FUNGEMIA CAUSED BY Candida SPECIES IN A CHILDREN'S PUBLIC HOSPITAL IN THE CITY OF SÃO PAULO, BRAZIL: STUDY IN THE PERIOD 2007-2010

Fungemia por espécies de Candida em Hospital Pediátrico da cidade de São Paulo, Brasil: estudo no período de 2007 a 2010

Abstracts

Candidemia remains a major cause of morbidity and mortality in the health care environment. The epidemiology of Candida infection is changing, mainly in relation to the number of episodes caused by species C. non-albicans. The overall objective of this study was to evaluate the frequency of yeasts of the genus Candida, in a four-year period, isolated from blood of pediatric patients hospitalized in a public hospital of the city of São Paulo, Brazil. In this period, yeasts from blood of 104 patients were isolated and, the identified species of Candida by phenotypic and genotypic methods were: C. albicans (39/104), C. tropicalis (25/104), C. parapsilosis (23/104), Pichia anomala (6/104), C. guilliermondii (5/104), C. krusei (3/104), C. glabrata (2/104) and C. pararugosa (1/104). During the period of the study, a higher frequency of isolates of C. non-albicans (63.55%) (p = 0.0286) was verified. In this study we verified the increase of the non-albicans species throughout the years (mainly in 2009 and 2010). Thus, considering the peculiarities presented by Candida species, a correct identification of species is recommended to lead to a faster diagnosis and an efficient treatment.

Candida ; Candidemia; Pediatric

Candidemia permance como a maior causa de morbidade e mortalidade em ambiente hospitalar. A epidemiologia de infecções por Candida vem se alterando, principalmente em relação ao número de episódios causados por espécies não-albicans. Este estudo teve como objetivo avaliar a frequência, em um período de quatro anos, de leveduras do gênero Candida isoladas de sangue de pacientes pediátricos internados em hospital público da cidade de São Paulo, Brasil. Neste período foram isoladas leveduras de sangue de 104 pacientes, e as espécies de Candida identificadas, por métodos fenotípicos e genotípicos, foram: C. albicans (39/104), C. tropicalis (25/104), C. parapsilosis (23/104), Pichia anomala (6/104), C. guilliermondii (5/104), C. krusei (3/104), C. glabrata (2/104) e C. pararugosa (1/104). Em todo período do estudo foi observada maior frequência de isolamento de C. não-albicans (63,55%) (p = 0,0286). Neste estudo verificou-se aumento das espécies não-albicans ao longo dos anos (principalmente em 2009 e 2010), assim, ressalta-se que correta identificação em nível de espécie é recomendável, para que isso acarrete diagnóstico rápido e tratamento eficaz.

INTRODUCTION

Candidemia is an important concern in the clinical medicine related to the public health²⁰, mainly because of the high mortality rates in children and adults, 30% and 50% respectively³⁸. The risk factors for the acquisition of this infection are similar in both ages and include a long-term hospitalization in an Intensive Care Unit, previous bacterial infection, central venous catheter use, parenteral nutrition and immunosuppression. The yeasts of the genus Candida represent the third/fourth pathogens responsible for the bloodstream infections¹⁰,¹¹,²⁴ and their epidemiology has been well studied in the United States and Europe, but not in Latin America. In Brazil, the incidence rates are fragmented when considering data from all regions of the country¹⁰,²⁷.

Although C. albicans remains the most frequent species of yeast isolated from bloodstream infections, over the last years the number of candidemias caused by non-albicans species has been increasing. Being that, some studies have reported that from 40% to 50% of the infections are caused by other species of Candida¹⁶,³¹,³³. Moreover, invasive infections caused by non-albicans species are more difficult to be treated due to its eventual innate or acquired resistance to antifungal agents. Therefore the treatment administration should be based on the species-level identification²³.

Many epidemiological studies have described the distribution of the species, antifungal susceptibility and risk factors in the adult population¹⁰,³³. However, there are few studies about candidemia in the pediatric population³⁰,³⁴. Some studies, done in hospitals from different geographic regions, report that the most frequent species that cause bloodstream infections in pediatric patients are C. albicans and C. parapsilosis¹⁶,²⁸. In adults, the most frequent isolated species are C. albicans, C. glabrata, C. parapsilosis, C. tropicalis and C. krusei²⁸.

In Brazil, the most commonly isolated species in pediatric patients are C. albicans, C. parapsilosis and C. tropicalis. In adult patients the most common species are the same as in pediatric patients, except because of the species C. tropicalis that is the second most frequent species in adults¹⁰. This high isolation of C. parapsilosis in pediatric patients could be explained by the fact that this species is related to prematurity, presence of central venous catheters and the use of total parenteral nutrition³⁰.

Anatomical and physiological differences between the pediatric and adult patients change the susceptibility to infections caused by different species of Candida, which therefore influences the antifungal treatment approach, including issues related to the toxicity of the drugs, pharmacokinetic and dosage⁴¹.

Considering the existence of a restricted amount of data to guide the clinical decisions to child patients with invasive fungal infections when compared to adults with similar infections, this study had as its objective to evaluate the frequency of the yeast species isolated from blood during four years in a pediatric hospital in São Paulo, Brazil.

PATIENTS

From 2007 to 2010, yeasts isolated from blood of 104 patients, represented by children from five to 11 years old, were studied. Being that, each isolated represented a different episode of candidemia. The patients were hospitalized in a tertiary infant hospital with 90 hospital beds located in the city of São Paulo, Brazil.

MATERIAL AND METHODS

Isolate Process: The blood was seeded in a biphasic culture medium BACTEC (Difco^tm, USA). Once the fungal growth was detected, the medium was seeded in Sabouraud dextrose agar added to chloramphenicol to the yeasts isolation, after that it was seeded in a CHROmagar^® Candida Chromogenic Medium to ensure the purity of the isolates and then phenotypically identified.

Phenotypic identification: The conventional identification of all the isolates was performed according to the protocol of KURTZMAN et al.²⁰, using the germ tube test, microculture, auxanogram and zymogram. The phenotypic identification between C. albicans and C. dubliniensis was performed evaluating the colony color in a CHROmagar^® medium, the thermotolerance³⁴ and the growth in a hypertonic medium³.

Genotypic identification: The molecular technique was used to the complex C. parapsilosis and for the differentiation of C. albicans and C. dubliniensis.

DNA extraction: The DNA extraction from the culture was carried out using the PrepMan™ Ultra Sample Preparation reagent Quick Reference Card kit (Applied Biosystems, USA), according to the commercial protocol.

Differentiation of the complex C. parapsilosis (C. parapsilosis sensu stricto, C. orthopsilosis, C. metapsilosis): To the differentiation of the complex C. parapsilosis (C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis) a Ribosomal DNA ITS sequencing was performed³⁷. The amplification of the ITS-rDNA region was accomplished using the universal primers V9G / LS266¹³ and the Big Dye terminator 3.1 kit (Applied Biosystems, USA). The edition of the nucleotide sequences was performed and analyzed by comparing them with the sequences available in the GenBank using the BLASTn (http://blast.ncbi.nlm.nih.gov/Blast.cgi).

Differentiation between C. albicans and C. dubliniensis: It was carried out according to MANNARELLI & KURTZMAN²². The PCR was performed with two pairs of specific primers: one pair for C. dubliniensis (sense CDU2 and anti-sense NL4CAL) and another pair for C. albicans (sense CAL5 and anti-sense: NL4CAL). The presence or not of amplified fragments was visualized by using the agarose gel electrophoresis.

Quality control: To control the quality of the phenotypic and genotypic methods, the strain patterns used were: ATCC 64548 (C. albicans), ATCC 777 (C. dubliniensis), C. parapsilosis (ATCC 22019), C. orthopsilosis (ATCC 96141) and C. metapsilosis (ATCC 96143).

Statistical analysis: Quantitative variables were analyzed by the unpaired Student's t test or Mann-Whitney (Prism version 5.0, GraphPad Software Inc., La Jolla, CA, USA). A p value < 0.05 was considered to be statistically significant.

RESULTS

During the period of the study, a total of 104 yeast strains were isolated from hemocultures. Figure 1 represents the total of yeasts isolated from blood per year.

Fig. 1 -
Number of strains and species isolated from blood of 104 pediatric patients, hospitalized in a children's public hospital, from 2007 to 2010.

The isolation of C. albicans in the children's public hospital studied was kept in the first two years, with the frequency of 42.3% in 2007 and 48.3% in 2008. In 2009 we had a decrease in the isolation rate (21.7%) followed by an increase in 2010 (36%). The isolation of C. non-albicans has always been higher than the one verified for C. albicans (p = 0.0286) and differently from these species, we had a considerable increase in the isolation rate for non-albicans species in the last two periods. The change in the numbers of isolates of C. albicans and non-albicans from 2007 to 2010 can be observed in the Figure 2.

Fig. 2 -
Frequency of isolates of C. albicans and non-abicans per year in a children's public hospital.

In the four years of this study, the total frequency of identified species of Candida were: 37.5% of C. albicans (39/104), 24.03% of C. tropicalis (25/104), 22.11% of C. parapsilosis (23/104), 5.8% of Pichia anomala (6/104), 4.8% of C. guilliermondii (5/104), 2.88% of C. krusei (3/104), 1.92% of C. glabrata (2/104) and 0.96% of C. pararugosa (1/104). In the genotypic testing for differentiation molecular of C. albicans and C. dubliniensis and C. parapsilosis complex, the result was consistent with C. albicans and C. parapsilosis in 100% of the samples. The frequency of the species per year can be observed in Figure 1.

DISCUSSION

Candida albicans is an etiologic agent in about 40-60% of the bloodstream infections in pediatric patients¹⁴,²⁸. Studies have reported an increase in the rate of infections caused by non-albicans species⁶. In research, done in a pediatric hospital in the USA which had 97 patients studied, species of Candida (being the most frequent C. albicans and C. parapsilosis) were the most commonly isolated among fungemic patients, present in 91% of the cases. The species of Candida more commonly isolated from blood have changed along the time. In general, C. albicans represents 60% of the cases of isolates from blood between 1991 and 1996 and 48% of the isolates between 1997 and 2001, what was in accordance in the groups of ages¹. We emphasize in our study that 104 children with fungemia were observed in a shorter period than the study previously mentioned.

In another study performed in adults and pediatric patients with candidemia in a hospital in Porto Rico, the non-albicans species represented around 83% of the isolates; meanwhile C. albicans was responsible for 17% of the cases¹². In our study, C. non-albicans (62.5%) were also more isolated than C. albicans (37.5%). We noticed that in 2007 C. albicans represented 42.3% of the isolates, in 2008 we had a small increase of isolates of C. albicans (48.3%), but in 2009 and 2010 we had a decrease of isolates of these species, 21.7% and 36% respectively. The increase of the isolation rates of non-albicans species in patients with candidemia in Brazilian hospitals has been reported for a while⁹,³¹. Different authors have also reported an increase in the incidence of candidemia caused by non-albicans species, represented by C. parapsilosis, C. glabrata, C. krusei and C. tropicalis. These authors affirmed that, however in the last years C. albicans has been responsible for more than a half of the cases of candidemias, the rates of infections caused by non-albicans species have slowly increased¹⁸,²⁵,⁴¹. In epidemiology, this could be associated with severe immunosuppression or illness, prematurity, exposure to broad-spectrum antibiotics, older patients and selective pressure exerted by prophylactic antifungals in patients at high risk of developing invasive fungal infections³⁵.

Recent studies have reported that C. tropicalis is one of the species that most frequently causes urinary tract infections and candidemia, especially in patients with cancer²⁶.

Recent studies evinced that the increase of C. parapsilosis incidence may be explained by some relevant factors such as parenteral nutrition and the use of central venous catheters, and this is explained by the fact that this species has the facility to form biofilm²,¹⁹. However, possible sources of infections and routes of transmission of infections caused by C. parapsilosis are not always easily identified and in some cases the epidemiology of the infections remains undercover⁵. Therefore, the epidemiological relevance of this study should be highlighted considering that C. parapsilosis was one of the most frequently isolated species and had our special attention.

Comparing nowadays with the 1980's, we can notice that, at present, there is a higher frequency of species albicans causing bloodstream infections as relevant pathogens like C. glabrata in the USA. Meanwhile, in Europe, Canada and Latin America, C. parapsilosis and C. tropicalis are the most frequent species responsible for causing bloodstream infections²⁸.

The increase of fungemias caused by non-albicans species like C. glabrata and C. krusei, is really relevant to the therapy. C. krusei has an inherent resistance to fluconazole and C. glabrata is relatively resistant to fluconazole, but fluconazole is the most frequently chosen antifungal drug to be used in this cases.

C. guilliermondii has also been isolated in our study. However, infections caused by C. guilliermondii are not common. They occurred in patients with cancer, hematological neoplasias as nosocomial infections by the use of central venous catheter having a high mortality rate⁴⁰.

Blood, skin, nails, tissues and, less frequent urine and genital tract, represent the most common places where the fungi have been more frequently isolated as a possible pathogen³⁶.

Regarding the P. anomala strains, it is highlighted in a previous study that it had already been isolated from a child patient in a pediatric hospital³⁰. PASQUALOTTO et al.³², in another study carried out in a pediatric hospital in Brazil, from October 2002 to December 2004, an outbreak of P. anomala had been detected. Seventeen patients had developed fungemia due to this species and molecular studies demonstrated that this outbreak was caused by just one strain. This yeast was not isolated, neither from the health professionals' hands nor from the environment.

In India, a hospital outbreak caused by P. anomala in a 23-month period (from April 1996 to February 1998), that had the spread from the fungi on the health professionals' hands, led to the commission of the hospital infection control to have a strict and educational control to improve the hand washing. Analyzing the potential risk factors to infections by P. anomala in children, we verified that some of these factors are: prematurity, very low-weight, a long-term hospitalization⁸, the use of central venous catheters, total parenteral nutrition, lipid emulsion, and the previous use of antimicrobial and other invasive procedures⁴.

Based on the finding of this study and the literature, we could notice the relevance of studying nosocomial infections cases by yeasts, especially from the genus Candida. We verified the increase of the non-albicans species throughout the years (mainly in 2009 and 2010). The non-albicans species cases have been increasing year after year in these nosocomial infection cases and concerning all these data, it is highlighted a correct species-level identification to lead to a fast diagnosis and efficient treatment.

ACKNOWLEDGEMENTS

The authors express their thanks to PhD Marina Korte for his revision of the English text, to CNPQ and FAPESP for their financial support and to Dr. Arnaldo Lopes Colombo for support in the molecular study.

REFERENCES

• ¹
  Abelson JA, Moore T, Bruckner D, Deville J, Nielsen K. Frequency of fungemia in hospitalized pediatric inpatients over 11 years at a tertiary care institution. Pediatrics. 2005;116:61-7.
• ²
  Almirante B, Rodríguez D, Cuenca-Estrella M, Almela M, Sanchez F, Ayats J, et al. Epidemiology, risk factors, and prognosis of Candida parapsilosis bloodstream infections: case-control population-based surveillance study of patients in Barcelona, Spain, from 2002 to 2003. J Clin Microbiol. 2006;44:1681-5.
• ³
  Alves SH, Milan EP, de Laet Sant'ana P, Oliveira LO, Santurio JM, Colombo AL. Hypertonic Sabouraud broth as a simple and powerful test for Candida dubliniensis screening. Diagn Microbiol Infect Dis. 2002;43:85-6.
• ⁴
  Aragão PA, Oshiro ICV, Manrique EI, Gomes CC, Matsuo LL, Leone C, et al. Pichia anomala outbreak in a nursery: exogenous source? Pediatr Infect Dis J. 2001;20:843-8.
• ⁵
  Asbeck ECV, Huang YC, Markham AN, Clemons NV, Stevens DA. Candida parapsilosis fungemia in neonates: genotyping results suggest healthcare workers hands as source, and review of published studies. Mycopathologia. 2007;164:287-93.
• ⁶
  Awasthi AK, Jain A, Awasthi S, Ambast A, Singh K, Mishra V. Epidemiology and microbiology of nosocomial pediatric candidemia at a northern Indian tertiary care hospital. Mycopathologia. 2011;172:269-77.
• ⁷
  Cantón E, Pemán J, Quindós G, Eraso E, Miranda-Zapico I, Álvarez M, et al. Prospective multicenter study of the epidemiology, molecular identification, and antifungal susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis isolated from patients with candidemia. Antimicrob Agents Chemother. 2011;55:5590-6.
• ⁸
  Chakrabarti A, Singh K, Narang A, Singhi S, Batra R, Rao KLN, et al. Outbreak of Pichia anomala infection in the pediatric service of a tertiary-care center in Northern India. J Clin Microbiol. 2001;39:1702-6.
• ⁹
  Colombo AL, Nucci M, Salomão R, Branchini ML, Richtmann R, Derossi A, et al. High rate of non-albicans candidemia in Brazilian tertiary care hospitals. Diagn Microbiol Infect Dis. 1999;34:281-6.
• ¹⁰
  Colombo AL, Nucci M, Park BJ, Nouér SA, Arthington-Skaggs B, Matta DA, et al. Epidemiology of candidemia in Brazil: a nationwide sentinel surveillance of candidemia in eleven medical centers. J Clin Microbiol. 2006;44:2816-23.
• ¹¹
  Colombo AL, Guimarães T, Silva LR, de Almeida Monfardini LP, Cunha AK, Rady P, et al. Prospective observational study of candidemia in São Paulo, Brazil: incidence rate, epidemiology, and predictors of mortality. Infect Control Hosp Epidemiol. 2007;28:570-6.
• ¹²
  Conde-Rosa A, Amador R, Perez-Torres D, Colón E, Sánchez-Rivera C, Nieves-Plaza M, et al. Candidemia distribution, associated risk factors, and attributed mortality at a university-based medical center. ProHealth Sci. 2010;29:26-9.
• ¹³
  De Hoog GS, Gerrits van den Ende AHG. Molecular diagnostics of clinical strains of filamentous basidiomycetes. Mycoses. 1998;41:183-9.
• ¹⁴
  Festekjian A, Neely M. Incidence and predictors of invasive candidiasis associated with candidemia in children. Mycoses. 2011;54:146-53.
• ¹⁵
  Garcia-Effron G, Canton E, Pemán J, Dilger A, Romá E, Perlin DS. Epidemiology and echinocandin susceptibility of Candida parapsilosis sensu lato species isolated from bloodstream infections at a Spanish university hospital. J Antimicrob Chemother. 2012;67:2739-48.
• ¹⁶
  García-Rodríguez J, Cantón E, Pemán J, Alvarez M, Ezpeleta G, Gómez-Nieto A, et al. Incidencia etaria y geográfica y patrón de sensibilidad a los antifúngicos de las especies de Candida causantes de candidemia en la población pediatrica española. Enferm Infecc Microbiol Clin. 2013;31:363-8.
• ¹⁷
  Gonçalves SS, Amorim CS, Nucci M, Padovan ACB, Briones MRS, Melo ASA, et al. Prevalence rates and antifungal susceptibility profiles of the C. parapsilosis species complex, results from a nationwide surveillance of candidaemia in Brazil. Clin Microbiol Infect. 2010;16:885-7.
• ¹⁸
  Hinrichsen SL, Falcão E, Vilella TAS, Rêgo L, Lira C, Almeida L, et al. Candida isolates in tertiary hospitals in northeastern Brazil. Braz J Microbiol. 2009;40:325-8.
• ¹⁹
  Horasan ES, Ersöz G, Göksu M, Otag F, Kurt AO, Karaçorlu S, et al. Increase in Candida parapsilosis fungemia in critical care units: a 6-years study. Mycopathologia. 2010;170:263-8.
• ²⁰
  Kurtzman CP, Fell JW, Boekhout T. The yeasts, a taxonomic study. Burlington: Elsevier Science; 2011.
• ²¹
  Lortholary O, Desnos-Ollivier M, Sitbon K, Fontanet A, Bretagne S, Dromer, F, et al. Recent exposure to caspofungin or fluconazole influences the epidemiology of candidemia: a prospective multicenter study involving 2,441 patients. Antimicrob Agents Chemother. 2011;55:532-8.
• ²²
  Mannarelli BM, Kurtzman CP. Rapid identification of Candida albicans and other human pathogenic yeasts by using short oligonucleotides in a PCR. J Clin Microbiol. 1998;36:1634-41.
• ²³
  Morace G, Borghi E, Iatta R, Amato G, Andreoni S, Brigante G, et al. Antifungal susceptibility of invasive yeast isolates in Italy: the GISIA3 study in critically ill patients. BMC Infect Dis. 2011;11:130.
• ²⁴
  Motta AL, Almeida GMD, Almeida Jr JN, Burattini MN, Rossi F. Candidemia epidemiology and susceptibility profile in the largest Brazilian teaching hospital complex. Braz J Infect Dis. 2010;14:441-8.
• ²⁵
  Muñoz P, Giannella M, Fanciulli C, Guinea J, Valerio M, Rojas L, et al. Candida tropicalis fungemia: incidence, risk factors, and mortality in a general hospital. Clin Microbiol Infect. 2011;17:1538-45.
• ²⁶
  Negri M, Martins M, Henriques M, Svidzinski TIE, Azeredo J, Oliveira R. Examination of potential virulence factors of Candida tropicalis clinical isolates from hospitalized patients. Mycopathologia. 2009;169:175-82.
• ²⁷
  Nucci M, Queiroz-Telles F, Tobón AM, Restrepo A, Colombo AL. Epidemiology of opportunistic fungal infections in Latin America. Clin Infect Dis. 2010;51:561-70.
• ²⁸
  Nucci M, Queiroz-Telles F, Alvarado-Matute T, Tiraboschi IN, Corte J, Zurita J, et al. Epidemiology of candidemia in Latin America: a laboratory-based survey. Plos One. 2013;8:e59373.
• ²⁹
  Oeser C, Lamagni T, Heath PT, Sharland M, Ladhani S. The epidemiology of neonatal and pediatric candidemia in England and Wales, 2000-2009. Pediatr Infect Dis J. 2013;32:23-6.
• ³⁰
  Paula CR, Krebs VLJ, Auler ME, Ruiz LS, Matsumoto FE, Silva EH, et al. Nosocomial infection in newborns by Pichia anomala in a Brazilian intensive care unit. Med Mycol. 2006;44:479-84.
• ³¹
  Paula CR, Montelli AC, Ruiz LS, Batista GCM, Matsumoto FE, Volperarnoni M, et al. Infecção hospitalar fúngica: experiência em hospitais públicos de São Paulo. Prática Hosp. 2007;52:63-6.
• ³²
  Pasqualotto AC, Sukiennik TC, Severo LC, De Amorim CS, Colombo AL. An outbreak of Pichia anomala fungemia in a Brazilian pediatric intensive care unit. Infect Control Hosp Epidemiol. 2005;26:553-8.
• ³³
  Ruiz LS, Sugizaki MF, Montelli AC, Matsumoto FE, Pires MFC, Da Silva BCM, et al. Fungemia by yeasts in Brazil: occurrence and phenotypic study of strains isolated at the public hospital, Botucatu, São Paulo. J Mycol Med. 2005;15:13-21.
• ³⁴
  Ruiz LS, Khouri S, Hahn RC, da Silva EG, de Oliveira VK, Gandra RF, et al. Candidemia by species of the Candida parapsilosis complex in children's hospital: prevalence, biofilm production and antifungal susceptibility. Mycopathologia. 2013;175:231-9.
• ³⁵
  Sardi JCO, Scorzoni L, Bernardi T, Fusco-Almeida AM, Mendes Giannini MJS. Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol. 2013;62:10-24.
• ³⁶
  Savini V, Catavitello C, Di Marzio I, Masciarelli G, Astolfi D, Balbinot A, et al. Pan-azole-resistant Candida guilliermondii from a leukemia patient's silent funguria. Mycopathologia. 2010;169:457-9.
• ³⁷
  Sullivan DJ, Westerneng TJ, Haynes KA, Bennet DE, Coleman DC. Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals. Microbiology. 1995;141(Pt7):1507-21.
• ³⁸
  Tavanti A, Davidson AD, Gow NAR, Maiden MCJ, Odds FC. Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. J Clin Microbiol. 2005;43:284-92.
• ³⁹
  Viudes A, Pemán J, Cantón E, Úbeda P, López-Ribot JL, Gobernado M. Candidemia at a tertiary-care hospital: epidemiology, treatment, clinical outcome and risk factors for death. Eur J Clin Microbiol Infect Dis. 2002;21:767-74.
• ⁴⁰
  Yamamura M, Makimura K, Fujisaki R, Satoh K, Kawakami S, Ota HNY. Polymerase chain reaction assay for specific identification of Candida guilliermondii (Pichia guilliermondii). J Infect Chemother. 2009;15:214-8.
• ⁴¹
  Yapar N, Pullukcu H, Avkan-Oguz V, Sayin-Kutlu S, Ertugrul B, Sacar S, et al. Evaluation of species distribution and risk factors of candidemia: a multicenter case-control study. Med Mycol. 2011;49:26-31.
• ⁴²
  Zaoutis TE, Prasad PA, Localio AR, Coffin SE, Bell LM, Walsh TJ, et al. Risk factors and predictors for candidemia in pediatric intensive care unit patients: implications for prevention. Clin Infect Dis. 2010;51:38-45.

Publication Dates

• Publication in this collection
  Jul-Aug 2014

History

• Received
  3 July 2013
• Accepted
  10 Jan 2014