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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.33 no.6 São Paulo Nov./Dec. 1991

http://dx.doi.org/10.1590/S0036-46651991000600010 

SOROLOGIA

 

UltramicroELISA para la detección de anticuerpos IgM anti M. leprae

 

UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

 

 

José LaferteI; Elba G. AbreuI; René RobainaII; Vicente VerezIII

IInstituto de Medicina Tropical "Pedro Kourí" Apdo. 601 Siboney, Ciudad Habana, Cuba
IICentro de Inmunoensayo. Apdo. 6945. Ciudad Habana, Cuba
IIILaboratorio de Química de los Carbohidratos. Universidad de la Habana. Facultad de Química. Ciudad Habana, Cuba

Dirección para correspondencia

 

 


RESUMEN

La disponibilidad del sistema Ultramicroanalítico (SUMA) y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100), un grupo de pacientes tuberculosos (50), un grupo de enfermos de lepra (65) y un grupo de contactos de estos enfermos (50), mostró la especificidad del ensayo para evidenciar la infección con el M. leprae.
Los resultados obtenidos del estudio adicional de 140 muestras de suero de contactos de enfermos estuvieron estrechamente correlacionados (r = 0,98) con los resultados obtenidos por la técnica de microELISA convencional.
La utilización del SUMA no solo permite un notable ahorro de reactivos si no además facilita la lectura, cálculo, validación y almacenamiento automático de los resultados.

Unitermos: UltramicroELISA


SUMMARY

The availability of an ultramicroanalitic system (SUMA) and specie-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstrate the infection with M. leprae was corroborated through a screening of 433 blood bank serum samples and other 265 from diferent groups (100, control group, 50 tuberculosis patients, 65 leprosy patients, 50 from household). The results obtained in the aditional study of 140 household sero showed a high correlation (r = 0.98) with the conventional microELISA method. The use of SUMA allows saving reagents and time since sample handling, plate reading, print out and storing the data are computer assisted.


 

 

Texto completo disponible sólo en PDF.

Full text available only in PDF format.

 

 

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Dirección para correspondencia:
Lic. José Laferté
Instituto de Medicina Tropical "Pedro Kourí"
Apdo. 601
Siboney, Ciudad de la Habana, Cuba

Recebido para publicação em 16/05/1990.
Aceito para publicação em 01/04/1991.

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