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A low cost method to produce a gaseous environment for the isolation of Helicobacter pylori

Un método de bajo costo para producir el ambiente gaseoso para el aislamiento de Helicobacter pylori

Abstracts

A low cost method (LCM) to produce a gaseous environment for the isolation of Helicobacter pylori, was compared with the standard Gas Park system. The LCM uses a carbonated antacid tablet, a plastic bag with tap water, a candle, and a wide-mouthed glass jar provided with a tight-fitting metalic screw cap and a rubber gasket. Antral gastric biopsies from 153 cases were incubated by duplicate on blood agar plates and treated with the two methods. In 95 cases the agent was isolated from both, and only from the standard method in 10 cases; the opposite condition was found in five cases, and 43 were negative. That difference is not significant (Pearson's X²= 93.25 p > 0,05)

Helicobacter pylori; Technical assay; Microaerobiosis


Se comparó un método de bajo costo (MBC) para producir el ambiente gaseoso para el aislamiento de Helicobacter pylori, con el sistema estándar de gas Pak. El MBC usa una tableta carbonata de antiácido, una bolsa plástica con agua, una candela y un frasco de vidrio con boca ancha, provisto de tapa metálica de rosca con empaque de hule. Las biopsias de antro de 153 pacientes se inocularon por duplicado en platos de agar sangre y se incubaron bajo los dos sistemas. En 195 casos el agente se aisló de ambos platos, y sólo del incubado bajo el método estándar en diez casos; la condición opuesta se presentó en cinco casos; 43 casos fueron negativos. Esa diferencia no es significativa (X² de Pearson = 93,25 p > 0,05)


TECHNICAL TRIALS

A low cost method to produce a gaseous environment for the isolation of Helicobacter pylori

Un método de bajo costo para producir el ambiente gaseoso para el aislamiento de Helicobacter pylori

Francisco HernándezI; Patricia RiveraII

IDepartamento de Microbiología, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica

IILaboratorio de Microbiología, Departamento de Anatomía Patológica, Hospital Nacional de Niños, San José, Costa Rica

SUMMARY

A low cost method (LCM) to produce a gaseous environment for the isolation of Helicobacter pylori, was compared with the standard Gas Park system. The LCM uses a carbonated antacid tablet, a plastic bag with tap water, a candle, and a wide-mouthed glass jar provided with a tight-fitting metalic screw cap and a rubber gasket. Antral gastric biopsies from 153 cases were incubated by duplicate on blood agar plates and treated with the two methods. In 95 cases the agent was isolated from both, and only from the standard method in 10 cases; the opposite condition was found in five cases, and 43 were negative. That difference is not significant (Pearson's X2= 93.25 p > 0,05).

Key words: Helicobacter pylori: Technical assay; Microaerobiosis.

RESUMEN

Se comparó un método de bajo costo (MBC) para producir el ambiente gaseoso para el aislamiento de Helicobacter pylori, con el sistema estándar de gas Pak. El MBC usa una tableta carbonata de antiácido, una bolsa plástica con agua, una candela y un frasco de vidrio con boca ancha, provisto de tapa metálica de rosca con empaque de hule. Las biopsias de antro de 153 pacientes se inocularon por duplicado en platos de agar sangre y se incubaron bajo los dos sistemas. En 195 casos el agente se aisló de ambos platos, y sólo del incubado bajo el método estándar en diez casos; la condición opuesta se presentó en cinco casos; 43 casos fueron negativos. Esa diferencia no es significativa (X2 de Pearson = 93,25 p > 0,05).

Full text available only in PDF format.

Texto completo disponible sólo en PDF.

ACKNOWLEDGEMENTS

We thanks Julian Monge-Nagera and Bernal Fernández for their valuable suggestions concerning the manuscript, and Jorge Miranda and his team from the Gastroendoscopy Service of the México Hospital for their collaboration.

This work was supported by the Research Vicepresident of the University of Costa Rica.

Recebido para publicação em 3/6/1991

Aceito para publicação em 11/2/1992

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Publication Dates

  • Publication in this collection
    14 Sept 2006
  • Date of issue
    Apr 1992

History

  • Accepted
    11 Feb 1992
  • Received
    03 June 1991
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