Schistosoma mansoni: acquired immunity in mice after the use of oxamniquine at the evolutive skin and pulmonary phases

Coelho, Paulo Marcos Z.; Mello, Rômulo T.; Gerken, Sílvia E.

doi:10.1590/S0036-46651991000100006

Abstracts

Mice infected with 350 cercariae of Schistosoma mansoni (LE strain) were treated with oxamniquine, at the dose of 400 mg/kg, 24, 48, 72, and 96 h after infection. Forty days after the treatment, the animals were submitted to a challenge infection with 80 cercariae, through the abdominal and ear skins. The number of immature worms in the animal groups treated 24 and 96 h after the first infection was found to be lower than that in the control group, thus showing that the death of schisto-somes by chemotherapy, at the skin and pulmonary phases, causes an acquired resistance state.

Schistosoma mansoni; Acquired immunity; Oxamniquine

Camundongos infectados com 350 cercárias de Schistosoma mansoni (cepa LE) foram tratados com oxamniquina, em dose única de 400 mg/kg, 24,48,72 e 96 horas após a infecção. Quarenta dias após o tratamento, os animais foram submetidos a uma infecção desafio com 80 cercárias, através da pele abdominal e da orelha. O número de vermes imaturos nos grupos de animais tratados 24 e 96 horas após a primeira infecção foi menor do que o do grupo controle, evidenciando que a morte de esquistossômulos por quimioterapia, durante as fases da pele e do pulmão, causa um estado de resistência adquirida.

IMMUNOLOGY

Schistosoma mansoni: acquired immunity in mice after the use of oxamniquine at the evolutive skin and pulmonary phases

Schistosoma mansoni: imunidade adquirida em camundongos, após o uso de oxamniquina durante as fases evolutivas da pele e do pulmão

Paulo Marcos Z. Coelho^I; Rômulo T. Mello^II; Sílvia E. Gerken^II

^IDepartamento de Parasitologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais (ICB/UFMG). Belo Horizonte, Minas Gerais, Brasil

^IIDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia da Universidade Federal de Minas Gerais. Belo Horizonte, Minas Gerais, Brasil

^{Address for correspondence} Address for correspondence: Prof. Paulo Marcos Z. Coelho Deptº Parasitología, ICB/UFMG Caixa Postal 2486 30161 Belo Horizonte, MG, Brasil

SUMMARY

Mice infected with 350 cercariae of Schistosoma mansoni (LE strain) were treated with oxamniquine, at the dose of 400 mg/kg, 24, 48, 72, and 96 h after infection. Forty days after the treatment, the animals were submitted to a challenge infection with 80 cercariae, through the abdominal and ear skins. The number of immature worms in the animal groups treated 24 and 96 h after the first infection was found to be lower than that in the control group, thus showing that the death of schisto-somes by chemotherapy, at the skin and pulmonary phases, causes an acquired resistance state.

Key words: Schistosoma mansoni; Acquired immunity; Oxamniquine.

RESUMO

Camundongos infectados com 350 cercárias de Schistosoma mansoni (cepa LE) foram tratados com oxamniquina, em dose única de 400 mg/kg, 24,48,72 e 96 horas após a infecção. Quarenta dias após o tratamento, os animais foram submetidos a uma infecção desafio com 80 cercárias, através da pele abdominal e da orelha. O número de vermes imaturos nos grupos de animais tratados 24 e 96 horas após a primeira infecção foi menor do que o do grupo controle, evidenciando que a morte de esquistossômulos por quimioterapia, durante as fases da pele e do pulmão, causa um estado de resistência adquirida.

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ACKNOWLEDGMENTS

We would like to thank Mrs. Vera de Paula Ribeiro for translating the manuscript; Mr. Alberto G. dos Santos, Mrs. Alice Neni Faria, Mr. José de Souza Filho and Miss Zenir de Souza for excellent technical assistance.

Recebido para publicação em 11/6/1990.

Aceito para publicação em 16/10/1990.

1. BARBOSA, M.A.; PELLEGRINO, J.; COELHO, P.M.Z. & MACHADO, I.B.M. Quantitative aspects of the asynchronism in development of Schistosoma mansoni in mice. Rev. Inst. Med. trop. S. Paulo, 20: 121-132, 1978.
2. BICKLE, Q.D. & ANDREWS, B.J. Resistance following drug attenuation (Ro-11.3128 or oxamniquine) of early Schistosoma mansoni infections in mice. Parasitology, 90: 325-338, 1985.
3. BICKLE, Q.D. & JAMES, E.R. Immunization of mice using radiation attenuated cryopreserved schistosomula of Schistosoma mansoni. Trans. roy. Soc. trop. Med. Hyg., 72: 677-678, 1978.
4. COULSON, P.S. & MOUNTFORD, A.P. Fate of attenuated schistosomula administered to mice by different routes, relative to the immunity induced against Schistosoma mansoni. Parasitology, 99: 39-45, 1989.
5. DEAN, D.A. A Review. Schistosoma and related genera: acquired resistance in mice. Exp. Parasit., 55: 1-104, 1983.
6. ELSAGHIER, A.A.F. & McLAREN, D.J. Schistosoma mansoni: Influence of immunization and challenge schedules on the sites and mechanisms of resistance in CBA/Ca mice vaccinated with highly irradiated cercariae. J. Helmint., 63: 173-190, 1989.
7. ERICKSON, D.G. & CALDWELL, W.L. Acquired resistance in mice and rats after exposure to gamma-irradiated cercariae. Amer. J. trop. Med. Hyg., 14: 566-573, 1965.
8. FLISSER, A. & McLAREN, D.J. Effect of praziquantel treatment on lung-stage larvae of Schistosoma mansoni in vivo. Parasitology, 98: 203-211, 1989.
9. GERKEN, S.E.; OLIVEIRA, A.F.S.; CORREA-OLIVEIRA, R. & MOTTA-SANTOS, T.A. Schistosoma mansoni: infecçăo experimental de camundongos através da orelha e quantificaçăo do parasitismo na pele. Rev. Inst. Med. trop. S. Paulo, 28: 381-388, 1986.
10. MASTIN, A.J.; WILSON, R.A. & BICKLE, Q.D. Induction of resistance to Schistosoma mansoni in mice by chemotherapy: migration of schistosomula in primary and challenge infection. Parasitology, 90: 519-528, 1985.
11. MENSON, E.N.; COULSON, P.S. & WILSON, R.A. Schistosoma mansoni: circulating and pulmonary leukocyte responses related to the induction of protective immunity in mice by irradiated parasites. Parasitology, 90: 43-45, 1989.
12. MOUNTFORD, A.P.; COULSON, P.S.; SAUNDERS, N. & WILSON, R.A. Characteristics of protective immunity in mice induced by drug-attenuated larvae of Schistosoma mansoni Antigen localization and antibody responses. J. Immunol., 143: 989-995, 1989.
13. PELLEGRINO, J.; OLIVEIRA, C.A.; FARIA, J. & CUNHA, A.S. New approach to the screening of drugs in experimental schistosomiasis mansoni in mice. Amer. J. trop. Med. Hyg., 11: 201-215, 1962.
14. PELLEGRINO, J. & SIQUEIRA, A.F. Técnica de perfusăo para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.
15. PERLOWAGORA-SZUMLEWICZ, A. & OLIVIER, L. Schistosoma mansoni: development of challenge infections in mice exposed to irradiated cercariae. Science, 140: 411-412, 1963.
16. RADKE, M.G. & SADUN, E.H. Resistance produced in mice by exposure to irridiated Schistosoma mansoni cercariae. Exp. Parasit., 13: 134-142, 1963.
17. SNEDECOR, G.W. & COCHRAN, W.G. Two-way classification. In: SNEDECOR, G.W. & COCHRAN, G., ed. Statistical methods. Iowa, The Iowa State University Press, 1971. V. 2, cap. 11, p. 327-329.
18. VIGNALI, D.A.A.; KLAUS, S.N.; BICKLE, Q.D. & TAYLOR, M.G. Histological examination of the cellular reactions around schistosomula of Schistosoma mansoni in the lungs of sublethally irradiated and non-irradiated, immune and control rats. Parasitology, 98: 57-65,1989.
19. VILLELLA, J.B.; GOMBERG, H.J. & GOULD, S.E. Immunization to Schistosoma mansoni in mice inoculated with radiated cercariae. Science, 134: 1073-1075, 1961.

Address for correspondence:

Prof. Paulo Marcos Z. Coelho

Deptº Parasitología, ICB/UFMG

Caixa Postal 2486

30161 Belo Horizonte, MG, Brasil

Publication Dates

Publication in this collection
12 Sept 2006
Date of issue
Feb 1991

History

Accepted
16 Oct 1990
Received
11 June 1990

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BARBOSA, M.A.; PELLEGRINO, J.; COELHO, P.M.Z. & MACHADO, I.B.M. Quantitative aspects of the asynchronism in development of Schistosoma mansoni in mice. Rev. Inst. Med. trop. S. Paulo, 20: 121-132, 1978.

[2] 2. BICKLE, Q.D. & ANDREWS, B.J. Resistance following drug attenuation (Ro-11.3128 or oxamniquine) of early Schistosoma mansoni infections in mice. Parasitology, 90: 325-338, 1985.

[3] 3. BICKLE, Q.D. & JAMES, E.R. Immunization of mice using radiation attenuated cryopreserved schistosomula of Schistosoma mansoni. Trans. roy. Soc. trop. Med. Hyg., 72: 677-678, 1978.

[4] 4. COULSON, P.S. & MOUNTFORD, A.P. Fate of attenuated schistosomula administered to mice by different routes, relative to the immunity induced against Schistosoma mansoni. Parasitology, 99: 39-45, 1989.

[5] 5. DEAN, D.A. A Review. Schistosoma and related genera: acquired resistance in mice. Exp. Parasit., 55: 1-104, 1983.

[6] 6. ELSAGHIER, A.A.F. & McLAREN, D.J. Schistosoma mansoni: Influence of immunization and challenge schedules on the sites and mechanisms of resistance in CBA/Ca mice vaccinated with highly irradiated cercariae. J. Helmint., 63: 173-190, 1989.

[7] 7. ERICKSON, D.G. & CALDWELL, W.L. Acquired resistance in mice and rats after exposure to gamma-irradiated cercariae. Amer. J. trop. Med. Hyg., 14: 566-573, 1965.

[8] 8. FLISSER, A. & McLAREN, D.J. Effect of praziquantel treatment on lung-stage larvae of Schistosoma mansoni in vivo. Parasitology, 98: 203-211, 1989.

[9] 9. GERKEN, S.E.; OLIVEIRA, A.F.S.; CORREA-OLIVEIRA, R. & MOTTA-SANTOS, T.A. Schistosoma mansoni: infecçăo experimental de camundongos através da orelha e quantificaçăo do parasitismo na pele. Rev. Inst. Med. trop. S. Paulo, 28: 381-388, 1986.

[10] 10. MASTIN, A.J.; WILSON, R.A. & BICKLE, Q.D. Induction of resistance to Schistosoma mansoni in mice by chemotherapy: migration of schistosomula in primary and challenge infection. Parasitology, 90: 519-528, 1985.

[11] 11. MENSON, E.N.; COULSON, P.S. & WILSON, R.A. Schistosoma mansoni: circulating and pulmonary leukocyte responses related to the induction of protective immunity in mice by irradiated parasites. Parasitology, 90: 43-45, 1989.

[12] 12. MOUNTFORD, A.P.; COULSON, P.S.; SAUNDERS, N. & WILSON, R.A. Characteristics of protective immunity in mice induced by drug-attenuated larvae of Schistosoma mansoni Antigen localization and antibody responses. J. Immunol., 143: 989-995, 1989.

[13] 13. PELLEGRINO, J.; OLIVEIRA, C.A.; FARIA, J. & CUNHA, A.S. New approach to the screening of drugs in experimental schistosomiasis mansoni in mice. Amer. J. trop. Med. Hyg., 11: 201-215, 1962.

[14] 14. PELLEGRINO, J. & SIQUEIRA, A.F. Técnica de perfusăo para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.

[15] 15. PERLOWAGORA-SZUMLEWICZ, A. & OLIVIER, L. Schistosoma mansoni: development of challenge infections in mice exposed to irradiated cercariae. Science, 140: 411-412, 1963.

[16] 16. RADKE, M.G. & SADUN, E.H. Resistance produced in mice by exposure to irridiated Schistosoma mansoni cercariae. Exp. Parasit., 13: 134-142, 1963.

[17] 17. SNEDECOR, G.W. & COCHRAN, W.G. Two-way classification. In: SNEDECOR, G.W. & COCHRAN, G., ed. Statistical methods. Iowa, The Iowa State University Press, 1971. V. 2, cap. 11, p. 327-329.

[18] 18. VIGNALI, D.A.A.; KLAUS, S.N.; BICKLE, Q.D. & TAYLOR, M.G. Histological examination of the cellular reactions around schistosomula of Schistosoma mansoni in the lungs of sublethally irradiated and non-irradiated, immune and control rats. Parasitology, 98: 57-65,1989.

[19] 19. VILLELLA, J.B.; GOMBERG, H.J. & GOULD, S.E. Immunization to Schistosoma mansoni in mice inoculated with radiated cercariae. Science, 134: 1073-1075, 1961.

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