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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.36 no.4 São Paulo July/Aug. 1994

https://doi.org/10.1590/S0036-46651994000400005 

SEROLOGY

 

The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana

 

Importância diagnóstica da reação cruzada espécie-específica de componentes da Taenia solium, Echinococcus granulosus e Hymenolepis nana

 

 

Teresa MontenegroI; Robert H. GilmanII; Rosa CastilloI; Victor TsangIII; Joy BrandtIII; Angela GuevaraI; Hernan SanabriaI; Manuela VerasteguiI; Charles SterlingIV; Elba MirandaI

IThe Universidad Peruana Cayetano Heredia
IIThe Johns Hopkins University
IIIThe Centers for Disease Control
IVThe University of Arizona

Address for Correspondence

 

 


SUMMARY

Sera from patients infected with Taenia solium, Hymenolepis nana and Echinococcus granulosus were tested against homologous and heterologous parasite antigens using an ELISA assay, and a high degree of cross-reactivity was verified. To identify polypeptides responsible for this cross reactivity, the Enzyme Linked Immunoelectro Transfer Blot (EITB) was used.
Sera from infected patients with T.solium, H.nana, and E.granulosus were assessed against crude, ammonium sulphate precipitated (TSASP), and lentil-lectin purified antigens of T.solium and crude antigens of.H.nana and E.granulosus.
Several bands, recognized by sera from patients with T.solium, H.nana, and E.granulosus infections, were common to either two or all three cestodes. Unique reactive bands in H.nana were noted at 49 and 66 K-Da and in E.granulosus at 17-21 K-Da and at 27-32 K-Da. In the crude cysticercosis extract, a specific non glycoprotein band was present at 61—67 K-Da in addiction to specific glycoprotein bands of 50, 42, 24, 21, 18, 14, and 13 K-Da.
None of the sera from patients with H.nana or E.granulosus infection cross reacted with these seven glycoprotein bands considered specific for T.solium infection.

Keywords: Taenia solium; Cysticercus; antigens; Hymenolepis nana; Echinococcus granulosus; Hydatid Cysts; SDS-PAGE; Electrophoresis; Immunoblotting.


RESUMO

Soros de pacientes infectados com Taenia solium, Hymenolepis nana e Echinococcus granulosus foram testados contra antígenos parasitários homólogos e heterólogos usando o teste de ELISA e foi verificado alto grau de reatividade cruzada. Para identificar os polipetídeos responsáveis por esta reatividade cruzada foi utilizado o teste "Enzyme Linked Immunoelectro Transfer Blot (EITB)".
Soros de pacientes infectados por T.solium, H.nana, e E.granulosus foram colocados em contato com precipitado de sulfato de amônia e antígenos não purificados de T.solium e os de H.nana e E.granulosus.
Várias bandas reconhecidas pelos soros de pacientes com infecção por T.solium, H.nana e E.granulosus foram comuns a dois ou três destes cestódeos. Uma única banda foi notada em H.nana a 49 e 66K-Da e no E.granulosus a 17-21 K-Da e 27-32 K-Da. No extrato não purificado de cisticercose uma banda específica não glicoproteica estava presente a 61-67 K-Da além das bandas de glicoproteínas específicas de 50, 42, 24, 21, 18, 14 e 13 K-Da. Nenhum destes soros de pacientes com infecção por H.nana ou E.granulosus reagiu de forma cruzada com estas sete bandas de glicoproteína consideradas específicas à infecção por T.solium.


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 

 

ACKNOWLEDGMENTS

This work was supported by the Consejo Nacional de Ciencia y Tecnologia (CONCYTEC), Peru., The International Development Research Center (IDRC), Canada., and the PSTC No.7284 Grants.

 

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Address for Correspondence:
MSc. Teresa Montenegro
Depto. Microbiologia, Universidad Peruana Cayetano Heredia
Av. Honorio Delgado nº430
Urb. Ingenieria, San Martin de Porras
Casilla Postal 4314, Lima-Peru

Recebido para publicação em 31/08/1993
Aceito para publicação em 04/05/1994.

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