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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.38 no.4 São Paulo July/Aug. 1996

http://dx.doi.org/10.1590/S0036-46651996000400006 

MICOLOGY

 

New records of Histoplasma capsulatum from wild animals in the Brazilian Amazon

 

Novos registros de Histoplasma capsulatum em animais silvestres na Amazônia brasileira

 

 

Roberto Daibes NaiffI; Toby Vincent BarrettI; Maricleide de Farias NaiffI; Luiz Carlos de Lima FerreiraII; Jorge Ramon Arias1

IInstituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil
IIUniversidade do Amazonas, Departamento de Patologia Tropical e Medicina Legal-FCS, Manaus, AM, Brasil

Correspondence to

 

 


SUMMARY

Twenty-eight isolates of Histoplasma capsulation were obtained from eight species of forest mammals from the States of Amazonas, Pará and Rondônia in the Amazon Region of Brazil. Primary isolates were obtained by inoculating triturated liver and spleen tissue intradermally and intraperito-neally in hamsters. Mycological diagnosis in hamsters presenting lesions was confirmed by histopathology and culture on Sabouraud dextrose-agar. Infected hamsters developed signs of disease within two to nine months; all had disseminated visceral lesions and most also had skin lesions at the sites of inoculation. None of the hamsters inoculated with skin macerates of the original hosts developed histoplasmosis, and histopathological examination of the viscera of the wild hosts failed to reveal H. capsulation. Prevalence of infection was considerably higher in females than in males both for the opossum Didelphis marsupialis and for total wild animals (479) examined. It is proposed that canopy-dwelling mammals may acquire the infection from conidia borne on convective currents in hollow trees with openings at ground-level.

Keywords: Enzootic histoplasmosis; Sex-related prevalence; Amazon Basin.


RESUMO

Vinte e oito amostras de Histoplasma capsulatum foram obtidas de oito espécies de mamíferos silvestres nos Estados do Amazonas, Pará e Rondônia. Os isolamentos foram feitos mediante inoculação de amostras trituradas de fígado e baço em hamsters por via intradérmica e intraperitoneal. O diagnóstico micológico nos hamsters que apresentaram lesões foi confirmado por histopatologia e cultivo em meio dextrose-agar de Sabouraud. Os hamsters infectados desenvolveram sinais de doença após dois a nove meses; todos apresentaram lesões disseminadas nas vísceras e a maioria apresentou também lesões cutâneas nos locais da inoculação. Nenhum dos hamsters inoculados com material de pele dos hospedeiros originais desenvolveu histoplasmose, e H. capsulatum não foi detectado nos exames histopatológicos dos animais silvestres. A prevalência de infecção foi consideravelmente mais alta entre fêmeas, tanto para Didelphis marsupialis como para o total de animais silvestres examinados (479). Propõe-se que as infecções detectadas em animais arborícolas podem ser explicadas pelo transporte de esporos do fungo em correntezas convectivas dentro de árvores ocas que tenham uma abertura ao nível do solo e outra a nível proximo das copas.


 

 

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 

 

ACKNOWLEDGEMENTS

This work was financed in part by UNDP/ WORLD BANK/ WHO-TRD Special Programme for Research and Training in Tropical Diseases and the bilateral cooperation agreements ELETRONORTE/INPA, POLONOROESTE/INPA and FUNDAÇÃO BANCO DO BRASIL/INPA. We thank Osvaldo Guriam, Vice-Secretary of Health of Ji-Parana (Rondônia); Raimundo das Graças M. Lobato, Director of the Hospital of Tropical Medicine in Porto Velho; and Geraldo F.L.R. Coutinho, Director of the DNER highways department at Marabá (Pará) for logistic support; and Lourival M. Castro, Francisco L. Santos, Plínio E. Silva and Paulo E. Albuquerque for technical assistance. Figure 4 is by Artêmio Coelho da Silva.

 

REFERENCES

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Correspondence to:
Roberto Naiff
Coordenação de Pesquisas em Ciências da Saúde, Institute) Nacional de Pesquisas da Amazonia
Caixa Postal 478
69011-970 Manaus, AM, Brazil

Recebido para publicação em 30/04/1996
Aceito para publicação em 12/07/1996

 

 

1 Current address: Organização Pan-Americana da Saúde, S.E.N. Lote 19. 70800-400 Brasilia, DF, Brasil.

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