Decreased erytrocyte osmotic fragility during canine leptospirosis

Santoro, Marcelo L.; Kogika, Marcia M.; Hagiwara, Mitika K.; Mirandola, Regina M. S.; Castelar, Izaura L. C. G.

doi:10.1590/S0036-46651994000100001

Abstracts

Erythrocyte osmotic fragility (EOF) was carried out in nineteen dogs naturally infected by Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. A decreased EOF was observed, suggesting a modification of erythrocyte components secondary to disturbances that occur during canine leptospirosis, such as renal damage and hepatic disease.

Dog; Osmotic resistance; Red blood cell

A fragilidade osmótica eritrocitária foi estudada em dezenove cães infectados naturalmente pela Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. Observou-se uma redução da fragilidade osmótica eritrocitária, sem a presença de anemia, possivelmente relacionada aos distúrbios hepato-renais que ocorrem nesta patologia.

MICROBIOLOGY

Decreased erytrocyte osmotic fragility during canine leptospirosis

Diminuição da fragilidade osmótica eritrocitária na leptospirose canina

Marcelo L. Santoro^I; Marcia M. Kogika^II; Mitika K. Hagiwara^II; Regina M. S. Mirandola^II; Izaura L. C. G. Castelar^II

^ILaboratory of Pathophysiology-Institute Butantan. São Paulo, SP, Brazil

^IIDepartment of Medical Clinics, College of Veterinary Medicine, University of São Paulo, SP, Brazil

^{Address for correspondence} Address for correspondence: Marcelo L. Santoro Instituto Butantan, Lab. Fisiopatologia Av. Vital Brasil, 1500 05503-900 São Paulo, SP, Brazil

SUMMARY

Erythrocyte osmotic fragility (EOF) was carried out in nineteen dogs naturally infected by Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. A decreased EOF was observed, suggesting a modification of erythrocyte components secondary to disturbances that occur during canine leptospirosis, such as renal damage and hepatic disease.

Keywords: Dog; Osmotic resistance; Red blood cell.

RESUMO

A fragilidade osmótica eritrocitária foi estudada em dezenove cães infectados naturalmente pela Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. Observou-se uma redução da fragilidade osmótica eritrocitária, sem a presença de anemia, possivelmente relacionada aos distúrbios hepato-renais que ocorrem nesta patologia.

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Recebido para publicação em 09/06/1993.

Aceito para publicação em 01/10/1993.

1. ALEXANDER, A.D.; SMITH, O.H.; HIATT, C.W. & GLEISER, C.A. - Presence of hemolysin in cultures of pathogenic leptospires. Proc. Soc. exp. Biol. (N. Y.), 91: 205-211, 1956.
2. BERNHEIMER, A.W. & BEY, R.F. - Copurification of Leptospira interrogam serovar pomona hemolysin and sphingomyelinase C. Infect. Immun., 54: 262-264, 1986.
3. BIRGEL, E.H. & BENESI, F.J. - Patologia clínica veterinária. Săo Paulo, SPMV, 1982. p. 2-34.
4. BOGIN, E.; MASSRY, S.G.; LEVI, J. et al. - Effect of parathyroid hormone on osmotic fragility of human erythrocytes. J. clin. Invest., 69: 1017-1025, 1982.
5. COOPER, R.A. & JANDL, J.H. - Bile salts cholesterol in the pathogenesis of target cells in obstructive jaundice. J. clin. Invest., 47: 809-822, 1968.
6. DOCCI, D.; TURCI, F. & BALDRATI, L. - Lack of relation between secondary hyperparathyroidism and red blood cell osmotic fragility in chronic renal failure. Nephron, 41: 241-245, 1985.
7. FINCO, D.R. & LOW, D.G. - Water, electrolyte, and acid-base alterations in experimental canine leptospirosis. Amer. J. vet. Res., 29: 1799-1807, 1968.
8. GAFTER, U.; MALACHI, T.; BARAK, H. & LEVI, J. - Red blood cell calcium level in chronic renal failure: effect of continuous ambulatory peritoneal dialysis. J. Lab. clin. Med., 116: 386-392, 1990.
9. GIBER, U.; HARVEY, J.W.; YAMAGUCHI, R.A. et al. - Inherited phosphofructokinase deficiency in dogs with hyperventilation-induced hemolysis: increased in vitro and in vivo alkaline fragility of erythrocytes. Blood, 65: 345-351, 1985.
10. JAIN, N.C. - Schalm's veterinary hematology. 4. ed. Philadelphia, Lea & Febiger, 1986.
11. KASŔROV, L.B. - Degradation of the erythrocyte of different animal species by leptospirae. J. med. Microbiol., 3: 29-37, 1970.
12. KOGIKA, M.M.; HAGIWARA, M.K.; YASUDA, P.H. & MIRANDOLA, R.M.S. - Alteraçőes hematológicas na leptospirose canina. Rev. Fac. Med. vet. Univ. S. Paulo, 24: 41-46, 1989.
13. OYABU, H.; TSUMOTO, S. & KAGEYAMA, T. - Clinical investigation on the osmotic fragility of erythrocytes in the cirrhosis of liver. Bull. Osaka med. Sch., 28: 33-45, 1982.
14. PARPART, A.K.; LORENZ, P.B.; PARPART, E.R.; GREGG, J.R. & CHASE, A.M. - The osmotic resistance (fragility) of human red cells. J. clin. Invest., 26: 636-640, 1947.
15. SAGAWA, S. & SHIRAKI, K. - Role of lipids in stabilizing red cells in rats. J. nutr. Sci. Vitaminol., 24: 57-65, 1978.
16. SANTA ROSA, C.A. - Diagnóstico laboratorial das leptospiroses. Rev. Microbiol., 1: 97-109, 1970.
17. SASAKI, S. - Red cell lipids in patients with end-stage renal failure: relationship to osmotic fragility. Osaka Cy. med. J., 23: 85-98, 1977.
18. SUESS, J.; LIMENTANI, D.; DAMESHEK, W. & DOLLOFF, M.J. - A quantitative method for the determination and charting of the erythrocyte hypotonic fragility. Blood, 3: 1290-1303, 1948.
19. THOMPSON, J.C. - Morphological changes in red blood cells of calves caused by Leptospira interrogans serovar pomona. J. comp. Path., 96: 517-527, 1986.
20. TROWBRIDGE, A.A.; GREEN, J.B.; BONNETT, J.D. et al. - Hemolytic anemia associated with leptospirosis. Morphologic and lipid studies. Amer. J. clin. Path., 76: 493-498. 1981.
21. WADDELL, W.J. - Lysis of dogs erythrocytes in mildly alkaline isotonic media. Amer. J. Physiol., 186: 339-342, 1956.
22. WERRE, J.M.; HELLEMAN, P.W.; VERLOOP, M.C. & DE GIER, J. - Causes of macroplania of erythrocytes in diseases of the liver and biliary tract with special reference to leptocytosis. Brit. J. Haemat, 19: 223-235, 1970.

Address for correspondence:

Marcelo L. Santoro

Instituto Butantan, Lab. Fisiopatologia

Av. Vital Brasil, 1500

05503-900 São Paulo, SP, Brazil

Publication Dates

Publication in this collection
20 Sept 2006
Date of issue
Feb 1994

History

Accepted
01 Oct 1993
Received
09 June 1993

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALEXANDER, A.D.; SMITH, O.H.; HIATT, C.W. & GLEISER, C.A. - Presence of hemolysin in cultures of pathogenic leptospires. Proc. Soc. exp. Biol. (N. Y.), 91: 205-211, 1956.

[2] 2. BERNHEIMER, A.W. & BEY, R.F. - Copurification of Leptospira interrogam serovar pomona hemolysin and sphingomyelinase C. Infect. Immun., 54: 262-264, 1986.

[3] 3. BIRGEL, E.H. & BENESI, F.J. - Patologia clínica veterinária. Săo Paulo, SPMV, 1982. p. 2-34.

[4] 4. BOGIN, E.; MASSRY, S.G.; LEVI, J. et al. - Effect of parathyroid hormone on osmotic fragility of human erythrocytes. J. clin. Invest., 69: 1017-1025, 1982.

[5] 5. COOPER, R.A. & JANDL, J.H. - Bile salts cholesterol in the pathogenesis of target cells in obstructive jaundice. J. clin. Invest., 47: 809-822, 1968.

[6] 6. DOCCI, D.; TURCI, F. & BALDRATI, L. - Lack of relation between secondary hyperparathyroidism and red blood cell osmotic fragility in chronic renal failure. Nephron, 41: 241-245, 1985.

[7] 7. FINCO, D.R. & LOW, D.G. - Water, electrolyte, and acid-base alterations in experimental canine leptospirosis. Amer. J. vet. Res., 29: 1799-1807, 1968.

[8] 8. GAFTER, U.; MALACHI, T.; BARAK, H. & LEVI, J. - Red blood cell calcium level in chronic renal failure: effect of continuous ambulatory peritoneal dialysis. J. Lab. clin. Med., 116: 386-392, 1990.

[9] 9. GIBER, U.; HARVEY, J.W.; YAMAGUCHI, R.A. et al. - Inherited phosphofructokinase deficiency in dogs with hyperventilation-induced hemolysis: increased in vitro and in vivo alkaline fragility of erythrocytes. Blood, 65: 345-351, 1985.

[10] 10. JAIN, N.C. - Schalm's veterinary hematology. 4. ed. Philadelphia, Lea & Febiger, 1986.

[11] 11. KASŔROV, L.B. - Degradation of the erythrocyte of different animal species by leptospirae. J. med. Microbiol., 3: 29-37, 1970.

[12] 12. KOGIKA, M.M.; HAGIWARA, M.K.; YASUDA, P.H. & MIRANDOLA, R.M.S. - Alteraçőes hematológicas na leptospirose canina. Rev. Fac. Med. vet. Univ. S. Paulo, 24: 41-46, 1989.

[13] 13. OYABU, H.; TSUMOTO, S. & KAGEYAMA, T. - Clinical investigation on the osmotic fragility of erythrocytes in the cirrhosis of liver. Bull. Osaka med. Sch., 28: 33-45, 1982.

[14] 14. PARPART, A.K.; LORENZ, P.B.; PARPART, E.R.; GREGG, J.R. & CHASE, A.M. - The osmotic resistance (fragility) of human red cells. J. clin. Invest., 26: 636-640, 1947.

[15] 15. SAGAWA, S. & SHIRAKI, K. - Role of lipids in stabilizing red cells in rats. J. nutr. Sci. Vitaminol., 24: 57-65, 1978.

[16] 16. SANTA ROSA, C.A. - Diagnóstico laboratorial das leptospiroses. Rev. Microbiol., 1: 97-109, 1970.

[17] 17. SASAKI, S. - Red cell lipids in patients with end-stage renal failure: relationship to osmotic fragility. Osaka Cy. med. J., 23: 85-98, 1977.

[18] 18. SUESS, J.; LIMENTANI, D.; DAMESHEK, W. & DOLLOFF, M.J. - A quantitative method for the determination and charting of the erythrocyte hypotonic fragility. Blood, 3: 1290-1303, 1948.

[19] 19. THOMPSON, J.C. - Morphological changes in red blood cells of calves caused by Leptospira interrogans serovar pomona. J. comp. Path., 96: 517-527, 1986.

[20] 20. TROWBRIDGE, A.A.; GREEN, J.B.; BONNETT, J.D. et al. - Hemolytic anemia associated with leptospirosis. Morphologic and lipid studies. Amer. J. clin. Path., 76: 493-498. 1981.

[21] 21. WADDELL, W.J. - Lysis of dogs erythrocytes in mildly alkaline isotonic media. Amer. J. Physiol., 186: 339-342, 1956.

[22] 22. WERRE, J.M.; HELLEMAN, P.W.; VERLOOP, M.C. & DE GIER, J. - Causes of macroplania of erythrocytes in diseases of the liver and biliary tract with special reference to leptocytosis. Brit. J. Haemat, 19: 223-235, 1970.