Studies on anti-component 5 antibodies in animals infected with Trypanosoma cruzi

Santoro, F.; Sadigursky, M.; Sherlock, I.; Afchain, D.; Capron, A.

doi:10.1590/S0037-86821987000200005

Abstracts

A competitive antibody enzyme immunoassay, using a monoclonal antibody against the species-specific Trypanosoma crnzi antigen 5, was used to investigate the presence of anti-component 5 antibodies in sera of opossums, dogs, rabbits and rats infected with this parasite. The sera from 51 Venezuelan patients with Chagas’disease were also tested. About 90% of the infected subjects showed significant levels of anti-component 5 antibodies. Nevertheless, these antibodies were not detected in the sera of dogs, rats and opossums infected with T. cruzl Some sera from infected rabbits presented significant results but close to the limit ofpositivity ofthe test. These findings suggest that the immune response in animals naturally or experimentally infected with T. cruzi is different from that observed in human Chagas’disease.

Trypanosoma cruzi; Antibodies; Immunoassay

Foi usado um teste imunoenzimático competitivo para investigar a presença de anticorpos anticomponente 5 nos soros de sariguês, cães, coelhos e ratos infectados com o Trypanosoma cruzi. Neste teste, foi utilizado um anticorpo monoclonal contra o antígeno 5 que é específico do T. cruzi. Também foram testados os soros de 51 pacientes venezuelanos com doença de Chagas. Apesar desses anticorpos não serem detectados nos soros de cães, ratos e sariguês infectados com o T. cruzi, alguns soros de coelhos infectados apresentaram resultados positivos porém em níveis próximos aos do limite de positividade do teste. Esses achados sugerem que a resposta imune em animais naturalmente ou experimentalmente infectados com o T. cruzi é diferente daquela que é observada na doença de Chagas humana.

Trypanosoma cruzi; Anticorpos; Imuno-ensaio

ARTICLES

Studies on anti-component 5 antibodies in animals infected with Trypanosoma cruzi

F. Santoro^I; M. Sadigursky^II; I. Sherlock^II; D. Afchain^I; A. Capron^I

^ICentre dimmunologie et de Biologie Parasitaire (Unité Mixte INSERM U 167 - CNRS 624), Institut Pasteur, 1, rue du Prof. A. Calmette, 59019 LILLE Cédex, France

^IICentro de Pesquisas Gonçalo Moniz (FIOCRUZ/UFBa), 40000 Salvador, Bahia, Brazil

^{Adress to correspondence} Adress to correspondence: Dr. Reinaldo Martinelli. FACP/ Laboratório de Nefrologia - 1117 Hospital Universitário Prof. Edgard Santos. Rua João das Botas s/n, Canela 40110-160 Salvador, BA, Brasil.

ABSTRACT

A competitive antibody enzyme immunoassay, using a monoclonal antibody against the species-specific Trypanosoma crnzi antigen 5, was used to investigate the presence of anti-component 5 antibodies in sera of opossums, dogs, rabbits and rats infected with this parasite. The sera from 51 Venezuelan patients with Chagasdisease were also tested. About 90% of the infected subjects showed significant levels of anti-component 5 antibodies. Nevertheless, these antibodies were not detected in the sera of dogs, rats and opossums infected with T. cruzl Some sera from infected rabbits presented significant results but close to the limit ofpositivity ofthe test. These findings suggest that the immune response in animals naturally or experimentally infected with T. cruzi is different from that observed in human Chagasdisease.

Keywords:Trypanosoma cruzi. Antibodies. Immunoassay.

RESUMO

Foi usado um teste imunoenzimático competitivo para investigar a presença de anticorpos anticomponente 5 nos soros de sariguês, cães, coelhos e ratos infectados com o Trypanosoma cruzi. Neste teste, foi utilizado um anticorpo monoclonal contra o antígeno 5 que é específico do T. cruzi. Também foram testados os soros de 51 pacientes venezuelanos com doença de Chagas.

Apesar desses anticorpos não serem detectados nos soros de cães, ratos e sariguês infectados com o T. cruzi, alguns soros de coelhos infectados apresentaram resultados positivos porém em níveis próximos aos do limite de positividade do teste. Esses achados sugerem que a resposta imune em animais naturalmente ou experimentalmente infectados com o T. cruzi é diferente daquela que é observada na doença de Chagas humana.

Palavras-chave:Trypanosoma cruzi. Anticorpos. Imuno-ensaio.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 19/11/92.

Department of Medicine, Federal University of Bahia Medical School, Salvador BA Brazil.

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq, Brazil (Grant n° 407670/85CL).

1. Afchain D, Capron A, Prata A. Les anticorps précipitantes dans la trypanosomiase humaine. Gazeta Médica da Bahia 70: 141-147, 1970.
2. Afchain D, Fruit J, Yarzabal L, Capron A. Purification of a specific antigen of Trypanosoma cruzi from the culture forms. American Journal of Tropical Medicine and Hygiene 27:478-482, 1978.
3. Afchain D, Le Ray D, Fruit J, Capron A. Antigenic make-up of Trypanosoma cruzi culture forms: identification of a specific component Journal of Parasitology 65:507-514, 1979.
4. Andrade ZA, Andrade SG, Sadigursky M. Damage and healing in the conducting tissue of the heart (an experimental study in dogs infected with Trypanosoma cruzi). Journal of Pathology 143:93-101, 1984.
5. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6: 43-52, 1969.
6. Barreto MP. Epidemiologia. In: Trypanosoma cruzi e Doença de Chagas.Brener Z & Andrade Z (eds.), Guanabara Koogan SA, Rio de Janeiro pp. 89-151, 1979.
7. Breničre FS, Carrasco R, Moinedo S, Lemesre JL, Desjeux P, Afchain D, Carlier Y. Specific immunodiagnosis of Chagasdisease: immunodiffusion test using a specific serum anti -Trypanosoma cruzi component 5. Tropical and Geographical Medicine, in press.
8. Bronzina AA, DAlessandro A, Segura E. Différencias y similitudes antigenicas entre Trypanosoma rangeli y Trypanosoma cruzi Medicina (Buenos Aires) 40:4549, 1980.
9. Camargo ME, Rebonato C. Cross-reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies. A simple inhibition procedure to ensure specific results. American Journal of Tropical Medicine and Hygiene 18:500-505, 1969.
10. Cerisola JA, Alvarez M, Bock M, Wegner D. A comparison of a new antigen from amastigotes of Trypanosoma cruzi and an antigen from epimastigotes for the diagnosis of Chagasdisease by the indirect immunofluorescence test. Revista do Instituto de Medicina Tropical de Săo Paulo 13:162-166, 1971.
11. DAlessandro A. Biology of Trypanosoma (Herpetosoma) rangeli. In: Biology of the Kinetoplastida. Lumsden WHR & Evans DA, (ed.), Academic Press, London pp. 328-403, 1979.
12. Desjeux P, Quilici M, Lapierre J. A propos de 113 cas de leishmaniose cutanée et cutanéo-muqueuse observés en Bolivie. Etude séro-immunologique de 71 cas. Bulletin de la Société de Pathologie Exotique 67:387-395, 1974.
13. Desjeux P, Aranda E, Aliago O, Mollinedo S. Human visceral leishmaniasis in Bolivia: first proven case from Los Jungas. Transactions of the Royal Society of Tropical Medicine and Hygiene 77:851-852, 1983.
14. Guimarăes MC, Celeste BJ, Ayres EC, Mineo JR, Diniz JMP. Immunoenzymatic assay (Elisa) in mucocutaneous leishmaniasis, Kala-azar and Chagasdisease: an epimastigote Trypanosoma cruzi antigen able to distinguish anti -Trypanosoma and anivLeishmania antibodies. American Journal of Tropical Medicine and Hygiene 30:942-947, 1981.
15. Kirchhoff LV, Engel JC, Dvorak JA, Sher A. Strains and clones of Trypanosoma cruzi differ in their expression of a surface antigen identified by a monoclonal antibody. Molecular and Biochemical Parasitology 11:81-89, 1984.
16. Lemesre JL, Afchain D, Orozco O, Loyens M, Breničre FS, Desjeux P, Carlier Y, Martin V, Nogueira-Queiroz J.A, Le Ray D, Capron A. Specific and sensitive immunological diagnosis of Chagasdisease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi specific monoclonal antibody. American Journal of Tropical and Medicine and Hygiene 35:8693, 1986.
17. Lemesre JL, Rizvi FS, Afchain D, Sadigursky M, Capron A, Santoro F. Subspecies-specific surface antigens of the Leishmania donovani complex. Infection and Immunity 50:136-141, 1985.
18. Orozco O, Afchain D, Rodriguez C, Ovlaque G, Loyens M, Capron A. Production dun anticorps monoclonal anti-antigčne 5 de Trypanosoma cruzi Compterendus de lAcadémie des Sciences de Paris (Série III) 295:783-785, 1982.
19. Orozco O, Afchain D, Dissous C, Rodriguez C, Ovlaque G, Lemesre JL, Loyens M, Capron A. Different monoclonal antibodies against the component 5 specific for Trypanosoma cruzi American Journal of Tropical Medicine and Hygiene 33:560-568, 1984.
20. Rodriguez AM, Santoro F, Afchain D, Bazin H, Capron A. Trypanosoma cruzi infection in B-cell- deficient rats. Infection and Immunity 31:524-529, 1981.
21. Scharfstein J, Rodrigues MM, Andrade-AIves C, Souza W, Previato JO, Mendonça-Previato L. Trypanosoma cruzi: description of a highly purified surface antigen defined by human antibodies. Journal ofimmunology 131:972-976, 1983.
22. Schechter, M, Voller, A, Marinkelle CJ, Flint JE, Guhl F, Miles MA. Purified Trypanosoma cruzi specific glycoprotein for discriminative serological diagnosis of south American tripanosomiasis (Chagasdisease), Lancet 2:934-941, 1983.
23. Souza OE, Johnson CM. Frequency and distribution of Trypanosoma cruzi in the Republic of Panama. American Journal of Tropical Medicine and Hygiene 20: 405-410, 1971.

Adress to correspondence:

Dr. Reinaldo Martinelli.

FACP/ Laboratório de Nefrologia - 1117

Hospital Universitário

Prof. Edgard Santos.

Rua João das Botas s/n, Canela

40110-160

Salvador, BA, Brasil.

Publication Dates

Publication in this collection
05 June 2013
Date of issue
June 1987

History

Received
19 Nov 1992

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Afchain D, Capron A, Prata A. Les anticorps précipitantes dans la trypanosomiase humaine. Gazeta Médica da Bahia 70: 141-147, 1970.

[2] 2. Afchain D, Fruit J, Yarzabal L, Capron A. Purification of a specific antigen of Trypanosoma cruzi from the culture forms. American Journal of Tropical Medicine and Hygiene 27:478-482, 1978.

[3] 3. Afchain D, Le Ray D, Fruit J, Capron A. Antigenic make-up of Trypanosoma cruzi culture forms: identification of a specific component Journal of Parasitology 65:507-514, 1979.

[4] 4. Andrade ZA, Andrade SG, Sadigursky M. Damage and healing in the conducting tissue of the heart (an experimental study in dogs infected with Trypanosoma cruzi). Journal of Pathology 143:93-101, 1984.

[5] 5. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6: 43-52, 1969.

[6] 6. Barreto MP. Epidemiologia. In: Trypanosoma cruzi e Doença de Chagas.Brener Z & Andrade Z (eds.), Guanabara Koogan SA, Rio de Janeiro pp. 89-151, 1979.

[7] 7. Breničre FS, Carrasco R, Moinedo S, Lemesre JL, Desjeux P, Afchain D, Carlier Y. Specific immunodiagnosis of Chagasdisease: immunodiffusion test using a specific serum anti -Trypanosoma cruzi component 5. Tropical and Geographical Medicine, in press.

[8] 8. Bronzina AA, DAlessandro A, Segura E. Différencias y similitudes antigenicas entre Trypanosoma rangeli y Trypanosoma cruzi Medicina (Buenos Aires) 40:4549, 1980.

[9] 9. Camargo ME, Rebonato C. Cross-reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies. A simple inhibition procedure to ensure specific results. American Journal of Tropical Medicine and Hygiene 18:500-505, 1969.

[10] 10. Cerisola JA, Alvarez M, Bock M, Wegner D. A comparison of a new antigen from amastigotes of Trypanosoma cruzi and an antigen from epimastigotes for the diagnosis of Chagasdisease by the indirect immunofluorescence test. Revista do Instituto de Medicina Tropical de Săo Paulo 13:162-166, 1971.

[11] 11. DAlessandro A. Biology of Trypanosoma (Herpetosoma) rangeli. In: Biology of the Kinetoplastida. Lumsden WHR & Evans DA, (ed.), Academic Press, London pp. 328-403, 1979.

[12] 12. Desjeux P, Quilici M, Lapierre J. A propos de 113 cas de leishmaniose cutanée et cutanéo-muqueuse observés en Bolivie. Etude séro-immunologique de 71 cas. Bulletin de la Société de Pathologie Exotique 67:387-395, 1974.

[13] 13. Desjeux P, Aranda E, Aliago O, Mollinedo S. Human visceral leishmaniasis in Bolivia: first proven case from Los Jungas. Transactions of the Royal Society of Tropical Medicine and Hygiene 77:851-852, 1983.

[14] 14. Guimarăes MC, Celeste BJ, Ayres EC, Mineo JR, Diniz JMP. Immunoenzymatic assay (Elisa) in mucocutaneous leishmaniasis, Kala-azar and Chagasdisease: an epimastigote Trypanosoma cruzi antigen able to distinguish anti -Trypanosoma and anivLeishmania antibodies. American Journal of Tropical Medicine and Hygiene 30:942-947, 1981.

[15] 15. Kirchhoff LV, Engel JC, Dvorak JA, Sher A. Strains and clones of Trypanosoma cruzi differ in their expression of a surface antigen identified by a monoclonal antibody. Molecular and Biochemical Parasitology 11:81-89, 1984.

[16] 16. Lemesre JL, Afchain D, Orozco O, Loyens M, Breničre FS, Desjeux P, Carlier Y, Martin V, Nogueira-Queiroz J.A, Le Ray D, Capron A. Specific and sensitive immunological diagnosis of Chagasdisease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi specific monoclonal antibody. American Journal of Tropical and Medicine and Hygiene 35:8693, 1986.

[17] 17. Lemesre JL, Rizvi FS, Afchain D, Sadigursky M, Capron A, Santoro F. Subspecies-specific surface antigens of the Leishmania donovani complex. Infection and Immunity 50:136-141, 1985.

[18] 18. Orozco O, Afchain D, Rodriguez C, Ovlaque G, Loyens M, Capron A. Production dun anticorps monoclonal anti-antigčne 5 de Trypanosoma cruzi Compterendus de lAcadémie des Sciences de Paris (Série III) 295:783-785, 1982.

[19] 19. Orozco O, Afchain D, Dissous C, Rodriguez C, Ovlaque G, Lemesre JL, Loyens M, Capron A. Different monoclonal antibodies against the component 5 specific for Trypanosoma cruzi American Journal of Tropical Medicine and Hygiene 33:560-568, 1984.

[20] 20. Rodriguez AM, Santoro F, Afchain D, Bazin H, Capron A. Trypanosoma cruzi infection in B-cell- deficient rats. Infection and Immunity 31:524-529, 1981.

[21] 21. Scharfstein J, Rodrigues MM, Andrade-AIves C, Souza W, Previato JO, Mendonça-Previato L. Trypanosoma cruzi: description of a highly purified surface antigen defined by human antibodies. Journal ofimmunology 131:972-976, 1983.

[22] 22. Schechter, M, Voller, A, Marinkelle CJ, Flint JE, Guhl F, Miles MA. Purified Trypanosoma cruzi specific glycoprotein for discriminative serological diagnosis of south American tripanosomiasis (Chagasdisease), Lancet 2:934-941, 1983.

[23] 23. Souza OE, Johnson CM. Frequency and distribution of Trypanosoma cruzi in the Republic of Panama. American Journal of Tropical Medicine and Hygiene 20: 405-410, 1971.