Investigation of the response to the enterobacterial common antigen after typhoid vaccination

Milhomem, Arlete M.; Suassuna, ítalo

doi:10.1590/S0037-86821987000100002

Abstracts

Antibodies against the Salmonella typhi enterobacterial common antigen (ECA) and the O and H antigens were investigated in sera from healthy male subjects who had been previously vaccinated with the typhoid vaccine. No serological response to ECA was observed. Sera from subjects not previously vaccinated presented titers of ECA hemagglutinins which quantitatively were related to the presence ofH titers, but not to O agglutinins but with no statistical significance. The results are discussed in relation to the possible protective immunological mechanisms in typhoid fever.

Typhoid fever; Vaccine; Salmonella typhi; Enterobacterial common antigen; O and H antigens

Anticorpos contra o antígeno comum de enterobactérias (ECA) bem como contra os antigenos somáticos (O) e flagelar (H) de Salmonella typhi foram investigados no soro de recrutas do sexo masculino, após a vacinação. Não fo i detectada resposta humoral para ECA. Os soros obtidos antes da vacinação mostraram hemaglutininas para ECA acompanhando a presença de aglutininas para o antígeno H, ao contrário do que se observou em relação ao antígeno O. Discutem-se os resultados quanto ao possível mecanismo da imunoproteção da febre tifóide.

Febre tifóide; Vacinação; Antigenos comuns de enterobactérias e O e H de Salmonella typhi

ARTICLES

Investigation of the response to the enterobacterial common antigen after typhoid vaccination

Arlete M. Milhomem^I; ítalo Suassuna^II

^IInstituto de Microbiologia, Universidade Federal do Rio de Janeiro, RJ, Brasil

^IIFacuidade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, RJ, Brasil

ABSTRACT

Antibodies against the Salmonella typhi enterobacterial common antigen (ECA) and the O and H antigens were investigated in sera from healthy male subjects who had been previously vaccinated with the typhoid vaccine. No serological response to ECA was observed. Sera from subjects not previously vaccinated presented titers of ECA hemagglutinins which quantitatively were related to the presence ofH titers, but not to O agglutinins but with no statistical significance. The results are discussed in relation to the possible protective immunological mechanisms in typhoid fever.

Keywords: Typhoid fever. Vaccine. Salmonella typhi. Enterobacterial common antigen. O and H antigens.

RESUMO

Anticorpos contra o antígeno comum de enterobactérias (ECA) bem como contra os antigenos somáticos (O) e flagelar (H) de Salmonella typhi foram investigados no soro de recrutas do sexo masculino, após a vacinação. Não fo i detectada resposta humoral para ECA. Os soros obtidos antes da vacinação mostraram hemaglutininas para ECA acompanhando a presença de aglutininas para o antígeno H, ao contrário do que se observou em relação ao antígeno O. Discutem-se os resultados quanto ao possível mecanismo da imunoproteção da febre tifóide.

Palavras-chave: Febre tifóide. Vacinação. Antigenos comuns de enterobactérias e O e H de Salmonella typhi.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 10/3/86.

1. Arkwright JA. The value of different kinds of antigen in prophylactic enteric vaccines. Journal of Pathology and Bacteriology 30:345-364, 1927.
2. Barber C, Eylan E. Behaviour of salmonellae non-pathogenic for mice. I. Neutralization of their toxicities by induced antiprotein antibodies. Zentralblatt furBakteriologie und Parasitenkund Infektionskrankheiten und Hygiene. Abteilung Originale Reihe A 237:213-221,1977.
3. Beumer J. Les vaccins antibacteriens: des microbes vivants aux antigčne purifiés. Bulletin de lTnstitute Pasteur 72:35-48, 1974.
4. Berquô ES, Souza JMP, Gotlieb SLD. Bioestatistica. Editora Pedagógica e Universitária Ltda. S. Paulo, 1980.
5. Colins FM. Mechanisms in antimicrobial immunity. Journal of the Reticuloendothelial Society 10:58-99, 1971.
6. Domingue GJ, Sahli A, Rountree C, Little W. Prevention of experimental hematogenous and retrograde pyelonephritis by antibodies against enterobacterial common antigen. Infection and Immunity 2:175-182, 1970.
7 Doshi N, Taylor AG. Comparison of the Vi indirect fluorescent antibody test with the Widal agglutination method in the serodiagnosis of typhoid fever. Journal of Clinical Pathology 37:805-808, 1984.
8. Edwards EA, Johnson DP, Pierce WE, Peckinpaugh RO. Reactions and sorologic responses to monovalent acetone-inactivated typhoid vaccine and heat-killed TAB when given by jet injection. Bulletin of the World Health Organization 51:501-505, 1974.
9. Edwards PR, Ewing WH. Identification of Enterobacteriaceae 3^rd ed. Burgess Publishing Co. Minneapolis, Minessota, 1972.
10. Felix A, Pitt RM. A new antigen of B. typhosus Its relation to virulence and to active and passive immunization. Lancet 2:186-191, 1934.
11. Gorzynski EA, van Oss CJ, Ambrus JL, Neter E. The hemagglutinin response of human subjects to common enterobacterial antigen. Infection and Immunity 5:625626, 1972.
12. Gorzynski EA. Cross-reactivity between mouse tissue and enterobacterial common antigen (CA). Military Medicine 141:610-612, 1976.
13. Kunin CM, Beard MV, Halmaguy NE. Evidence for a common hapten associated with endotoxin fraction of Escherichia coli and other Enterobacteriaceae Proceedings of the Society for Experimental Biology and Medicine 111:160-166, 1962.
14. Levine MM, Kaper JB, Black RE, Clements Ml. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiology Reviews 47:510-550, 1985.
15. Lugowski C, Kulakowska M, Romanowska E. Enterobacterial common antigen-tetanus toxoide conjugate as immunogen. Infection and Immunity 42:1086-1091, 1983.
16. Mŕkela PH, Mayer H. Enterobacterial common antigen. Bacteriological Reviews 40:591-632, 1976.
17. Marx A, PetcoviciM, NacescuN, Mayer H, Schmidt G. Demonstration of enterobacterial common antigen by bacterial agglutination. Infection and Immunity 18:563567, 1977.
18. McLaughlin JC, Domingue GL. The immunologic role of the ethanol-soluble enterobacterial common antigen versus experimental renal infection. Immunological Communications 3: 51-75, 1974.
19. Milhomem AM, Suassuna I. A imunidade na febre tifóide. I. A vacinaçăo antitifóidica de Wright, 1896 a 1979. Memórias do Instituto Oswaldo Cruz 77:93-120, 1982.
20. Milhomem AM, Suassuna I. A imunidade na febre tifóide. II. Antígenos e resposta imune nas salmoneloses sistęmicas. Memórias do Instituto Oswaldo Cruz 77.453483, 1982.
21. Morgenstern MA, Gorzynski EA. Immune response of guinea-pigs to common enterobacterial antigen. Immu-nological Communications 2:495-506, 1973.
22. Neter E, WhangHY, Luderitz O, Westphal O. Immunological priming without production of circulating bacterial antibodies conditioned by endotoxin and its lipoid A component. Nature 212:420-421, 1966.
23. Pessôa MHR, Suassuna I. Relaçăo de hemaglutinaçăo passiva e aglutinaçăo bacteriana: influęncia da natureza do antigeno, classe de imunoglobulinas e seu emprego. Revista Brasileira de Patologia Clínica 16:12-17, 1980.
24. Polish Typhoid Committee. Evaluation of typhoid vaccines in the laboratory and in controlled field trial in Poland (Preliminary report). Bulletin of the World Health Orga-nization 32:15-27, 1965.
25. Suassuna I, Nogueira ES, Fagundes CF. Títulos significativos da reaçăo de Widal para o diagnóstico de salmoneloses no Estado da Guanabara. Revista Médica da Aeronáutica 1-2:123-130, 1970.
26. Suzuki T, Gorzynski EA, Neter E. Separation by ethanol of common and somatic antigens of Enterobacteriaceae Journal of Bacteriology 88:1240-1243, 1964.
27. Venneman MR. Purification of immunologically active ribonucleic acid preparations of Salmonella typhimurium: molecular-sieve and anion-exchange chromatography. Infection and Immunity 5:269-282, 1972.
28. Whang HY, Neter E. Immunological studies of heterogenetic enterobacterial antigen (Kunin). Journal of Bacteriology 84:1245-1250, 1962.
29. Whang HY, Neter E. Further studies on effect of endotoxin on antibody response of rabbit to common antigen of Enterobacteriaceae Journal of Immunology 98:948956, 1967.
30. Woodward WE. Volunteer studies of typhoid fever and vaccines. Transactions of the Royal Society of Tropical Medicine and Hygiene 74:553-556. 1980.

Publication Dates

Publication in this collection
06 June 2013
Date of issue
Mar 1987

History

Received
10 Mar 1986

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Arkwright JA. The value of different kinds of antigen in prophylactic enteric vaccines. Journal of Pathology and Bacteriology 30:345-364, 1927.

[2] 2. Barber C, Eylan E. Behaviour of salmonellae non-pathogenic for mice. I. Neutralization of their toxicities by induced antiprotein antibodies. Zentralblatt furBakteriologie und Parasitenkund Infektionskrankheiten und Hygiene. Abteilung Originale Reihe A 237:213-221,1977.

[3] 3. Beumer J. Les vaccins antibacteriens: des microbes vivants aux antigčne purifiés. Bulletin de lTnstitute Pasteur 72:35-48, 1974.

[4] 4. Berquô ES, Souza JMP, Gotlieb SLD. Bioestatistica. Editora Pedagógica e Universitária Ltda. S. Paulo, 1980.

[5] 5. Colins FM. Mechanisms in antimicrobial immunity. Journal of the Reticuloendothelial Society 10:58-99, 1971.

[6] 6. Domingue GJ, Sahli A, Rountree C, Little W. Prevention of experimental hematogenous and retrograde pyelonephritis by antibodies against enterobacterial common antigen. Infection and Immunity 2:175-182, 1970.

[7] 7 Doshi N, Taylor AG. Comparison of the Vi indirect fluorescent antibody test with the Widal agglutination method in the serodiagnosis of typhoid fever. Journal of Clinical Pathology 37:805-808, 1984.

[8] 8. Edwards EA, Johnson DP, Pierce WE, Peckinpaugh RO. Reactions and sorologic responses to monovalent acetone-inactivated typhoid vaccine and heat-killed TAB when given by jet injection. Bulletin of the World Health Organization 51:501-505, 1974.

[9] 9. Edwards PR, Ewing WH. Identification of Enterobacteriaceae 3^rd ed. Burgess Publishing Co. Minneapolis, Minessota, 1972.

[10] 10. Felix A, Pitt RM. A new antigen of B. typhosus Its relation to virulence and to active and passive immunization. Lancet 2:186-191, 1934.

[11] 11. Gorzynski EA, van Oss CJ, Ambrus JL, Neter E. The hemagglutinin response of human subjects to common enterobacterial antigen. Infection and Immunity 5:625626, 1972.

[12] 12. Gorzynski EA. Cross-reactivity between mouse tissue and enterobacterial common antigen (CA). Military Medicine 141:610-612, 1976.

[13] 13. Kunin CM, Beard MV, Halmaguy NE. Evidence for a common hapten associated with endotoxin fraction of Escherichia coli and other Enterobacteriaceae Proceedings of the Society for Experimental Biology and Medicine 111:160-166, 1962.

[14] 14. Levine MM, Kaper JB, Black RE, Clements Ml. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiology Reviews 47:510-550, 1985.

[15] 15. Lugowski C, Kulakowska M, Romanowska E. Enterobacterial common antigen-tetanus toxoide conjugate as immunogen. Infection and Immunity 42:1086-1091, 1983.

[16] 16. Mŕkela PH, Mayer H. Enterobacterial common antigen. Bacteriological Reviews 40:591-632, 1976.

[17] 17. Marx A, PetcoviciM, NacescuN, Mayer H, Schmidt G. Demonstration of enterobacterial common antigen by bacterial agglutination. Infection and Immunity 18:563567, 1977.

[18] 18. McLaughlin JC, Domingue GL. The immunologic role of the ethanol-soluble enterobacterial common antigen versus experimental renal infection. Immunological Communications 3: 51-75, 1974.

[19] 19. Milhomem AM, Suassuna I. A imunidade na febre tifóide. I. A vacinaçăo antitifóidica de Wright, 1896 a 1979. Memórias do Instituto Oswaldo Cruz 77:93-120, 1982.

[20] 20. Milhomem AM, Suassuna I. A imunidade na febre tifóide. II. Antígenos e resposta imune nas salmoneloses sistęmicas. Memórias do Instituto Oswaldo Cruz 77.453483, 1982.

[21] 21. Morgenstern MA, Gorzynski EA. Immune response of guinea-pigs to common enterobacterial antigen. Immu-nological Communications 2:495-506, 1973.

[22] 22. Neter E, WhangHY, Luderitz O, Westphal O. Immunological priming without production of circulating bacterial antibodies conditioned by endotoxin and its lipoid A component. Nature 212:420-421, 1966.

[23] 23. Pessôa MHR, Suassuna I. Relaçăo de hemaglutinaçăo passiva e aglutinaçăo bacteriana: influęncia da natureza do antigeno, classe de imunoglobulinas e seu emprego. Revista Brasileira de Patologia Clínica 16:12-17, 1980.

[24] 24. Polish Typhoid Committee. Evaluation of typhoid vaccines in the laboratory and in controlled field trial in Poland (Preliminary report). Bulletin of the World Health Orga-nization 32:15-27, 1965.

[25] 25. Suassuna I, Nogueira ES, Fagundes CF. Títulos significativos da reaçăo de Widal para o diagnóstico de salmoneloses no Estado da Guanabara. Revista Médica da Aeronáutica 1-2:123-130, 1970.

[26] 26. Suzuki T, Gorzynski EA, Neter E. Separation by ethanol of common and somatic antigens of Enterobacteriaceae Journal of Bacteriology 88:1240-1243, 1964.

[27] 27. Venneman MR. Purification of immunologically active ribonucleic acid preparations of Salmonella typhimurium: molecular-sieve and anion-exchange chromatography. Infection and Immunity 5:269-282, 1972.

[28] 28. Whang HY, Neter E. Immunological studies of heterogenetic enterobacterial antigen (Kunin). Journal of Bacteriology 84:1245-1250, 1962.

[29] 29. Whang HY, Neter E. Further studies on effect of endotoxin on antibody response of rabbit to common antigen of Enterobacteriaceae Journal of Immunology 98:948956, 1967.

[30] 30. Woodward WE. Volunteer studies of typhoid fever and vaccines. Transactions of the Royal Society of Tropical Medicine and Hygiene 74:553-556. 1980.