Víbrios não coléricos na rotina enterobacteriológica

Magalhães, Vera; Magalhães, Marcelo; Lima, Roberto A.; Tateno, Seiki; Magalhães, Eduardo

doi:10.1590/S0036-46651992000200008

Resumos

De 3.250 fezes diarréicas, recebidas para diagnóstico microbiológico em laboratório clínico particular, no Recife, Brasil, isolaram-se 55 (1,7%) linhagens de Vibrio. O estudo foi realizado entre maio de 1989 e maio de 1991. Para o isolamento de Vibrio, os espécimes fecais foram enriquecidos em água peptonada alcalina suplementada com 2% de NaCl e subcultivados em ágar tiosulfato-citrato-sais biliares-sacarose (TCBS). Das espécies isoladas, V. parahaemolyticus foi a mais freqüente (24 cepas), seguida de V. furnissii (15 cepas), V. cholerae não 01 (6 cepas), V. alginolyticus (4 cepas), V. fluvialis (2 cepas) e Vibrio sp. (1 cepa). Do ponto de vista custo-benefício, a baixa taxa de isolamento de Vibrio levanta dúvidas acerca da utilidade do TCBS na rotina enterobacteriológica dos laboratórios clínicos

Vibrios; Diarréia; Coprocultura

Of 3250 diarrheal stools received for microbiologic diagnosis at a private clinical laboratory in Recife, Brazil, strains of Vibrio were isolated from 55 (1.7%). The study was carried out from May 1989 through May 1991. For recovering Vibrio, fecal samples were enriched in alkaline peptone water supplemented with 2% NaCl and subcultured on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Of the recovered species, V. parahaemolyticus was most commonly found (24 strains), followed by V. furnissii (15 strains), V. cholerae non-01 (6 strains), V. alginolyticus (4 strains), V. fluvialis (2 strains), and Vibrio sp. (1 strain). The low isolation rate of Vibrio raises doubts about the cost-effectiveness of the use of TCBS in the routine enterobacteriologic workup of clinical laboratories

ARTIGOS ORIGINAIS

Víbrios não coléricos na rotina enterobacteriológica

Non-cholera vibrios in enterobacteriologic routine

Vera Magalhães^I; Marcelo Magalhães^II; Roberto A. Lima^III; Seiki Tateno^II; Eduardo Magalhães^IV

^IDepartamento de Medicina Tropical, CCS/UFPE. Recife, PE, Brasil

^IILaboratório Keizo Asami, UFPE. Recife, PE, Brasil

^IIIDepartamento de Medicina Clínica, CCS/UFPE. Recife, PE, Brasil

^IVCentro de Microbiologia e Imunologia. Recife, PE, Brasil

^{Endereço para correspondência} Endereço para correspondência: Marcelo Magalhães Laboratório Keizo Asami, Universidade Federal de Pernambuco Cidade Universitária Recife, 50730, Brasil

RESUMO

De 3.250 fezes diarréicas, recebidas para diagnóstico microbiológico em laboratório clínico particular, no Recife, Brasil, isolaram-se 55 (1,7%) linhagens de Vibrio. O estudo foi realizado entre maio de 1989 e maio de 1991. Para o isolamento de Vibrio, os espécimes fecais foram enriquecidos em água peptonada alcalina suplementada com 2% de NaCl e subcultivados em ágar tiosulfato-citrato-sais biliares-sacarose (TCBS). Das espécies isoladas, V. parahaemolyticus foi a mais freqüente (24 cepas), seguida de V. furnissii (15 cepas), V. cholerae não 01 (6 cepas), V. alginolyticus (4 cepas), V. fluvialis (2 cepas) e Vibrio sp. (1 cepa). Do ponto de vista custo-benefício, a baixa taxa de isolamento de Vibrio levanta dúvidas acerca da utilidade do TCBS na rotina enterobacteriológica dos laboratórios clínicos.

Unitermos: Vibrios; Diarréia; Coprocultura.

SUMMARY

Of 3250 diarrheal stools received for microbiologic diagnosis at a private clinical laboratory in Recife, Brazil, strains of Vibrio were isolated from 55 (1.7%). The study was carried out from May 1989 through May 1991. For recovering Vibrio, fecal samples were enriched in alkaline peptone water supplemented with 2% NaCl and subcultured on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Of the recovered species, V. parahaemolyticus was most commonly found (24 strains), followed by V. furnissii (15 strains), V. cholerae non-01 (6 strains), V. alginolyticus (4 strains), V. fluvialis (2 strains), and Vibrio sp. (1 strain). The low isolation rate of Vibrio raises doubts about the cost-effectiveness of the use of TCBS in the routine enterobacteriologic workup of clinical laboratories.

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Recebido para publicação em 22/8/1991.

Aceito para publicação em 27/12/1991.

Trabalho financiado pela Japan International Cooperation Agency (JICA).

1. BARKER Jr., W.H. - Vibrio parahaemolyticus outbreaks in the United States. Lancet, 1: 551-554,1974.
2. BARKER Jr., W.H.; WEAVER, R.E.; MORRIS, G.K. & MARTIN, W.T. - Epidemiology of Vibrio parahaemolyticus infection in humans. In: SCHLESSINGER, D. ed. Microbiology - 1974. Washington, American Society for Microbiology, 1975. p. 257-262.
3. BAUMANN, P.; FURNISS, A.L. & LEE, J.V. - Genus I. Vibrio Pacine 854.In: KRIEG, N.R. & HOLT, J.G., ed. Bergey's Manual of Systematic Bacteriology. Baltimore, Williams and Wilkins, 1984. p. 518-538.
4. BONNER, J.R.; COKER, A.S.; BERRYMAN, C.R. & POLLOC, H.M. - Spectrum of Vibrio infections in a Gulf coast community. Ann. intern. Med., 99: 464-469,1983.
5. CENTER FOR DISEASE CONTROL - An outbreak of acute gastroenteritis during a tour of the Orient-Alaska. M.M.W.R., 18: 150,1969.
6. COWELL, R.R. - Occurrence and biology of Vibrio parahaemolyticus. In: SCHLESSINGER, D., ed. Microbiology - 1974. Washington, Amercian Society for Microbiology, 1975. p. 230-240.
7. FARMER III, J.J.; HICKMAN-BRENNER, F.W. & KELLY, M.T. - Vibrio. In: LENNETTE, E.H.; BALOWS, A.; HAUSLER Jr., W.J. & SHADOMY, H.J., ed. Manual of Clinical Microbiology. 4. ed. Washington, American Socitey for Microbiology, 1985. p. 282-301.
8. FUJINO, T.; OKUNO, Y.; NAKADA, D.; AOYAMA, A.; FUKAI, K.; MUKAI, T. & UEHO, T. - On the bacteriological examination of shirasu food poisoning. Med. J. Osaka Univ., 4: 299-304,1953.
9. FUJINO, T.; SAKAZAKI, R. & TAMURA, K. - Designation of the type strain of Vibrio parahaemolyticus and description of 200 strains of the species. Int. J. system. Bact., 24: 447-449,1974.
10. HOFER, E. - Primeiro isolamento e identificaçăo de Vibrio parahaemolyticus no Brasil de infecçăo gastrointestinal humana. Rev. Microbiol. (S. Paulo), 14: 174-175, 1983.
11. HOFER, E. - Vibrio cholerae năo 01 associado a infecçăo entérica humana no estado da Bahia, Brasil. Rev. Microbiol. (S. Paulo), 18: 1-4, 1987.
12. HOGE, C.W.; WATSKY, D.; PEELER, R.N.; LIBONATI, J.P.; ISRAEL, E. & MORRIS Jr., J.G. - Epidemiology and spectrum of vibrio infections in a Chesapeake Bay community. J. infect. Dis., 160: 985-993, 1989.
13. HUGHES, J.M.; HOLUS, D.G.; GANGAROSA, E.J. & WEAVER, R.E. - Non-cholera vibrio infections in the United States. Ann. intern. Med., 88: 602-606, 1978.
14. HUQ, M.I.; ALAM, A.K.M.J.; BRENNER, D.J. & MORRIS, G.K. - Isolation of Vibrio -like group, EF-6, from patients with diarrhea. J. clin. Microbiol, 11: 621-624, 1980.
15. JANDA, J.M.; POWERS, C.; BRYANT, R.G. & ABBOTT, S.L. - Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin. Microbiol. Rev., 1: 245-267,1988.
16. KELLY, M.T.; HICKMAN-BRENNER, F.W. & FARMER III, J.J. - Vibrio. In: BALOWS, A.; HAUSLER Jr., W.J.; HERRMANN, K.L.; ISENBERG, H.D. & SHADOMY, H.J., ed. Manual of Clinical Microbiology. 5. ed. Washington, American Society for Microbiology, 1991. p. 384-395.
17. LEE, J.V.; SHREAD, P.; FURNISS, A.L. & BRYANT, T.N. - Taxonomy and description of Vibrio fluvialis sp. nov. (synonym group F vibrios, group EF6). J. appl. Bact, 50: 73-94,1981.
18. LENNETTE, E.H.; BALOWS, A.; HAUSLER Jr., W.J. & SHADOMY, H.J. - Manual of Clinical Microbiology. 4. ed. Washington, American Society for Microbiology, 1985.
19. MAGALHĂES, M.; SILVA, G.P da; MAGALHĂES, V.; ANTAS, M.G.; ANDRADE, M.A. & TATENO, S. - Vibrio fluvialis and Vibrio furnissii associated with infantile diarrhea. Rev. Microbiol. (S. Paulo), 21: 295-298, 1990.
20. MAGALHĂES, V.; LIMA, R.A.; MAGALHĂES, E. & MAGALHĂES, M. - Gastrenterite humana associada a Vibrio fluvialis no Recife. Arq. Gastroent. (S. Paulo), 27: 141-143,1990.
21. MAGALHĂES, V.; LIMA, A.R.; TATENO, S. & MAGALHĂES, M. - Gastroenterites humanas associadas a Vibrio parahaemolyticus no Recife, Brasil. Rev. Inst. Med. trop. S. Paulo, 33: 64-68, 1991.
22. MORRIS Jr., J.G. & BLACK, R.E. - Cholera and other vibriosis in the United States. New. Engl. J. Med., 312: 343-350,1985.

Endereço para correspondência:

Marcelo Magalhães

Laboratório Keizo Asami, Universidade Federal de Pernambuco

Cidade Universitária

Recife, 50730, Brasil

Datas de Publicação

Publicação nesta coleção
14 Set 2006
Data do Fascículo
Abr 1992

Histórico

Recebido
22 Ago 1991
Aceito
27 Dez 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BARKER Jr., W.H. - Vibrio parahaemolyticus outbreaks in the United States. Lancet, 1: 551-554,1974.

[2] 2. BARKER Jr., W.H.; WEAVER, R.E.; MORRIS, G.K. & MARTIN, W.T. - Epidemiology of Vibrio parahaemolyticus infection in humans. In: SCHLESSINGER, D. ed. Microbiology - 1974. Washington, American Society for Microbiology, 1975. p. 257-262.

[3] 3. BAUMANN, P.; FURNISS, A.L. & LEE, J.V. - Genus I. Vibrio Pacine 854.In: KRIEG, N.R. & HOLT, J.G., ed. Bergey's Manual of Systematic Bacteriology. Baltimore, Williams and Wilkins, 1984. p. 518-538.

[4] 4. BONNER, J.R.; COKER, A.S.; BERRYMAN, C.R. & POLLOC, H.M. - Spectrum of Vibrio infections in a Gulf coast community. Ann. intern. Med., 99: 464-469,1983.

[5] 5. CENTER FOR DISEASE CONTROL - An outbreak of acute gastroenteritis during a tour of the Orient-Alaska. M.M.W.R., 18: 150,1969.

[6] 6. COWELL, R.R. - Occurrence and biology of Vibrio parahaemolyticus. In: SCHLESSINGER, D., ed. Microbiology - 1974. Washington, Amercian Society for Microbiology, 1975. p. 230-240.

[7] 7. FARMER III, J.J.; HICKMAN-BRENNER, F.W. & KELLY, M.T. - Vibrio. In: LENNETTE, E.H.; BALOWS, A.; HAUSLER Jr., W.J. & SHADOMY, H.J., ed. Manual of Clinical Microbiology. 4. ed. Washington, American Socitey for Microbiology, 1985. p. 282-301.

[8] 8. FUJINO, T.; OKUNO, Y.; NAKADA, D.; AOYAMA, A.; FUKAI, K.; MUKAI, T. & UEHO, T. - On the bacteriological examination of shirasu food poisoning. Med. J. Osaka Univ., 4: 299-304,1953.

[9] 9. FUJINO, T.; SAKAZAKI, R. & TAMURA, K. - Designation of the type strain of Vibrio parahaemolyticus and description of 200 strains of the species. Int. J. system. Bact., 24: 447-449,1974.

[10] 10. HOFER, E. - Primeiro isolamento e identificaçăo de Vibrio parahaemolyticus no Brasil de infecçăo gastrointestinal humana. Rev. Microbiol. (S. Paulo), 14: 174-175, 1983.

[11] 11. HOFER, E. - Vibrio cholerae năo 01 associado a infecçăo entérica humana no estado da Bahia, Brasil. Rev. Microbiol. (S. Paulo), 18: 1-4, 1987.

[12] 12. HOGE, C.W.; WATSKY, D.; PEELER, R.N.; LIBONATI, J.P.; ISRAEL, E. & MORRIS Jr., J.G. - Epidemiology and spectrum of vibrio infections in a Chesapeake Bay community. J. infect. Dis., 160: 985-993, 1989.

[13] 13. HUGHES, J.M.; HOLUS, D.G.; GANGAROSA, E.J. & WEAVER, R.E. - Non-cholera vibrio infections in the United States. Ann. intern. Med., 88: 602-606, 1978.

[14] 14. HUQ, M.I.; ALAM, A.K.M.J.; BRENNER, D.J. & MORRIS, G.K. - Isolation of Vibrio -like group, EF-6, from patients with diarrhea. J. clin. Microbiol, 11: 621-624, 1980.

[15] 15. JANDA, J.M.; POWERS, C.; BRYANT, R.G. & ABBOTT, S.L. - Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin. Microbiol. Rev., 1: 245-267,1988.

[16] 16. KELLY, M.T.; HICKMAN-BRENNER, F.W. & FARMER III, J.J. - Vibrio. In: BALOWS, A.; HAUSLER Jr., W.J.; HERRMANN, K.L.; ISENBERG, H.D. & SHADOMY, H.J., ed. Manual of Clinical Microbiology. 5. ed. Washington, American Society for Microbiology, 1991. p. 384-395.

[17] 17. LEE, J.V.; SHREAD, P.; FURNISS, A.L. & BRYANT, T.N. - Taxonomy and description of Vibrio fluvialis sp. nov. (synonym group F vibrios, group EF6). J. appl. Bact, 50: 73-94,1981.

[18] 18. LENNETTE, E.H.; BALOWS, A.; HAUSLER Jr., W.J. & SHADOMY, H.J. - Manual of Clinical Microbiology. 4. ed. Washington, American Society for Microbiology, 1985.

[19] 19. MAGALHĂES, M.; SILVA, G.P da; MAGALHĂES, V.; ANTAS, M.G.; ANDRADE, M.A. & TATENO, S. - Vibrio fluvialis and Vibrio furnissii associated with infantile diarrhea. Rev. Microbiol. (S. Paulo), 21: 295-298, 1990.

[20] 20. MAGALHĂES, V.; LIMA, R.A.; MAGALHĂES, E. & MAGALHĂES, M. - Gastrenterite humana associada a Vibrio fluvialis no Recife. Arq. Gastroent. (S. Paulo), 27: 141-143,1990.

[21] 21. MAGALHĂES, V.; LIMA, A.R.; TATENO, S. & MAGALHĂES, M. - Gastroenterites humanas associadas a Vibrio parahaemolyticus no Recife, Brasil. Rev. Inst. Med. trop. S. Paulo, 33: 64-68, 1991.

[22] 22. MORRIS Jr., J.G. & BLACK, R.E. - Cholera and other vibriosis in the United States. New. Engl. J. Med., 312: 343-350,1985.