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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.32 no.5 São Paulo Sept./Oct. 1990

https://doi.org/10.1590/S0036-46651990000500009 

SEROLOGY

 

Comparison of indirect immunofluorescence test for measles antibodies with haemagglutination inhibition and plaque neutralization tests

 

Comparação da reação de imunofluorescência indireta para o vírus do sarampo com as reações de inibição da hemaglutinação e neutralização por redução de placas

 

 

Vanda Akico Ueda Fick de SouzaI; Cláudio Sérgio PannutiI; Cid Vieira Franco de GodoyII; Paul AlbrechtIII; Marta Heloísa LopesII; João da Silva MendonçaIV

IInstituto de Medicina Tropical de São Paulo (LIM/52-HC-FM-USP)
IIFaculdade de Medicina da Universidade de São Paulo
IIIDivision of Virology, FDA, Bethesda
IVHospital do Servidor Público do Estado de São Paulo

Address for correspondence

 

 


SUMMARY

Indirect Immunofluorescence (IFA), Plaque Reduction Neutralization (PRN) and Haemagglutination Inhibition (HI) tests for measles antibodies were carried out in 197 sera obtained from umbilical cord and vaccinated children. The IFA was also applied to blood samples collected with filter paper.
IFA results demonstrated that the test is relatively simple to perform, with good reproducibility for different antigen lots. Good correlation was obtained between IFA, PRN and HI antibody titers. Better correlation was demonstrated with IFA and PRN than with HI and PRN tests.
Sensitivity of IFA in detecting antibody was less effective than PRN, however more effective than HI using rhesus monkey red blood cells. PRN antibody titers over 100 were detected by IFA but not by HI (9.7% with negative results). IFA may be of considerable practical use and able to substitute HI in Seroepidemiological surveys and to evaluate vaccine efficacy. It also can be simplified by employing filter paper collected samples.

Key words: Measles; Indirect immunofluorescence; Plaque reduction, Haemagglutination inhibiton.


RESUMO

As reações de imunofluorescência indireta (RIF), neutralização por redução de placas (RNP) e inibição da hemaglutinação (RIH) para detecção de anticorpos para o vírus do sarampo foram aplicadas a 197 soros provenientes de cordão umbilical e de crianças vacinadas contra o sarampo. Avaliou-se ainda a aplicação da RIF em amostras colhidas em papel de filtro.
A RIF apresentou-se como uma prova de execução relativamente simples, de boa reprodutibilidade com diferentes partidas de antígeno. Observou-se boa correlação entre os títulos de anticorpos obtidos por RIF, RNP e RIH. Com a RNP, a RIF apresentou maior correlação que a RIH.
A sensibilidade da RIF na detecção de anticorpos contra o sarampo foi menor que RNP, porém superior à da RIH com hemácias de macaco rhesus.
Anticorpos com títulos superiores a 100 pela RNP foram sistematicamente detectados pela RIF, mas não por RIH (9,7% de resultados negativos). A RIF pode ser de grande utilidade, podendo substituir com vantagem a RIH em inquéritos soroepidemiológicos e na avaliação de eficácia de vacinas. Além disso, mostrou-se adequada para aplicação em larga escala, já que permite processar amostras de sangue colhidas em papel de filtro.


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 

 

REFERENCES

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Address for correspondence:
Vanda Akico Ueda Fick de Souza
Instituto de Medicina Tropical de São Paulo
Av. Dr. Enéas de Carvalho Aguiar, 470
05403 São Paulo, SP, Brasil

Recebido para publicação em 23/11/1989.

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