Comparison on the performance of Leishmania major-like and Leishmania braziliensis braziliensis as antigen for new world leishmaniasis IgG-immunofluorescence test

Guimarães, Maria Carolina Soares; Celeste, Beatriz J.; Corrales L., Edelma María; Antunes, Carlos M.F.

doi:10.1590/S0036-46651991000600012

Abstracts

The performance of an antigen of L. major-like promastigotes for the serological diagnosis of mucocutaneous leishmaniasis in the IgG-immunofluorescent test was compared to that of an antigen of L. braziliensis braziliensis. Each antigen was used to test two hundred and twenty-four sera of etiologies such as mucocutaneous leishmaniasis, deep mycoses, toxoplasmosis, malaria, Chagas' disease, visceral leishmaniasis, anti-nuclear factor, schistosomaiasis, rheumatoid factor and normal controls. Agreement between responses to each antigen was high: 77.2% of leishmaniases sera agreed on a positive or a negative result to both antigens and 91.1 % of control sera. Cross reactivity was restricted to Chagas' disease sera, visceral leishmaniasis, anti-nuclear factor and paracoccidiodomycosis. The quantitative response of leishmaniasis and Chagas' disease sera to both antigens was evaluated by a linear regression; although the y-intercept and the slope were different for each antigen, neither was better than the other in the disclosure of anti-Leishmania antibodies. In the case of Chagas' disease sera the L. major-like antigen was better than L. b. braziliensis' to disclose cross-reacting antibodies.

L. major-like; braziliensis braziliensis; IgG; immunofluorescence test

O desempenho de um antígeno de promastigotas de L. major-like para o diagnóstico sorológico de leishmaniose mucocutânea pelo teste de imunofluorescência-IgG foi comparado com o desempenho de um antígeno de L. braziliensis braziliensis. Cada antígeno foi usado para testar 224 soros de etiologias como leishmaniose mucocutânea, micoses profundas, toxoplasmose, malária, doença de Chagas, leishmaniose visceral, fator anti-núcleo, esquistossomose mansônica, fator reumatóide e controles normais. A concordância entre as respostas a cada antígeno foi grande: 77,2% dos soros de leishmaniose mostraram resultado positivo para ambos os antígenos ou negativo, assim como 91,1% dos soros controle negativos. Reações cruzadas ficaram restritas à doença de Chagas, leishmaniose visceral, fator anti-núcleo e paracoccidioidomicose. A resposta quantitativa dos soros de leishmaniose e doença de Chagas foi avaliada pelo método de regressão linear; embora a intercessão com o eixo y e o "slope" fossem diferentes para cada antígeno nenhum deles se mostrou melhor na evidenciação de anticorpos anti-Leishmania. O antígeno de L. major-like mostrou-se melhor que o de L. b. braziliensis na evidenciação de anticorpos em soros de doença de Chagas.

SEROEPIDEMIOLOGY

Comparison on the performance of Leishmania major-like and Leishmania braziliensis braziliensis as antigen for new world leishmaniasis IgG-immunofluorescence test

Comparação do desempenho de antígenos de Leishmania major-like e Leishmania braziliensis braziliensis no teste de imunofluorescência-IgG para leishmaniose mucocutânea

Maria Carolina Soares Guimarães^I,II; Beatriz J. Celeste^II; Edelma María Corrales L.^II; Carlos M.F. Antunes^III

^IDepartamento de Medicina Preventiva, Faculdade de Medicina Universidade de São Paulo

^IILaboratório de Soroepidemiologia, Instituto de Medicina Tropical de São Paulo

^IIIDepartamento de Parasitología, Instituto de Ciências Biomédicas, Universidade Federal de Minas Gerais

^{Address for correspondence} Address for correspondence: Dr. Maria Carolina S. Guimarães Laboratório de Soroepidemiologia, Instituto de Medicina Tropical de S. Paulo Av. Dr. Eneas de Carvalho Aguiar, 470 05403, S. Paulo, Brazil

SUMMARY

The performance of an antigen of L. major-like promastigotes for the serological diagnosis of mucocutaneous leishmaniasis in the IgG-immunofluorescent test was compared to that of an antigen of L. braziliensis braziliensis. Each antigen was used to test two hundred and twenty-four sera of etiologies such as mucocutaneous leishmaniasis, deep mycoses, toxoplasmosis, malaria, Chagas' disease, visceral leishmaniasis, anti-nuclear factor, schistosomaiasis, rheumatoid factor and normal controls. Agreement between responses to each antigen was high: 77.2% of leishmaniases sera agreed on a positive or a negative result to both antigens and 91.1 % of control sera.

Cross reactivity was restricted to Chagas' disease sera, visceral leishmaniasis, anti-nuclear factor and paracoccidiodomycosis. The quantitative response of leishmaniasis and Chagas' disease sera to both antigens was evaluated by a linear regression; although the y-intercept and the slope were different for each antigen, neither was better than the other in the disclosure of anti-Leishmania antibodies. In the case of Chagas' disease sera the L. major-like antigen was better than L. b. braziliensis' to disclose cross-reacting antibodies.

Key words: L. major-like; braziliensis braziliensis; IgG; immunofluorescence test.

RESUMO

O desempenho de um antígeno de promastigotas de L. major-like para o diagnóstico sorológico de leishmaniose mucocutânea pelo teste de imunofluorescência-IgG foi comparado com o desempenho de um antígeno de L. braziliensis braziliensis. Cada antígeno foi usado para testar 224 soros de etiologias como leishmaniose mucocutânea, micoses profundas, toxoplasmose, malária, doença de Chagas, leishmaniose visceral, fator anti-núcleo, esquistossomose mansônica, fator reumatóide e controles normais. A concordância entre as respostas a cada antígeno foi grande: 77,2% dos soros de leishmaniose mostraram resultado positivo para ambos os antígenos ou negativo, assim como 91,1% dos soros controle negativos. Reações cruzadas ficaram restritas à doença de Chagas, leishmaniose visceral, fator anti-núcleo e paracoccidioidomicose. A resposta quantitativa dos soros de leishmaniose e doença de Chagas foi avaliada pelo método de regressão linear; embora a intercessão com o eixo y e o "slope" fossem diferentes para cada antígeno nenhum deles se mostrou melhor na evidenciação de anticorpos anti-Leishmania. O antígeno de L. major-like mostrou-se melhor que o de L. b. braziliensis na evidenciação de anticorpos em soros de doença de Chagas.

Full text available only in PDF format.

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ACKNOWLEDGEMENTS

The authors wish to thank Mr. Paulo de Oliveira for excellent technical assistance.

This work was supported by a grant from UNDP/World Bank/WHO-TDR and Lim-38 (Laboratórios de Investigação Científica do Hospital das Clínicas, FMUSP).

Recebido para publicação em 10/06/1991.

Aceito para publicação em 22/08/1991.

1. AFCHAIN, D. - Le caractére antigénique des Tripanosomatidae héteroxenes parasites de l' homme: Trypanosoma (S) cruzi, Trypanosoma (T) gambiense et Leishmania donovani Lille, 1976. (Ph.D thesis, Université des Sciences et Techniques de Lille).
2. CAMARGO, M.E. & REBONATO, C. - Cross reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies. A simple inhibition procedure to ensure specific results. Amer. J. trop. Med. Hyg., 18: 500-505, 1969.
3. CELESTE, B.J. & GUIMARĂES, M.C.S. - Growth curves of Leishmania braziliensis braziliensis promastigotes and surface antigen expression before and after adaptation to Schneider's Drosophila medium as assessed by anti-Leishmanis human sera. Rev. Inst. Med. trop. S. Paulo, 30: 63-67, 1988.
4. CHILDS, G.E.; FOSTER, K.A. & McROBERTS, M.J. - Insect cell culture media for cultivation of New World Leishmania. Ann. Trop. Med. Parasit., 73: 395-396, 1970.
5. CUBA CUBA, C.A.; MARSDEN, P.D.; BARRETO, A.C.; ROCHA, R.; SAMPAIO, R.R. & PATZLAFF, L. - Diagnostico parasitologico e inmunologico de leishmaniasis tegumentaria americana. Bol. Ofic. sanit. panamer., 89: 195-208, 1980.
6. FERNANDES, J.F. & CASTELLANI, O. - Growth characteristics and chemical composition of Trypanosoma cruzi. Exp. Parasit., 18: 195-202, 1966.
7. FLEISS, J.L. - Statistical methods for rates and proportions. New York, John Wiley and Sons, 1973.
8. GUIMARĂES, M.C.S.; GIOVANNINI, V.L. & CAMARGO, M.E. - Antigenic standardization for muco-cutaneous leishmaniasis immunofluorescence test. Rev. Inst. Med. trop. S. Paulo, 16: 145-148, 1974.
9. GUIMARĂES, M.C.S.; CELESTE, B.J.; QUARTIER, V. de P. & FRANCO, E.F. - Soroepidemiologia da leishmaniose mucocutânea. Concometância de anticorpos anti-p. falciparum In: Congresso da Sociedade Brasileira de Medicina Tropical, 22, Belo Horizonte, M.G., 1986. Resumos. p. 78. (Rev. Soc. bras. Med. trop., 19 (Supl.), 1986).
10. GUIMARĂES, M.C.S.; CELESTE, B.J.; FRANCO, E.F.; CUCÉ, L.C. & BELDA JR., W. - Evaluation of serological diagnostic indices for mucocutaneous leishmaniasis: immunofluorescence tests and enzume-linked immunoassays for IgG, IgM and IgA antibodies. Bull. WHO, 67: 643-648, 1989.
11. JAFFE, C.L.; GRIMALDI, G. & McMAHON-PRATT, D. - The cultivation and cloning of Leishmania In: Morel, C.M., ed. Genes and antigens of parasites. A laboratory manual. 2nd. ed. Rio de Janeiro, Fundaçăo Oswaldo Cruz. p. 47-92, 1984.
12. MARZOCHI, M.C. de A.; COUTINHO, S.G.; SABROZA, P.C. & SOUZA, W.J.S. - Reaçăo de imunofluorescęncia indireta e intradermorreaçăo para leishmaniose tegumentar americana em moradores na área de Jacarepaguá (Rio de Janeiro). Estudo comparativo dos resultados observados em 1974 e 1978. Rev. Inst. Med. trop. S. Paulo, 22: 149-155, 1980.
13. MOMEN, H.; GRIMALDI JR., G.; PACHECO, R.S.; JAFFE, C.L.; McMAHON-PRATT, D. & MARZOCHI, M.C.A. - Brazilian Leishmania stocks phenotypically similar to Leishmania major. Amer. J. trop. Med. Hyg., 34: 1076-1084, 1985.
14. MONTENEGRO, J. - A cutis reaçăo na leishmaniose. An. Fac. Med. S. Paulo, 1: 323-330, 1926.
15. NOVY, F.G. & McNEAL, W.J. - On the cultivation of Trypanosoma brucei. J. infect. Dis., 1: 1-30, 1904.
16. PAPPAS, M.J.; McGREEVY, P.B.; HAJKOWSKI, R.; HENDRICKS, L.D.; OSTER, C.N. & HOCKMEYER, W.T. - Evaluation of promastigote and amastigote antigens in the indirect fluorescent antibody test for American cutaneous leshmaniasis. Amer. J. trop. Med. Hyg., 32: 1260-1267, 1983.

Address for correspondence:

Dr. Maria Carolina S. Guimarães

Laboratório de Soroepidemiologia, Instituto de Medicina Tropical de S. Paulo

Av. Dr. Eneas de Carvalho Aguiar, 470

05403, S. Paulo, Brazil

Publication Dates

Publication in this collection
11 Sept 2006
Date of issue
Dec 1991

History

Accepted
22 Aug 1991
Received
10 June 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. AFCHAIN, D. - Le caractére antigénique des Tripanosomatidae héteroxenes parasites de l' homme: Trypanosoma (S) cruzi, Trypanosoma (T) gambiense et Leishmania donovani Lille, 1976. (Ph.D thesis, Université des Sciences et Techniques de Lille).

[2] 2. CAMARGO, M.E. & REBONATO, C. - Cross reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies. A simple inhibition procedure to ensure specific results. Amer. J. trop. Med. Hyg., 18: 500-505, 1969.

[3] 3. CELESTE, B.J. & GUIMARĂES, M.C.S. - Growth curves of Leishmania braziliensis braziliensis promastigotes and surface antigen expression before and after adaptation to Schneider's Drosophila medium as assessed by anti-Leishmanis human sera. Rev. Inst. Med. trop. S. Paulo, 30: 63-67, 1988.

[4] 4. CHILDS, G.E.; FOSTER, K.A. & McROBERTS, M.J. - Insect cell culture media for cultivation of New World Leishmania. Ann. Trop. Med. Parasit., 73: 395-396, 1970.

[5] 5. CUBA CUBA, C.A.; MARSDEN, P.D.; BARRETO, A.C.; ROCHA, R.; SAMPAIO, R.R. & PATZLAFF, L. - Diagnostico parasitologico e inmunologico de leishmaniasis tegumentaria americana. Bol. Ofic. sanit. panamer., 89: 195-208, 1980.

[6] 6. FERNANDES, J.F. & CASTELLANI, O. - Growth characteristics and chemical composition of Trypanosoma cruzi. Exp. Parasit., 18: 195-202, 1966.

[7] 7. FLEISS, J.L. - Statistical methods for rates and proportions. New York, John Wiley and Sons, 1973.

[8] 8. GUIMARĂES, M.C.S.; GIOVANNINI, V.L. & CAMARGO, M.E. - Antigenic standardization for muco-cutaneous leishmaniasis immunofluorescence test. Rev. Inst. Med. trop. S. Paulo, 16: 145-148, 1974.

[9] 9. GUIMARĂES, M.C.S.; CELESTE, B.J.; QUARTIER, V. de P. & FRANCO, E.F. - Soroepidemiologia da leishmaniose mucocutânea. Concometância de anticorpos anti-p. falciparum In: Congresso da Sociedade Brasileira de Medicina Tropical, 22, Belo Horizonte, M.G., 1986. Resumos. p. 78. (Rev. Soc. bras. Med. trop., 19 (Supl.), 1986).

[10] 10. GUIMARĂES, M.C.S.; CELESTE, B.J.; FRANCO, E.F.; CUCÉ, L.C. & BELDA JR., W. - Evaluation of serological diagnostic indices for mucocutaneous leishmaniasis: immunofluorescence tests and enzume-linked immunoassays for IgG, IgM and IgA antibodies. Bull. WHO, 67: 643-648, 1989.

[11] 11. JAFFE, C.L.; GRIMALDI, G. & McMAHON-PRATT, D. - The cultivation and cloning of Leishmania In: Morel, C.M., ed. Genes and antigens of parasites. A laboratory manual. 2nd. ed. Rio de Janeiro, Fundaçăo Oswaldo Cruz. p. 47-92, 1984.

[12] 12. MARZOCHI, M.C. de A.; COUTINHO, S.G.; SABROZA, P.C. & SOUZA, W.J.S. - Reaçăo de imunofluorescęncia indireta e intradermorreaçăo para leishmaniose tegumentar americana em moradores na área de Jacarepaguá (Rio de Janeiro). Estudo comparativo dos resultados observados em 1974 e 1978. Rev. Inst. Med. trop. S. Paulo, 22: 149-155, 1980.

[13] 13. MOMEN, H.; GRIMALDI JR., G.; PACHECO, R.S.; JAFFE, C.L.; McMAHON-PRATT, D. & MARZOCHI, M.C.A. - Brazilian Leishmania stocks phenotypically similar to Leishmania major. Amer. J. trop. Med. Hyg., 34: 1076-1084, 1985.

[14] 14. MONTENEGRO, J. - A cutis reaçăo na leishmaniose. An. Fac. Med. S. Paulo, 1: 323-330, 1926.

[15] 15. NOVY, F.G. & McNEAL, W.J. - On the cultivation of Trypanosoma brucei. J. infect. Dis., 1: 1-30, 1904.

[16] 16. PAPPAS, M.J.; McGREEVY, P.B.; HAJKOWSKI, R.; HENDRICKS, L.D.; OSTER, C.N. & HOCKMEYER, W.T. - Evaluation of promastigote and amastigote antigens in the indirect fluorescent antibody test for American cutaneous leshmaniasis. Amer. J. trop. Med. Hyg., 32: 1260-1267, 1983.

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