by World Health Organization (O.M.S.), Ministério da Saúde and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). We acknowledge the technical aid of Roberto Diogo Bastos, Alexandre M. Fialho and Francisca Alves P. Santos. Thanks are also due to Valéria Gonçalves de Sá and Heliana Santos Cordeiro, for preparation of Figure 1 and typing the manuscript, respectively.

 

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Address for correspondence:
Dr. José Paulo Gagliardi Leite
Departamento de Virologia do Instituto Oswaldo Cruz
Caixa Postal 926
CEP 20010 Rio de Janeiro, RJ, Brasil

Recebido para publicação em 26/01/1989.

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