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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.61  São Paulo  2019  Epub Feb 14, 2019

http://dx.doi.org/10.1590/s1678-9946201961007 

ORIGINAL ARTICLE

Increased serum gastrin in patients with different clinical forms of Chagas disease coinfected with Helicobacter pylori

Jacqueline Batista Sousa1 

Renata Margarida Etchebehere2 

Dulciene Maria de Magalhães Queiroz3 

Fernanda Machado Fonseca1 

Bianca Bontempi Batista1 

Iracema Saldanha Junqueira4 

Sílvia Maria Perrone Camilo5 

Adriana Gonçalves de Oliveira1 
http://orcid.org/0000-0003-4561-5503

1Universidade Federal do Triângulo Mineiro, Instituto de Ciências Biológicas e Naturais, Uberaba, Minas Gerais, Brazil

2Universidade Federal do Triângulo Mineiro, Serviço de Patologia Cirúrgica, Uberaba, Minas Gerais, Brazil

3Universidade Federal de Minas Gerais, Faculdade de Medicina, Laboratório de Pesquisa em Bacteriologia, Belo Horizonte, Minas Gerais, Brazil

4Universidade Federal do Triângulo Mineiro, Serviço de Endoscopia, Uberaba, Minas Gerais, Brazil

5Universidade Federal do Triângulo Mineiro, Serviço de Gastroenterologia, Uberaba, Minas Gerais, Brazil

ABSTRACT

Trypanosoma cruzi and Helicobacter pylori (HP) are pathogens that cause chronic diseases and have been associated with hypergastrinemia. The aim of this study was to evaluate the fasting gastrin levels in patients with different clinical forms of Chagas disease (CD), coinfected or not by HP. The enrolled individuals were outpatients attending at the university hospital. HP infection was assessed by serology and 13 C-urea breath test. Fasting serum gastrin concentration was measured by chemiluminescence assay. Gastric endoscopic and histological features were also evaluated. Associations between CD and serum gastrin level were evaluated in a logistical model, adjusting for age, gender and HP status. A total of 113 patients were evaluated (45 with Chagas disease and 68 controls). In the multivariate analysis, increasing serum gastrin levels (OR= 1.02; 95% CI= 1.01-1.12), increasing age (OR= 1.05; 95% CI= 1.02 - 1.09) and HP-positive status (OR = 2.88; 95% CI = 1.10 - 7.51) remained independently associated with CD. The serum gastrin levels were significantly higher in the group of patients with the cardiodigestive form ( P = 0.03) as well as with digestive form ( P = <0.001) of Chagas disease than in the controls. In conclusion, patients with cardiodigestive and digestive clinical forms of CD have increased basal serum gastrin levels in comparison with controls. Moreover, we also demonstrated that H. pylori coinfection contributes to the hypergastrinemia shown in CD.

Key words: Helicobacter pylori; Chagas disease; Gastrin; Chronic gastritis; Acid secretion; Gastric physiology

INTRODUCTION

Chagas disease, caused by the intracellular protozoan Trypanosoma cruzi ( T. cruzi ), is endemic in Latin American countries where it is primarily transmitted to humans by contact with faeces of triatomine vectors 1 . In the past decades, the migration of populations from endemic areas has contributed to the spread of Chagas disease to the USA, Canada, many European and some Western Pacific countries 2 .

The acute phase of Chagas disease is usually asymptomatic, although a high number of parasites circulate in the bloodstream of infected individuals. Then, the disease progresses to an asymptomatic chronic phase called the indeterminate form, which is prolonged and a few or no parasites are found in blood. Commonly, around 20% to 30% of infected patients will develop irreversible cardiovascular and/or gastrointestinal lesions with damage on enteric nervous system. These alterations characterize the symptomatic forms of chronic Chagas disease, i.e., cardiac, digestive or cardiodigestive form 1,3 .

An abnormally high fasting serum gastrin level associated with hyposecretion of gastric acid has been reported in chagasic patients with the digestive form 4-8 . Gastrin, a hormone produced in G cells mainly located in the gastric antral mucosa, is a potent secretor of gastric acid. Histamine and acetylcholine, released from enterochromaffin-like cells and from enteric neurons, respectively, also stimulate the acid secretion while somatostatin, secreted by oxyntic and antral D cells, is the major inhibitor of acid secretion 9 . Indeed, the regulation of gastric acid secretion in parietal cells is achieved by a highly coordinated interaction among neural, hormonal and paracrine pathways.

Gastrin may be increased in other various clinical conditions such as the gastric infection with Helicobacter pylori ( H. pylori ) 10 . This Gram-negative bacterium is recognized as the main cause of chronic gastritis throughout the world and develops an important role in peptic ulcer, gastric carcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma 11 . H. pylori (HP) causes diverse effects on gastric acid secretion depending mainly on the location within the stomach and the degree of inflammation. Usually, antral predominant gastritis results in hypersecretion of acid and can lead to duodenal ulceration. The predominant gastritis on corpus or pangastritis results in atrophic gastritis and abnormally low secretion of gastric acid associated with marked hypergastrinemia. These alterations can strongly favor the development of gastric adenocarcinoma 10,12-14 .

Studies showing an increased basal serum gastrin levels in patients with Chagas disease evaluated only the digestive form of the disease and most of them were conducted before the discovery of H. pylori , which has been shown to be highly prevalent in chagasic patients. Thus, the aim of this study was to evaluate whether fasting hypergastrinemia also occurs in patients with other clinical forms of Chagas disease, coinfected or not with H. pylori .

MATERIAL AND METHODS

This cross-sectional study was approved by the Ethics Committee of the Federal University of Triangulo Mineiro and was conducted in accordance with the Declaration of Helsinki. All volunteers signed the informed consent form and answered a questionnaire to obtain the social-demographic data. Clinical information was obtained by reviewing the patients’ medical records.

Subjects and clinical samples

A total of 113 outpatients (45 with Chagas disease and 68 without Chagas Disease (CD) – controls) attended at the university hospital from April 2012 to January 2015 were evaluated. The exclusion criteria were previous therapy for H. pylori eradication, history of peptic ulcer, gastrointestinal cancer, renal failure or concomitant severe illness. Individuals taking proton pump inhibitors, H 2 blockers and H 2 -antihistamines or those who underwent upper gastrointestinal tract surgery were also excluded. A blood sample was collected from each patient under fasting conditions for the gastrin measurements, serological diagnosis of Chagas disease and H. pylori infection. Additionally, all patients were submitted to 13 C-urea breath test ( 13 C-UBT) for H. pylori diagnostic.

Diagnosis of Chagas disease

Enzyme-linked immunosorbent assay (ELISA; Chagatest ELISA-WIENER, Rosario, Argentina), passive hemagglutination (Chagatest HAI-WIENER, Rosario, Argentina) and indirect immunofluorescence tests (Imuno-Con Chagas-WAMA, Sao Carlos, Brazil) were performed according to the manufacturer’s instructions. Chagasic patients showed positive reactions to at least two serological tests and control patients were negative by the three tests. Chagasic patients were classified according to the clinical form of disease (i.e., indeterminate, digestive, cardiac or cardiodigestive) based on clinical examination, electrocardiography and radiological exams of the chest, esophagus and colon.

H. pylori status

H. pylori infection was assessed by serology and 13 C-UBT. Patients with discordant results were excluded. Patients were considered H. pylori -positive (HP-positive) if both tests were positive and H. pylori -negative (HP-negative) if both tests were negative. IgG antibodies specific to H. pylori were detected using a commercial ELISA kit (Serion Immundiagnostica GmbH, Würzburg, Germany), according to the manufacturer’s instructions and results were expressed as positive or negative. The 13 C-UBT was performed after an overnight fast. After collection of a basal breath sample, an oral dose of 13 C-urea on a citric acid base was administered. An additional breath sample was collected after 30 min. Breath samples were analyzed using an isotope-selective non-dispersive infra-red spectrometer (NDIRIS; Wagner Analytical Systems, Bremen, Germany). A change >4.0‰ in delta over baseline (DOB) was considered HP-positive.

Measurements of serum gastrin levels

Gastrin-17 concentration was measured by a chemiluminescence assay (IMMULITE® 2000 immunoassay system; Siemens Medical Solutions Diagnostics, Los Angeles, EUA) and the values were expressed in pg/mL. All gastrin assays were performed in duplicate. The normal value for fasting gastrin-17 using this assay is <115 pg/mL.

Histological analysis

Endoscopic biopsy specimens were obtained from the antrum and corpus of the stomach of patients who underwent EGD by medical indication. Biopsy samples were fixed overnight in buffered formalin embedded in paraffin, sliced to 4 µm thickness, and stained with haematoxylin and eosin for histological examination. The Warthin-Starry staining was used for detection of spiral H. pylori . The degree of gastric mucosal inflammation and activity were also assessed. The location of gastritis was classified according to the presence of mononuclear cells in the antrum, corpus or in both. Glandular atrophy and intestinal metaplasia were classified as absent or present.

Statistical analysis

The association of Chagas disease with the basal serum gastrin level (dependent variable) including H. pylori status, age and gender (independent variables) was tested by a univariate analysis. All variables with P values ≤ 0.25 were included in the full model of the logistic regression. Odds ratios (OR) and 95% confidence intervals (CI) were used as an estimate of the risk. The Hosmer-Lemeshow goodness-of-fit test was used to evaluate the fitness of the model 15 . In addition to visual examination of histograms and box plots, the Kolmogorov-Smirnov (K-S) test or Shapiro-Wilk (SW) test, based on the number of each subgroups, was used for assessing the normality of data. Because data did not show a normal distribution even after log transformation, a two-tailed Mann-Whitney U-test (median / range) was used to detect the difference of serum gastrin levels among groups. Otherwise, because the age of participants was normally distributed, the groups were compared by a two-tailed Student’s T test (mean / standard deviation). Data were analyzed with the statistical package for the social sciences (SPSS) version 23.0 (SPSS Inc., Chicago, IL, USA). For comparison of groups with dichotomous variables the two-tailed chi-squared test (with Yates’ correction) or the Fisher´s exact test was used. P value ≤0.05 was considered significant.

RESULTS

Demographic data

Among the 113 included subjects, 45 were chagasic patients (14 men and 31 women; mean age 64.3 ± 10.5 years) and 68 were controls without CD (29 male and 39 female; mean age 60.4 ± 10.1 years). When the groups were compared, no difference was observed regarding the gender ( P = 0.29; OR = 1.65; 95% CI = 0.74 - 3.64), but the disease group participants were older than the control ones ( P = 0.05). Most chagasic (35 - 77.8%) patients and controls (43 - 63.2%) were HP-positive.

Median gastrin levels

Median serum gastrin levels were higher in chagasic patients than in controls, both in HP-positive patients (65.10 pg/mL; range, 11-557 pg/mL vs. 39.80 pg/mL; range, 12-172 pg/mL, respectively; P = 0.01) and HP-negative ones (49.15 pg/mL; range, 16-670 pg/mL vs. 23.0 pg/mL; range, 10-133 pg/mL, respectively; P = 0.009), but higher in the HP-positive groups than in the negative ones.

In order to identify variables independently associated with Chagas disease, data were analyzed in a logistic regression model. In the univariate analysis, the basal serum gastrin level, HP status and age, but not the gender, were selected. In the multivariate analysis, the increasing of serum gastrin levels, the increasing age and HP-positive status remained independently associated with Chagas disease (Table 1).

Table 1 - Variables associated with Chagas disease (n=45) in comparison with controls (n=68) in a multivariate analysis. 

Variables Univariate Multivariate


P value OR 95% CI P value
Gender 0.29
Increasing age 0.05 1.05 1.02-1.09 0.04
H. pylori + status 0.09 2.88 1.10-7.51 0.03
Increasing gastrin level 0.008 1.02 1.01-1.12 0.03

The Hosmer-Lemeshow test showed good fitness of the logistic regression model ( P = 0.25; 8 degrees of freedom; 10 steps). + positive; OR- odds ratio; 95% CI- 95% confidence interval.

Among chagasic patients, 10 (22.2%) had the cardiodigestive form, 12 (26.7%) the digestive form, 12 (26.7%) the indeterminate form, and 11 (24.4%) the cardiac form of the disease. Figure 1 shows the basal serum gastrin levels of controls and of patients with different clinical forms of Chagas disease. Serum gastrin levels were significantly higher in the group of patients with the cardiodigestive form as well as with the digestive form of disease than in the control group.

Figure 1 - Box plots representing the basal serum gastrin levels among the control and chagasic patients with different clinical forms of the Chagas disease. The horizontal bar across the box indicates the median and the capped bars indicate the minimum and maximum data values. The upper and lower limits of the boxes represent the 75 th and 25 th percentiles, respectively. No difference was observed between the controls and the indeterminate ( P = 0.53) and cardiac ( P = 0.13) forms of the disease. Also, the cardiodigestive clinical form did not differ from the digestive form (P = 0.65). The data were analyzed by the two-tailed Mann-Whitney U test. 

Nevertheless, no difference was observed between controls and patients with either the indeterminate ( P = 0.53) or the cardiac ( P = 0.13) forms of disease. Also, in the group of chagasic patients, significant higher levels of serum gastrin were observed in the digestive than in the cardiac ( P = 0.008) and in the indeterminate ( P = 0.01) forms of the disease. In addition, the cardiodigestive form of the disease did not differ from the digestive one (P = 0.65). No significant difference was observed in respect to the prevalence of HP and age among different subgroups of clinical forms of Chagas disease ( P > 0.05).

In the subgroup of patients who underwent EGD (n = 35), serum gastrin levels were significantly higher ( P = 0.004) in the nine patients with the digestive clinical form of Chagas disease (median, 72.20 pg/mL; range, 36-246 pg/mL) than in the 26 controls (median, 32.20 pg/mL; range, 11 - 133 pg/mL). Histological exams detected the presence of the bacterium in both, the antral and the oxyntic gastric mucosa in seven (77.8%) chagasic patients and in 13 (50.0%) controls ( P = 0.29; OR = 3.50; 95% CI = 0.61 – 20.13). Gastritis was observed in the gastric mucosa of all HP-positive patients, without differences in the number of either mononuclear or polymorphonuclear cells between chagasic and control patients ( P = 1.0 for both, two tailed Fisher exact test). Chagasic patients (n= 4; 44.4%) did not differ from controls (n= 5; 19.2%) in respected to the presence of intestinal metaplasia in the stomach mucosa ( P = 0.58; OR = 2.17; 95%CI = 0.45 - 10.49). Gastric or duodenal peptic ulcer was not observed in chagasic patients.

DISCUSSION

The findings of the present study showed that basal serum gastrin levels are higher in patients with Chagas disease than in non-chagasic controls. This result persisted even after adjustment for potential confounding factors such as age, gender and HP infection. It should be noted that, for the first time, it was demonstrated that hypergastrinemia is present in the cardiodigestive clinical form of Chagas disease as well as in digestive form.

It is known that rising intraluminal pH of the stomach is the main factor stimulating gastrin production and secretion. However, the abnormal high gastrin levels found in chagasic patients with the digestive clinical form has been attributed to the low gastric acid secretion caused by a partial denervation of the stomach, thereby affecting the parasympathetic stimulation 4-8 .The role of vagal nerves in the stimulation of gastric acid secretion in humans is complex, but it is known that acetylcholine released from these nerves has a direct stimulatory effect on parietal cells 14 . It has been demonstrated that acid hyposecretion and stomach nervous tissue injury are present mainly in the digestive form of Chagas disease. Both conditions can also occur in the cardiodigestive form, as patients presenting with either of these clinical forms of Chagas disease showed significant higher levels of gastrin in comparison with controls. Notably, the cardiodigestive form showed wide values of basal serum gastrin, but without significant difference with the digestive form.

Other mechanisms could also be involved in the increase of serum gastrin levels observed among chagasic patients such as reduced somatostatin secretion, because the significant decrease in the number of antral D-cells observed in CD patients could lead to hypergastrinemia in these patients. Likewise, patients infected with H. pylori have abnormal regulation of gastrin secretion due to lower somatostatin concentrations 16-19 in the antral region of the stomach. Moreover, the eradication of H. pylori results in the resolution of the gastritis and associated abnormalities 17,18,20 .

A high prevalence of coinfection with H. pylori was observed among patients with Chagas disease in this study (approximately 78%) and in others, irrespective of the clinical form of the disease 21-26 . In the histological evaluation, we observed that H. pylori infection was spread into the antral and corpus of gastric mucosa in the majority of patients with the digestive form of Chagas disease, which may also contribute to gastric atrophy, hypochlorydria and hypergastrinemia 26 . Thus, the hypergastrinemia that occurs in H. pylori -infected individuals has been attributed to hypochlorhydria, decreased secretion of somatostatin associated with the production of pro-inflammatory cytokines such as IL8, IL1β and TNFα, and also by a putative direct induction of the gastrin gene expression by the bacterium itself, especially the more virulent strains 10,13,27 .

Taking into account all these considerations, H. pylori gastric infection can contribute to the hypergastrinemia found in Chagas disease. In the present study, we demonstrated, using a logistic regression analysis, that H. pylori infection was not a confounding factor in the increased gastrin levels results observed in Chagas disease.

The gastric physiological changes that could be caused by a long term coinfection with H. pylori and T. cruzi are completely unknown, but various effects and individual variations can be expected. Therefore, further studies are necessary to clarify the real contribution of the bacterium in the elevated gastrin levels, in chronic Chagas disease. The treatment of chagasic patients coinfected with H. pylori and the measurement of their basal gastrin levels after the eradication of the bacterium could bring some highlights on this matter. Among non-chagasic patients, it has been demonstrated that the level of gastrin decreases after H. pylori eradication therapy 17,18,20,28 .

A better understanding of the real influence of H. pylori infection on gastrin levels in Chagas disease should lead to an improved management of coinfected patients. Furthermore, as chagasic patients usually have various dyspeptic symptoms and are H. pylori coinfected, the diagnosis of H. pylori infection and the corresponding eradication therapy should be considered in order to reduce the gastrointestinal symptoms and probably gastrin levels. Pinazzo et al . 24 reported the resolution or improvement of upper gastrointestinal symptoms in more than 70% of chagasic patients treated for H. pylori infection resulting in a clearer delineation of symptoms directly induced by T. cruzi infection.

The findings of the present study showed that basal serum gastrin levels are higher in patients with Chagas disease than in the non-chagasic ones (controls) even after adjustment for potential confounding factors such as age, gender and HP infection. Additionally, it was demonstrated, for the first time, that hypergastrinemia is present in the cardiodigestive clinical form of Chagas disease as well as in the digestive form.

ACKNOWLEDGMENTS

We thank Dr. Rodrigo Cunha de Sousa for his assistance with the analysis of patients’ clinical data.

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FUNDING

This work was supported by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil.

Received: October 2, 2018; Accepted: December 10, 2018

Correspondence to: Adriana Gonçalves de Oliveira Universidade Federal do Triangulo Mineiro, Instituto de Ciências Biológicas e Naturais, Praça Manoel Terra, 330, CEP 38025-180, Uberaba, MG, Brazil E-mail: adriana.oliveira@uftm.edu.br

CONFLICT OF INTERESTS

The authors declare no conflict of interests.

AUTHORS’ CONTRIBUTIONS

Jacqueline Batista Sousa participated in the anti- H. pylori IgG detection, collection of demographic data and clinical patient interviews and contributed to the writing of the manuscript; Renata Margarida Etchebehere, is an experienced pathologist who analyzed samples from the gastric mucosa; Dulciene Maria de Magalhães Queiroz analyzed data, performed the statistical analyses and made critical revisions; Fernanda Machado Fonseca participated in the collection of demographic data and clinical data from patients through interviews and analysis of medical records; Bianca Bontempi Batista participated in the urea breath test; Iracema Saldanha Junqueira participated in the esophagogastroduodenoscopy procedure; Sílvia Maria Perrone Camilo participated in patients’ follow-up; Adriana Gonçalves de Oliveira conceived, designed the study, analyzed data and drafted the manuscript. All authors read and approved the final manuscript.

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