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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.35 no.6 São Paulo Nov./Dec. 1993

http://dx.doi.org/10.1590/S0036-46651993000600005 

PARASITOLOGY

 

An attemp at reversibility and increase of the virulence of axenic strains of Entamoeba histolytica

 

Tentativa de reversibilidade e aumento de virulência de cepas axônicas de Entamoeba histolytica

 

 

Maria Aparecida GomesI; Adriana Oliveira CostaI; Washington Luiz TafuriII; Edward Félix SilvaI

IDepartment of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
IIDepartment of Biological Sciences, Institute of Biological and Exact Sciences, Federal University of Ouro Preto, Ouro Preto, MG, Brazil

Address for correspondence

 

 


SUMMARY

In this study we have tried to verify whether the interaction "in vitro" with bacteria or small pieces of normal hamster liver would modify the pathogenic behavior of axenic strains of E. histolytica: avirulent ones (ICB-32 and ICB-RPS), of attenuated virulence (ICB-CSP and HM1) and of mean virulence (ICB-462). Every attempt to render virulent, recover or increase the virulence of axenic strains of E. histolytica has failed.

Keywords: Entamoeba histolytica; Axenic cultures; Ameba-bacterium interaction; Virulence.


RESUMO

Neste trabalho procuramos verificar se a interação "in vitro" com bactérias e fragmentos de fígado de hamster normal, modificaria o comportamento patogênico de cepas axênicas de E. histolytica avirulentas (ICB-32 e ICB-RPS); virulentas, porém atenuadas (ICB-CSP e HM1) e de média virulência (ICB-462). Todas as tentativas de tornar virulentas, restabelecer ou aumentar a virulência das cepas axênicas de E. histolytica utilizadas fracassaram.


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 

 

ACKNOWLEDGEMENTS

We are indebted with the Development Center of Nuclear Technology by irradiation service and to Marcia A. Silva for able technical assistance. We are grateful to Dr. Paul Williams for correcting the manuscript. This work was supported by CNPq, FINEP and FAPEMIG.

 

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Address for correspondence:
Dr. Edward Félix Silva
Departamentode Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais
Av. Antônio Carlos, 6627
CEP 31270-010, Belo Horizonte, Minas Gerais, Brasil

Recebido para publicação em 15/02/1993
Aceito para publicação em 23/08/1993.

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