Enzyme-linked immunosorbent assay (ELISA) for measles antibody: a comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

Souza, Vanda Akico Ueda Fick de; Pannuti, Claudio Sergio; Sumita, Laura Massami; Albrecht, Paul

doi:10.1590/S0036-46651991000100007

Abstracts

An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copo-sitivities of 93.3% and 82.7%. The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.

Measles; ELISA; Plaque neutralization; Immunofluorescence; Hemagglutination inhibition

A reação imunoenzimática (ELISA) para determinação de anticorpos para o vírus do sarampo foi comparada com a reação de neutralização de placas (RNP), inibição da hemaglutinação (RIH) e imunofluorescência indireta (RIF). Das 179 amostras positivas pela RNP, somente 2, com títulos iguais a 8, se apresentaram negativas por ELISA (copositividade de 98.9%). A RIF e RIH apresentaram, respectivamente, copositividade de 93.3 e 82.7%. ELISA apresentou sensibilidade equivalente à complexa RNP, boa reprodutibilidade e representa uma alternativa para a detecção de baixos títulos de anticorpos contra o sarampo.

IMMUNOLOGY

Enzyme-linked immunosorbent assay (ELISA) for measles antibody. A comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

Reação imunoenzimática (ELISA) para detecção de anticorpos para o vírus do sarampo. Comparação com reações de inibição da hema-glutinação, imunofluorescência indireta e neutralização de placas

Vanda Akico Ueda Fick de Souza^I; Claudio Sergio Pannuti^I; Laura Massami Sumita^I; Paul Albrecht^II

^IInstituto de Medicina Tropical de São Paulo. (LIM-52 HC-FMUSP). São Paulo, SP, Brazil

^IIDivision of Virology. FDA, Bethesda, USA

^{Address for correspondence} Address for correspondence: Vanda Akico Ueda Fick de Souza Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470 CEP 05403 São Paulo, SP, Brazil

SUMMARY

An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copo-sitivities of 93.3% and 82.7%.

The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.

Key words: Measles; ELISA; Plaque neutralization; Immunofluorescence; Hemagglutination inhibition.

RESUMO

A reação imunoenzimática (ELISA) para determinação de anticorpos para o vírus do sarampo foi comparada com a reação de neutralização de placas (RNP), inibição da hemaglutinação (RIH) e imunofluorescência indireta (RIF). Das 179 amostras positivas pela RNP, somente 2, com títulos iguais a 8, se apresentaram negativas por ELISA (copositividade de 98.9%). A RIF e RIH apresentaram, respectivamente, copositividade de 93.3 e 82.7%. ELISA apresentou sensibilidade equivalente à complexa RNP, boa reprodutibilidade e representa uma alternativa para a detecção de baixos títulos de anticorpos contra o sarampo.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENTS

We thank Dr. H. F. Andrade Jr., Mrs. Maria Cristina D. S. Fink, Almir Robson Ferreira and Mrs. Jussara Clemente S. P. Moraes for their valuable cooperation.

Recebido para publicação em 11/6/1990.

Aceito para publicação em 18/9/1990.

1. ALBRECHT, P.; HERRMANN, K. & BURNS, G.R. - Role of virus strain in conventional and enhanced measles plaque neutralization test. J. virol. Meth., 3: 251-260, 1981.
2. BOTELER, W.L.; LUIPERSBECK, P.M.; FUCCILLO, D.A. & O'BEIRNE, A.J. Enzyme-linked immunosorbent assay for detection of measles antibody. J. clin. Microbiol., 17: 814-818, 1983.
3. GERSHON, A.A. & KRUGMAN, S. Measles virus. In: LENNETTE, E.H. & SCHMIDT, N.S., ed. Diagnostic procedures for viral and rickettsial infections. 5th ed. New York, American Public Health Association, 1979. p. 665-693.
4. KAHANE, S.; GOLDSTEIN, V. & SAROV, I. Detection of IgG antibodies specific for measles virus by Enzyme-linked immunosorbent assay (ELISA). Intervirology, 12: 39-46, 1979.
5. KLEIMAN, M.B.; BLACKBURN, C.K.L.; ZIMMERMAN, S.E. & FRENCH, M.L.V. Comparison of Enzyme-linked immunosorbent assay for acute measles with Haemagglutination inhibition, Complement fixation, and Fluorescent-antibody methods. J. clin. Microbiol., 14: 147-152, 1981.
6. KRUGMAN, S.; GILES, J.P.; FRIEDMAN, H. & STONE, S. Studies on immunity to measles. J. Pediat., 66: 471-488, 1965.
7. LOWRY, O.H.; ROSEBROUGH, N.J.; FARR, A.L. & RANDALL, R.J. Protein measurement with the Folin phenol reagent. J. biol. Chem., 193: 265-275, 1951.
8. MARKS, J.S.; HAYDEN, G.F. & ORESTEIN, W.A. Methodologic issues in the evaluation of vaccine effectiveness. Amer. J. Epidem., 116: 510-522, 1982.
9. ORESTEIN, W.; ALBRECHT, P.; HERRMANN, K.L.; BERNIER, R.; BART, K.J. & ROVIRA, E.Z. The plaque neutralization test as a measure of prior exposure to measles virus. J. infect. Dis., 155: 146-149, 1987.
10. PANNUTI, C.S.; SOUZA, V.A.U.F.; TAKAOKA, N.; LEME, S.T.S.; PEREIRA, C.R.; CARVALHO, R.P.S. & AMATO NETO, V. Interferęncia entre vacinas anti-sarampo e antipoliomielite. Bol. Ofic. sanit. panamer., 103: 227-232, 1987.
11. SOUZA, V.A.U.F.; PANNUTI, C.S.; GODOY, C.V.F.: ALBRECHT, P.; LOPES, M.H. & MENDONÇA, J.S. Comparison of indirect immunofluorescence test for measles antibodies with Haemagglutination inhibition and Plaque neutralization test. Rev. Inst. Med. trop. S. Paulo, 31 (supl. 7): S80, 1989.
12. VOLLER, A. & BIDWELL, D.E. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Brit. J. exp. Path., 57: 243-247, 1976.
13. WEIGLE, K.A.; MURPHY, M.D. & BRUNELL, P.A. Enzyme-linked immunosorbent assay for evaluation of immunity to measles virus. J. clin. Microbiol., 19: 376-379, 1984.

Address for correspondence:

Vanda Akico Ueda Fick de Souza

Instituto de Medicina Tropical de São Paulo

Av. Dr. Enéas de Carvalho Aguiar, 470

CEP 05403 São Paulo, SP, Brazil

Publication Dates

Publication in this collection
12 Sept 2006
Date of issue
Feb 1991

History

Accepted
18 Sept 1990
Received
11 June 1990

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALBRECHT, P.; HERRMANN, K. & BURNS, G.R. - Role of virus strain in conventional and enhanced measles plaque neutralization test. J. virol. Meth., 3: 251-260, 1981.

[2] 2. BOTELER, W.L.; LUIPERSBECK, P.M.; FUCCILLO, D.A. & O'BEIRNE, A.J. Enzyme-linked immunosorbent assay for detection of measles antibody. J. clin. Microbiol., 17: 814-818, 1983.

[3] 3. GERSHON, A.A. & KRUGMAN, S. Measles virus. In: LENNETTE, E.H. & SCHMIDT, N.S., ed. Diagnostic procedures for viral and rickettsial infections. 5th ed. New York, American Public Health Association, 1979. p. 665-693.

[4] 4. KAHANE, S.; GOLDSTEIN, V. & SAROV, I. Detection of IgG antibodies specific for measles virus by Enzyme-linked immunosorbent assay (ELISA). Intervirology, 12: 39-46, 1979.

[5] 5. KLEIMAN, M.B.; BLACKBURN, C.K.L.; ZIMMERMAN, S.E. & FRENCH, M.L.V. Comparison of Enzyme-linked immunosorbent assay for acute measles with Haemagglutination inhibition, Complement fixation, and Fluorescent-antibody methods. J. clin. Microbiol., 14: 147-152, 1981.

[6] 6. KRUGMAN, S.; GILES, J.P.; FRIEDMAN, H. & STONE, S. Studies on immunity to measles. J. Pediat., 66: 471-488, 1965.

[7] 7. LOWRY, O.H.; ROSEBROUGH, N.J.; FARR, A.L. & RANDALL, R.J. Protein measurement with the Folin phenol reagent. J. biol. Chem., 193: 265-275, 1951.

[8] 8. MARKS, J.S.; HAYDEN, G.F. & ORESTEIN, W.A. Methodologic issues in the evaluation of vaccine effectiveness. Amer. J. Epidem., 116: 510-522, 1982.

[9] 9. ORESTEIN, W.; ALBRECHT, P.; HERRMANN, K.L.; BERNIER, R.; BART, K.J. & ROVIRA, E.Z. The plaque neutralization test as a measure of prior exposure to measles virus. J. infect. Dis., 155: 146-149, 1987.

[10] 10. PANNUTI, C.S.; SOUZA, V.A.U.F.; TAKAOKA, N.; LEME, S.T.S.; PEREIRA, C.R.; CARVALHO, R.P.S. & AMATO NETO, V. Interferęncia entre vacinas anti-sarampo e antipoliomielite. Bol. Ofic. sanit. panamer., 103: 227-232, 1987.

[11] 11. SOUZA, V.A.U.F.; PANNUTI, C.S.; GODOY, C.V.F.: ALBRECHT, P.; LOPES, M.H. & MENDONÇA, J.S. Comparison of indirect immunofluorescence test for measles antibodies with Haemagglutination inhibition and Plaque neutralization test. Rev. Inst. Med. trop. S. Paulo, 31 (supl. 7): S80, 1989.

[12] 12. VOLLER, A. & BIDWELL, D.E. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Brit. J. exp. Path., 57: 243-247, 1976.

[13] 13. WEIGLE, K.A.; MURPHY, M.D. & BRUNELL, P.A. Enzyme-linked immunosorbent assay for evaluation of immunity to measles virus. J. clin. Microbiol., 19: 376-379, 1984.

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