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vol.35 issue1Transmission of Schistosoma mansoni under experimental conditions using the bovine - Biomphalaria glabrata - bovine modelEvaluation in mice, of the antivenom efficacy injected at the same place of the intramuscular inoculation of the Crotalus durissus terrificus venom author indexsubject indexarticles search
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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.35 no.1 São Paulo Jan./Feb. 1993

http://dx.doi.org/10.1590/S0036-46651993000100003 

ORIGINAL ARTICLES

 

Host cell adhesion to Schistosoma mansoni larvae in the peritoneal cavity of naive mice. Histological and scanning electron microscopic studies

 

Adesão celular às larvas de Schistosoma mansoni na cavidade peritoneal de camundongos normais. Estudos histológicos e microscopia eletrônica de varredura

 

 

Alan Lane de MeloI; Conceição Ribeiro da Silva MachadoII; Leógenes Horácio PereiraI

IDepartamento de Parasitologia do Instituto de Ciências Biológicas da UFMG
IIDepartamento de Morfologia do Instituto de Ciências Biológicas da UFMG

Address for correspondence

 

 


SUMMARY

Cercariae of Schistosoma mansoni inoculated into the peritoneal cavity of naive mice induced host cell adhesion to their surface, but after 90 minutes the number of adherent cells sharply decreased. The cell detachment is progressive and simultaneous to the cercaria-schistosomule transformation. The histological study showed mainly neutrophils in close contact with the larvae. Mononuclear cells and some eosinophils were occasionally seen surrounding the adherent neutrophils. The scanning electron microscopy showed cells displaying twisted microvilli and several microplicae contacting or spreading over the larval surface, and larvae completely surrounded by clusters of cells.
These results suggest that the neutrophils recognize molecules on the cercarial surface which induce their spreading.

Key words: Schistosoma mansoni; Cell adhesion; Neutrophils; Cercaria


RESUMO

A inoculação de cercárias de Schistosoma mansoni na cavidade peritoneal de camundongos normais induz uma aderência de células do hospedeiro a essas larvas. Essa adesão decresce rapidamente quando a larva infectante transforma-se em esquistossômulo. O destacamento das células é progressivo e simultâneo à transformação. Os métodos histológicos e a microscopia eletrônica de varredura mostraram que o neutrófilo é a célula predominante em estreito contacto com a larva. Células mononucleadas e eosinófilos foram observados rodeando o parasito, usualmente sem estar em contacto direto com a larva. Os resultados indicam que neutrófilos podem reconhecer, na superfície larvária, moléculas que induzem sua adesão e espalhamento.


 

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Texto completo disponível apenas em PDF.

 

 

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Address for correspondence:
Prof. Dr. Alan Lane de Melo
Departamento de Parasitologia do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais
Cx. P.2486
CEP 30161-970 Belo Horizonte - MG, Brasil

Recebido para publicação em 30/04/1992
Aceito para publicação em 02/12/1992
Supported by The Parasitic Diseases Programme - WHO, Switzerland, CNPq, FINEP, and FAPEMIG, Brazil.

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