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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.38 no.2 São Paulo Mar./Apr. 1996 



Histopathology and immunocytochemical study of type 3 and type 4 complement receptors in the liver and spleen of dogs naturally and experimentally infected with Leishmania (Leishmania) chagasi


Histopatologia e estudo imunocitoquímico dos receptores do tipo 3 e 4 do complemento no fígado e baço de cães natural e experimentalmente infectados com Leishmaniose Visceral



Wagner Luiz Tafuri; Washington Luiz Tafuri; Alfredo José Afonso Barbosa; Marilene Susan Marques Michalick; Odair Genaro; João Carlos França-Silva; Wilson Mayrink; Evaldo Nascimento

Department of General and Anatomical Pathology, UFMG

Correspondence to




The objective of this study was to compare the histopathological changes and expression of CR3 and CR4 in the liver and spleen of dogs naturally and experimentally infected with L. chagasi. The basic histopathological lesions observed mainly in naturally infected dogs were: epithelioid hepatic granulomas, hyperplasia and hypertrophy of Kupffer cells, Malpigui follicles and mononucleated cells of the red pulp of the spleen. Sections from the liver and spleen by immunocytochemistry technique showed the presence of CD11b,c\CD 18 antigens in the control and infected animals and no qualitative or quantitative differences in the liver. Nevertheless, CD18 was always increased in the spleen of naturally and experimentally infected dogs. These results indicate that there is a difference in the activaton of CD 18 in both experimental and natural cases of canine visceral leishmaniasis that should play an important role in the immunological response to Leishmania chagasi infection.

Keywords: Dogs; Leishmania chagasi; Histopathology; Immunocytology; Complement receptors.


Os objetivos deste trabalho visaram uma análise comparativa das alterações histopatológicas e da expressão de CR3 e CR4 no fígado e baço de cães natural e experimentalmente infectados com L. chagasi. As lesões histopatológicas fundamentais observadas principalmente nos cães naturalmente infectados foram: os granulomas epitelióides hepáticos, a hiperplasia e a hipertrofia das células de Kupffer, dos folículos de Malpighi e das células mononucleadas da polpa vermelha do baço. Os cortes de fígado e baço corados pela técnica de imunocitoquímica mostraram a presença dos antígenos CD11b,c\CD18 nos animais controles e infectados, sem diferenças qualitativas e quantitativas no fígado. Entretanto, no baço dos cães natural e experimentalmente infectados a expressão de CD 18 (subunidade b2 da molécula comum aos leucócitos) foi sempre aumentada. Em leishmaniose a resposta imune celular é considerada a mais importante na resolução da doença. A expressão de CD11 b,c\CD 18 pelos macrófagos deve representar um papel central na resposta celular. Estes resultados indicam que tanto na leishmaniose visceral canina experimental ou natural existe uma diferença na ativação de CD 18, o qual deve exercer uma importante função na resposta imunológica na infecção por Leishmania chagasi.



Full text available only in PDF format.

Texto completo disponível apenas em PDF.




The authors wish to thank Professors Ivan Barbosa Machado Sampaio, Department of Zootechny, Veterinary School, UFMG, for assistance with the statistical analyses, and Professor Peter F. Moore, School of Veterinary Medicine, University of California - Los Angeles and Davis, USA, for kindly making available canine monoclonal antibodies. Financial support: FINEP, CNPq, PRPq-UFMG, PADCT/ CNPq/Biotecnologia.



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Correspondence to:
Wagner L. Tafuri
Department of General Pathology, ICB/UFMG, Campus da Pampulha
Av. Antônio Carlos 6627
30270-901 Belo Horizonte, Minas Gerais, Brasil

Recebido para publicação em 27/09/95
Aceito para publicação em 21/03/96

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