Desenvolvimento do Schistosoma mansoni na cavidade peritoneal de camundongos da linhagem AKR/J

Bicalho, Rosilene S.; Melo, Alan L. de; Pereira, Leógenes H.

doi:10.1590/S0036-46651993000500005

Resumos

Cercárias de Schistosoma mansoni, inoculadas na cavidade peritoneal de camundongos da linhagem AKR/J, conseguiram sobreviver in situ e chegar à maturidade sexual. Ao contrário da linhagem convencional (SWISS), onde as fêmeas que se desenvolveram no peritônio não produziram ovos, 7,7% das fêmeas retiradas da cavidade peritoneal de camundongos AKR/J apresentavam ovo normal no útero. Os parasitos recuperados da cavidade peritoneal de ambas as linhagens não apresentaram pigmento hemoglobínico, indicando que os mesmos sobrevivem na cavidade peritoneal de camundongos sem a necessidade de ingestão de hemácias. O desenvolvimento do parasito na cavidade peritoneal de camundongos AKR/J, com produção de ovos normais, reforça os dados, já existentes na literatura, que mostram que o ciclo evolutivo do parasito pode ser completado sem a necessidade da fase pulmonar.

Schistosoma mansoni; Cercária; AKR/J; Cavidade peritoneal

Cercariae of Schistossoma mansoni after intraperitoneal inoculation, developed and reached the sexual maturation in that site with egg production. In the AKR/J strain of mice, 7.7% of the peritoneal recovered females showed normal eggs in the uterus. No evidence of hemoglobinic pigment in their digestive tract was observed in the peritoneal recovered parasites from both strains (AKR/J and SWISS). This fact suggests that the parasites can develop without red blood cells ingestion. On the other hand, the development of the parasite with egg prodution in the peritoneal cavity of the AKR/J mouse reinforces the data that the lung phase is not necessary for the development of the parasite.

ARTIGOS ORIGINAIS

Desenvolvimento do Schistosoma mansoni na cavidade peritoneal de camundongos da linhagem AKR/J

Development of Schistosoma mansoni in the peritoneal cavity of the AKR/J strain of mice

Rosilene S. Bicalho; Alan L. de Melo; Leógenes H. Pereira

Grupo Interdepartamental de Estudos sobre Esquistossomose/Departamento de Parasitologia, Instituto de Ciências Biológicas da UFMG, 31270-901 Belo Horizonte, Brasil

RESUMO

Cercárias de Schistosoma mansoni, inoculadas na cavidade peritoneal de camundongos da linhagem AKR/J, conseguiram sobreviver in situ e chegar à maturidade sexual. Ao contrário da linhagem convencional (SWISS), onde as fêmeas que se desenvolveram no peritônio não produziram ovos, 7,7% das fêmeas retiradas da cavidade peritoneal de camundongos AKR/J apresentavam ovo normal no útero.

Os parasitos recuperados da cavidade peritoneal de ambas as linhagens não apresentaram pigmento hemoglobínico, indicando que os mesmos sobrevivem na cavidade peritoneal de camundongos sem a necessidade de ingestão de hemácias.

O desenvolvimento do parasito na cavidade peritoneal de camundongos AKR/J, com produção de ovos normais, reforça os dados, já existentes na literatura, que mostram que o ciclo evolutivo do parasito pode ser completado sem a necessidade da fase pulmonar.

Unitermos:Schistosoma mansoni; Cercária; AKR/J; Cavidade peritoneal.

SUMMARY

Cercariae of Schistossoma mansoni after intraperitoneal inoculation, developed and reached the sexual maturation in that site with egg production. In the AKR/J strain of mice, 7.7% of the peritoneal recovered females showed normal eggs in the uterus. No evidence of hemoglobinic pigment in their digestive tract was observed in the peritoneal recovered parasites from both strains (AKR/J and SWISS). This fact suggests that the parasites can develop without red blood cells ingestion. On the other hand, the development of the parasite with egg prodution in the peritoneal cavity of the AKR/J mouse reinforces the data that the lung phase is not necessary for the development of the parasite.

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Recebido para publicação em 15/01/1993.

Aceito para publicação em 28/06/1993.

Trabalho financiado com auxílio da FAPEMIG; CNPq e FINEP.

1. BEN-EFRAIM, S. & CINADER, B. - The role of complement in the passive cutaneous reaction of mice. J. exp. Med., 120: 925-942, 1964.
2. BENTLEY, A.G.; CARLISLE, A.S. & PHILLIPS, S.M. - Ultrastructural analysis of the cellular response to Schistosoma mansoni. Amer. J. trop. Med. Hyg., 30: 815-824, 1981.
3. CRAM, E.B. & BOZICEVICH, J. - Experimental Schistosoma mansoni infection by intraperitoneal injection. Trop. Med. News, 1: 16-17, 1944.
4. DAWKINS, H.J.S.; GROVE, D.I.; DUNSMORE, J.D. & MITCHELL, G.F. - Strongyloides ratti: susceptibility to infection and resistance to reinfection in inbred strains of mice as assessed by excretion of larvae. Int. J. Parasit., 10: 125-129, 1980.
5. EVANGELISTA, M.G.B.F. & BRESSAN, M.C.R.V. - Estudo da susceptibilidade de camundongos de diferentes linhagens isogęnicas por Hymenolepis nana var. fraterna (Stilles, 1909). In: CONGRESSO BRASILEIRO DE PARASITOLOGIA, 11, Rio de Janeiro, 1989. RESUMOS. p. 100.
6. FAN, P.C. & CHIANG, S.J. - Recovery, distribution and development of Schistosoma mansoni in mice following unisexual intraperitoneal infection with suggestion of a possible route of migration of schistosomes of man in mammalian hosts. Chin. J. Microbiol., 4: 182-189, 1971.
7. FAUST, E.C. & MELENEY, H.E. - Studies on schistosomiasis japonica. Amer. J. Hyg., 3: 1-339,1924.
8. HOLANDA, J.C. - Schistosoma mansoni: viabilidade no camundongo albino de esquistossômulos e formas intermediárias obtidas in vitro, esquistossômulos recuperados in vivo e cercárias por via venosa. Belo Horizonte, 1973. (Dissertaçăo de Mestrado - Instituto de Cięncias Biológicas da Universidade Federal de Minas Gerais).
9. INCANI, R.N. & McLAREN, D.J. - Neutrophil-mediated cytotoxicity to schistosomula of Schistosoma mansoni in vitro: studies on the kinetics of complement and/or antibody-dependent adherence and killing. Paras. Immunol., 3: 107-126, 1981.
10. LANA, M.; TAFURI, W.L.; CHIARI, H.; CALIARI, M.V.; FURTADO, D.F. & LEMOS, E.M. - Biological and histopathological behaviour of T. cruzi in different strains of mice. Mem. Inst. Oswaldo Cruz, 84 (suppl II): 26, 1989.
11. MACHADO, A.J.; GAZZINELLI, G.; PELLEGRINO, J. & DIAS DA SILVA, W. - Schistosoma mansoni: the role of the complement of C3 - activating system in the cercaricidal action of normal serum. Exp. Parasit., 38: 20, 1975.
12. MELO, A.L.; MACHADO, C.R.S. & PEREIRA, L.H. - Host cell adhesion to Schistosoma mansoni larvae in the peritoneal cavity of naive mice. Histological and scanning electron-microscopic studies. Rev. Inst. Med. trop. S. Paulo, 35: 17-22, 1993.
13. MOORE, D.V. & MELENEY, H.E. - Development of Schistosoma mansoni in the peritoneal cavity of mice. J. Parasit., 41: 235-245, 1955.
14. PEREIRA, L.H.; COELHO, P.M.Z.; FONSECA, J.J.A.; BREDET, A. & PELLEGRINO, J. - Migration of Schistosoma mansoni larvae in the albino mouse. Rev. Inst. Med. trop. S. Paulo, 14: 306-309, 1972.
15. PEREIRA, L.H.; PELLEGRINO, J.; VALADARES, T.E.; MELLO, R.T & COELHO, P.M.Z. - A new approach for screening prophylactic agents in schistosomiasis. Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.
16. RIVERA-ORTIZ, C.I. & NUSSENZWEIG, R. - Trichinella spiralis: anaphylactic antibody formation and susceptibility in strains of inbred mice. Exp. Parasit., 39: 7-17, 1976.
17. ROCHA, M.O. & COELHO, P.M.Z. - The importance of skin and pulmonary phases to the development of Schistosoma mansoni in albino mice. Rev. Inst. Med. trop. S. Paulo, 22: 157-163, 1980.
18. SAVAGE, A.M. & COLLEY, D.G. - The eosinophil in the inflammatory response to cercarial challenge of sensitized and chronically infected CBA/J mice. Amer. J. trop. Med. Hyg., 29: 1268-1278, 1980.
19. SHAW, M.K. - Schistosoma mansoni: vitelline gland development in females from single sex infections. J. Helminth., 61: 253-259, 1987.
20. SIMMONS, M.L. & BRICK, J.O. - The Laboratory mouse. Englewood Cliffs, N.J., Prentice-Hall, 1970.
21. STIREWALT, M.A.; KUNTZ, R.E. & EVANS, A.S. - The relative susceptibilities of the commonly used laboratory mammals to infection by Schistosoma mansoni. Amer. J. trop. Med. Hyg., 31: 57-82, 1951.
22. VAZ, N.M.; QUAGLIATA-PHILLIPS, J.M.; LEVINE, B.B. & VAZ E.M. - H-2 linked genetic control of immune responsiveness to ovalbiunin and ovomucoid. J. exp. Med., 134: 1333-1348, 1971.
23. WAWRIK, S.O.; NOVOTNY, J.R.; WICKS, I.P.; WILKINSON, D.; MAILER, D.; SALVARIS,E.; WELCH, K.; FECONDO, J. & BOYD, A.W. - The role of the LFA-1/ICAM-1 interaction in human leukocyte homing and adhesion. Immun. Rev., 108: 135-161, 1989.
24. YOLLES, T.K.; MOORE, D.V. & MELENEY, H.E. - Post-cercarial development of Schistosoma mansoni in the rabbit and hamster after intraperitoneal and percutaneous infection. J. Parasit., 35: 276-294, 1949.

Datas de Publicação

Publicação nesta coleção
04 Jul 2006
Data do Fascículo
Out 1993

Histórico

Aceito
28 Jun 1993
Recebido
15 Jan 1993

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BEN-EFRAIM, S. & CINADER, B. - The role of complement in the passive cutaneous reaction of mice. J. exp. Med., 120: 925-942, 1964.

[2] 2. BENTLEY, A.G.; CARLISLE, A.S. & PHILLIPS, S.M. - Ultrastructural analysis of the cellular response to Schistosoma mansoni. Amer. J. trop. Med. Hyg., 30: 815-824, 1981.

[3] 3. CRAM, E.B. & BOZICEVICH, J. - Experimental Schistosoma mansoni infection by intraperitoneal injection. Trop. Med. News, 1: 16-17, 1944.

[4] 4. DAWKINS, H.J.S.; GROVE, D.I.; DUNSMORE, J.D. & MITCHELL, G.F. - Strongyloides ratti: susceptibility to infection and resistance to reinfection in inbred strains of mice as assessed by excretion of larvae. Int. J. Parasit., 10: 125-129, 1980.

[5] 5. EVANGELISTA, M.G.B.F. & BRESSAN, M.C.R.V. - Estudo da susceptibilidade de camundongos de diferentes linhagens isogęnicas por Hymenolepis nana var. fraterna (Stilles, 1909). In: CONGRESSO BRASILEIRO DE PARASITOLOGIA, 11, Rio de Janeiro, 1989. RESUMOS. p. 100.

[6] 6. FAN, P.C. & CHIANG, S.J. - Recovery, distribution and development of Schistosoma mansoni in mice following unisexual intraperitoneal infection with suggestion of a possible route of migration of schistosomes of man in mammalian hosts. Chin. J. Microbiol., 4: 182-189, 1971.

[7] 7. FAUST, E.C. & MELENEY, H.E. - Studies on schistosomiasis japonica. Amer. J. Hyg., 3: 1-339,1924.

[8] 8. HOLANDA, J.C. - Schistosoma mansoni: viabilidade no camundongo albino de esquistossômulos e formas intermediárias obtidas in vitro, esquistossômulos recuperados in vivo e cercárias por via venosa. Belo Horizonte, 1973. (Dissertaçăo de Mestrado - Instituto de Cięncias Biológicas da Universidade Federal de Minas Gerais).

[9] 9. INCANI, R.N. & McLAREN, D.J. - Neutrophil-mediated cytotoxicity to schistosomula of Schistosoma mansoni in vitro: studies on the kinetics of complement and/or antibody-dependent adherence and killing. Paras. Immunol., 3: 107-126, 1981.

[10] 10. LANA, M.; TAFURI, W.L.; CHIARI, H.; CALIARI, M.V.; FURTADO, D.F. & LEMOS, E.M. - Biological and histopathological behaviour of T. cruzi in different strains of mice. Mem. Inst. Oswaldo Cruz, 84 (suppl II): 26, 1989.

[11] 11. MACHADO, A.J.; GAZZINELLI, G.; PELLEGRINO, J. & DIAS DA SILVA, W. - Schistosoma mansoni: the role of the complement of C3 - activating system in the cercaricidal action of normal serum. Exp. Parasit., 38: 20, 1975.

[12] 12. MELO, A.L.; MACHADO, C.R.S. & PEREIRA, L.H. - Host cell adhesion to Schistosoma mansoni larvae in the peritoneal cavity of naive mice. Histological and scanning electron-microscopic studies. Rev. Inst. Med. trop. S. Paulo, 35: 17-22, 1993.

[13] 13. MOORE, D.V. & MELENEY, H.E. - Development of Schistosoma mansoni in the peritoneal cavity of mice. J. Parasit., 41: 235-245, 1955.

[14] 14. PEREIRA, L.H.; COELHO, P.M.Z.; FONSECA, J.J.A.; BREDET, A. & PELLEGRINO, J. - Migration of Schistosoma mansoni larvae in the albino mouse. Rev. Inst. Med. trop. S. Paulo, 14: 306-309, 1972.

[15] 15. PEREIRA, L.H.; PELLEGRINO, J.; VALADARES, T.E.; MELLO, R.T & COELHO, P.M.Z. - A new approach for screening prophylactic agents in schistosomiasis. Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.

[16] 16. RIVERA-ORTIZ, C.I. & NUSSENZWEIG, R. - Trichinella spiralis: anaphylactic antibody formation and susceptibility in strains of inbred mice. Exp. Parasit., 39: 7-17, 1976.

[17] 17. ROCHA, M.O. & COELHO, P.M.Z. - The importance of skin and pulmonary phases to the development of Schistosoma mansoni in albino mice. Rev. Inst. Med. trop. S. Paulo, 22: 157-163, 1980.

[18] 18. SAVAGE, A.M. & COLLEY, D.G. - The eosinophil in the inflammatory response to cercarial challenge of sensitized and chronically infected CBA/J mice. Amer. J. trop. Med. Hyg., 29: 1268-1278, 1980.

[19] 19. SHAW, M.K. - Schistosoma mansoni: vitelline gland development in females from single sex infections. J. Helminth., 61: 253-259, 1987.

[20] 20. SIMMONS, M.L. & BRICK, J.O. - The Laboratory mouse. Englewood Cliffs, N.J., Prentice-Hall, 1970.

[21] 21. STIREWALT, M.A.; KUNTZ, R.E. & EVANS, A.S. - The relative susceptibilities of the commonly used laboratory mammals to infection by Schistosoma mansoni. Amer. J. trop. Med. Hyg., 31: 57-82, 1951.

[22] 22. VAZ, N.M.; QUAGLIATA-PHILLIPS, J.M.; LEVINE, B.B. & VAZ E.M. - H-2 linked genetic control of immune responsiveness to ovalbiunin and ovomucoid. J. exp. Med., 134: 1333-1348, 1971.

[23] 23. WAWRIK, S.O.; NOVOTNY, J.R.; WICKS, I.P.; WILKINSON, D.; MAILER, D.; SALVARIS,E.; WELCH, K.; FECONDO, J. & BOYD, A.W. - The role of the LFA-1/ICAM-1 interaction in human leukocyte homing and adhesion. Immun. Rev., 108: 135-161, 1989.

[24] 24. YOLLES, T.K.; MOORE, D.V. & MELENEY, H.E. - Post-cercarial development of Schistosoma mansoni in the rabbit and hamster after intraperitoneal and percutaneous infection. J. Parasit., 35: 276-294, 1949.

Brasil

Brasil

Desenvolvimento do Schistosoma mansoni na cavidade peritoneal de camundongos da linhagem AKR/J

Development of Schistosoma mansoni in the peritoneal cavity of the AKR/J strain of mice

Resumos

Datas de Publicação

Histórico