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Revista do Instituto de Medicina Tropical de São Paulo

versão impressa ISSN 0036-4665

Rev. Inst. Med. trop. S. Paulo v.35 n.6 São Paulo nov./dez. 1993

http://dx.doi.org/10.1590/S0036-46651993000600007 

VIROLOGY

 

Detection of hepatitis B virus DNA by the polymerase chain reaction in anti-HBE positive chronic hepatitis B patients

 

Detecção do DNA do vírus da Hepatite B pela reação em cadeia da polimerase em pacientes com Hepatite Crônica B soropositivos para o anticorpo Anti-HBe

 

 

J.R.R. PinhoI,II,III; C.A. SantosII; C.L.M. GonzalezIV; L. BassitI; C.C. BarretoI; A. Saez-AlquezarI; A.V.C. FrançaIV; F.J. CarrilhoIV; L.E.P. FonsecaIV; D.A.F. ChamoneI; L. C. da SilvaIII

IFundação Pró-Sangue Hemocentro de São Paulo. São Paulo, SP, Brasil
IIVirology Branch - Instituto Adolfo Lutz. São Paulo, SP, Brasil
IIIVirology and Hepatology Laboratories - Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brasil
IVGastroenterology Branch - Faculdade de Medicina da Universidade de São Paulo. São Paulo, SP, Brasil

Address for correspondence

 

 


SUMMARY

Detection of HBV-DNA by PCR was compared with other serological markers (HBsAg, HBeAg and anti-HBe) in a series of49 Chronic Hepatitis B patients, including 12 with a spontaneous clearance of HBsAg. None of these HBsAg negative cases were PCR positive, but 33/37 (89.2%) HBsAg positive cases were PCR positive (p < 0.0001). Among HBsAg positive samples, nine cases were HBeAg positive and anti-HBe negative, all of them PCR positive. Other 3 patients were HBeAg and anti-HBe positive and these cases were also found PCR positive. A third group included 21 patients anti-HBe positive and HBeAg negative: 19 of them were PCR positive and 2 were PCR negative. The last 4 cases were HBeAg and anti-HBe negative, two of them were PCR positive.
The detection of anti-HBe viremic cases in the present series suggest that preC variants could occur in our country. In conclusion, the integrated phase o f chronic hepatitis B seems to be less frequent than it was assumed, when only HBeAg or dot blot hybridization techniques were used. The new term "low replication phase" might favorably replace the former "integrated phase".

Keywords: HBV; PCR; replication Markers; chronic Hepatitis


RESUMO

A detecção do DNA do Vírus da Hepatite B pela Reação em Cadeia da Polimerase (PCR) foi comparada com os outros marcadores sorológicos virais (AgHBs, AgHBe e anti-HBe) numa série de 49 pacientes com hepatite crônica B, incluindo 12 que apresentaram clareamento espontâneo do AgHBs. Nenhum caso AgHBs negativo foi PCR positivo, mas 33/37 (89,2%) dos casos AgHBs positivos foram PCR positivos (p < 0,0001). Entre as amostras AgHBs positivas, 9 foram AgHBc positivas e anti-HBe negativas, todas elas PCR positivas. Outros 3 pacientes foram AgHBe e anti-HBe positivos, todos também PCR positivos. Um terceiro grupo incluía 21 pacientes anti-HBe positivos e anti-HBe negativos: 19 foram PCR positivos e 2 PCR negativos. Os últimos 4 casos foram AgHBe e anti-HBe negativos, sendo 2 destes PCR positivos. A detecção de casos virêmicos anti-HBe positivos sugere que as variantes preC do VHB podem também estar presentes em nosso país. Em conclusão, a "fase de integração viral" da hepatite crônica B parece ser muito menos frequente do que se imaginava previamente, quando apenas se dispunha das técnicas de detecção de AgHBe e DNA por hibridização. O novo termo "fase de baixa replicação baixa" parece ser melhor que o anterior "fase de integração viral" .


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 

 

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Address for correspondence:
João Renato Rebello Pinho
Instituto de Medicina Tropical de São Paulo
Av. Dr. Enéas de Carvalho Aguiar, 470
05403-000 São Paulo, SP, Brasil

Recebido para publicação em 22/06/1993
Aceito para publicação em 27/08/1993.