Abstracts
Erythrocyte osmotic fragility (EOF) was carried out in nineteen dogs naturally infected by Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. A decreased EOF was observed, suggesting a modification of erythrocyte components secondary to disturbances that occur during canine leptospirosis, such as renal damage and hepatic disease.
Dog; Osmotic resistance; Red blood cell
A fragilidade osmótica eritrocitária foi estudada em dezenove cães infectados naturalmente pela Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. Observou-se uma redução da fragilidade osmótica eritrocitária, sem a presença de anemia, possivelmente relacionada aos distúrbios hepato-renais que ocorrem nesta patologia.
MICROBIOLOGY
Decreased erytrocyte osmotic fragility during canine leptospirosis
Diminuição da fragilidade osmótica eritrocitária na leptospirose canina
Marcelo L. SantoroI; Marcia M. KogikaII; Mitika K. HagiwaraII; Regina M. S. MirandolaII; Izaura L. C. G. CastelarII
ILaboratory of Pathophysiology-Institute Butantan. São Paulo, SP, Brazil
IIDepartment of Medical Clinics, College of Veterinary Medicine, University of São Paulo, SP, Brazil
Address for correspondence Address for correspondence: Marcelo L. Santoro Instituto Butantan, Lab. Fisiopatologia Av. Vital Brasil, 1500 05503-900 São Paulo, SP, Brazil
SUMMARY
Erythrocyte osmotic fragility (EOF) was carried out in nineteen dogs naturally infected by Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. A decreased EOF was observed, suggesting a modification of erythrocyte components secondary to disturbances that occur during canine leptospirosis, such as renal damage and hepatic disease.
Keywords: Dog; Osmotic resistance; Red blood cell.
RESUMO
A fragilidade osmótica eritrocitária foi estudada em dezenove cães infectados naturalmente pela Leptospira interrogans serovar icterohaemorrhagiae/copenhagi. Observou-se uma redução da fragilidade osmótica eritrocitária, sem a presença de anemia, possivelmente relacionada aos distúrbios hepato-renais que ocorrem nesta patologia.
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Recebido para publicação em 09/06/1993.
Aceito para publicação em 01/10/1993.
- 1. ALEXANDER, A.D.; SMITH, O.H.; HIATT, C.W. & GLEISER, C.A. - Presence of hemolysin in cultures of pathogenic leptospires. Proc. Soc. exp. Biol. (N. Y.), 91: 205-211, 1956.
- 2. BERNHEIMER, A.W. & BEY, R.F. - Copurification of Leptospira interrogam serovar pomona hemolysin and sphingomyelinase C. Infect. Immun., 54: 262-264, 1986.
- 3. BIRGEL, E.H. & BENESI, F.J. - Patologia clínica veterinária. Săo Paulo, SPMV, 1982. p. 2-34.
- 4. BOGIN, E.; MASSRY, S.G.; LEVI, J. et al. - Effect of parathyroid hormone on osmotic fragility of human erythrocytes. J. clin. Invest., 69: 1017-1025, 1982.
- 5. COOPER, R.A. & JANDL, J.H. - Bile salts cholesterol in the pathogenesis of target cells in obstructive jaundice. J. clin. Invest., 47: 809-822, 1968.
- 6. DOCCI, D.; TURCI, F. & BALDRATI, L. - Lack of relation between secondary hyperparathyroidism and red blood cell osmotic fragility in chronic renal failure. Nephron, 41: 241-245, 1985.
- 7. FINCO, D.R. & LOW, D.G. - Water, electrolyte, and acid-base alterations in experimental canine leptospirosis. Amer. J. vet. Res., 29: 1799-1807, 1968.
- 8. GAFTER, U.; MALACHI, T.; BARAK, H. & LEVI, J. - Red blood cell calcium level in chronic renal failure: effect of continuous ambulatory peritoneal dialysis. J. Lab. clin. Med., 116: 386-392, 1990.
- 9. GIBER, U.; HARVEY, J.W.; YAMAGUCHI, R.A. et al. - Inherited phosphofructokinase deficiency in dogs with hyperventilation-induced hemolysis: increased in vitro and in vivo alkaline fragility of erythrocytes. Blood, 65: 345-351, 1985.
- 10. JAIN, N.C. - Schalm's veterinary hematology. 4. ed. Philadelphia, Lea & Febiger, 1986.
- 11. KASŔROV, L.B. - Degradation of the erythrocyte of different animal species by leptospirae. J. med. Microbiol., 3: 29-37, 1970.
- 12. KOGIKA, M.M.; HAGIWARA, M.K.; YASUDA, P.H. & MIRANDOLA, R.M.S. - Alteraçőes hematológicas na leptospirose canina. Rev. Fac. Med. vet. Univ. S. Paulo, 24: 41-46, 1989.
- 13. OYABU, H.; TSUMOTO, S. & KAGEYAMA, T. - Clinical investigation on the osmotic fragility of erythrocytes in the cirrhosis of liver. Bull. Osaka med. Sch., 28: 33-45, 1982.
- 14. PARPART, A.K.; LORENZ, P.B.; PARPART, E.R.; GREGG, J.R. & CHASE, A.M. - The osmotic resistance (fragility) of human red cells. J. clin. Invest., 26: 636-640, 1947.
- 15. SAGAWA, S. & SHIRAKI, K. - Role of lipids in stabilizing red cells in rats. J. nutr. Sci. Vitaminol., 24: 57-65, 1978.
- 16. SANTA ROSA, C.A. - Diagnóstico laboratorial das leptospiroses. Rev. Microbiol., 1: 97-109, 1970.
- 17. SASAKI, S. - Red cell lipids in patients with end-stage renal failure: relationship to osmotic fragility. Osaka Cy. med. J., 23: 85-98, 1977.
- 18. SUESS, J.; LIMENTANI, D.; DAMESHEK, W. & DOLLOFF, M.J. - A quantitative method for the determination and charting of the erythrocyte hypotonic fragility. Blood, 3: 1290-1303, 1948.
- 19. THOMPSON, J.C. - Morphological changes in red blood cells of calves caused by Leptospira interrogans serovar pomona. J. comp. Path., 96: 517-527, 1986.
- 20. TROWBRIDGE, A.A.; GREEN, J.B.; BONNETT, J.D. et al. - Hemolytic anemia associated with leptospirosis. Morphologic and lipid studies. Amer. J. clin. Path., 76: 493-498. 1981.
- 21. WADDELL, W.J. - Lysis of dogs erythrocytes in mildly alkaline isotonic media. Amer. J. Physiol., 186: 339-342, 1956.
- 22. WERRE, J.M.; HELLEMAN, P.W.; VERLOOP, M.C. & DE GIER, J. - Causes of macroplania of erythrocytes in diseases of the liver and biliary tract with special reference to leptocytosis. Brit. J. Haemat, 19: 223-235, 1970.
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Publication Dates
-
Publication in this collection
20 Sept 2006 -
Date of issue
Feb 1994
History
-
Accepted
01 Oct 1993 -
Received
09 June 1993