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Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682

Rev. Soc. Bras. Med. Trop. vol.2 no.5 Uberaba Oct. 1968

http://dx.doi.org/10.1590/S0037-86821968000500003 

Rendimento dos meios seletivos para bactérias do gênero Fusobacterium

 

 

Wilson C. de AraújoI; Maria Raquel dos SantosII; Paulo FreitasIII

ILaboratório de Microbiologia Oral, Inst tituto de Microbiologia da U.F.R.J. - Professor Adjunto e Docente Livre da U.F.R.J.
IIProfessor Assistente -da F.O. da U.F. R.G. Norte. Bolsista da CAPES
IIIProfessor Assistente da F.O. da U.F.R.G. Sul. Bolsist a da CAPES

 

 


RESUMO

Meios seletivos para Fusobacterium foram, comparados no aspecto de sua eficiência no isolamento dos microrganismos fusiformes e quanto à inibição da flora concomitante. Material não calcificado do sulco gengival foi homogeneizado e diluído em tampão de fosfato (0,067 M - pH 7,2); 0,1 ml foi semeado em agar sangue, no meio de Omata & Disraely, meio de Omata & Disraely sem sôro, meio de McMarthy & Snyder, agar sangue com vancomicina, meio de Onisi, meio de Onisi com sôro de cavalo e meio de Onisi com pH 7,0. Em todos os casos a incubação foi realizada em anaerobiose, em jarra tipo Brewer, com 95% nitrogênio e 5% gás carbônico, durante 4 dias.
O meio de McCarthy & Snyder mostrou-se tão eficiente quanto o agar sangue em relação ao isolamento de Fusobacterium, ainda inibindo a flora concomitante, o meio de Omata & Disraely, com cristal violeta e estreptomicina, foi de certo modo inibidor também para Fusobacterium. O meio de Onisi, sugerido, sem sôro, possibilitou o isolamento de pequeno número de fusobactérias, provàvelmente as amostras não exigentes de proteínas naturais.


ABSTRACT

Gingival debris was collected with sterile periodontal scalers from patients with healthy gingiva and from periodontally involved patients to compare the effectiveness of selective media for isolation of Fusobacterium.
The material was weighed out on aluminum foil (previously weighed), homogenized, diluted into phosphate buffer (0,067 M-pH1,2), and 0,1 ml was plated out on blood agar, blood ugar-vancomycin (7,5 ug/ml), Omata & Disraely agar, Omata & Disraely agar without horse serum, McCarthy & Disraely agar, Onisi agar with horse serum, and Onisi agar with pH 7,2. In all cases the plates were incubated at 37°C, in anaerobic Brewer jars with 95% nitrogen and 5% carbon dioxide for fodr days.
McCarthy & Snyder agar was so effective as the plain blood agar in relation to the isolation of Fusobacterium; on the other hand, it inhibited the concomitant flora. Omata & Disraely agar (with crystal violet and streptomycin) was inhibitory for fusobacteria, too. A few number of fusobacteria was isolated in the Onisi medium (without serum) . Probably those strains did not require natural proteins.


 

 

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BIBLIOGRAFIA

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Trabalho realizado com auxilio do Conselho de Pesquisas da U.F.R.J.

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