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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.36 no.2 São Paulo Mar./Apr. 1994

http://dx.doi.org/10.1590/S0036-46651994000200011 

TECHNICAL TRIALS

 

Evaluation of different methods for Plasmodia detection, in well defined population groups in an endemic area of Brazil(1)

 

Avaliação de diferentes métodos para detecção de plasmódios em grupos populacionais bem definidos em uma área endêmica do Brasil

 

 

Sandra L. M. AvilaI; Mirlan C. LeandroI; Noemia B. CarvalhoII; Marilia S. OliveiraII; Viviana G. ArrukI; Maria Carmen A. SanchezI; Marcos BoulosI,II; Antonio Walter FerreiraI,II

IInstitute of Tropical Medicine of São Paulo - Medical School of the University of São Paulo
IIInfectious Diseases Department of the Medical School of the University of São Paulo

Address for correspondence

 

 


SUMMARY

In Brazil, more than 500,000 new cases of malaria were notified in 1992. Plasmodium falciparum and P.vivax are the responsible species for 99.3% of the cases. For adequate treatment, precoce diagnosis is necessary. In this work, we present the results of the traditional Plasmodia detection method, thick blood film (TBF), and the results of alternative methods: Immunofluorescence assay (IFA) with polyclonal antibody and Quantitative Buffy Coat method (QBC)® in a well defined population groups. The analysis were done in relation to the presence or absence of malaria clinical symptoms. Also different classes of immunoglobulins anti-P.falciparum were quantified for the global analysis of the results, mainly in the discrepant results. We concluded that alternative methods are more sensitive than TBF and that the association of epidemiological, clinical and laboratory findings is necessary to define the presence of malaria.

Keywords: Malaria; Plasmodia detection; diagnosis; IFA; QBC.


RESUMO

Mais de 500.000 casos novos de malária foram notificados no Brasil, em 1992. P.falciparum and P.vivax são as espécies responsáveis por 99,3% dos casos. O diagnóstico precoce é indispensável para início do tratamento adequado. Neste trabalho, apresentamos os resultados do método tradicional para detecção de plasmódios, gota espessa, e os resultados de métodos alternativos estudados: imunofluorescência indireta com anticorpo policlonal anti-P.falciparum e QBC-método, em grupos populacionais bem definidos. A análise dos resultados foi feita em relação à presença ou ausênsia de sintomas clínicos de malária. Também, diferentes classes de imunoglobulinas anti-P.falciparum foram quantificadas para auxiliar na análise global dos resultados, principalmente nos resultados discrepantes. Nós concluímos que os métodos alternativos são mais sensíveis do que a gota espessa e que a associação das informações epidemiológicas, clínicas e laboratoriais é necessária para definir a presença de malária.


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 

 

ACKNOWLEDGEMENTS

This work was in part supported by FAPESP (91/0338-0). We thank to Becton Dickinson for supplying the QBC tubes, Dr. Joseph Perrone for critical review of this manuscript, Mr. Nei Rodrigues de Carvalho Filho for technical assistance and Dr. Fernando Costa Andrade Moraes and Fundação Nacional de Saúde - Regional Mato Grsso.

 

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Address for correspondence:
Sandra do Lago Moraes de Ávila
Instituto de Medicina Tropical de São Paulo. Faculdade de Medicina da Universidade de São Paulo
Av. Dr. Enéas de Carvalho Aguiar, 470
05403-000 São Paulo, SP, Brasil

Recebido para publicação em 29/07/1993.
Aceito para publicação em 19/01/1994.

 

 

(1) The data presented in this work are part of a Ph thesis.

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