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Revista do Instituto de Medicina Tropical de São Paulo

Print version ISSN 0036-4665

Rev. Inst. Med. trop. S. Paulo vol.34 no.1 São Paulo Jan./Feb. 1992

http://dx.doi.org/10.1590/S0036-46651992000100004 

ORIGINAL ARTICLES

 

Comparative study of adenoviruses with monoclonal antibodies

 

Estudo comparativo de diferentes tipos de adenovirus através de anticorpos monoclonais

 

 

Terezinha Maria de PaivaI; Sueko TakimotoI; María Akíko IshidaI; María Candida Oliveira de SouzaI; Tuneo IshimaruI; Jorge NeumannII; Jorge KalilII

ILaboratório de Vírus Respiratórios do Instituto Adolfo Lutz
IILaboratório de Imunologia de Transplantes da Faculdade de Medicina da Universidade de São Paulo

Endereço para correspondência

 

 


SUMMARY

The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D).
Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained.

Key words: Adenovirus, Species Specific, Monoclonal antibodies, Adenovirus 4, Antigenic Relationship.


RESUMO

O estudo relata a obtenção de anticorpos monoclonais para o adenovirus tipo (espécie) 4. A especificidade dos anticorpos monoclonais, selecionados nesse experimento, foi determinada testando-os frente às diferentes espécies de adenovírus: 1, 2, 5 (subgênero C), 4 (subgênero E), 7 e 16 (subgênero B) e 9 (subgênero D), pelas técnicas de neutralização em cultura de células, imunofluorescência e ensaio imunoenzimático (ELISA).
Os resultados demonstram a obtenção de anticorpos monoclonais que reagiram de maneira específica para o adenovírus 4 (1CIII e 3DIII) e anticorpo monoclonal apresentando reação cruzada com as espécies de adenovírus 4 e 7 (2HIII).


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 

 

ACKNOWLEDGEMENTS

We would like to express our gratefulness to Dr. Luiz Florencio de Salles Gomes, Dr. Eliseu Alves Waldman, Dra. Clélia Helena de Oliveira Martinez, Dr. Otávio Francisco Pinheiro de Oliva, Dra. Jussara Nascimento, the personel of the Department of Photograph of the Instituto Adolfo Lutz and the Department of Rheumatology of the Faculdade de Medicina da Universidade de São Paulo.

 

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Endereço para correspondência:
Terezinha Maria de Paiva
Laboratório de Vírus Respiratórios, Instituto Adolfo Lutz
Av. Dr. Arnaldo nº 355
CEP: 01246 - São Paulo, SP. Brasil

Recebido para publicação em 25/2/1991
Aceito para publicação em 24/10/1991