Chicken as potential contamination source of Campylocacter lari in Iquitos, Peru

Tresierra-Ayala, Alvaro; Bendayan, Maria Elena; Bernuy, Alfonso; Pereyra, Gustavo; Fernandez, Heriberto

doi:10.1590/S0036-46651994000600004

Abstracts

In order to know the importance of chicken as natural reservoir of Campylobacter lari in Iquitos, Peru; samples were obtained by cloacal swabs from 200 chickens and immediately placed into a semisolid enrichment medium; these were streaked on modified Skirrow Agar. The organism was isolated from 21 (10.5%) samples, corresponding 58.8% to biovar I and 41.2% to biovar II (Lior scheme). The results provide evidence that chicken appear to be prominent reservoirs of Campylobacter lari in Iquitos.

Campylobacter lari; Chicken; Reservoir

Com o objetivo de conhecer a importância dos frangos como reservatório natural de Campylobacter lari na cidade de Iquitos, Perú, foram estudadas amostras cloacais obtidas de 200 aves. Cada amostra foi semeada em meio de enriquecimento semi-sólido e no ágar de Skirrow modificado. C. lari foi isolado em 21 (10,5%) amostras. Destes, 58,8% corresponderam ao biotipo I e 41,2% ao biotipo II do esquema de Lior. Os resultados obtidos sugerem que os frangos podem ser um importante reservatório de C. lari em Iquitos, Perú.

MICROBIOLOGY

Chicken as potential contamination source of Campylocacter lari in Iquitos, Peru

Frangos como fonte potencial de contaminação por Campylobacter lari em Iquitos, Peru

Alvaro Tresierra-Ayala^I; Maria Elena Bendayan^I; Alfonso Bernuy^I; Gustavo Pereyra^I; Heriberto Fernandez^II

^IDepartment of Microbiology. Universidad Nacional de la Amazonia Peruana. P.O. Box 751. Iquitos-Peru

^IIInstitute of Clinical Microbiology. Universidad Austral de Chile. P.O. Box 567. Valdivia - Chile

^{Correspondence to} Correspondence to: Prof. Alvaro Tresierra Ayala Casilla Postal 751 Iquitos - Peru

SUMMARY

In order to know the importance of chicken as natural reservoir of Campylobacter lari in Iquitos, Peru; samples were obtained by cloacal swabs from 200 chickens and immediately placed into a semisolid enrichment medium; these were streaked on modified Skirrow Agar. The organism was isolated from 21 (10.5%) samples, corresponding 58.8% to biovar I and 41.2% to biovar II (Lior scheme). The results provide evidence that chicken appear to be prominent reservoirs of Campylobacter lari in Iquitos.

Keywords:Campylobacter lari; Chicken; Reservoir.

RESUMO

Com o objetivo de conhecer a importância dos frangos como reservatório natural de Campylobacter lari na cidade de Iquitos, Perú, foram estudadas amostras cloacais obtidas de 200 aves. Cada amostra foi semeada em meio de enriquecimento semi-sólido e no ágar de Skirrow modificado. C. lari foi isolado em 21 (10,5%) amostras. Destes, 58,8% corresponderam ao biotipo I e 41,2% ao biotipo II do esquema de Lior. Os resultados obtidos sugerem que os frangos podem ser um importante reservatório de C. lari em Iquitos, Perú.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 03/01/1994.

Aceito para publicação em 25/05/1994.

Financial support: Grant of Investig. Office - U.N.A.P. and CONCYTEC. Grant S-92-05 DID-UACH.

1. BENJAMIN, J.; LEAPER, S.; OWEN, R.J. & SKIRROW, M.B. - Description of Campylobacter laridis, a new species comprising the nalidixic acid resistant thermophilic Campylobacter (NARTC) group. Curr. Microbiol., 8: 231-238, 1983.
2. BLACK, R.; LOPEZ DE ROMAŃA, G.; BROWN, K. et al. - Incidence and etiology of infantile diarrhea and major routes of transmission in Huascar, Peru. Amer. J. Epidem., 129: 785-799, 1989.
3. BLASER, M.J.; TAYLOR, D.N. & FELDMAN, R.A. - Epidemiology of Campylobacter jejuni infections. Epidem. Rev., 5: 157-176, 1983.
4. BORCZYC, A.; THOMPSON, S.; SMITH, D. & LIOR, H. - Water-borne outbreak of Campylobacter laridis-associated gastroenteritis. Lancet, 1: 164-165, 1987.
5. BUTZLER, J.P. & OOSTEROM, J. - Campylobacter: pathogenicity and significance in foods. Int. J. Food Microbiol., 12: 1-8, 1991.
6. CARLSON, J. - 162 cases of Campylobacter and Salmonella as a result of a waterborne outbreak in a nuclear reactor station. Ontario Dis. Surveill. Rep., 6: 544-545, 1985.
7. FERNANDEZ, H. - Thermophilic species of Campylobacter: bacteriological, epidemiological and pathogenical aspects. Săo Paulo, 1983. (Doctoral Thesis - School of Medicine of Săo Paulo - Escola Paulista de Medicina).
8. FERNANDEZ, H. - Increase of Campylobacter isolation rates using an enrichment medium. Rev. Microbiol. (S. Paulo), 23: 5-7, 1992.
9. FERNANDEZ, H. - Thermotolerant Campylobacter species associated with human diarrhea in Latin America. Cienc. e Cult. (J. Braz. Ass. Adv. Sci)., 44: 39-43, 1992.
10. FERNANDEZ, H.; LANDSKRON, E.; FIGUEROA, G.; GESCHE, W. & MONTEFUSCO, A. - Campylobacter laridis: first clinical isolation and identification of reservoir in Chile. Rev. méd. Chile., 118: 699-701, 1990.
11. GOOSSENS, H. & BUTZLER, J.P. - Isolation and identification of Campylobacter spp. In: NACHAMKIN, I.; BLASER, J.M. & TOMPKINS, L.S., ed. - Campylobacter jejuni. Current status and future trends. Washington, American Society for Microbiology, 1992.
12. GRADOS, O.; BRAVO, N.; BLACK, R.E. & BUTZLER, J.P. - Paediatric Campylobacter diarrhoea from household exposure to live chickens in Lima, Peru. Bull. Wld. Hlth. Org., 66: 369-374, 1988.
13. GREENBERG, B.L.; SACK, R.B.; SALAZAR-LINDO, R.E. et al. - Measles-associated diarrhea in hospitalizad children in Lima, Peru: pathogenic agents and impact on growth. J. infect. Dis., 163: 495-502, 1991.
14. HODGE, D.S.; BORCZYK, A. & WAT, L.L. - Evaluation of the indoxil acetate hydrolysis test for the differentiation of campylobacters. J. clin. Microbiol., 28: 1482-1483, 1990.
15. HUTCHINSON, D.N. & BOLTON, F.J. - An improved blood-free medium for isolation of Campylobacter jejuni from faecal specimens. J. clin. Path., 37: 956-957, 1984.
16. KASRAZADEH, M. & GENIGEORGIS, C. - Origin and prevalence of Campylobacter jejuni in ducks and duck meat at the farm and processing plant level. J. Food protect., 50: 321-326, 1987.
17. KWIATEK, K.; WOJTON, B. & STERN, N.J. - Prevalence and distribution of Campylobacter spp. on poultry and selected meat carcasses in Poland. J. Food protect., 53: 321-326, 1990.
18. LIOR, H. - New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis". J. clin. Microbiol., 20: 636-640, 1984.
19. LUECHTEFELD, N.; CAMBRE, R. & WANG, W. - Isolation of Campylobacter fetus subsp. jejuni from zoo animals. J. Amer. Med. vet. Ass., 179: 1119-1122, 1981.
20. MEGRAUD, F.; CHEVRIER, D.; DESPLACES, N.; SEDALLIAN, A. & GUESDON, J.L. - Urease-positive thermophilic Campylobacter (C. laridis variant) isolated from an appendix and from human feces. J. clin. Microbiol., 26: 1050-1051, 1988.
21. SHANE, S.M. - The significance of Campylobacter jejuni in poultry: a review. Avian Path., 21: 189-213, 1992.
22. SIMOR, A. & WILCOX, L. - Enteritis associated with Campylobacter laridis. J. clin. Microbiol., 25: 10-12, 1987.
23. SKIRROW, M.B. & BENJAMIN, J. - "1001" campylobacters: cultural characteristics of intestinal campylobacters from man and animals. J. Hyg., 85: 427-442, 1980.
24. TAUXE, R.V.; PATTON, C.M.; EDMONDS, P. et al. - Illness associated with Campylobacter laridis, a new recognized Campylobacter species. J. clin. Microbiol., 21: 222-225, 1985.
25. VON GRAEVENITZ, A. - Revised nomenclature of Campylobacter laridis, Enterobacter intermedium and "Flavobacterium brachiophila". Int. J. system. Bact., 40: 211, 1990.

Correspondence to:

Prof. Alvaro Tresierra Ayala

Casilla Postal 751

Iquitos - Peru

Publication Dates

Publication in this collection
05 July 2006
Date of issue
Dec 1994

History

Accepted
25 May 1994
Received
03 Jan 1994

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BENJAMIN, J.; LEAPER, S.; OWEN, R.J. & SKIRROW, M.B. - Description of Campylobacter laridis, a new species comprising the nalidixic acid resistant thermophilic Campylobacter (NARTC) group. Curr. Microbiol., 8: 231-238, 1983.

[2] 2. BLACK, R.; LOPEZ DE ROMAŃA, G.; BROWN, K. et al. - Incidence and etiology of infantile diarrhea and major routes of transmission in Huascar, Peru. Amer. J. Epidem., 129: 785-799, 1989.

[3] 3. BLASER, M.J.; TAYLOR, D.N. & FELDMAN, R.A. - Epidemiology of Campylobacter jejuni infections. Epidem. Rev., 5: 157-176, 1983.

[4] 4. BORCZYC, A.; THOMPSON, S.; SMITH, D. & LIOR, H. - Water-borne outbreak of Campylobacter laridis-associated gastroenteritis. Lancet, 1: 164-165, 1987.

[5] 5. BUTZLER, J.P. & OOSTEROM, J. - Campylobacter: pathogenicity and significance in foods. Int. J. Food Microbiol., 12: 1-8, 1991.

[6] 6. CARLSON, J. - 162 cases of Campylobacter and Salmonella as a result of a waterborne outbreak in a nuclear reactor station. Ontario Dis. Surveill. Rep., 6: 544-545, 1985.

[7] 7. FERNANDEZ, H. - Thermophilic species of Campylobacter: bacteriological, epidemiological and pathogenical aspects. Săo Paulo, 1983. (Doctoral Thesis - School of Medicine of Săo Paulo - Escola Paulista de Medicina).

[8] 8. FERNANDEZ, H. - Increase of Campylobacter isolation rates using an enrichment medium. Rev. Microbiol. (S. Paulo), 23: 5-7, 1992.

[9] 9. FERNANDEZ, H. - Thermotolerant Campylobacter species associated with human diarrhea in Latin America. Cienc. e Cult. (J. Braz. Ass. Adv. Sci)., 44: 39-43, 1992.

[10] 10. FERNANDEZ, H.; LANDSKRON, E.; FIGUEROA, G.; GESCHE, W. & MONTEFUSCO, A. - Campylobacter laridis: first clinical isolation and identification of reservoir in Chile. Rev. méd. Chile., 118: 699-701, 1990.

[11] 11. GOOSSENS, H. & BUTZLER, J.P. - Isolation and identification of Campylobacter spp. In: NACHAMKIN, I.; BLASER, J.M. & TOMPKINS, L.S., ed. - Campylobacter jejuni. Current status and future trends. Washington, American Society for Microbiology, 1992.

[12] 12. GRADOS, O.; BRAVO, N.; BLACK, R.E. & BUTZLER, J.P. - Paediatric Campylobacter diarrhoea from household exposure to live chickens in Lima, Peru. Bull. Wld. Hlth. Org., 66: 369-374, 1988.

[13] 13. GREENBERG, B.L.; SACK, R.B.; SALAZAR-LINDO, R.E. et al. - Measles-associated diarrhea in hospitalizad children in Lima, Peru: pathogenic agents and impact on growth. J. infect. Dis., 163: 495-502, 1991.

[14] 14. HODGE, D.S.; BORCZYK, A. & WAT, L.L. - Evaluation of the indoxil acetate hydrolysis test for the differentiation of campylobacters. J. clin. Microbiol., 28: 1482-1483, 1990.

[15] 15. HUTCHINSON, D.N. & BOLTON, F.J. - An improved blood-free medium for isolation of Campylobacter jejuni from faecal specimens. J. clin. Path., 37: 956-957, 1984.

[16] 16. KASRAZADEH, M. & GENIGEORGIS, C. - Origin and prevalence of Campylobacter jejuni in ducks and duck meat at the farm and processing plant level. J. Food protect., 50: 321-326, 1987.

[17] 17. KWIATEK, K.; WOJTON, B. & STERN, N.J. - Prevalence and distribution of Campylobacter spp. on poultry and selected meat carcasses in Poland. J. Food protect., 53: 321-326, 1990.

[18] 18. LIOR, H. - New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis". J. clin. Microbiol., 20: 636-640, 1984.

[19] 19. LUECHTEFELD, N.; CAMBRE, R. & WANG, W. - Isolation of Campylobacter fetus subsp. jejuni from zoo animals. J. Amer. Med. vet. Ass., 179: 1119-1122, 1981.

[20] 20. MEGRAUD, F.; CHEVRIER, D.; DESPLACES, N.; SEDALLIAN, A. & GUESDON, J.L. - Urease-positive thermophilic Campylobacter (C. laridis variant) isolated from an appendix and from human feces. J. clin. Microbiol., 26: 1050-1051, 1988.

[21] 21. SHANE, S.M. - The significance of Campylobacter jejuni in poultry: a review. Avian Path., 21: 189-213, 1992.

[22] 22. SIMOR, A. & WILCOX, L. - Enteritis associated with Campylobacter laridis. J. clin. Microbiol., 25: 10-12, 1987.

[23] 23. SKIRROW, M.B. & BENJAMIN, J. - "1001" campylobacters: cultural characteristics of intestinal campylobacters from man and animals. J. Hyg., 85: 427-442, 1980.

[24] 24. TAUXE, R.V.; PATTON, C.M.; EDMONDS, P. et al. - Illness associated with Campylobacter laridis, a new recognized Campylobacter species. J. clin. Microbiol., 21: 222-225, 1985.

[25] 25. VON GRAEVENITZ, A. - Revised nomenclature of Campylobacter laridis, Enterobacter intermedium and "Flavobacterium brachiophila". Int. J. system. Bact., 40: 211, 1990.

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