Clonal variation within a mucosal isolate derived from a patient with Leishmania (Viannia) braziliensis infection

Cuba-Cuba, César Augusto; Evans, David; Rosa, Ana de Cassia; Marsden, Philip Davis

doi:10.1590/S0036-46651991000500002

Abstracts

Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and scrodemes analysis. Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated clear differences on Cellulose Acetate (CA) and thin starch gel electrophoresis. Also a panel of specific monoclonal antibodies showed such differences. Our observations provide additional evidence that Leishmania (Viannia) braziliensis is composed by subpopulations of parasites with peculiar biochemical and antigenic characteristics.

Leishmania (Viannia) braziliensis; Clonal Variation; Zymodemes; Serodemes

No transcurso de um período de 5 anos foram estudados 3 isolados de um paciente com leishmaniose mucosa recidivante causada pela Leishmania (Viannia) braziliensis e 7 clones de um desses isolados. Este estudo foi feito pela análise dos serodemas e zimodemas. Os resultados indicaram a ocorrência de variações fenotípicas clonais. Oito marcadores isoenzimáticos demonstraram diferenças nos padrões eletroforéticos em Acetato de Celulose (AC), bem como em camada fina de amido. Da mesma forma foram consultadas diferenças em um painel de anticorpos monoclonais específicos e subespecíficos. Nossas observações indicam ainda que a Leishmania (Viannia) braziliensis está composta por subpopulações de parasitas com características bioquímicas e antigênicas peculiares.

ORIGINAL ARTICLES

Clonal variation within a mucosal isolate derived from a patient with Leishmania (Viannia) braziliensis infection

Variação clonal de um isolado derivado de um paciente com infecção mucosa pela Leishmania (Viannia) braziliensis

César Augusto Cuba-Cuba^I; David Evans^II; Ana de Cassia Rosa^I; Philip Davis Marsden^I

^IUniversity of Brasilia - Núcleo de Medicina Tropical e Nutrição. CEP 70910, Brasília, DF, Brazil

^IIDepartment of Medical Parasitology - London School of Hygiene and Tropical Medicine. London, England

SUMMARY

Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and scrodemes analysis.

Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated clear differences on Cellulose Acetate (CA) and thin starch gel electrophoresis. Also a panel of specific monoclonal antibodies showed such differences.

Our observations provide additional evidence that Leishmania (Viannia) braziliensis is composed by subpopulations of parasites with peculiar biochemical and antigenic characteristics.

Key words: Leishmania (Viannia) braziliensis; Clonal Variation; Zymodemes; Serodemes.

RESUMO

No transcurso de um período de 5 anos foram estudados 3 isolados de um paciente com leishmaniose mucosa recidivante causada pela Leishmania (Viannia) braziliensis e 7 clones de um desses isolados. Este estudo foi feito pela análise dos serodemas e zimodemas.

Os resultados indicaram a ocorrência de variações fenotípicas clonais. Oito marcadores isoenzimáticos demonstraram diferenças nos padrões eletroforéticos em Acetato de Celulose (AC), bem como em camada fina de amido. Da mesma forma foram consultadas diferenças em um painel de anticorpos monoclonais específicos e subespecíficos.

Nossas observações indicam ainda que a Leishmania (Viannia) braziliensis está composta por subpopulações de parasitas com características bioquímicas e antigênicas peculiares.

Recebido para publicação em 24/01/1991.

Aceito para publicação em 06/06/1991.

Partially supported by CNPq, grant nº 300796-82 BM.

1. CUBA-CUBA, C.A.; MARSDEN, P.D.; BARRETO, A.C.; ROCHA, R.; SAMPAIO, R. & PATZLAFF, L. - Parasitologic and Immunologic diagnosis of American (Mucocutaneous) Leishmaniasis. Bull. Pan-Amer. Hlth. Org., 15: 249-259, 1981.
2. DVORAK, J. - Single cell isolates of Trypanosoma cruzi How and Why? Rev. Soc. bras. Med. trop., 18 (Supl.): 15-24, 1985.
3. EVANS, D.A.; LANHAM, J.M.; BALDWIN, C.I. & PETERS, W. - The isolation and isoenzyme characterization of Leishmania braziliensis subspecies from patients with cutaneous leishmaniasis acquired in Belize. Trans. roy. Soc. trop. Med. Hyg., 78: 35-42, 1984.
4. KREUTZER, R.D. & CIIRISTENSEN, H.A. - Characterization of Leishmania spp. by isoenzyme electrophoresis. Amer. J. trop. Med. Hyg., 29: 199-208, 1980.
5. LE BLANCQ, S.M.; SCHNUR, L.F. & PETERS, W. - Leishmania in the Old World. 1. The geographical and hostal distribution of L. major zymodemes. Trans. roy. Soc. trop. Med. Hyg., 80: 99-112, 1986a.
6. LE BLANCQ, S.M.; BELEHU, A. & PETERS, W. - Leishmania in the Old World. 3. The distribution of L. aethiopica zymodemes. Trans. roy. Soc. trop. Med. Hyg., 80: 360-366, 1986b.
7. LOPES, U.G.; MOMEM, H.; GRIMALDI Jr., G.; MARZOCHI, M.C.; PACHECO, R.S. & MOREL, C.M. - Schizodeme and zymodeme characterization of Leishmania in the investigation of foci of visceral and cutaneous leishmaniasis. J. Parasit., 70: 89-98, 1984.
8. LLANOS CUENTAS, E.A.; MARSDEN, P.D.; LAGO, L.; BARRETO, A.C.; CUBA-CUBA, C.A. & JOHNSON, W.D. - Human Mucocutaneous Leishmaniasis in Tręs Braços, Bahia, Brazil. An area of Leishmania braziliensis transmission. II. Cutaneous disease. Presentation and evolution. Rev. Soc. bras. Med. trop., 17: 169-177, 1984.
9. MARSDEN, P.D.; SAMPAIO, R.N.R.; CARVALHO, E.M.; VEIGA, J.P.T.; COSTA, L.M. & LLANOS CUENTAS, E.A. - High continuous antimony therapy in two patients with unresponsive mucosal leishmaniasis. Amer. J. trop. Med. Hyg., 34: 710-713, 1985.
10. MARSDEN, P.D. - Mucosal Leishmaniasis (espundia Escomel 1911). Trans. roy. Soc. trop. Med. Hyg., 80: 859-876, 1986.
11. McMAHON-PRATT, D.; JAFFE, C.L. & GRIMALDI Jr., G. - Application of Monoclonal Antibodies to the classification of Leishmania species. In: MOREL, C.M., ed. Genes and antigens of parasites: a laboratory manual. 2nd. ed. Rio de Janeiro, FIOCRUZ, 1984. p. 121-135.
12. PACHECO. R.S.; LOPES, U.G.; MOREL, C.M.; GRIMALDI Jr., G. & MOMEM, H. - Schizodeme analysis of Leishmania isolates and comparison with some phenotypic techniques. In: RIOUX, J.A., ed. Leishmania. Taxonomie et phylogenese. Aplications ecoepidemiologiques. Montpellier, CNRS/INSERN/OMS, 1986. p. 57-65.
13. SARAVIA, N.G.; HOLGUÍN, A.F.; McMAHON-PRATT, D. & D'ALESSANDRO, A. - Mucocutaneous leishmaniasis in Colombia: Leishmania braziliensis subspecies diversity. Amer. J. trop. Med. Hyg., 34: 714-720, 1985.
14. WALTON, B.C.; SHAW, J.J. & LAINSON, R. - Observations on the "in vitro" cultivation of Leishmania braziliensis. J. Parasit., 63: 1118-1119, 1977.

Publication Dates

Publication in this collection
06 Sept 2006
Date of issue
Oct 1991

History

Received
24 Jan 1991
Accepted
06 June 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. CUBA-CUBA, C.A.; MARSDEN, P.D.; BARRETO, A.C.; ROCHA, R.; SAMPAIO, R. & PATZLAFF, L. - Parasitologic and Immunologic diagnosis of American (Mucocutaneous) Leishmaniasis. Bull. Pan-Amer. Hlth. Org., 15: 249-259, 1981.

[2] 2. DVORAK, J. - Single cell isolates of Trypanosoma cruzi How and Why? Rev. Soc. bras. Med. trop., 18 (Supl.): 15-24, 1985.

[3] 3. EVANS, D.A.; LANHAM, J.M.; BALDWIN, C.I. & PETERS, W. - The isolation and isoenzyme characterization of Leishmania braziliensis subspecies from patients with cutaneous leishmaniasis acquired in Belize. Trans. roy. Soc. trop. Med. Hyg., 78: 35-42, 1984.

[4] 4. KREUTZER, R.D. & CIIRISTENSEN, H.A. - Characterization of Leishmania spp. by isoenzyme electrophoresis. Amer. J. trop. Med. Hyg., 29: 199-208, 1980.

[5] 5. LE BLANCQ, S.M.; SCHNUR, L.F. & PETERS, W. - Leishmania in the Old World. 1. The geographical and hostal distribution of L. major zymodemes. Trans. roy. Soc. trop. Med. Hyg., 80: 99-112, 1986a.

[6] 6. LE BLANCQ, S.M.; BELEHU, A. & PETERS, W. - Leishmania in the Old World. 3. The distribution of L. aethiopica zymodemes. Trans. roy. Soc. trop. Med. Hyg., 80: 360-366, 1986b.

[7] 7. LOPES, U.G.; MOMEM, H.; GRIMALDI Jr., G.; MARZOCHI, M.C.; PACHECO, R.S. & MOREL, C.M. - Schizodeme and zymodeme characterization of Leishmania in the investigation of foci of visceral and cutaneous leishmaniasis. J. Parasit., 70: 89-98, 1984.

[8] 8. LLANOS CUENTAS, E.A.; MARSDEN, P.D.; LAGO, L.; BARRETO, A.C.; CUBA-CUBA, C.A. & JOHNSON, W.D. - Human Mucocutaneous Leishmaniasis in Tręs Braços, Bahia, Brazil. An area of Leishmania braziliensis transmission. II. Cutaneous disease. Presentation and evolution. Rev. Soc. bras. Med. trop., 17: 169-177, 1984.

[9] 9. MARSDEN, P.D.; SAMPAIO, R.N.R.; CARVALHO, E.M.; VEIGA, J.P.T.; COSTA, L.M. & LLANOS CUENTAS, E.A. - High continuous antimony therapy in two patients with unresponsive mucosal leishmaniasis. Amer. J. trop. Med. Hyg., 34: 710-713, 1985.

[10] 10. MARSDEN, P.D. - Mucosal Leishmaniasis (espundia Escomel 1911). Trans. roy. Soc. trop. Med. Hyg., 80: 859-876, 1986.

[11] 11. McMAHON-PRATT, D.; JAFFE, C.L. & GRIMALDI Jr., G. - Application of Monoclonal Antibodies to the classification of Leishmania species. In: MOREL, C.M., ed. Genes and antigens of parasites: a laboratory manual. 2nd. ed. Rio de Janeiro, FIOCRUZ, 1984. p. 121-135.

[12] 12. PACHECO. R.S.; LOPES, U.G.; MOREL, C.M.; GRIMALDI Jr., G. & MOMEM, H. - Schizodeme analysis of Leishmania isolates and comparison with some phenotypic techniques. In: RIOUX, J.A., ed. Leishmania. Taxonomie et phylogenese. Aplications ecoepidemiologiques. Montpellier, CNRS/INSERN/OMS, 1986. p. 57-65.

[13] 13. SARAVIA, N.G.; HOLGUÍN, A.F.; McMAHON-PRATT, D. & D'ALESSANDRO, A. - Mucocutaneous leishmaniasis in Colombia: Leishmania braziliensis subspecies diversity. Amer. J. trop. Med. Hyg., 34: 714-720, 1985.

[14] 14. WALTON, B.C.; SHAW, J.J. & LAINSON, R. - Observations on the "in vitro" cultivation of Leishmania braziliensis. J. Parasit., 63: 1118-1119, 1977.

Brasil

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Clonal variation within a mucosal isolate derived from a patient with Leishmania (Viannia) braziliensis infection

Variação clonal de um isolado derivado de um paciente com infecção mucosa pela Leishmania (Viannia) braziliensis

Abstracts

Publication Dates

History