Comparative study of adenoviruses with monoclonal antibodies

Paiva, Terezinha Maria de; Takimoto, Sueko; Ishida, María Akíko; Souza, María Candida Oliveira de; Ishimaru, Tuneo; Neumann, Jorge; Kalil, Jorge

doi:10.1590/S0036-46651992000100004

Abstracts

The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D). Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained.

Adenovirus; Species Specific; Monoclonal antibodies; Adenovirus 4; Antigenic Relationship

O estudo relata a obtenção de anticorpos monoclonais para o adenovirus tipo (espécie) 4. A especificidade dos anticorpos monoclonais, selecionados nesse experimento, foi determinada testando-os frente às diferentes espécies de adenovírus: 1, 2, 5 (subgênero C), 4 (subgênero E), 7 e 16 (subgênero B) e 9 (subgênero D), pelas técnicas de neutralização em cultura de células, imunofluorescência e ensaio imunoenzimático (ELISA). Os resultados demonstram a obtenção de anticorpos monoclonais que reagiram de maneira específica para o adenovírus 4 (1CIII e 3DIII) e anticorpo monoclonal apresentando reação cruzada com as espécies de adenovírus 4 e 7 (2HIII).

ORIGINAL ARTICLES

Comparative study of adenoviruses with monoclonal antibodies

Estudo comparativo de diferentes tipos de adenovirus através de anticorpos monoclonais

Terezinha Maria de Paiva^I; Sueko Takimoto^I; María Akíko Ishida^I; María Candida Oliveira de Souza^I; Tuneo Ishimaru^I; Jorge Neumann^II; Jorge Kalil^II

^ILaboratório de Vírus Respiratórios do Instituto Adolfo Lutz

^IILaboratório de Imunologia de Transplantes da Faculdade de Medicina da Universidade de São Paulo

^{Endereço para correspondência} Endereço para correspondência: Terezinha Maria de Paiva Laboratório de Vírus Respiratórios, Instituto Adolfo Lutz Av. Dr. Arnaldo nº 355 CEP: 01246 - São Paulo, SP. Brasil

SUMMARY

The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D).

Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained.

Key words: Adenovirus, Species Specific, Monoclonal antibodies, Adenovirus 4, Antigenic Relationship.

RESUMO

O estudo relata a obtenção de anticorpos monoclonais para o adenovirus tipo (espécie) 4. A especificidade dos anticorpos monoclonais, selecionados nesse experimento, foi determinada testando-os frente às diferentes espécies de adenovírus: 1, 2, 5 (subgênero C), 4 (subgênero E), 7 e 16 (subgênero B) e 9 (subgênero D), pelas técnicas de neutralização em cultura de células, imunofluorescência e ensaio imunoenzimático (ELISA).

Os resultados demonstram a obtenção de anticorpos monoclonais que reagiram de maneira específica para o adenovírus 4 (1CIII e 3DIII) e anticorpo monoclonal apresentando reação cruzada com as espécies de adenovírus 4 e 7 (2HIII).

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENTS

We would like to express our gratefulness to Dr. Luiz Florencio de Salles Gomes, Dr. Eliseu Alves Waldman, Dra. Clélia Helena de Oliveira Martinez, Dr. Otávio Francisco Pinheiro de Oliva, Dra. Jussara Nascimento, the personel of the Department of Photograph of the Instituto Adolfo Lutz and the Department of Rheumatology of the Faculdade de Medicina da Universidade de São Paulo.

Recebido para publicação em 25/2/1991

Aceito para publicação em 24/10/1991

01. ADAM, E.; LENGYEL, A.; KERÉKGYÂRTÓ, C.S.; ERDEY, J.; FACHET, J. & NÁSZ, I. - Characterization of monoclonal antibodies to adenovirus type 35 hexon. Acta virol., 32: 393-402, 1988.
02. BRADFORD, M.M. - A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analyt. Biochem., 72: 248-254, 1976.
03. DUDDING, B.A.; WAGNER, S.C. & ZELLER, J.A. - Fatal pneumonia associated with adenovirus 7 in three military trainess. New Engl. J. Med., 286: 1289-1292, 1972.
04. GARON, C.F.; BERRY, K.W.; HIERHOLZER, J.C. & ROSE, J.A. - Mapping of base sequence heterologies between genomes from different adenovirus serotypes. Virology, 54: 414-426, 1973.
05. GREEN, M. - Oncogenic viruses. - Ann. Rev. Biochem., 39: 701-756, 1970.
06. GREEN, M.; MACKEY, J.K.; WOLD, W.S.M. & RIDGEN, P. Thirty one human adenovirus serotypes (ad 1 - ad 31) from five groups (A-E) based upon DNA genome homologies. Virology, 93: 481-492, 1979.
07. HERRMANN, J.E.; PERRON-HENRY, D.M.; STOBBS-WALRO, D. & BLACKLOW, N.R. - Preparation and characterization of monoclonal antibodies to Enteric Adenovirus Types 40 and 41. Arch. Virol., 94: 3-4, 1987.
08. HIERHOLZER, J.C. - Further subgrouping of the human adenovirus by differential hemagglutination. J. infect. Dis., 128: 541-550, 1973.
09. HIERHOLZER, J.C.; WIGAND, R.; ANDERSON, L.J.; ADRIAN, T. & GOLD, J.W.M. - Adenovirus from patients with AIDS: A Plethora of serotypes and a description of five new serotypes of subgenus D (types 43-47). J. infect. Dis., 158: 804-813, 1988.
10. HUEBNER, R.J. - Adenovirus directed tumor and T antigens. In: POLLARD, M., ed. Perpectives in virology. New York, Academic Press, 1967. v. 5, p. 147-166.
11. LU, J.R.; FU, W.Y.; CHENG, H.J.; CHEN, S.L.; GUO, H.J.; LENG, L.; WU, Y.M.; CHEN, Y.Y. & QI, Y.M. - Identification of the types of adenovirus isolates with monoclonal antibodies. Chin. med. J.; 102(8): 650-654, 1989.
12. Mc ALLISTER, R.M.; NICOLSON, M.O.; REED, G.; KERN, J.L.; GILDEN, R.V. & HUEBNER, R.J. - Transformation of rodent cells by adenovirus 19 and other group D adenoviruses. J. nat. Cancer Inst., 43: 917-923, 1969.
13. NORRBY, E. & WADELL, G. - Immunological relationships between hexons of certain human adenoviruses. J. Virol., 4: 663-670, 1969.
14. PETTERSON, V. - Structural proteins of adenoviruses VI. On the antigenic determinants of the hexon. Virology, 43: 123-136, 1971.
15. PHILIPSON, L.; PETTERSON, V & LINDBERG, V. - Molecular biology of adenoviruses. In: GARD, S. & HALLAUER, C. Virology Monograph, New York, Springer - Verlag, 1975. Vol. 14, p 1-115
16. PINA, M. & GREEN, M. - Biochemical studies on adenovirus multiplication, IX. Chemical and base composition analysis of 28 human adenoviruses. Proc. Nat. Acad. Sci. (Wash.), 54: 547-551, 1965.
17. PRICE, J.P. - Hybridoma Technology. Adv. Cell. Cult., 4: 157-177, 1985.
18. ROSEN, L. - A hemagglutination inhibition technique for typing adenoviruses. Amer. J. Hyg., 71: 120-128, 1960.
19. ROWE, W.P.; HUEBNER, R.J.; GILMORE, L.K.; PARROT, R.M. & WARD, T.G. - Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. exp. Biol. Med., 84: 570-573, 1953.
20. TOKUNNAGA, O.; YAEGASHI, T.; LOWE, J.; DOBBS, L. & PADMANABHAN, R. - Sequence analysis in the E1 region of adenovirus type 4 DNA. Virology, 155: 418-433, 1986.
21. VOLLER, A.; BIDWELL, D.E. & BARTLETT, A. - Enzyme immunoassays in diagnostic medicine. Bull. Wld. Hlth. Org., 53: 55-65, 1976.
22. WADELL, G. & NORRBY, E. - Immunological and other biological characteristics of pentons of human adenovirus. J. Virol., 4: 671-680, 1969.
23. WADELL, G. - Classification of human adenovirus by SDS polyacrylamide gel electrophoresis of structural polypeptides. Intervirology, 11: 47-57, 1979.
24. WIGAND, R.; BARTHA, A.; DREIZIN, R.S.; ESCHE, H.; GINSBERG, H.S.; GREEN, M.; HIERHOLZER, J.C.; KALTER, S.S.; McFERRAN, J.B.; PETTERSON, U.; RUSSEL, W.C. & WADELL, G. - Adenoviridae: second report. Intervirology, 18: 169-176, 1982.
25. WILCOX, N. & MAUTNER, V. - Antigenic determinants of adenovirus capsids II. Homogeneity of hexons, and accessibility of their determinants in the virion. J. Immunol., 116: 25-29, 1976.

Endereço para correspondência:

Terezinha Maria de Paiva

Laboratório de Vírus Respiratórios, Instituto Adolfo Lutz

Av. Dr. Arnaldo nº 355

CEP: 01246 - São Paulo, SP. Brasil

Publication Dates

Publication in this collection
06 Sept 2006
Date of issue
Feb 1992

History

Received
25 Feb 1991
Accepted
24 Oct 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 01. ADAM, E.; LENGYEL, A.; KERÉKGYÂRTÓ, C.S.; ERDEY, J.; FACHET, J. & NÁSZ, I. - Characterization of monoclonal antibodies to adenovirus type 35 hexon. Acta virol., 32: 393-402, 1988.

[2] 02. BRADFORD, M.M. - A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analyt. Biochem., 72: 248-254, 1976.

[3] 03. DUDDING, B.A.; WAGNER, S.C. & ZELLER, J.A. - Fatal pneumonia associated with adenovirus 7 in three military trainess. New Engl. J. Med., 286: 1289-1292, 1972.

[4] 04. GARON, C.F.; BERRY, K.W.; HIERHOLZER, J.C. & ROSE, J.A. - Mapping of base sequence heterologies between genomes from different adenovirus serotypes. Virology, 54: 414-426, 1973.

[5] 05. GREEN, M. - Oncogenic viruses. - Ann. Rev. Biochem., 39: 701-756, 1970.

[6] 06. GREEN, M.; MACKEY, J.K.; WOLD, W.S.M. & RIDGEN, P. Thirty one human adenovirus serotypes (ad 1 - ad 31) from five groups (A-E) based upon DNA genome homologies. Virology, 93: 481-492, 1979.

[7] 07. HERRMANN, J.E.; PERRON-HENRY, D.M.; STOBBS-WALRO, D. & BLACKLOW, N.R. - Preparation and characterization of monoclonal antibodies to Enteric Adenovirus Types 40 and 41. Arch. Virol., 94: 3-4, 1987.

[8] 08. HIERHOLZER, J.C. - Further subgrouping of the human adenovirus by differential hemagglutination. J. infect. Dis., 128: 541-550, 1973.

[9] 09. HIERHOLZER, J.C.; WIGAND, R.; ANDERSON, L.J.; ADRIAN, T. & GOLD, J.W.M. - Adenovirus from patients with AIDS: A Plethora of serotypes and a description of five new serotypes of subgenus D (types 43-47). J. infect. Dis., 158: 804-813, 1988.

[10] 10. HUEBNER, R.J. - Adenovirus directed tumor and T antigens. In: POLLARD, M., ed. Perpectives in virology. New York, Academic Press, 1967. v. 5, p. 147-166.

[11] 11. LU, J.R.; FU, W.Y.; CHENG, H.J.; CHEN, S.L.; GUO, H.J.; LENG, L.; WU, Y.M.; CHEN, Y.Y. & QI, Y.M. - Identification of the types of adenovirus isolates with monoclonal antibodies. Chin. med. J.; 102(8): 650-654, 1989.

[12] 12. Mc ALLISTER, R.M.; NICOLSON, M.O.; REED, G.; KERN, J.L.; GILDEN, R.V. & HUEBNER, R.J. - Transformation of rodent cells by adenovirus 19 and other group D adenoviruses. J. nat. Cancer Inst., 43: 917-923, 1969.

[13] 13. NORRBY, E. & WADELL, G. - Immunological relationships between hexons of certain human adenoviruses. J. Virol., 4: 663-670, 1969.

[14] 14. PETTERSON, V. - Structural proteins of adenoviruses VI. On the antigenic determinants of the hexon. Virology, 43: 123-136, 1971.

[15] 15. PHILIPSON, L.; PETTERSON, V & LINDBERG, V. - Molecular biology of adenoviruses. In: GARD, S. & HALLAUER, C. Virology Monograph, New York, Springer - Verlag, 1975. Vol. 14, p 1-115

[16] 16. PINA, M. & GREEN, M. - Biochemical studies on adenovirus multiplication, IX. Chemical and base composition analysis of 28 human adenoviruses. Proc. Nat. Acad. Sci. (Wash.), 54: 547-551, 1965.

[17] 17. PRICE, J.P. - Hybridoma Technology. Adv. Cell. Cult., 4: 157-177, 1985.

[18] 18. ROSEN, L. - A hemagglutination inhibition technique for typing adenoviruses. Amer. J. Hyg., 71: 120-128, 1960.

[19] 19. ROWE, W.P.; HUEBNER, R.J.; GILMORE, L.K.; PARROT, R.M. & WARD, T.G. - Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. exp. Biol. Med., 84: 570-573, 1953.

[20] 20. TOKUNNAGA, O.; YAEGASHI, T.; LOWE, J.; DOBBS, L. & PADMANABHAN, R. - Sequence analysis in the E1 region of adenovirus type 4 DNA. Virology, 155: 418-433, 1986.

[21] 21. VOLLER, A.; BIDWELL, D.E. & BARTLETT, A. - Enzyme immunoassays in diagnostic medicine. Bull. Wld. Hlth. Org., 53: 55-65, 1976.

[22] 22. WADELL, G. & NORRBY, E. - Immunological and other biological characteristics of pentons of human adenovirus. J. Virol., 4: 671-680, 1969.

[23] 23. WADELL, G. - Classification of human adenovirus by SDS polyacrylamide gel electrophoresis of structural polypeptides. Intervirology, 11: 47-57, 1979.

[24] 24. WIGAND, R.; BARTHA, A.; DREIZIN, R.S.; ESCHE, H.; GINSBERG, H.S.; GREEN, M.; HIERHOLZER, J.C.; KALTER, S.S.; McFERRAN, J.B.; PETTERSON, U.; RUSSEL, W.C. & WADELL, G. - Adenoviridae: second report. Intervirology, 18: 169-176, 1982.

[25] 25. WILCOX, N. & MAUTNER, V. - Antigenic determinants of adenovirus capsids II. Homogeneity of hexons, and accessibility of their determinants in the virion. J. Immunol., 116: 25-29, 1976.