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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.35 no.2 São Paulo Mar./Apr. 1993

http://dx.doi.org/10.1590/S0036-46651993000200009 

DIAGNOSTIC TRIAL

 

Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies

 

Microteste de inibição de fluorescência simplificado para titulação de anticorpos antirábicos

 

 

S.R. FavorettoI; M.L. CarrieriI; M.S. TinoII; C.R. ZanettiI; O.A.C. PereiraI

IInstituto Pasteur - São Paulo. São Paulo, SP. Brasil
IIEscola Paulista de Medicina - Disciplina de Imunologia. São Paulo, SP. Brasil

Address for correspondence

 

 


SUMMARY

A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT).
The simplified test showed reproductibility similar to that of the FIMT with advantages as easier executation and quicker reading.
A simple pre-treatment of Brazilian microplates produced for immune enzymatic assays (PROSIL) gave equivalent results and substantial coast reduction, in relation to imported plates (DIFCO).
The simplified test can be easily implemented in less sophisticated laboratories, as alternative to the mouse serum neutralization test, still the most largely employed in Brazil, or even to others as RFFIT and FIMT.

Key words: Simplified Fluorescence Inhibition Microtest; rabies antibodies


RESUMO

No presente trabalho foi padronizada uma microtécnica simplificada para a avaliação de anticorpos neutralizantes anti-rábicos com base no RFFIT e no FIMT.
Este teste mostrou reprodutibilidade comparável a dos testes originais, sendo de execução mais simples e de leitura mais rápida.
Uma microplaca de fabricação nacional para testes imuno-enzimáticos (PROSIL Ind. e Com.) pode ser utilizada com resultados equivalentes aos de microplacas Difco ou similar, com substancial redução de custos, mediante tratamento simples e rápido.
O teste estudado pode ser implantado facilmente em laboratórios menos sofisticados substituindo com grandes vantagens a prova de soro-neutralização em camundongos ainda a mais empregada rotineiramente no Brasil.


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 


ACKNOWLEDGEMENTS

The authors gratefully acknowledge Mrs. Dolores Ayako Yoda, Mrs. Maria Aparecida da Silva and Mr. José Luiz de Carvalho Jr. for the technical assistence. This work was supported by The Health Secretary of the State of São Paulo, CNPq and FINEP.

 

REFERENCES

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2. GOUGH, P.M. & DIERKS, R.E. - Passive hemagglutina-üon test for antibodies against rabies virus. Bull. Wld. Hlth. Org., 45: 741-745, 1971.         [ Links ]

3. JOHNSON, H.N. & LEACH, C.N. - Comparative susceptibility of different strains of mice to rabies virus. Amer. J. Hyg., 32: 38-45, 1940.         [ Links ]

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11. ZANETTI, C.R.; TINO, M.S.; CHAMELET, E.L.B.; ISHIZUKA, M.M. & PEREIRA, O.A.C. - Simplification of immune adherence hemagglulination test for detection of rabies antibodies in human serum. Rev. Inst. Med. trop. S. Paulo, 31: 341-345, 1989.         [ Links ]

 

 

Address for correspondence:
Silvana R. Favoretto
Instituto Pasteur-São Paulo
Avenida Paulista, 393
CEP 01311-000 - São Paulo - SP - Brasil

Recebido para publicação em 7/8/1992
Aceito para publicação em 3/3/1993.

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