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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.33 no.5 São Paulo Sept./Oct. 1991 



Clonal variation within a mucosal isolate derived from a patient with Leishmania (Viannia) braziliensis infection


Variação clonal de um isolado derivado de um paciente com infecção mucosa pela Leishmania (Viannia) braziliensis



César Augusto Cuba-CubaI; David EvansII; Ana de Cassia RosaI; Philip Davis MarsdenI

IUniversity of Brasilia - Núcleo de Medicina Tropical e Nutrição. CEP 70910, Brasília, DF, Brazil
IIDepartment of Medical Parasitology - London School of Hygiene and Tropical Medicine. London, England




Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and scrodemes analysis.
Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated clear differences on Cellulose Acetate (CA) and thin starch gel electrophoresis. Also a panel of specific monoclonal antibodies showed such differences.
Our observations provide additional evidence that Leishmania (Viannia) braziliensis is composed by subpopulations of parasites with peculiar biochemical and antigenic characteristics.

Key words: Leishmania (Viannia) braziliensis; Clonal Variation; Zymodemes; Serodemes.


No transcurso de um período de 5 anos foram estudados 3 isolados de um paciente com leishmaniose mucosa recidivante causada pela Leishmania (Viannia) braziliensis e 7 clones de um desses isolados. Este estudo foi feito pela análise dos serodemas e zimodemas.
Os resultados indicaram a ocorrência de variações fenotípicas clonais. Oito marcadores isoenzimáticos demonstraram diferenças nos padrões eletroforéticos em Acetato de Celulose (AC), bem como em camada fina de amido. Da mesma forma foram consultadas diferenças em um painel de anticorpos monoclonais específicos e subespecíficos.
Nossas observações indicam ainda que a Leishmania (Viannia) braziliensis está composta por subpopulações de parasitas com características bioquímicas e antigênicas peculiares.




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Recebido para publicação em 24/01/1991.
Aceito para publicação em 06/06/1991.
Partially supported by CNPq, grant nº 300796-82 BM.

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