Egg excretion in the initial phase of experimental murine schistosomiasis mansoni: stability and association with worm burden

Rocha, Regina Lunardi; Rocha, Manoel Otávio da Costa; Pedroso, Ênio Roberto Pietra; Colosimo, Enrico Antônio; Coelho, Paulo Marcos Zech

doi:10.1590/S0036-46651995000400007

Abstracts

Stability of faecal egg excretion and correlation with results related to worm burden at the initial phase of schistosomiasis mansoni were observed in two groups of mice infected with different Schistosoma mansoni cercarial burdens, by means of analysis of quantitative parasitological studies and schistosome counts after perfusion. Thus, it may be stated that few quantitative parasitological stool examinations could be sufficient to express the infection intensity at the initial phase, on the same grounds that it was already demonstrated at the chronic phase. Furthermore, it is confirmed that the use of the number of eggs passed in the faeces as a tool to estimate the worm burden at the initial phase of schistosome infection is adequate.

Schistosomiasis; Egg excretion; Stability; Worm burden

Através da análise de exames parasitológicos quantitativos seriados e da contagem de esquistossomos após perfusão em dois grupos de camundongos infectados com diferentes cargas de cercárias de Schistosoma mansoni, verificou-se a existência da estabilidade da eliminação de ovos e sua correlação com a carga parasitária na fase inicial da esquistossomose mansoni. Deste modo, pode-se afirmar que poucos exames parasitológicos de fezes quantitativos podem ser suficientes para traduzir a intensidade da infecção também na fase inicial , à semelhança do já demonstrado para a crônica. Além disto, comprova-se a adequação do uso do número de ovos eliminados nas fezes como expressão da carga parasitária na fase inicial da infecção esquistossomótica.

SCHISTOSOMIASIS

Egg excretion in the initial phase of experimental murine schistosomiasis mansoni: stability and association with worm burden

Esquistossomose mansoni experimental murina: estabilidade da eliminação dos ovos nas fezes e sua associação com a carga parasitária na fase inicial da infecção

Regina Lunardi Rocha^I; Manoel Otávio da Costa Rocha^I; Ênio Roberto Pietra Pedroso^I; Enrico Antônio Colosimo^II; Paulo Marcos Zech Coelho^III

^IPostgraduate Course of Tropical Medicine, School of Medicine, Federal University of Minas Gerais, Av. Prof. Alfredo Balena, 190, 30130-100, Belo Horizonte, Minas Gerais - Brazil

^IIDepartment of Statistics, Institute of Exact Sciences, Federal University of Minas Gerais, Brazil

^IIIDepartment of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais - Brasil

SUMMARY

Stability of faecal egg excretion and correlation with results related to worm burden at the initial phase of schistosomiasis mansoni were observed in two groups of mice infected with different Schistosoma mansoni cercarial burdens, by means of analysis of quantitative parasitological studies and schistosome counts after perfusion. Thus, it may be stated that few quantitative parasitological stool examinations could be sufficient to express the infection intensity at the initial phase, on the same grounds that it was already demonstrated at the chronic phase. Furthermore, it is confirmed that the use of the number of eggs passed in the faeces as a tool to estimate the worm burden at the initial phase of schistosome infection is adequate.

Keywords: Schistosomiasis; Egg excretion; Stability; Worm burden.

RESUMO

Através da análise de exames parasitológicos quantitativos seriados e da contagem de esquistossomos após perfusão em dois grupos de camundongos infectados com diferentes cargas de cercárias de Schistosoma mansoni, verificou-se a existência da estabilidade da eliminação de ovos e sua correlação com a carga parasitária na fase inicial da esquistossomose mansoni.

Deste modo, pode-se afirmar que poucos exames parasitológicos de fezes quantitativos podem ser suficientes para traduzir a intensidade da infecção também na fase inicial , à semelhança do já demonstrado para a crônica.

Além disto, comprova-se a adequação do uso do número de ovos eliminados nas fezes como expressão da carga parasitária na fase inicial da infecção esquistossomótica.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 03/03/1995

Aceito para publicação cm 18/05/1995

1. ARAP SIONGOK, T.K.; MAHMOUD, A.A.F.; OUMA, J.H. et al. - Morbidity in schistosomiasis mansoni in relation to intensity of infection: study of a community in Machacos, Kenya. Amer. J. trop. Med. Hyg., 25: 273-284, 1976.
2. BARRETO, M.L.; SILVA, J.T.F.; MOTT, K.E. & LEHMAN Jr., J.S. - Stability of faecal egg excretion in Schistosoma mansoni infection. Trans. roy. Soc. trop. Med. Hyg., 72: 181-187, 1978.
3. CHEEVER, A.W. - A quantitative post-mortem study of schistosomiasis mansoni in man. Amer. J. trop. Med. Hyg., 17: 38-64, 1968.
4. CHEEVER, A.W.; MACEDONIA, J.G.; MOSHMANN, J.E. & CHEEVER, E.A. - Kinetics of egg production and egg excretion by Schistosoma mansoni and Schistosoma japonicum in mice infected with a single pair of worms. Amer. J. trop. Med. Hyg., 50: 281-295, 1994a.
5. CHEEVER, A.W.; MOSHMANN, J.E.; DEB, S.; CHEEVER, E.A. & DUVALL, R.H. - Natural history of Schistosoma mansoni in mice: egg production, egg passage in the feces, and contribution of host and parasite dealh to changes in worm numbers. Amer. J. trop. Med. Hyg., 50: 269-280, 1994b.
6. CLARK, W.D.; COX, P.M.; RATNER, L.H. & CORREA-CORONAS, R. - Acute schistosomiasis mansoni in 10 boys. An outbreak in Caguas, Puerto Rico. Ann. intern. Med., 73: 379-385, 1970.
7. COOK, J.A.; BAKER, S.T.; WARREN, K.S. & JORDAN, P. - A controlled study of morbidity of schistosomiasis mansoni in St. Lucian children, based on quantitative egg excretion. Amer. J. trop. Med. Hyg., 23: 625-633, 1974.
8. COSTA, M.F.F.L.; ROCHA, R.S. & KATZ, N. - Avaliaçăo da estabilidade na contagem de ovos de Schistosoma mansoni pelo método de Kato-Katz em urna zona endęmica de esquistossomose. Rev. Soc. bras. Med. trop., 17: 7-12, 1984.
9. GAZZINELLI, G.; LAMBERTUCCI, J.R.; KATZ, N. et al. - Immune response during human schistosomiasis mansoni. XI - Immunologic status of patients with acute infections and after treatment. J. Immunol., 135: 2121-2127, 1985.
10. HIATT, R.A.; OTTESEN, E.A.; SOTOMAYOR, Z.R. & LAWLEY, T.J. - Serial observations of circulating immune complexes in patients with acute schistosomiasis. J. infect. Dis., 142: 665-670, 1980.
11. HIATT, R.A.; SOTOMAYOR, Z.R.; SANCHES, G.; ZAMBANA, M. & KNIGHT, W.B. - Factors in the path-ogenesis of acute schistosomiasis mansoni. J. infect. Dis., 139: 659-666, 1979.
12. HUSSAIN, R.; HOFSTETTER, M.; GOLDSTONE, A.; KNIGHT, W.B. & OTTESEN, E.A. - IgE responses in human schistosomiasis. I. Quantitation of specific IgE by radioimmunoassay and correlation of results with skin test and basophil histamine release. Amer. J. trop. Med. Hyg., 132: 1347-1355, 1983.
13. KATZ, N. - Experięncias com quimioterapia em grande escala no controle da esquistossomose no Brasil. Rev. Inst. Med. trop. S. Paulo, 22: 40-51, 1980.
14. KOURA, M. - The relation between egg production and worm burden in experimental schistosomiasis. I - Worm burden. J. Egypt. med. Ass., 53: 598-603, 1970.
15. LAMBERTUCCI, J.R.; PEDROSO, E.R.P.; GRECO, D.B. & ROCHA, M.O.C. - Estabilidade na eliminaçăo de ovos de S. mansoni nas fezes de crianças com esquistossomose mansoni crônica. Rev. Inst. Med. trop. S. Paulo, 25: 67-72, 1983.
16. MILLIKEN, G.A. & JOHNSON, D.E. - Analysis of Messy data. New York, Van Nostrand Reinhold, 1984.
17. OTTESEN, E.A.; POINDEXTER, R.W. & HUSSAIN, R. - Detection, quantitation and specificity of antiparasite IgE antibodies in human schistosomiasis mansoni. Amer. J. trop. Med. Hyg., 30: 1228-1237, 1981.
18. PELLEGRINO, J. & SIQUEIRA, A.F. - Técnica de perfusăo para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.
19. PELLEGRINO, J. & KATZ, N. - Experimental chemotherapy of schistosomiasis mansoni. In: DAWES, B. Advances in Parasitology. London, Academic Press, 1968. v.6, p. 233-290.
20. RABELLO, A.L.T. - O exame parasitológico de fezes, a biópsia retal e o ensaio imunoenzimático no diagnóstico da esquistossomose mansoni humana. Belo Horizonte, 1990. (Tese de Mestrado - Faculdade de Medicina da Universidade Federal de Minas Gerais).
21. SCOTT, J.A. - The regularity of egg output of helminth infections, with special reference to S. mansoni. Amer. J. trop. Med. Hyg., 27: 155-157, 1938.
22. SMITH, D.H.; WARREN, K.S. & MAHMOUD, A.A.F. - Morbidity in schistosomiasis mansoni in relation to intensity of infection: study of a community in Kisumu, Kenya. Amer. J. trop. Med. Hyg., 28: 220-229, 1979.
23. SNEDECOR, G.A. & COCHRAN, W.G. - Statistical methods. Iowa, The IOWA State University Press, 1980.

Publication Dates

Publication in this collection
21 Sept 2006
Date of issue
Aug 1995

History

Accepted
18 May 1995
Received
03 Mar 1995

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ARAP SIONGOK, T.K.; MAHMOUD, A.A.F.; OUMA, J.H. et al. - Morbidity in schistosomiasis mansoni in relation to intensity of infection: study of a community in Machacos, Kenya. Amer. J. trop. Med. Hyg., 25: 273-284, 1976.

[2] 2. BARRETO, M.L.; SILVA, J.T.F.; MOTT, K.E. & LEHMAN Jr., J.S. - Stability of faecal egg excretion in Schistosoma mansoni infection. Trans. roy. Soc. trop. Med. Hyg., 72: 181-187, 1978.

[3] 3. CHEEVER, A.W. - A quantitative post-mortem study of schistosomiasis mansoni in man. Amer. J. trop. Med. Hyg., 17: 38-64, 1968.

[4] 4. CHEEVER, A.W.; MACEDONIA, J.G.; MOSHMANN, J.E. & CHEEVER, E.A. - Kinetics of egg production and egg excretion by Schistosoma mansoni and Schistosoma japonicum in mice infected with a single pair of worms. Amer. J. trop. Med. Hyg., 50: 281-295, 1994a.

[5] 5. CHEEVER, A.W.; MOSHMANN, J.E.; DEB, S.; CHEEVER, E.A. & DUVALL, R.H. - Natural history of Schistosoma mansoni in mice: egg production, egg passage in the feces, and contribution of host and parasite dealh to changes in worm numbers. Amer. J. trop. Med. Hyg., 50: 269-280, 1994b.

[6] 6. CLARK, W.D.; COX, P.M.; RATNER, L.H. & CORREA-CORONAS, R. - Acute schistosomiasis mansoni in 10 boys. An outbreak in Caguas, Puerto Rico. Ann. intern. Med., 73: 379-385, 1970.

[7] 7. COOK, J.A.; BAKER, S.T.; WARREN, K.S. & JORDAN, P. - A controlled study of morbidity of schistosomiasis mansoni in St. Lucian children, based on quantitative egg excretion. Amer. J. trop. Med. Hyg., 23: 625-633, 1974.

[8] 8. COSTA, M.F.F.L.; ROCHA, R.S. & KATZ, N. - Avaliaçăo da estabilidade na contagem de ovos de Schistosoma mansoni pelo método de Kato-Katz em urna zona endęmica de esquistossomose. Rev. Soc. bras. Med. trop., 17: 7-12, 1984.

[9] 9. GAZZINELLI, G.; LAMBERTUCCI, J.R.; KATZ, N. et al. - Immune response during human schistosomiasis mansoni. XI - Immunologic status of patients with acute infections and after treatment. J. Immunol., 135: 2121-2127, 1985.

[10] 10. HIATT, R.A.; OTTESEN, E.A.; SOTOMAYOR, Z.R. & LAWLEY, T.J. - Serial observations of circulating immune complexes in patients with acute schistosomiasis. J. infect. Dis., 142: 665-670, 1980.

[11] 11. HIATT, R.A.; SOTOMAYOR, Z.R.; SANCHES, G.; ZAMBANA, M. & KNIGHT, W.B. - Factors in the path-ogenesis of acute schistosomiasis mansoni. J. infect. Dis., 139: 659-666, 1979.

[12] 12. HUSSAIN, R.; HOFSTETTER, M.; GOLDSTONE, A.; KNIGHT, W.B. & OTTESEN, E.A. - IgE responses in human schistosomiasis. I. Quantitation of specific IgE by radioimmunoassay and correlation of results with skin test and basophil histamine release. Amer. J. trop. Med. Hyg., 132: 1347-1355, 1983.

[13] 13. KATZ, N. - Experięncias com quimioterapia em grande escala no controle da esquistossomose no Brasil. Rev. Inst. Med. trop. S. Paulo, 22: 40-51, 1980.

[14] 14. KOURA, M. - The relation between egg production and worm burden in experimental schistosomiasis. I - Worm burden. J. Egypt. med. Ass., 53: 598-603, 1970.

[15] 15. LAMBERTUCCI, J.R.; PEDROSO, E.R.P.; GRECO, D.B. & ROCHA, M.O.C. - Estabilidade na eliminaçăo de ovos de S. mansoni nas fezes de crianças com esquistossomose mansoni crônica. Rev. Inst. Med. trop. S. Paulo, 25: 67-72, 1983.

[16] 16. MILLIKEN, G.A. & JOHNSON, D.E. - Analysis of Messy data. New York, Van Nostrand Reinhold, 1984.

[17] 17. OTTESEN, E.A.; POINDEXTER, R.W. & HUSSAIN, R. - Detection, quantitation and specificity of antiparasite IgE antibodies in human schistosomiasis mansoni. Amer. J. trop. Med. Hyg., 30: 1228-1237, 1981.

[18] 18. PELLEGRINO, J. & SIQUEIRA, A.F. - Técnica de perfusăo para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.

[19] 19. PELLEGRINO, J. & KATZ, N. - Experimental chemotherapy of schistosomiasis mansoni. In: DAWES, B. Advances in Parasitology. London, Academic Press, 1968. v.6, p. 233-290.

[20] 20. RABELLO, A.L.T. - O exame parasitológico de fezes, a biópsia retal e o ensaio imunoenzimático no diagnóstico da esquistossomose mansoni humana. Belo Horizonte, 1990. (Tese de Mestrado - Faculdade de Medicina da Universidade Federal de Minas Gerais).

[21] 21. SCOTT, J.A. - The regularity of egg output of helminth infections, with special reference to S. mansoni. Amer. J. trop. Med. Hyg., 27: 155-157, 1938.

[22] 22. SMITH, D.H.; WARREN, K.S. & MAHMOUD, A.A.F. - Morbidity in schistosomiasis mansoni in relation to intensity of infection: study of a community in Kisumu, Kenya. Amer. J. trop. Med. Hyg., 28: 220-229, 1979.

[23] 23. SNEDECOR, G.A. & COCHRAN, W.G. - Statistical methods. Iowa, The IOWA State University Press, 1980.