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Leishmania (Viannia) panamensis - induced cutaneous leishmaniasis in susceptible and resistant mouse strains

Leishmaniose cutânea induzida pela Leishmania (Viannia) panamensis em linhagens de camundongos suscetíveis e resistentes

Abstracts

We studied the susceptibility to Leishmania (Viannia) panamensis in strains of mice. The C57BL/6 strain was resistant and showed self-controlled lesion at the injected foot pad. The BALB/c and DBA/2J strains were susceptible and showed a foot swelling that started day 20 post-infection and progressed to a tumour-like lesion in later period of observation. The CBA/HJ strain was found to be of intermediary resistance. In contrast to other known cutaneous leishmaniasis in mice, the lesion in L. (V.) panamensis-infected mice was restricted to the inoculation site in the skin. In addition, we studied the development of cellular response and antibodies against Leishmania antigen in BALB/c and C57BL/6 strains. The proliferative response of lymph node cells against L. (V.) panamensis antigen was biphasic in both strains. An initial response was seen on day 20, followed by a refractory period between 40 and 80 days and a second response around fourth month post-infection. The response in the latter period was higher in C57BL/6 strain than in BALB/c strain. BALB/c strain presented much higher anti-Leishmania antibody level than C57BL/6 strain. The model and the correlation of immunological variables and the course of the infection are discussed.

Cutaneous leishmaniasis; Leishmania (Viannia) panamensis; Experimental model; Mice; Immunology


Estudamos a susceptibilidade à Leishmania (Viannia) panamensis em linhagens de camundongos BALB/c, DBA/2J, CBA/HJ e C57BL/6. Os camundongos da linhagem C57BL/6 eram resistentes, apresentando lesões autolimitantes na pata. Os das linhagens BALB/c e DBA/2J eram suscetíveis, apresentando edema na pata, evidente aos 20 dias pós-infecção que progrediu para uma lesão tipo tumoral nas fases tardias. Os animais da linhagem CBA/HJ apresentaram resistência intermediária. Em contraste a outros modelos de leishmaniose cutânea murina, a lesão nos camundongos infectados pela L. (V.) panamensis mostrou ser restrita ao local de inoculação na pele. Estudamos também o desenvolvimento de resposta celular e anticorpos anti-Leishmania nas linhagens BALB/c c C57BL/6. A resposta proliferativa de células do linfonodo a antígenos de L. (V.) panamensis foi bifásica em ambas as linhagens. Uma resposta inicial foi observada com 20 dias de infecção, seguida por uma fase refratária entre 40 e 80 dias e uma segunda resposta ao redor do quarto mês de infecção. A resposta nesta segunda fase estava mais alta na linhagem C57BL/6 do que na BALB/c. Por outro lado, os camundongos BALB/c apresentaram níveis de anticorpo anti-Leishmania muito mais elevados que os da linhagem C57BL/6. O modelo e a correlação das variáveis imunológicas com o curso da infecção são discutidos.


IMMUNOLOGY

Leishmania (Viannia) panamensis - induced cutaneous leishmaniasis in susceptible and resistant mouse strains

Leishmaniose cutânea induzida pela Leishmania (Viannia) panamensis em linhagens de camundongos suscetíveis e resistentes

H. GotoI,II; J. I. RojasIII; L. SporrongIV; P. de CarreiraV; C. SánchezIII; A. ÖrnIV

ILaboratory of Infectious Diseases' Pathology, Department of Pathology and Department of Preventive Medicine, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil

IIDepartment of Immunology, Karolinska Institute, Sweden

IIIDepartamento de Farmacologia, Facultad de Medicina, Universidad de Panamá, Panamá, República de Panamá

IVMicrobiology and Tumourbiology Centre, Karolinska Institute, Stockholm, Sweden

VDepartamento de Parasitologia, Facultad de Medicina, Universidad de Panamá, Panamá, República de Panamá

Correspondence to Correspondence to: Dra. Hiro Goto Faculdade de Medicina da USP, Departamento de Patologia, Laboratório de Patologia de Moléstias Infecciosas Av. Dr. Arnaldo, 455 01246-903, São Paulo, S.P., Brazil

SUMMARY

We studied the susceptibility to Leishmania (Viannia) panamensis in strains of mice. The C57BL/6 strain was resistant and showed self-controlled lesion at the injected foot pad. The BALB/c and DBA/2J strains were susceptible and showed a foot swelling that started day 20 post-infection and progressed to a tumour-like lesion in later period of observation. The CBA/HJ strain was found to be of intermediary resistance. In contrast to other known cutaneous leishmaniasis in mice, the lesion in L. (V.) panamensis-infected mice was restricted to the inoculation site in the skin. In addition, we studied the development of cellular response and antibodies against Leishmania antigen in BALB/c and C57BL/6 strains. The proliferative response of lymph node cells against L. (V.) panamensis antigen was biphasic in both strains. An initial response was seen on day 20, followed by a refractory period between 40 and 80 days and a second response around fourth month post-infection. The response in the latter period was higher in C57BL/6 strain than in BALB/c strain. BALB/c strain presented much higher anti-Leishmania antibody level than C57BL/6 strain. The model and the correlation of immunological variables and the course of the infection are discussed.

Keywords: Cutaneous leishmaniasis; Leishmania (Viannia) panamensis; Experimental model; Mice; Immunology.

RESUMO

Estudamos a susceptibilidade à Leishmania (Viannia) panamensis em linhagens de camundongos BALB/c, DBA/2J, CBA/HJ e C57BL/6. Os camundongos da linhagem C57BL/6 eram resistentes, apresentando lesões autolimitantes na pata. Os das linhagens BALB/c e DBA/2J eram suscetíveis, apresentando edema na pata, evidente aos 20 dias pós-infecção que progrediu para uma lesão tipo tumoral nas fases tardias. Os animais da linhagem CBA/HJ apresentaram resistência intermediária. Em contraste a outros modelos de leishmaniose cutânea murina, a lesão nos camundongos infectados pela L. (V.) panamensis mostrou ser restrita ao local de inoculação na pele. Estudamos também o desenvolvimento de resposta celular e anticorpos anti-Leishmania nas linhagens BALB/c c C57BL/6. A resposta proliferativa de células do linfonodo a antígenos de L. (V.) panamensis foi bifásica em ambas as linhagens. Uma resposta inicial foi observada com 20 dias de infecção, seguida por uma fase refratária entre 40 e 80 dias e uma segunda resposta ao redor do quarto mês de infecção. A resposta nesta segunda fase estava mais alta na linhagem C57BL/6 do que na BALB/c. Por outro lado, os camundongos BALB/c apresentaram níveis de anticorpo anti-Leishmania muito mais elevados que os da linhagem C57BL/6. O modelo e a correlação das variáveis imunológicas com o curso da infecção são discutidos.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENT

We acknowledge the Karolinska International Research Training program and SAREC for supporting this project, CADESCA for helping the KIRT II program in Central America, the Gorgas Memorial Laboratory for technical support and Magnus Gidlund for critical comments. Hiro Goto was supported by scholarship from Universidade de São Paulo - Banco Interamericano de Desenvolvimento program.

Recebido para publicação em 15/05/1995.

Aceito para publicação em 03/10/1995.

Carried out at Department of Immunology, Karolinska Institute, Stockholm, Sweden.

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  • Correspondence to:

    Dra. Hiro Goto
    Faculdade de Medicina da USP, Departamento de Patologia, Laboratório de Patologia de Moléstias Infecciosas
    Av. Dr. Arnaldo, 455
    01246-903, São Paulo, S.P., Brazil
  • Publication Dates

    • Publication in this collection
      22 Sept 2006
    • Date of issue
      Dec 1995

    History

    • Accepted
      03 Oct 1995
    • Received
      15 May 1995
    Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000 - São Paulo - SP - Brazil, Tel. +55 11 3061-7005 - São Paulo - SP - Brazil
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