A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp

Un método simple y económico para generar la atmósfera microaerofílica para el aislamiento de Campylobacter sp

THECNICAL TRIAL

A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp

Un método simple y económico para generar la atmósfera microaerofílica para el aislamiento de Campylobacter sp

Francisco Hernández

Faculty of Microbiology, University of Costa Rica, San José, Costa Rica

SUMMARY

Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.

Keywords:Campylobacter: technical assay; Microaerobiosis, poultry.

RESUMEN

Se inocularon por duplicado 138 muestras de heces de pollos en agar de Blaser. Un juego de las placas se incubó en jarras con sobres generadores de microaerobiosis (CampyPak). Las otras placas se incubaron en bolsas plásticas tipo "Zip-lock" (7 x 8 pulgadas) y la atmósfera microaerofílica se generó inflando la bolsa luego de retener la respiración por 20 seg, desinflándola e inflándola nuevamente (4 veces) y finalmente sellándola. Se aisló Campylobacter de 127 (96.2%) de las muestras incubadas en la jarra y de 129 (98%) de los especimenes incubados en la bolsa. La metodologia propuesta ofrece una posibilidad económica para los investigadores concientes de los costos de operación.

Full text available only in PDF format.

Texto completo disponible sólo en PDF.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENTS

This work was supported by the Research Vicepresident of the University of Costa Rica. I thanks Dr. Danilo Garcia for his edition and valuable suggestions concerning the manuscript.

Recebido para publicação em 11/06/1996

Aceito para publicação em 08/07/1996

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Publication Dates

• Publication in this collection
  22 Sept 2006
• Date of issue
  June 1996

History

• Accepted
  08 July 1996
• Received
  11 June 1996