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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.33 no.3 São Paulo May/June 1991

http://dx.doi.org/10.1590/S0036-46651991000300002 

ARTIGOS ORIGINAIS

 

Deteccion de citomegalovirus mediante la tecnica de inmunoperoxidasa y aislamiento viral

 

Cytomegalovirus detection by Immunoperoxidase assay and viral isolation

 

 

Maritza Alvarez; Miguel Marrero; Angel Valdivia; Serafina Garcia; Odalys Valdes; Luis Morier

Departamento de Virología. Instituto de Medicina Tropical "Pedro Kourí", Siboney, Ciudad Habana, Cuba

Dirección para correspondencia

 

 


RESUMEN

En el presente estudio se comparó la técnica de inmunoperoxidasa para la detección de citomegalovirus (IPCMV) utilizando anticuerpos monoclonales que reconocen proteínas precoces virales con el método convencional de aislamiento viral en fibroblastos humanos. Un total de 150 muestras de orina fueron examinadas encontrando una sensibilidad de un 89.8% y una especificidad de 91.3% de la técnica de IPCMV comparada con el aislamiento viral. Una de las ventajas que presentó la IPCMV fue la rapidez con que fueron obtenidos los resultados (48 horas) mientras que el aislamiento viral fue como promedio 14 días.

Unitermos: Citomegalovirus; Inmunoperoxidasa; Aislamiento viral.


SUMMARY

An Immunoperoxidase assay was applied to detect early antigens of Cytomegalovirus (CMV) in 150 urine samples from immunocompromised patients, using the commercial available monoclonal antibody against CMV El3. The detection of early antigen by IP (IPCMV) is compared to the conventional cell culture isolation regarding specificity and sensitivity in order to evaluate is usefulness in the diagnostic of CMV infections. The IPCMV showed a sensitivity of 89.8% and a specificity of 91.3% when compared to the isolation method. The great advantage of the IPCMV is based on the shorter time results are achieved, since 48-72 Hs can be enough to provide evidence of CMV infection, while in the isolation technique cytopatho-genic effect was present around 14 days after sample inoculation.


 

 

Texto completo disponible sólo en PDF.

Full text available only in PDF format.

 

 

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Dirección para correspondencia:
Dra. M. Alvarez
Instituto de Medicina Tropical "Pedro Kourí"
Apartado Postal Nº 601
Marianao, C. Habana, Cuba

Recebido para publicação em 16/05/1990
Aceito para publicação em 18/03/1991

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