SciELO - Scientific Electronic Library Online

 
vol.31 issue5Simplification of Immune Adherence Hemagglutination test for detection of rabies antibodies in human serumFeline sporotrichosis: clinical and zoonotic aspects author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.31 no.5 São Paulo Oct. 1989

http://dx.doi.org/10.1590/S0036-46651989000500009 

ENSAIOS TERAPÊUTICOS

 

Susceptibilidad "in vitro" de cepas de Cryptococcus a 5 drogas antifungicas

 

"In vitro" susceptibility of Cryptococcus strains to 5 antifungal drugs

 

 

A. J. Bava; R. NegroniI

ICentro de Micología del Departamento de Microbiología de la Facultad de Medicina de la Universidad de Buenos Aires. Paraguay 2155. Piso II. CP. 1421. Capital Federal. Buenos Aires, Argentina

 

 


RESUMEN

Se estudió la susceptibilidad "in vitro" de 24 cepas de 3 especies del género Cryptococcus a 5 drogas antifúngicas (anfotericina B, 5 fluorocitosina, ketoconazol, itraconazol y miconazol). Las mismas se agruparon según su especie, variedad y origen de aislamiento.
Para determinar la concentración inhibitoria mínima (C.I.M.) de cada droga se empleó el método de dilución en agar con el medio básico nitrogenado para levaduras, adicionado de glucosa. Se obtuvo además la media geométrica de estos valores para cada grupo y se comparó cada uno de ellos.
Los resultados obtenidos fueron homogéneos con la sola excepción de las cepas de Cryptococcus sp (no neoformans), en las cuales se detectaron elevados valores de C.I.M. para la 5 fluorocitosina.

Unitermos: Cryptococcus; Drogas antifúngicas; Susceptibilidad "in vitro".


SUMMARY

A comparative study of the "in vitro" susceptibility of 24 Cryptococcus strains to 5 antifungal drugs (amphotericin B, 5 fluorocytosine, miconazole, itraconazole and ketoconazole), was carried out.
These strains were grouped according to species, varieties and isolation's origins. The minimum inhibitory concentration (M.I.C.) was determinated by the agar dilution technique in yeast nitrogen base agar with dextrose. The mean geometrical of the M.I.C. values of each group was compared with the others.
The results obtained were homogeneous with the only exception of the "non neoformans" strains, in which, higher M.I.C. to 5 fluorocytosine values were detected.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

AGRADECIMIENTO

A la Dra. Amalia Fernández y equipo por su gentil y desinteresada colaboración.

 

REFERENCIAS

1. AJELLO, L. - Occurrence of Cryptocoecus neoformans in soils. Amer. J. Hyg., 67: 72-77, 1958.         [ Links ]

2. BASTIDE, J. M. & MALLIE. M. - Ultraestructure et biologie de Cryptococcus neoformans. Bull. Soc. franc. Mycol. méd., 15: 11-20, 1986.         [ Links ]

3. BAVA, A. J. & NEGRONI, R. - Estudio epidemiológico sobre criptococosis en San Pedro (Buenos Aires). Rev. argent. Micol., 9: 12-16, 1986.         [ Links ]

4. BENNETT, J. E.; KWON CHUNG, K. J. & HOWARD, D. H. - Epidemiologic differences among serotypes of Cryptococcus neoformans. Amer. J. Epidem., 105: 582-586, 1977.         [ Links ]

5. DA CUNHA, T. & LUSINS, J. - Cryptococcus albidus meningitis, Sth. med. J. (Bgham, Ala.), 66: 1230-1243, 1973.         [ Links ]

6. DAMBROSI, A.; NEGRONI, R.; FAINBOIM, H. & BAVA, A. J. - Criptococosis por Cryptococcus albidus. Rev. argent. Micol., 10: 16-19, 1987.         [ Links ]

7. EMMONS, Ch. - Saprophytic sources of Cryptococcus neoformans associated with the pigeon (Columba Iivia). Amer. J. Hyg., 62: 227-232, 1955.         [ Links ]

8. FROMTLING, R. A.; ABRUZZO, G. K. & BULMER, G. S. - Cryptococcus neoformans: comparisons of "in vitro" antifungal susceptibilities of serotypes AD and BC. Mycopathologia (Den Haag), 94: 27 30, 1986.         [ Links ]

9. FROMTLING, R. A., ed. - Recent trends in the discovery, development and evaluation of antifungal agents. Barcelona, J. R. Prous Publishers, 1987.         [ Links ]

10. HENDERSON, D. K.; EDWARDS Jr., J. E.; DISMUKES, W. E. & BENNETT, J. E. - Meningitis produced by different serotypes of Cryptococcus neoformans. In: Annual Meeting of American Society of Microbiology, Dallas, Tx., 1981. Abstract. Abst. Fll.         [ Links ]

11. KWON CHUNG, K.J. - A new genus, Filobasidiella, the perfect state of Cryptococcus neoformans. Mycologia, 67: 1197-1200, 1975.         [ Links ]

12. KWON CHUNG, K. J.; BENNETT, J. E. & RHODES, J. C. - Taxonomic studies on Filobasidiella species and their anamorphs. Antonie van Leeuwenhoek, 48: 25 28, 1982.         [ Links ]

 

 

Recebido para publicação em 28/3/1989.

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License