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Revista do Instituto de Medicina Tropical de São Paulo

Print version ISSN 0036-4665

Rev. Inst. Med. trop. S. Paulo vol.33 no.4 São Paulo July/Aug. 1991

http://dx.doi.org/10.1590/S0036-46651991000400007 

SOROLOGIA

 

E.L.I.S.A. en coccidioidomicosis humana

 

E.L.I.S.A. in human coccidioidomycosis

 

 

Iris Nora Tiraboschi; B. Marticorena; R. Negroni

Centro de Micología del Departamento de Microbiología. Facultad de Medicina de Buenos Aires. (U.B.A.). Paraguay 2155. Piso 11. C.P. 1121. Buenos Aires. Argentina

 

 


RESUMEN

Se realizó E.L.I.S.A. con exoantígeno de Coccidioides immitis para la detectión de anticuerpos, en 67 sueros humanos diluidos 1/1000, 1/2000, 1/4000 y 1/8000. De los 18 sueros de enfermos de coccidioidomicosis comprobada por examen directo, cultivo y/o histología, 5 fueron negativos, en otros 13 fueron positivos en una o varias diluciones. 3/26 sueros de personas sanas, coccidioidino positivas, fueron positivos en títulos de 1/1000 y el resto no tuvo anticuerpos detectables. No presentaron reacciones positivas ninguno de los sueros controles de personas sanas, pero sí lo hicieron 4/8 pacientes con otras micosis.
Se concluye que E.L.I.S.A. es útil para la detección de mínimas cantidades de anticuerpos o en sueros que no pueden ser procesados por fijación de complemento. No es recomendable el uso de la técnica en forma aislada por al presencia de reacciones cruzadas.

Unitermos: Coccidioidomicosis; E.L.I.S.A.; Coccidioides immitis.


SUMMARY

An E.L.I.S.A. test for antibody detection, with an exo-antigen of Coccidioides immitis was standardized in 67 humans sera diluited in 1/1000, 1/2000, 1/4000 and 1/8000. Eightheen sera from mycologically proved cases of coccidioidomycosis were studied: 5 were negative and 13 were positive in some dilutions. 3/26 sera of healthy persons who presented positive skin tests with coccidioidin were positive and the other 23 sera did not have positive reactions. None of the 15 sera of healthy human exhibited positive E.L.I.S.A. Serum samples of 8 patients suffering other deep mycosis were studied, 4 of them presented cross-reactions in E.L.I.S.A. tests. E.L.I.S.A. test seems to be a useful Serologic technique for antibody detection in anticomplementary serum samples or when a low concentration of antibodies should be detected. As it is very sensitive, cross-reactions with other mycoses are frequent, thus the use other more specific serologic technique together E.L.I.S.A. is recommended.


 

 

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AGRADECIMIENTOS

Agradecemos la ayuda brindada por los Dres. Kantor, I., Barrera, V., Ritacco, V. y Torrrea, G., del C.E.P.A.N.Z.O. Dr. Castiglia, V. del Hospital de Clínicas y a nuestros compañeros de trabajo del Departamento de Microbiología de la U.B.A.

 

REFERENCIAS BIBLIOGRAFICAS

1. BARRERA, L.; RITACCO, V.; ELISELE, C.; PALESCHI, A.; MONTEVERDE, A.; TORREA, G.; NEGRONI, R.; PADULA, E.; BATTAGLIA, A.; GONZALEZ, L. & KANTOR, I. - Evaluación del Enzimoinmunoensayo para el diagnóstico rápido de la tuberculosis paucibacilar del adulto. Medicina (B. Aires), 49: 561-566, 1989.         [ Links ]

2. BROUWER, J. - Detection of antibodies against Aspergillus fumigatus comparison between double immunodiffusion, E.L.I.S.A. and immunoblot analysis. Int. Arch. Allergy, 85: 244-249, 1988.         [ Links ]

3. CANO, L.; BRUMMER, E.; STEVENS, D. & RESTREPO, A. - An evaluation of the enzyme-linked immunoadssorbent assay (E.L.I.S.A.) for quantitalion of antibodies to Paracoccidioides brasiliensis. J. med. vet. Mycology, 24: 467-475, 1986.         [ Links ]

4. CASTAÑED, E.; BRUMMER, E.; PAPPAGIANIS, D. & STEVENS, D.A. - Impairment of cellular but not humoral immune responses in chronicopulmonary and disseminated paracoccidioidomycosis in mice. Infect. Immun., 56: 1771-1777, 1988.         [ Links ]

5. COLE, G.; KIRKLAND, T.; FRANCO, M.; ZHU, S.; YUAN, L.; SUN, S.H. & HEARN, V. - Immunoreactivity of a surface wall fraction produced by spherules of Coccidioides immitis. Infect. Immun., 56: 2695-2701, 1988.         [ Links ]

6. CONANT, N.F.; SMITH, D.T.; BAKER, R.D. & CALLAWAY, J.L. - Manual of clinical mycology. 3rd. ed. Philadelphia, W.B. Saunders, 1971.         [ Links ]

7. DAY, M.J. & PENHALE, W.J. - Humoral immunity in disseminated Aspergillus terreus infection in the dog. Vet. Microbiol., 16: 283-294, 1988.         [ Links ]

8. FUJITA, S.; MATSUBARA, F. & MATSUDA, T. - Demonstration of antigenemia in patients with invasive aspergillosis by biotinstreptaridin enzyme-linked immunosorbent assay. J. Lab. clin. Med., 112: 464-470, 1988.         [ Links ]

9. GEORGE, R.; LABERT, R.S.; BRUCE, M.J.; PICKERING, J.W. & WALCOTT, N.Z. - Radioimmunoassay: a sensitive screening test for histoplasmosis and blastomicosis. Amer. Rev. resp. Dis., 124: 407-410, 1981.         [ Links ]

10. GIMENO, S.R. - Puesta a punto de la técnica de E.L.I.S.A. en coccidioidomicosis experimental. Rev. argent. Micol., 9: 25-27, 1986.         [ Links ]

11. GRAYBILL, J.R.; PATINO, M. & AHRENS, J. - In situ localization of Histoplasma capsulatum using colloidal gold immune electron microscopy. Mycopathologia (Den Haag), 104: 181-188, 1988.         [ Links ]

12. GREENFIELD, R.A.; TRUTT, D.L.; RICKARD, R.C. & ALTMILLER, D.H. - Comparison of antibody, antigen and metabolite assays in rat models of systemic and gastrointestinal candidiasis. J. clin. Microbiol., 26: 409-417, 1988.         [ Links ]

13. NEGRONI, R.; FINQUELIEVICH, J.L. & ELÍAS COSTA, M.R. - Estudio de la coccidioidomicosis experimental en ratas Wistar. Rev. argent. Micol., 8: 7-11, 1985.         [ Links ]

14. PATTERSON, T.F.; MINITER, P.; RYAN, J.L. & ANDRIOLE, U.T. - Effect of immunosuppression and amphotericin B on Aspergillus antigenemia in an experimental model. J. infect. Dis, 158: 415-422, 1988.         [ Links ]

15. PAVLIAK, V. & SANDULA, J. - Cross-reactivity of pathogenic Candida mannans studied by enzyme-linked immunosorbent assay (E.L.I.S.A.) and precipitin methods. Mycoses, 31: 34-39, 1988.         [ Links ]

16. PAVLIAK, V.; SAUNDULA, J.; TOMSIROVA, A. & GALLO, J. - Determination of antibodies to Candida albicans cell wall components by immunodiffusion, E.L.I.S.A. and its rapid modification. Mycoses, 31: 426-432, 1988.         [ Links ]

17. PINON, J.M.; THOANNES, M.; POIRRIEZ, J.; BOULANT, J. & LEPAN, H. - Enzyme-linked immuno-filtration assay (E.L.I.S.A.) for the detection of IgG, IgM, IgA and IgE antibodies against Aspergillus fumigatus. J. med. vet. Mycol., 25: 77-83, 1987.         [ Links ]

18. REPENTIGNY, L. & REISS, E. - Current trends in immunodiagnosis of candidiasis and aspergillosis. Rev. infect. Dis., 6: 301-312, 1984.         [ Links ]

19. RIPPON, J.W. - Medical mycology. The pathogenic fungi and the pathogenic actinomycetes. 3rd. ed. Philadelphia, W.B. Saunders Company, 1988.         [ Links ]

20. RUBINSTEIN, P. & NEGRONI, R. - Micosis broncopulmonares del adulto y del niño. 2da. ed. Buenos Aires, Editorial Beta, 1981.         [ Links ]

21. SERRANO, M.; de ALBORNOZ, M.B. & WRICH, M. - E.L.I.S.A. in the follow up of deep mycoses. In: I.S.H.A.M. Congress. Atlanta, 1985.         [ Links ]

22. SUZUKI, H.; TAGUCHI, H.; MISHIMURA, R.; MIYAJI, M.; NAKAMURA, A. & NAKASIMA, H. - Studies on detection of Candida antigen in the sera of mice inoculated orally with Candida albicans. Mycopathologia (Den Haag), 104: 7-17, 1988.         [ Links ]

23. TOLLEMAR, J.; HOLMBERG, K.; RINGDEN, O. & LONNQUIST, B. - Surveillance tests for the diagnosis of invasive fungal infections in bone marrow transplant recipients. Scand. J. infect. Dis., 21: 205-212, 1989.         [ Links ]

24. TOJO, M.; SHIBATA, N.; OJANAI, T.; MIKAMI, T.; SUZUKI, M. & SUZUKI, S. - Quantitative precipitin reaction and enzyme-linked immunosorbent assay of mannans of Candida albicans NIH A-207 and NIH B-792 strains compared. Clin. Chem., 34: 2423-2425, 1988.         [ Links ]

25. WACK, E.E.; DUGGER, K.O. & GALGIANI, J.N. - Enzyme-linked immunosorbent assay for antigens of Coccidioides immitis: human sera interference corrected by acidification-heat extraction. J. Lab. clin. Med., 111: 560-565, 1988.         [ Links ]

26. YAMAMOTO, S.; TOIDA, I.; WADA, M.; MOSOJINA, S. & KUDOU, S. - Serological diagnosis of pulmonary aspergillosis by E.L.I.S.A. Kekkaku, 64: 15-24, 1989.         [ Links ]

 

 

Recebido para publicação em 09/08/1990
Aceito para publicação em 28/05/1990