Haemolytic activity of Actinobacillus actinomycetemcomitans strains on different blood types

Avila-Campos, Mario Julio

doi:10.1590/S0036-46651995000300006

Abstracts

Haemolytic activity of sixty nine Actinobacillus actinomycetemcomitans strains on different animal and human blood types was examined by using a trypticase soy agar supplemented with yeast extract (0.5%). Blood types used were: rabbit, sheep and human (A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+; AB, Rh- groups). Plates were inoculated and, incubated in microaerophilic conditions, at 37ºC, for 48 h. The haemolytic activity of the tested strains was characterized as alpha-haemolysis. Only two isolates were not haemolytic on all blood types (2.9%), two strains were haemolytic only on human blood (one strain on AB, Rh+ group and another one on A, Rh+ and AB, Rh+ groups). No specificity between haemolysin produced by the tested strains and blood type was observed.

Actinobacillus actinomycetemcomitans; Haemolysis; Pathogenicity

A atividade hemolítica de 69 cepas de Actinobacillus actinomycetemcomitans foi determinada em diferentes tipos de sangue animal e humano, usando como meio base ágar de soja tripticaseina, suplementado com extrato de levedura (0,5%). Foram utilizados sangue de coelho, carneiro e humano (grupos A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+ e AB, Rh-). As placas foram inoculadas e, incubadas em condições de microaerofilia, a 37ºC, por 48 h. A atividade hemolítica das cepas testadas foi caracterizada como alfa-hemólise. Somente dois (2,9%) isolados não hemolisaram todos os tipos sanguíneos, duas cepas hemolisaram somente sangue humano (uma o grupo AB, Rh+ e outra os grupos A, Rh+ e AB, Rh+). Não foi observada alguma especificidade entre as hemolisinas produzidas e os tipos de sangue utilizados.

MICROBIOLOGY

Haemolytic activity of Actinobacillus actinomycetemcomitans strains on different blood types

Atividade hemolítica de cepas de Actinobacillus actinomycetemcomitans em diferentes tipos sanguíneos

Mario Julio Avila-Campos

^{Correspondence to} Correspondence to: Dr. Mario Julio Avila-Campos Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo 05508-900 São Paulo, SP, Brazil E-mail: Mariojac@biomed.ICB2.usp.br

SUMMARY

Haemolytic activity of sixty nine Actinobacillus actinomycetemcomitans strains on different animal and human blood types was examined by using a trypticase soy agar supplemented with yeast extract (0.5%). Blood types used were: rabbit, sheep and human (A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+; AB, Rh- groups). Plates were inoculated and, incubated in microaerophilic conditions, at 37ºC, for 48 h. The haemolytic activity of the tested strains was characterized as alpha-haemolysis. Only two isolates were not haemolytic on all blood types (2.9%), two strains were haemolytic only on human blood (one strain on AB, Rh+ group and another one on A, Rh+ and AB, Rh+ groups). No specificity between haemolysin produced by the tested strains and blood type was observed.

Keywords:Actinobacillus actinomycetemcomitans; Haemolysis; Pathogenicity.

RESUMO

A atividade hemolítica de 69 cepas de Actinobacillus actinomycetemcomitans foi determinada em diferentes tipos de sangue animal e humano, usando como meio base ágar de soja tripticaseina, suplementado com extrato de levedura (0,5%). Foram utilizados sangue de coelho, carneiro e humano (grupos A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+ e AB, Rh-). As placas foram inoculadas e, incubadas em condições de microaerofilia, a 37ºC, por 48 h. A atividade hemolítica das cepas testadas foi caracterizada como alfa-hemólise. Somente dois (2,9%) isolados não hemolisaram todos os tipos sanguíneos, duas cepas hemolisaram somente sangue humano (uma o grupo AB, Rh+ e outra os grupos A, Rh+ e AB, Rh+). Não foi observada alguma especificidade entre as hemolisinas produzidas e os tipos de sangue utilizados.

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ACKNOWLEDGEMENTS

I wish to express my sincere gratitude to Dra. Maria Auxiliadora Roque de Carvalho and Dr. Flávio Zelante for their critical reading, and Andemir da Silva and João Paulo Ribeiro for their technical help.

This work was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Proc. 93/0056-0.

Recebido para publicação em 12/07/1994.

Aceito para publicação em 29/03/1995.

1. ALEXANDER, A.D.- Actinobacillus actinomycetemcomitans. In: BLAIR, J.E.; LENNETTE, E.H. & TRUANT, J.P., ed. Manual of Clinical Microbiology. Bethesda, American Society for Microbiology, 1972. p. 220-222..
2. AVILA-CAMPOS, M.J.; FARIAS, L.M.; CARVALHO, M.A.R. et al.- Atividade hemolítica de Actinobacillus actinomycetemcomitans. Rev. Microbiol., 19: 262-265, 1988.
3. FARIAS, L.M.; CARVALHO, M.A.R.; DAMASCENO, C.A.V. et al.- Caracterizaçăo morfológica, bioquímica e fisiológica de Actinobacillus actinomycetemcomitans isoladas de lesőes periodontais humanas. Rev. Microbiol., 17: 296-306, 1986.
4. HILMAN, J.D.; MAIDEN, M.F.J.; PFALLER, S.P. et al. - Characterization of hemolytic bacteria in subgingival plaque. J. Periodont. Res., 28: 173-179, 1993.
5. REIDER, J. & WHEAT, J. - Endocarditis caused by Actinobacillus actinomycetemcomitans. Sth. med. J. (Bgham, Ala.), 72: 1219-1224, 1979.
6. ROGOLSKY, M. - Nonteric toxin of Staphylococcus aureus. Microbiol. Rev., 43: 320-360, 1979.
7. SLOTS, J.; REYNOLDS, H.S. & GENCO, R.J. - Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect. Immun., 29: 1013-1020, 1980.
8. SLOTS, J.- Selective medium for Actinobacillus actinomycetemcomitans. J. clin. Microbiol., 15: 606-609, 1982.
9. SLOTS, J.- Salient biochemical characters of Actinobacillus actinomycetemcomitans. Arch. Microbiol., 131: 60-67, 1982.
10. TSAI, C.C.; Mc ARTHUR, W.; BAEHNI, P. et al. - Extraction and partial characterization of a leukotoxin from a plaque derived Gram-negative microorganisms. Infect. Immun., 25: 427-439, 1979.
11. TSAI, C.C.; SHENKER, B.J.; DI RIENZO, J.M. et al. - Extraction and isolation of a leukotoxin from Actinobacillus actinomycetemcomitans with polymyxin B. Infect. Immun., 43: 700-705, 1984.
12. WELCH, R.A. & FALKOW, S. - Characterization of Escherichia coli hemolysin conferring quantitative virulence. Infect. Immun., 43: 156-160, 1984.
13. ZAMBON, J.J.; CHRISTERSSON, L.A. & SLOTS, J. - Actinobacillus actinomycetemcomitans in human periodontal disease. J. Periodont., 54: 707-711, 1983.

Correspondence to:

Dr. Mario Julio Avila-Campos

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo

05508-900 São Paulo, SP, Brazil

E-mail:

Mariojac@biomed.ICB2.usp.br

Publication Dates

Publication in this collection
21 Sept 2006
Date of issue
June 1995

History

Received
12 July 1994
Accepted
29 Mar 1995

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALEXANDER, A.D.- Actinobacillus actinomycetemcomitans. In: BLAIR, J.E.; LENNETTE, E.H. & TRUANT, J.P., ed. Manual of Clinical Microbiology. Bethesda, American Society for Microbiology, 1972. p. 220-222..

[2] 2. AVILA-CAMPOS, M.J.; FARIAS, L.M.; CARVALHO, M.A.R. et al.- Atividade hemolítica de Actinobacillus actinomycetemcomitans. Rev. Microbiol., 19: 262-265, 1988.

[3] 3. FARIAS, L.M.; CARVALHO, M.A.R.; DAMASCENO, C.A.V. et al.- Caracterizaçăo morfológica, bioquímica e fisiológica de Actinobacillus actinomycetemcomitans isoladas de lesőes periodontais humanas. Rev. Microbiol., 17: 296-306, 1986.

[4] 4. HILMAN, J.D.; MAIDEN, M.F.J.; PFALLER, S.P. et al. - Characterization of hemolytic bacteria in subgingival plaque. J. Periodont. Res., 28: 173-179, 1993.

[5] 5. REIDER, J. & WHEAT, J. - Endocarditis caused by Actinobacillus actinomycetemcomitans. Sth. med. J. (Bgham, Ala.), 72: 1219-1224, 1979.

[6] 6. ROGOLSKY, M. - Nonteric toxin of Staphylococcus aureus. Microbiol. Rev., 43: 320-360, 1979.

[7] 7. SLOTS, J.; REYNOLDS, H.S. & GENCO, R.J. - Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect. Immun., 29: 1013-1020, 1980.

[8] 8. SLOTS, J.- Selective medium for Actinobacillus actinomycetemcomitans. J. clin. Microbiol., 15: 606-609, 1982.

[9] 9. SLOTS, J.- Salient biochemical characters of Actinobacillus actinomycetemcomitans. Arch. Microbiol., 131: 60-67, 1982.

[10] 10. TSAI, C.C.; Mc ARTHUR, W.; BAEHNI, P. et al. - Extraction and partial characterization of a leukotoxin from a plaque derived Gram-negative microorganisms. Infect. Immun., 25: 427-439, 1979.

[11] 11. TSAI, C.C.; SHENKER, B.J.; DI RIENZO, J.M. et al. - Extraction and isolation of a leukotoxin from Actinobacillus actinomycetemcomitans with polymyxin B. Infect. Immun., 43: 700-705, 1984.

[12] 12. WELCH, R.A. & FALKOW, S. - Characterization of Escherichia coli hemolysin conferring quantitative virulence. Infect. Immun., 43: 156-160, 1984.

[13] 13. ZAMBON, J.J.; CHRISTERSSON, L.A. & SLOTS, J. - Actinobacillus actinomycetemcomitans in human periodontal disease. J. Periodont., 54: 707-711, 1983.

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