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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.36 no.5 São Paulo Sept./Oct. 1994

http://dx.doi.org/10.1590/S0036-46651994000500002 

SEROLOGIA

 

Aspectos imunológicos do sistema enzimático fenoloxidase de Schistosoma mansoni

 

Immunological aspects of the phenol oxidase enzymatic system of Schistosoma mansoni

 

 

João Tadeu Ribeiro-PaesI; Paulo Inácio da CostaI; Duarte da SilvaI; Léo Roberto BarthII; Vanderlei RodriguesI

IDepto. de Parasitologia, Microbiologia e Imunologia - Faculdade de Medicina de Ribeirão Preto - Universidade de São Paulo
IIDepto. de Pediatria - Faculdade de Medicina de Ribeirão Preto - Univ. de São Paulo

Endereço para correspondência

 

 


RESUMO

O sistema enzimático fenoloxidase (EC 1.10.3.1, EC 1.10.3.2) está amplamente distribuido entre os seres vivos, tendo sido descrito em diferentes espécies do reino animal e vegetal. Apesar de desempenhar um papel fundamental na formação da cápsula ou parede dos ovos de trematódeos, o sistema enzimático fenoloxidase (PO) tem sido pouco estudado nesses organismos. No presente trabalho são apresentados os resultados iniciais de imunizações de coelhos contra PO de fêmeas adultas de S. mansoni e tirosinase de cogumelo (Sigma). As análises imunológicas, realizadas através de imunodifusão dupla (teste de Ouchterlony) e imunoeletroforese, revelaram identidade imunitária parcial entre a PO de machos e fêmeas. Não se observou reação cruzada entre os antissoros de coelhos imunizados contra PO e aqueles com tirosinase, indicando que, embora os sítios catalíticos de ambas as enzimas devam ser semelhantes, já que atuam sobre os mesmos substratos, os determinantes antigênicos devem ser diferentes. Os resultados descritos no presente trabalho representam um primeiro passo no sentido da purificação das isoenzimas da fenoloxidase e sua posterior utilização ao estudo dos mecanismos moleculares envolvidos na esclerotização da parede dos ovos de S. mansoni.

Unitermos: Schistosoma mansoni; Fenoloxidase; Parede dos ovos.


SUMMARY

The phenol oxidase enzymatic system (EC 1.10.3.1, EC 1.10.3.2) is widespread in different species of the animal and vegetal kingdom. Despite its importance in the eggshell formation of the trematodes phenol oxidase (PO) has been little studied in these organisms, mainly in S. mansoni. This report presents the initial results concerning the immunization of rabbits with PO of S. mansoni and mushroom tyrosinase. The immunological analysis done by means of double immu-nodifusion (Ouchterlony) and immunoelectrophoresis techniques revealed some immunological identity between the PO of males and females. It was not seen cross reaction between the antisera against PO and tyrosinase, what suggests that the antigenic determinants of both enzymes are different in spite of their catalytic sites being similar, since they act over the same substrate. The results reported here represent a first step in way to obtain the PO isoenzymes in their pure form and should open new insights for further studies on the molecular mechanisms involved in the sclerotization process of the S. mansoni eggshell.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 


AGRADECIMENTOS

Agradecemos a Olinda Mara B. Trevilato e Vânia Fernandez pela contribuição técnica e ao Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) pelo suporte financeiro.

 

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Endereço para correspondência:
Dr. Vanderlei Rodrigues
Depto. de Parasitologia,Microbiologia e Imunologia - Faculdade de Medicina de Ribeirão Preto
14049-900 Ribeirão Preto, São Paulo, SP, Brasil

Recebido para publicação em 20/12/1993.
Aceito para publicação em 08/07/1994.

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