PREVALENCE OF Entamoeba histolytica/Entamoeba dispar IN THE CITY OF CAMPINA GRANDE, IN NORTHEASTERN BRAZIL

Prevalência de Entamoeba histolytica/Entamoeba dispar na cidade de Campina Grande, Nordeste do Brasil

Abstracts

There is a clear need to perform epidemiological studies to find the true prevalence of Entamoeba histolytica around the world. The evaluation of this prevalence has been hindered by the existence of two different species which are morphologically identical, but genetically different, namely E. histolytica, which causes amebiasis, and E. dispar, which is non-pathogenic. In Brazil, the E. dispar has been detected in communities in the Southeastern (SE) and Northeastern (NE) regions with poor sanitation. However, individuals infected with E. histolytica have been identified in other regions. There is an absence of reports on the prevalence of these parasites in the state of Paraíba, which also has areas with poor sanitary conditions where a high prevalence of the E. histolytica/E. dispar complex has been detected in children from urban slums. The present study evaluated the prevalence of E. histolytica and E. dispar in 1,195 asymptomatic children between two and 10 years of age, living in a sprawling urban slum in Campina Grande, in the state of Paraíba, in Northeastern Brazil. These children were examined and their feces samples were analyzed microscopically. A total of 553 children tested positive for the E. histolytica/E. dispar complex, and 456 of the positive samples were tested with the E. histolytica II® ELISA kit. All 456 samples were negative for the presence of the adhesin E. histolytica specific antigen. The evidence suggests that in this community E. histolytica is absent and E. dispar is the dominant species.

Entamoeba histolytica ; Entamoeba dispar ; Prevalence; Adhesin detection immunoassay

A prevalência mundial de Entamoeba histolytica não está bem estabelecida. Este fato deve-se à complicação derivada da existência de duas espécies morfologicamente idênticas, mas geneticamente diferentes: a E. histolytica que causa amebíases e a E. dispar descrita como não patogênica. No Brasil, em comunidades com precárias condições sanitárias e endêmicas para várias parasitoses, localizadas nas regiões Sudeste (SE) e Nordeste (NE), somente E. dispar tem sido encontrada, porém outras regiões, apresentam indivíduos infectados por E. histolytica. Na região agreste do Estado da Paraíba (NE) que apresenta as mesmas precárias condições sanitárias, não tem sido reportada prevalência específica destes parasitos, embora fosse encontrada alta prevalência do complexo E. dispar/E. histolytica em crianças em favela urbana. O presente estudo foi realizado em favela da cidade de Campina Grande, Estado da Paraíba, onde 1.195 crianças de dois a 10 anos sem sintomatologia foram examinadas. Amostras de fezes destas crianças foram analisadas microscopicamente, encontrando-se 553 positivas para o complexo E. dispar/E. histolytica. Do total de amostras positivas, 456 foram submetidas à pesquisa do antígeno especifico para E. histolytica pelo teste ELISA E. histolytica II®,obtendose resultado negativo para a presença do antígeno adesina específico de E. histolytica, em todas as amostras testadas. Os resultados sugerem que nesta comunidade não há infecção por E. histolytica, e que E. dispar é a espécie dominante na região.

INTRODUCTION

Until 1993, the studies on the prevalence of amoebiasis showed that approximately 10% of the world's population was infected with Entamoeba histolytica. However, only 1% of these infections developed into an invasive form of the disease, with a mortality factor of about 100,000 cases per year²⁶,²⁷. Entamoeba histolytica was redescribed and separated into two species: Entamoeba dispar, non-pathogenic to humans; and Entamoeba histolytica, pathogenic and the cause of invasive intestinal and extra intestinal disease⁸,¹⁶. These species are morphologically identical, but new methods for parasite detection based on enzymes, lectin antigens, or DNA sequencing have been extremely helpful in determining the most current data¹,¹¹,¹⁸,²¹,²⁷. These studies show that E. dispar infection is more frequent than E. histolytica infection in most of the endemic regions²,¹²,¹³,¹⁴,¹⁵,²⁰. An accurate worldwide distribution of these species has not yet been determined. It is important to obtain complete epidemiological profiles, particularly in case of pediatric patients, to avoid unnecessary antiamoebic pharmacotherapy. The Pan American Health Organization does not recommend treatment to asymptomatic individuals presenting cysts of E. histolytica/E. dispar in stools without the specific identification of E. histolytica²⁶.

The prevalence of E. histolytica infection is variable among different geographic regions in Brazil due to differences in sanitation and socioeconomic conditions. For example, the city of Belém, in the state of Pará, in Northern Brazil, presents a high frequency of hepatic amoebiasis cases (29.35%)¹⁹,²². In neighboring regions, such as in the city of Manaus, Amazonas State, the E. histolytica prevalence is 6.8%, whereas in the Northeast city of Fortaleza, Ceará, the prevalence is 14.9%³,⁵. On the other hand, in the city of Maceió, Alagoas, and in several cities, in Pernambuco (in Northeastern Brazil), several parasites are endemic due to poor sanitation. However, no autochthonous cases of E. histolytica are present in spite of the great prevalence of non-pathogenic amoebas⁹,¹⁰,¹⁷. Campina Grande in the state of Paraíba is located in the same Northeastern region of Brazil and displays the same sanitary and socioeconomic conditions; however, the distribution of Entamoeba species is unknown, particularly in children. The current study was designed to determine the prevalence of E. histolytica in an urban slum of this city.

METHODS

The population chosen for this study was the community of Pedregal, in the city of Campina Grande. The E. histolytica/E. dispar complex was detected through microscopic examinations in a previous survey²³ of this community (in 89.9% of the children). Campina Grande is a city located at 7°13′50″S and 35°52′52″W, at 552 meters above sea level, with temperatures varying between 15 °C and 30 °C, and a relative humidity of 82%. The city's average pluvial index is approximately 810 mm/year²⁴. It has a population of around 400,000 inhabitants. Pedregal has 9,267 inhabitants, of which 16.62% are under the age of 10. This community lacks proper waste disposal, sewage, and drainage systems. Most of the sewage is discharged in a central channel that overflows during the rainy season, flooding major access routes to school and homes used by the whole community. The study was conducted from January to November 2007, when researchers and a Community Health Worker visited children between two and 10 years of age in their homes. The children were clinically examined for amoebiasis symptoms: dysentery, diarrhea, cramping abdominal pain, rectal tenesmus, vomiting and hepatomegaly¹⁸.

The samples of feces were recovered in containers supplied during the visit. Every child that brought their stools became part of the study. The study was approved by the Ethics Committee in Human Research of the Federal University of Campina Grande on May 22^nd, 2006, and authorized by the Secretary of Municipal Health in Campina Grande (protocol # 20060314-001, 05/30/2006). Detailed explanations of the protocols to be used in the study were provided to the parents or guardians who voluntarily signed the form of consent. The 1,195 fecal samples collected from children between two and 10 years of age were separated into two aliquots: one aliquot was submitted to the Ritchie Formalin-Ether concentration method, and the pellets examined for the presence of erythrocytes, E. histolytica/E. dispar complex cysts and trophozoites, with 0.9% saline and Lugol's iodine solutions. The second aliquot was stored without preservatives at -20 °C for the ELISA Entamoeba differentiation test.

The E. histolytica II® (TechLab, USA) ELISA kit was used for the detection of E. histolytica specific adhesin antigen in 456 stool samples. This test was performed on thawed samples randomly selected from the population that had been identified as positive for the E. histolytica/E. dispar complex by microscopic examination; the test was performed strictly according to the manufacturer's instructions.

RESULTS

Light microscope examination: Analysis of 1,195 stool samples demonstrated that 314 (26.3%) were free of any kind of intestinal parasite and 553 (46.3%) were positive for the presence of four-nuclei amoeba. Giardia lamblia (229 samples, 19.2%) and Entamoeba coli (369, 30.9%) were the next most prevalent protozoa observed in the stool samples. Ascaris lumbricoides (314, 26.3%) and Trichuris trichiura (198, 16.6%) were the most prevalent helminths; Ancylostoma sp (6, 0.5%), Strongyloides stercoralis (5, 0.4%), Hymenolepis sp (12, 1%), and Taenia sp (4, 0.3%) were also observed in the samples. Associations of parasites were observed among 492 (88.9%) children infected with the E. histolytica/E. dispar complex. Forty-five children showed unformed feces. Six of them showed the presence of numerous bacteria, moderate leucocytes and mucus in the feces. No erythrocytes were observed. The remainder 39 unformed feces were concomitantly parasitized by the E. histolytica/E. dispar complex and by G. lamblia and/or helminths, mostly Ascaris lumbricoides. Infected children were pharmacologically treated according to the parasite infection detected. The symptoms evaluated - diarrhea, abdominal pain, vomiting and anorexia - were common to all children, but nothing was identified as gastrointestinal amoebic infection. No rare symptoms, such as mucous sanguineous evacuation, rectal tenesmus, cramping abdominal pain or hepatomegaly were observed.

Entamoeba differentiation test: 456 (82.5%) positive stool samples (showing the presence of E. histolytica/E. dispar complex cysts through microscopy) were submitted to the E. histolytica differentiation test. None of these samples were reactive to the antibody against the specific E. histolytica adhesin antigen.

DISCUSSION

Considering the sanitary conditions of endemic regions, the Brazilian Ministry of Health recommends the treatment of every child resulting positive for amoebas to avoid spreading of this parasite⁶. The accurate identification of the amoeba species found in stools and the true determination of E. histolytica prevalence in endemic regions become necessary, once studies have shown the neurotoxicity of metronidazole - the first line antibiotic drug to treat amoeba infections⁴.

In the previous study conducted in 2005²³, a prevalence of 89.9% for the E. histolytica/E. dispar complex was determined in children living in this community, through microscopic examination of stool samples. These children were treated with metronidazole²³. In this study, analyses of the feces of 1,195 children under the age of 10 were performed, revealing that 46.3% of the population remains infected. All of them were asymptomatic for amoebiasis as observed through clinical examination. The presence of parasites in 73.7% of the population under study, demonstrates that the poor hygienic conditions, important risk factor of fecal-oral transmission, were not improved in the community. The socio-economic and environmental conditions were the same. In this context, the observed decrease in E. histolytica/E. dispar complex prevalence is probably due to treatments conducted previously with metronidazole.

Only one stool sample was collected once the community declined to provide more samples, even after extensive explanations on the benefits of the examination of multiple samples for confirmatory diagnosis. Ritchie's concentration method has showed limitations, associated with the preservation of trophozoites. However, this technique allowed us to observe four nuclei-amoeba cysts. The microscopic examination was performed by two experienced parasitologists to minimize the reported low sensitivity of the methodology used⁷.

No specific E. histolytica adhesin antigens were detected in the 456 cyst positive stool samples for the E. histolytica/E. dispar complex. Based on the high specificity and sensitivity of the ELISA test used¹¹, it could be assumed that in this population, the high prevalence of Entamoeba cysts (46.3% of the samples studied) does not represent cases of E. histolytica infection. Although precise identification of the E. dispar species requires the use of molecular methods, this evidence suggests that in this community E. dispar is the dominant species. The absence of E. histolytica in the universe under study is in accordance with reports from Pernambuco⁹, Maceió in Alagoas¹⁰, and São Leopoldo in Rio Grande do Sul²⁵, using the same ELISA method for E. histolytica adhesin; and with results from Salvador in Bahia²¹, and the state of Pernambuco¹⁷ using PCR. The analyzed sample (1,195 children) represents 77.57% of the child population in this urban slum (1,540 children); therefore, the results obtained are representative for this community. Most of the reports for the prevalence of E. histolytica were obtained from cities situated in coastal regions. The city of Campina Grande is in the “agreste” region, inland and in between humid Atlantic Forest and the semiarid region of the Northeast. Nevertheless, the sanitary conditions and risk factors²³,²⁴ in this city are similar to other regions such as Fortaleza⁵, Manaus³, and Belém²², displaying a high prevalence of E. histolytica. There are no records of the prevalence of E. histolytica in the states of Paraíba, Rio Grande do Norte and Maranhão. Hence, it is highly recommended that a national epidemiological survey be performed to determine with accuracy the prevalence of E. histolytica in the various regions of the country, and assist in reviewing the adopted strategies for medicating asymptomatic cyst carriers.

REFERENCES

• ¹
  Aca IS, Kobayashi S, Carvalho Júnior LB, Tateno S, Takeushi T. Prevalence and pathogenicity of Entamoeba histolytica in three different regions of Pernambuco, Northeast Brazil. Rev Inst Med Trop Sao Paulo. 1994;36:519-24.
• ²
  Beltramino JC, Sosa H, Gamba N, Busquets N, Navarro L, Virgolini S. et al. Sobrediagnóstico de amebiasis en niños con disentería. Arch Argent Pediatr. 2009;107:510-4.
• ³
  Benetton ML, Gonçalves AV, Meneghini ME, Silva EF, Carneiro M. Risk factors for infection by the Entamoeba histolytica/E.dispar complex: an epidemiological study conducted in outpatient clinics in the city of Manaus, Amazon region, Brazil. Trans R Soc Trop Med Hyg. 2005;99:532-40.
• ⁴
  Bottenberg MM, Hegge KA, Eastman DK, Kumar R. Metronidazole-induced encephalopathy: a case report and review of the literature. J Clin Pharmacol. 2011;51:112-6.
• ⁵
  Braga LL, Gomes ML, Silva MW, Paiva C, Sales A, Mann BJ. Entamoeba histolytica and Entamoeba dispar infections as detected by monoclonal antibody in an urban slum in Fortaleza, Northeastern Brazil. Rev Soc Bras Med Trop. 2001;34:467-71.
• ⁶
  Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Amebíase doenças infecciosas e parasitárias: guia de bolso. 3a ed. Brasília: Ministério da Saúde; 2004. vol. I. (Série textos básicos de saúde).
• ⁷
  Chacín-Bonilla L. Diagnóstico microscópico de la amibiasis: método obsoleto pero necesario en el mundo en desarrollo. Invest Clin. 2011;52:291-4.
• ⁸
  Diamond LS, Clark CG. A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. J Eukaryot Microbiol. 1993;40:340-4.
• ⁹
  Dourado A, Maciel A, Aca IS. Ocorrência de Entamoeba histolytica/Entamoeba dispar em pacientes ambulatoriais de Recife, PE. Rev Soc Bras Med Trop. 2006;39:388-99.
• ¹⁰
  Duarte IAC. Prevalência da Entamoeba histolytica em alunos de escolas públicas da cidade de Maceió. [tese]. Recife: Universidade Federal de Pernambuco, Medicina Tropical; 2006.
• ¹¹
  Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J. Laboratory diagnostic techniques for Entamoeba species. Clin Microbiol Rev. 2007;20:511-32.
• ¹²
  Guzmán C, López MC, Reyes P, Gómez J, Corredor A, Agudelo CA. Diferenciación de Entamoeba histolytica y Entamoeba dispar en muestras de materia fecal por detección de adhesina de E. histolytica mediante ELISA. Biomédica. 2001;21:167-71.
• ¹³
  Haque R, Ali IM, Petri WA Jr. Prevalence and immune response to Entamoeba histolytica infection in preschool children in Bangladesh. Am J Trop Med Hyg. 1999;60:1031-4.
• ¹⁴
  Leiva B, Lebbad M, Winiecka-Krusnell J, Altamirano I, Tellez A, Linder E. Overdiagnosis of Entamoeba histolytica and Entamoeba dispar in Nicaragua: a microscopic, triage parasite panel and PCR study. Arch Med Res. 2006;37:529-34.
• ¹⁵
  Nesbitt R, Mosha FW, Katki HA, Ashraf M, Assenga C, Lee CM. Amebiasis and comparison of microscopy to ELISA technique in detection of Entamoeba histolytica and Entamoeba dispar. J Natl Med Assoc. 2004;96:671-7.
• ¹⁶
  Petithory JC, Brumpt LC, Poujade F. Entamoeba histolytica (Schaudinn 1903) and Entamoeba dispar (E. Brumpt 1925) are 2 different species. Bull Soc Pathol Exot. 1994;87:231-7.
• ¹⁷
  Pinheiro SMB, Carneiro RM, Aca IS, Irmão JI, Morais MA Jr, Coimbra MRM, et al. Determination of the prevalence of Entamoeba histolytica and E. dispar in the Pernambuco State of northeastern Brazil by a polymerase chain reaction. Am J Trop Med Hyg. 2004;70:221-4.
• ¹⁸
  Redondo RB, Méndez LM, Baer G. Entamoeba histolytica and Entamoeba dispar. Differentiation by enzyme-linked immunosorbent assay (ELISA) and its clinical correlation in pediatric patients. Parasitol Latinoam. 2006;61:37-42.
• ¹⁹
  Salles JM, Moraes LA, Salles MC. Hepatic amebiasis. Braz J Infect Dis. 2003;7:96-110.
• ²⁰
  Samie A, Obi LC, Bessong PO, Stroup S, Houpt E, Guerrant RL. Prevalence and species distribution of E. histolytica and E. dispar in the Venda region, Limpopo, South Africa. Am J Trop Med Hyg. 2006;75:565-71.
• ²¹
  Santos FLN, Gonçalves MS, Soares NM. Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil. Braz J Infect Dis. 2011;15:119-25.
• ²²
  Silva MC, Monteiro PCS, Araujo VB, Silva VJ, Povoa MM. Determinação da infecção por Entamoeba histolytica em residentes da área metropolitana de Belém, Pará, Brasil, utilizando ensaio imunoenzimático (ELISA) para detecção de antígenos. Cad Saúde Pública. 2005;21:969-73.
• ²³
  Silva MTN, Pontes A, Aragão P, Andrade J, Tavares-Neto J. Prevalência de parasitas intestinais em crianças com baixos indicadores socioeconômicos em Campina Grande (Paraíba). Rev Bahiana Saúde Pública. 2005;29:121-5.
• ²⁴
  Silva MTN, Souza MV, Bragagnoli G, Pereira TGR, Malagueño E. Atopic dermatitis and ascariasis in children aged 2 to 10 years. J. Pediatr (Rio J). 2010;86:53-8.
• ²⁵
  Tomé JBS, Tavares RG. Diferenciação entre Entamoeba histolytica e Entamoeba dispar por meio de ensaio imunoenzimático para pesquisa de antígenos em amostras fecais. Rev Inst. Adolfo Lutz. 2007;66:305-7.
• ²⁶
  WHO/PAHO/UNESCO report. A consultation with experts on amoebiasis. Mexico City, Mexico 28-29 January. Epidemiol Bull. 1997;18(1):13-4.
• ²⁷
  Ximénez C, Morán P, Rojas L, Valadez A, Gomez A. Reassessment of the epidemiology of amebiasis: state of the art. Infect Genet Evol. 2009;9:1023-32.

Publication Dates

• Publication in this collection
  Sep-Oct 2014

History

• Received
  7 Jan 2012
• Accepted
  19 Mar 2014