Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report

Souza, Vanda Akico Ueda Fick de; Sumita, Laura Masami; Otsubo, Mary Eiko S.; Takei, Kioko; Pannuti, Cláudio Sérgio

doi:10.1590/S0036-46651995000400013

Abstracts

A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.

Rubella; ELISA; Antigen

Um método simples de produção de antígeno de vírus da rubéola, por extração com desoxicolato de sódio para aplicação no ensaio imunoenzimático, IMT-ELISA, é apresentado. Este ensaio comparado com ELISA comercial (Enzygnost-Rubella, Behring), no estudo de 108 soros e 118 amostras de papel de filtro apresentou 96,9% (219/226) de concordância e um coeficiente de correlação de 0,90 entre as absorbâncias. Sete amostras apresentaram resultados discordantes, negativos pelo ensaio comercial e positivos pelo IMT-ELISA. Destas, 4 foram testadas por RIH, observando-se positividade em 3.

PRELIMINARY REPORT

Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report

Reação imunoenzimática (ELISA) para detecção de anticorpos contra o vírus da Rubéola: um método simples de produção de antígeno. Nota prévia

Vanda Akico Ueda Fick de Souza^I; Laura Masami Sumita^I; Mary Eiko S. Otsubo^II; Kioko Takei^III; Cláudio Sérgio Pannuti^I

^IDepartamento de Doenças Infecciosas e Parasitárias, Instituto de Medicina Tropical de São Paulo (LIM-52-HC-FMUSP), Faculdade de Medicina da Universidade de São Paulo. São Paulo. Brazil

^IIInstituto Adolfo Lutz. São Paulo. Brazil

^IIIFaculdade de Ciências Farmacêuticas da Universidade de São Paulo. Brazil

^{Correspondence to} Correspondence to: Vanda Akico Ueda Fick de Souza Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar 470 05403-000 São Paulo, SP, Brazil Fax (011) 852.3622

SUMMARY

A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.

Keywords: Rubella; ELISA; Antigen.

RESUMO

Um método simples de produção de antígeno de vírus da rubéola, por extração com desoxicolato de sódio para aplicação no ensaio imunoenzimático, IMT-ELISA, é apresentado. Este ensaio comparado com ELISA comercial (Enzygnost-Rubella, Behring), no estudo de 108 soros e 118 amostras de papel de filtro apresentou 96,9% (219/226) de concordância e um coeficiente de correlação de 0,90 entre as absorbâncias. Sete amostras apresentaram resultados discordantes, negativos pelo ensaio comercial e positivos pelo IMT-ELISA. Destas, 4 foram testadas por RIH, observando-se positividade em 3.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENTS

We thank Dr. Marcos Wolff for the suggestions and for reviewing the manuscript.

Recebido para publicação em 09/01/1995

Aceito para publicação em 06/04/1995

Partially supported by FESIMA. Secretaria de Estado da Saúde de São Paulo, S.P., Brasil

1. ASSAD, F. & LJUNGARS-ESTEVES, K. - Rubella. World impact. Rev. infect. Dis, 7 (suppl.):S29-S36, 1985.
2. BEST, J.M. & O'SHEA, S. - Togaviridae: rubella. In: LENNETTE, E.H.; HALONEN, P. & MURPHY, F.A., ed. Laboratory diagnosis of infectious diseases. Principles and practice. New York, Springer-Verlag, 1988. v.2, p.435-450.
3. FERRARO, M.J.; KALLAS, W.M.; WELCH, K.P. & LAU, A.Y. - Comparison of a new, rapid enzyme immunoassay with a latex agglutination test for qualitative detection of rubella antibodies. J. clin. Microbiol., 25: 1722-1724, 1987.
4. FIELD, P.R.; HO, D.W.T. & CUNNINGHAM, A.L. - Evaluation of rubella immune status by three commercial enzyme-linked immunosorbent assays. J. clin. Microbiol., 26: 990-994, 1988.
5. FORGHANI, B. & SCHMIDT, N.J. - Antigen requirements, sensitivity and specificity of enzyme immunoassays for measles and rubella viral antibodies. J. clin. Microbiol., 9: 657-664, 1979.
6. MAHONY, J.B.; CASTRICIANO, S. & CHERNESKY, M.A. - Cost and performance analysis of haemagglutination-inhibition, passive haemagglutination, radial haemolysis, and enzyme immunoassay for measuring rubella antibody. J. virol. Meth., 18: 133-142, 1987.
7. PANNUTI, C.S.; MORAES, J.C.; SOUZA, V.A.U.F. et al. - Measles antibody prevalence after mass immunization in Săo Paulo, Brazil. Bull. Wld. Hlth. Org., 69: 557-560, 1991.
8. WEISSFELD, A.S.; GEHLE, W.D. & SONNENWIRTH, A.C. - Comparison of several test systems used for determination of rubella immune status. J. clin. Microbiol., 16: 82-85, 1982.

Correspondence to:

Vanda Akico Ueda Fick de Souza

Instituto de Medicina Tropical de São Paulo

Av. Dr. Enéas de Carvalho Aguiar 470

05403-000 São Paulo, SP, Brazil

Fax (011) 852.3622

Publication Dates

Publication in this collection
21 Sept 2006
Date of issue
Aug 1995

History

Accepted
06 Apr 1995
Received
09 Jan 1995

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ASSAD, F. & LJUNGARS-ESTEVES, K. - Rubella. World impact. Rev. infect. Dis, 7 (suppl.):S29-S36, 1985.

[2] 2. BEST, J.M. & O'SHEA, S. - Togaviridae: rubella. In: LENNETTE, E.H.; HALONEN, P. & MURPHY, F.A., ed. Laboratory diagnosis of infectious diseases. Principles and practice. New York, Springer-Verlag, 1988. v.2, p.435-450.

[3] 3. FERRARO, M.J.; KALLAS, W.M.; WELCH, K.P. & LAU, A.Y. - Comparison of a new, rapid enzyme immunoassay with a latex agglutination test for qualitative detection of rubella antibodies. J. clin. Microbiol., 25: 1722-1724, 1987.

[4] 4. FIELD, P.R.; HO, D.W.T. & CUNNINGHAM, A.L. - Evaluation of rubella immune status by three commercial enzyme-linked immunosorbent assays. J. clin. Microbiol., 26: 990-994, 1988.

[5] 5. FORGHANI, B. & SCHMIDT, N.J. - Antigen requirements, sensitivity and specificity of enzyme immunoassays for measles and rubella viral antibodies. J. clin. Microbiol., 9: 657-664, 1979.

[6] 6. MAHONY, J.B.; CASTRICIANO, S. & CHERNESKY, M.A. - Cost and performance analysis of haemagglutination-inhibition, passive haemagglutination, radial haemolysis, and enzyme immunoassay for measuring rubella antibody. J. virol. Meth., 18: 133-142, 1987.

[7] 7. PANNUTI, C.S.; MORAES, J.C.; SOUZA, V.A.U.F. et al. - Measles antibody prevalence after mass immunization in Săo Paulo, Brazil. Bull. Wld. Hlth. Org., 69: 557-560, 1991.

[8] 8. WEISSFELD, A.S.; GEHLE, W.D. & SONNENWIRTH, A.C. - Comparison of several test systems used for determination of rubella immune status. J. clin. Microbiol., 16: 82-85, 1982.