Plasmodium vinckei petteri: algunos aspectos de su esporogonia y esquizogonia exoeritrocitaria

Montalvo Alvarez, Ana M.; Landau, Irene; Baccam, Doanh

doi:10.1590/S0036-46651991000600001

Resúmenes

Por medio de la infección experimental de Anopheles stephensi, a partir de ratones portadores de gametocitos de Plasmodium vinckei petteri, se realizó el estudio morfológico de la esporogonia, que comprende: la descripción del ooquineto, la evolución completa de los ooquistes y su transformación final en esporozoitos. Estos fueron empleados más tarde para infectar por via intravenosa nuevos ratones, a los que se realizó biopsias sucesivas, para el estudio de los esquizontes exoeritrocíticos, cuya morfología no difere de la de otras especies del grupo. Se determinó la duración mínima del ciclo hepático, que resultó ser de 61 horas. Estos datos, junto a otros que refieren las características del ciclo hemático, ayudan a completar la información que se tiene sobre la especie, que la hacen recomendable para su utilización como modelo experimental en el estudio de la malaria humana.

Plasmodium vinckei petteri; Esporogonia; Esquizogonia exoeritrocitaria; Ooquineto; Ooquiste; Esporozoitos; Esquizontes exoeritrocíticos

A study of Plasmodium vinckei peterri sporogony was performed by experimental infection of Anopheles stephensi with gametocytes from infected mice. The study includes the description of the ookinete, complete evolution of oocysts and their final transformation to sporozoites. These were later used for intravenous infection of new mice, in order to study the exoerythrocytic schizogony. The morphology of exoerythrocytic schizonts was similar to that of other species of the same group. The minimal duration of the hepatic cycle was found to be of 61 hours. These data, along with other related to the hematic cycle characteristics, help to complete the information on the species, which can be recommended as a model for the study of human malaria.

ARTIGOS ORIGINAIS

Plasmodium vinckei petteri: algunos aspectos de su esporogonia y esquizogonia exoeritrocitaria

Plasmodium vinckei petteri: some aspects of its sporogony and exoerythrocytic schizogony

Ana M. Montalvo Alvarez^I; Irene Landau^II; Doanh Baccam^II

^ILaboratorio de Protozoología. Instituto Medicina Tropical "Pedro Kouri" Ministerio de la Salud Publica. Apdo. 601 Zona Postal Marianao 13. Habana, Cuba

^IILaboratoire de Zoologie-Vers. Museo Nacional de Historia Natural. Paris, Francia

RESUMEN

Por medio de la infección experimental de Anopheles stephensi, a partir de ratones portadores de gametocitos de Plasmodium vinckei petteri, se realizó el estudio morfológico de la esporogonia, que comprende: la descripción del ooquineto, la evolución completa de los ooquistes y su transformación final en esporozoitos. Estos fueron empleados más tarde para infectar por via intravenosa nuevos ratones, a los que se realizó biopsias sucesivas, para el estudio de los esquizontes exoeritrocíticos, cuya morfología no difere de la de otras especies del grupo. Se determinó la duración mínima del ciclo hepático, que resultó ser de 61 horas. Estos datos, junto a otros que refieren las características del ciclo hemático, ayudan a completar la información que se tiene sobre la especie, que la hacen recomendable para su utilización como modelo experimental en el estudio de la malaria humana.

Key words: Plasmodium vinckei petteri; Esporogonia; Esquizogonia exoeritrocitaria; Ooquineto; Ooquiste; Esporozoitos; Esquizontes exoeritrocíticos.

SUMMARY

A study of Plasmodium vinckei peterri sporogony was performed by experimental infection of Anopheles stephensi with gametocytes from infected mice. The study includes the description of the ookinete, complete evolution of oocysts and their final transformation to sporozoites. These were later used for intravenous infection of new mice, in order to study the exoerythrocytic schizogony. The morphology of exoerythrocytic schizonts was similar to that of other species of the same group. The minimal duration of the hepatic cycle was found to be of 61 hours. These data, along with other related to the hematic cycle characteristics, help to complete the information on the species, which can be recommended as a model for the study of human malaria.

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Recebido para publicação em 20/12/1989.

Aceito para publicação em 30/4/1991.

1. BAFORT, J. - Étude du cycle biologique du Plasmodium vinckei vinckei Rodhain, 1952. Ann. Soc. beige Med. trop., 49: 553-628, 1969.
2. BAFORT, J. - Le cycle biologique du Plasmodium vinckei de Nigeria. In: Multicolloque Europeen de Parasitologie, ler., Rennes, 1971. Compte Rendu. p. 235-237.
3. CARTER, R. & WALLIKER, D. - New observations on the malaria parasites of rodents of the Central African Republic. Plasmodium vinckei petteri subsp. nov. and Plasmodium chabaudi Landau, 1965. Ann. trop. Med. Parasit., 69: 187-196, 1975.
4. COPPEL, R.L.; SAINT, R.B.; STAHL, H.D.; LANGFORD, C.J.; BROWN, G.V.; ANDERS, R.F. & KEMP, D.J. - Plasmodium falciparum: Differentiation of isolates with DNA Hibridization using antigen gene probes. Exp. Parasit., 60: 82-89, 1985.
5. FUCHAROEN, S.; TIRAWANCHAI, N.; WILAIRAT, P.; PANYIM, S. & THAITHONG, S. - Differentiaton of Plasmodium falciparum clones by means of a repetitive DNA probe. Trans. roy. Soc. trop. Med. Hyg., 82: 209- 211, 1988.
6. HILL, J. - The activity of drug combination against established infections of rodents malaria. Parasitology, 95: 17-23, 1987.
7. KILLICK-KENDRICK, R. - Parasitic protozoa in the blood of rodents. I. The life-cycle and zoo-geography of Plasmodium berghei nigeriensis subsp. nov. Ann. trop. Med. Parasit., 67: 261-277, 1973.
8. KILLICK-KENDRICK, R. - Parasitic protozoa of the blood of rodents. V. Plasmodium vinckei brucechwatti subsp. nov. A malaria parasite the ticket rat, Thamnomys rutilans, in Nigeria. Ann. Parasit. hum. comp., 50: 251-264, 1975.
9. LANDAU, I. -Description de Plasmodium chabaudi n. sp., parasite de rongeurs africain. C.R. Acad. Sci. (Paris), 260: 3758-3761, 1980.
10. LANDAU, I. & CHABAUD, A.G. - Nouvelles donnees sur le cycle nycthéméral des Plasmodium. CR. Acad. Sci. (Paris), 291 (Ser.D): 985-988, 1980.
11. LANDAU, I.: MICHEL, J.C.; ADAM, J.P. & BOULARD, Y. - The life cycle of Plasmodium vinckei lentum subsp nov. in the laboratory; comments on the nomenclature of the murine malaria parasites. Ann. trop. Med. Parasit., 64: 315-323, 1970.
12. MONTALVO ALVAREZ, A.M.: LANDAU, I.; BACCAM, D.; Chabaud, A.G.& Gingsburg, H. - Experimental modifications of the circadian rythm of Plasmodium vinckei petteri following cryopreservation; probable resistance of the merozoite to thawing. C.R. Acad. Sci. ( Paris ), S.III, 307: 5-10, 1988.
13. OO, M. - Isoenzyme variation in schizonts of Plasmodium vivax from Burma. Trans. roy Soc. trop. Med. Hyg., 8: 1-4, 1986.
14. SANDERSON, A.; WALLIKER, D. & MOLEZ, J.F. - Enzyme typing of Plasmodium falciparum from African and some other Old World countries. Trans. roy Soc. trop. med. Hyg. 75: 263-267, 1981.
15. SHUTE, P.G. & MARYON, M. - Laboratory techniques for the study of malaria. London, J. and A. Churchill, 1966.
16. WALLIKER, D. - Characterization of Plasmodium falciparum of differents countries. Ann Soc. belge. Med. trop., 65: 69-75, 1985.
17. WISSR, M.F. & PLITT, B. - Plasmodium berghei, Plasmodium chabaudi and Plasmodium falciparum: Similarities in phosphoproteins and protein kinase activities and their stage specific expression. Exp. Parasit., 64: 328-335, 1987.

Fechas de Publicación

Publicación en esta colección
11 Set 2006
Fecha del número
Dic 1991

Histórico

Recibido
20 Dic 1989
Acepto
30 Abr 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BAFORT, J. - Étude du cycle biologique du Plasmodium vinckei vinckei Rodhain, 1952. Ann. Soc. beige Med. trop., 49: 553-628, 1969.

[2] 2. BAFORT, J. - Le cycle biologique du Plasmodium vinckei de Nigeria. In: Multicolloque Europeen de Parasitologie, ler., Rennes, 1971. Compte Rendu. p. 235-237.

[3] 3. CARTER, R. & WALLIKER, D. - New observations on the malaria parasites of rodents of the Central African Republic. Plasmodium vinckei petteri subsp. nov. and Plasmodium chabaudi Landau, 1965. Ann. trop. Med. Parasit., 69: 187-196, 1975.

[4] 4. COPPEL, R.L.; SAINT, R.B.; STAHL, H.D.; LANGFORD, C.J.; BROWN, G.V.; ANDERS, R.F. & KEMP, D.J. - Plasmodium falciparum: Differentiation of isolates with DNA Hibridization using antigen gene probes. Exp. Parasit., 60: 82-89, 1985.

[5] 5. FUCHAROEN, S.; TIRAWANCHAI, N.; WILAIRAT, P.; PANYIM, S. & THAITHONG, S. - Differentiaton of Plasmodium falciparum clones by means of a repetitive DNA probe. Trans. roy. Soc. trop. Med. Hyg., 82: 209- 211, 1988.

[6] 6. HILL, J. - The activity of drug combination against established infections of rodents malaria. Parasitology, 95: 17-23, 1987.

[7] 7. KILLICK-KENDRICK, R. - Parasitic protozoa in the blood of rodents. I. The life-cycle and zoo-geography of Plasmodium berghei nigeriensis subsp. nov. Ann. trop. Med. Parasit., 67: 261-277, 1973.

[8] 8. KILLICK-KENDRICK, R. - Parasitic protozoa of the blood of rodents. V. Plasmodium vinckei brucechwatti subsp. nov. A malaria parasite the ticket rat, Thamnomys rutilans, in Nigeria. Ann. Parasit. hum. comp., 50: 251-264, 1975.

[9] 9. LANDAU, I. -Description de Plasmodium chabaudi n. sp., parasite de rongeurs africain. C.R. Acad. Sci. (Paris), 260: 3758-3761, 1980.

[10] 10. LANDAU, I. & CHABAUD, A.G. - Nouvelles donnees sur le cycle nycthéméral des Plasmodium. CR. Acad. Sci. (Paris), 291 (Ser.D): 985-988, 1980.

[11] 11. LANDAU, I.: MICHEL, J.C.; ADAM, J.P. & BOULARD, Y. - The life cycle of Plasmodium vinckei lentum subsp nov. in the laboratory; comments on the nomenclature of the murine malaria parasites. Ann. trop. Med. Parasit., 64: 315-323, 1970.

[12] 12. MONTALVO ALVAREZ, A.M.: LANDAU, I.; BACCAM, D.; Chabaud, A.G.& Gingsburg, H. - Experimental modifications of the circadian rythm of Plasmodium vinckei petteri following cryopreservation; probable resistance of the merozoite to thawing. C.R. Acad. Sci. ( Paris ), S.III, 307: 5-10, 1988.

[13] 13. OO, M. - Isoenzyme variation in schizonts of Plasmodium vivax from Burma. Trans. roy Soc. trop. Med. Hyg., 8: 1-4, 1986.

[14] 14. SANDERSON, A.; WALLIKER, D. & MOLEZ, J.F. - Enzyme typing of Plasmodium falciparum from African and some other Old World countries. Trans. roy Soc. trop. med. Hyg. 75: 263-267, 1981.

[15] 15. SHUTE, P.G. & MARYON, M. - Laboratory techniques for the study of malaria. London, J. and A. Churchill, 1966.

[16] 16. WALLIKER, D. - Characterization of Plasmodium falciparum of differents countries. Ann Soc. belge. Med. trop., 65: 69-75, 1985.

[17] 17. WISSR, M.F. & PLITT, B. - Plasmodium berghei, Plasmodium chabaudi and Plasmodium falciparum: Similarities in phosphoproteins and protein kinase activities and their stage specific expression. Exp. Parasit., 64: 328-335, 1987.

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Plasmodium vinckei petteri: algunos aspectos de su esporogonia y esquizogonia exoeritrocitaria

Plasmodium vinckei petteri: some aspects of its sporogony and exoerythrocytic schizogony

Resúmenes

Fechas de Publicación

Histórico